ライフサイエンスコーパス: K-Cl cotransporter

1 to the human colonic T84 epithelial cell Na-K-Cl cotransporter.

2 that it would be distantly related to the Na-K-Cl cotransporter.

3 o examine the effect on O2 dependency of the K+-Cl- cotransporter.

4 rter, and NKCC, the chloride accumulator Na+-K+-Cl- cotransporter.

5 nd increased gene expression of NKCC1--a Na+-K+-Cl- cotransporter.

6 ial homology to KCC2, the mammalian neuronal K(+)-Cl(-) cotransporter.

7 been suggested that the electroneutral Na(+)-K(+)-Cl(-) cotransporter 1 (NKCC1) can play a role in gl

8 The Na(+)-K(+)-Cl(-) cotransporter 1 (NKCC1) is particularly relev

9 Alternatively, inhibition of the Na(+)-K(+)-Cl(-) cotransporter 1 (NKCC1), a Cl(-) importer exp

10 is accompanied by down-regulation of the Na-K-Cl cotransporter 1 (Nkcc1) and the Ca(2+)-activated an

11 t colonic basolateral membrane suggests that K-Cl cotransporter: 1) is involved in transepithelial po

12 The VP neurons lacked expression of the K(+)-Cl(-) cotransporter 2 (KCC2), the predominant Cl(-)

13 he Na(+)-K(+)-2Cl(-) cotransporter 1 and the K(+)-Cl(-) cotransporter 2 (KCC2).

14 K(+)/Cl(-) cotransporter 2 (KCC2) is selectively express

15 Expression of the K+Cl- cotransporter 2 (KCC2) also increased across these

16 evidence indicates that the neuron-specific K-Cl cotransporter 2 (KCC2) is the major chloride extrus

17 and GABA(A) receptor alpha1 subunit, but not K-Cl cotransporter 2, are colocalized at the presumed po

18 a(+)-K(+)-2Cl(-) cotransporter-1 (NKCC1) and K(+)-Cl(-) cotransporter-2 (KCC2), critically influence

19 n the absence of water transport through the K(+)-Cl(-) cotransporter, a large osmotic gradient build

20 d with a monoclonal antibody to mammalian Na-K-Cl cotransporter and a fluorescent secondary antibody

21 mportant new insights into the regulation of K-Cl cotransporters and provides in vivo evidence that i

22 +)/Pi co-transporter 2, phosphorylated Na(+)/K(+)/Cl(-) cotransporter, and phosphorylated Na(+)/Cl(-)

23 sion patterns of KCC2, the chloride extruder K+-Cl- cotransporter, and NKCC, the chloride accumulator

24 P (sickle), Ca 2+ -sensitive K channel, and K:Cl cotransporter, and of therapies targeted at these t

25 in using bumetanide, a chloride importer Na-K-Cl cotransporter antagonist, for treatment of neurolog

26 Electroneutral Na-(K)-Cl cotransporters are present in most cell types, whe

27 The Na-K-Cl cotransporters are a class of ion transport protein

28 n chloride cotransporters, e.g. Na-K-2Cl and K-Cl cotransporters, are activated to maintain fluid/ion

29 Regulation of the K+-Cl- cotransporter by O2 is very similar in equine, sh

30 Expression of NKCC1, an Na-K-Cl cotransporter characteristic for ductal epithelia,

31 the expected functional characteristics of a K+Cl- cotransporter: Cl--dependent uptake of 86Rb+ which

32 Here we review what is known about the Na-(K)-Cl cotransporters cloned to date and what can be dedu

33 ced monoclonal antibody (T4) specific for Na-K-Cl cotransporter displays the same localization.

34 ing the chloride gradient by blocking the Na-K-Cl cotransporter eliminated responses to light moving

35 Two Na-K-Cl cotransporters encoded by different genes have been

36 KCC2 is a neuron-specific K(+)-Cl(-) cotransporter essential for establishing the

37 Na-K-Cl cotransporter fluorescence patterns showed more var

38 ve no effect on total KCC2 protein level and K-Cl cotransporter function.

39 The other members of this K(+)-Cl(-) cotransporter gene family are exclusively swe

40 KCC2 is the neuron-specific member of the of K(+)-Cl(-) cotransporter gene family.

41 Na(+)-K(+)-Cl(-) cotransporter has been proposed to play an im

42 mutations that reduce the level of the sole K+/Cl- cotransporter in the fruitfly Drosophila melanoga

43 m tight junctions supports a role for the Na-K-Cl cotransporter in ciliary epithelium as a chloride e

44 dertaken to determine the location of the Na-K-Cl cotransporter in fixed sections of bovine eye.

45 assing a total of seven Na-Cl, Na-K-2Cl, and K-Cl cotransporters, in addition to two related transpor

46 The pharmacology of the K-Cl cotransporter is dominated by loop diuretics such a

47 t the behavior of the endogenous HEK cell Na-K-Cl cotransporter is unlike either of the two forms whi

48 that pharmacological inhibition of the Na(+)-K(+)-Cl(-) cotransporter isoform 1 (NKCC1) during ischem

49 , which inhibit Cl- influx through the Na(+)-K(+)-Cl- cotransporter isoform-1 (NKCC-1) and efflux thr

50 er isoform-1 (NKCC-1) and efflux through the K(+)-Cl- cotransporter isoform-2, were unable to inhibit

51 2 is 67% identical to the widely distributed K-Cl cotransporter isoform (KCC1) identified in rat brai

52 ary screening, we have identified a putative K-Cl cotransporter isoform (KCC2) in rat brain that is s

53 at cation-dependent Cl- transport protein Na-K-Cl cotransporter isoform 1 (NKCC1) plays a role in the

54 Na-K-Cl cotransporter isoform 1 (NKCC1) plays an important

55 To date, two Na-K-Cl cotransporter isoforms have been identified: NKCC1,

56 o includes Na-Cl cotransporters and multiple K-Cl cotransporter isoforms.

57 While animals lacking ABTS-1 or the K(+)-Cl(-) cotransporter KCC-2 display only mild behavio

58 ed K(+) channel (the Gardos channel) and the K(+)-Cl(-) cotransporter (KCC) - it would have a synergi

59 The K(+):Cl(-) cotransporter (KCC) activity is modulated by

60 ects of CDNB (1 mM) on the activities of the K+-Cl- cotransporter (KCC; measured as Cl--dependent K+

61 s among the polypeptide products of the four K-Cl cotransporter (KCC) genes are little understood.

62 The K-Cl cotransporter (KCC) regulates red blood cell (RBC)

63 cells (VSMCs) express at least two mRNAs for K-Cl cotransporters (KCC): KCC1 and KCC3.

64 2Cl cotransporter NKCC1 and Cl(-) efflux via K-Cl cotransporters, KCC1 or KCC2.

65 The neuron-specific K(+)-Cl(-) cotransporter KCC2 plays a crucial role in de

66 Diminished levels of the K(+)/Cl(-) cotransporter KCC2 and a depolarizing GABAA r

67 The K(+)/Cl(-) cotransporter KCC2 is the main mechanism by w

68 d postsynaptic activity acts on the neuronal K+/Cl- cotransporter KCC2 to shift the reversal potentia

69 larger in mature neurons, which express the K-Cl cotransporter KCC2 at high levels, and inhibition o

70 The K-Cl cotransporter KCC2 establishes the low intraneurona

71 Targeted deletion of the neuronal-specific K-Cl cotransporter KCC2 generates mice with a profound s

72 pmental fall in [Cl(-)]i and the role of the K-Cl cotransporter KCC2 in this process.

73 The neuronal K-Cl cotransporter KCC2 maintains the low intracellular

74 The K-Cl cotransporter KCC2 plays a crucial role in neuronal

75 in Cl(-) homeostasis is the neuron-specific K(+)-Cl(-) cotransporter (KCC2).

76 The K(+)/Cl(-) cotransporter (KCC2) allows adult neurons to

77 n caused a progressive downregulation of the K+-Cl- cotransporter (KCC2), which may have contributed

78 The neuron-specific K-Cl cotransporter (KCC2) is hypothesized to function as

79 o test this, we immunostained retina for the K-Cl cotransporter (KCC2) that normally extrudes chlorid

80 ess-induced functional downregulation of the K(+), Cl(-) cotransporter, KCC2.

81 ted E(GABA), expression of the Cl- extruding K+/Cl- cotransporter, KCC2 was decreased.

82 ted that the protein product of Slc12a6, the K(+)-Cl(-) cotransporter Kcc3, was not detectable in gax

83 dates for Mendelian human disorders, and the K-Cl cotransporter KCC3, in particular, may be involved

84 EAAT1(P>R) mutation, the chloride-extruding K(+)-Cl(-) cotransporter KccB also caused astroglial mal

85 K(+)-Cl(-) cotransporters (KCCs) play a fundamental role

86 ires a Cl(-) gradient established in part by K(+)-Cl(-) cotransporters (KCCs).

87 The K+-Cl- cotransporters (KCCs) belong to the gene family o

88 Cl(-) exit via K-Cl cotransporters (KCCs) is a major determinant of [Cl

89 -sensitive K channel, and the electroneutral K:Cl cotransporter-leading to sickle cell dehydration.

90 s, particularly R(+)-butylindazone-sensitive K-Cl cotransporters, may have a synapse- and/or cell typ

91 In contrast, the Na(+)-K(+)-Cl(-) cotransporter Ncc69, which normally allows ch

92 tes this variation, presumably via an Na(+), K(+), Cl(-) cotransporter (NKCC) and the Shaw K(+) chann

93 mbrane conductance regulator (CFTR) or Na(+)-K(+)-Cl(-) cotransporters (NKCC) blocked alveolar fluid

94 tanide and furosemide, two blockers of Na(+)/K(+)/Cl(-) cotransporters (NKCC), inhibited all capacita

95 25% identity) to the bumetanide-sensitive Na-K-Cl cotransporter (NKCC or BSC) and the thiazide-sensit

96 The Na-K-Cl cotransporter (NKCC) mediates the coupled movement

97 The Na-K-Cl cotransporter (NKCC) plays a key role in electrolyt

98 The Na-K-Cl cotransporter (NKCC) plays central roles in cellula

99 at normally extrudes chloride and for the Na-K-Cl cotransporter (NKCC) that normally accumulates chlo

100 The human and shark Na-K-Cl cotransporters (NKCC), although 74% identical in am

101 The Na-K-Cl cotransporter NKCC1 is activated by phosphorylation

102 The secretory Na-K-Cl cotransporter NKCC1 is activated by secretagogues t

103 um preparation, AqF026 did not affect the Na-K-Cl cotransporter NKCC1.

104 osphorylation-dependent activation of the Na-K-Cl cotransporter (NKCC1) has been previously well docu

105 In particular, activation of the Na-K-Cl cotransporter (NKCC1) has been shown to be involved

106 The Na-K-Cl cotransporter (NKCC1) is expressed in most vertebra

107 re we report that a substrate of PP1, the Na-K-Cl cotransporter (NKCC1), bears this motif in its N te

108 at kidney, the "secretory" isoform of the Na-K-Cl cotransporter, NKCC1 (BSC-2), localizes to the baso

109 examine the structure and function of the Na-K-Cl cotransporter, NKCC1, we tagged the transporter wit

110 Three splice variants of the renal Na-K-Cl cotransporter (NKCC2 F, A, and B) are spatially dis

111 ternatively spliced variants of the renal Na-K-Cl cotransporter (NKCC2) are found in distinct regions

112 The renal Na-K-Cl cotransporter (NKCC2) is selectively expressed in t

113 Na-K-Cl cotransporter (NKCC2)-mediated sodium chloride reab

114 esence of furosemide, an inhibitor of the Na,K,Cl-cotransporter, NMDA-induced edema were reduced by 6

115 ID cell formation, as did inhibitors of the K:Cl cotransporter, okadaic acid, and [(dihydroindenyl)

116 KCC2, a neuronal-specific K-Cl cotransporter, plays a major role in maintaining in

117 er BSC2, one of the two major isoforms of Na-K-Cl cotransporters present in mammalian cells, can be d

118 hat a time-dependent expression of the Na(+)-K(+)-Cl(-) cotransporter protein occurs not only in cort

119 vealed that an intense staining of the Na(+)-K(+)-Cl(-) cotransporter protein was observed in dendrit

120 Na-K-Cl cotransporter protein was detected on immunoblots o

121 dient by ion substitution or by blocking the K-Cl cotransporter resulted in the starburst cells respo

122 use line expressing an activated form of the K-Cl cotransporter Slc12a4 (Kcc1), which results in a se

123 The neuron-specific K(+)-Cl(-) cotransporter SLC12A5, also known as KCC2, he

124 turally similar shark and human secretory Na-K-Cl cotransporters (sNKCC1 and hNKCC1).

125 al membrane transport systems, including the K+-Cl- cotransporter (termed KCC), cause HbS-containing

126 KCC2 is a neuron-specific K(+)-Cl(-) cotransporter that is essential for Cl(-) hom

127 ch is mediated primarily by KCC2, a neuronal K+/Cl- cotransporter that determines the intracellular c

128 ility and the activities of the Na-K-2Cl and K-Cl cotransporters, the Na/H exchanger, and the Gardos

129 Localization of the Na-K-Cl cotransporter to the plasma membrane on the blood s

130 The structure of the Na-K-Cl cotransporter transport protein is not known, but f

131 double staining demonstrates that the Na(+)-K(+)-Cl(-) cotransporter was expressed in astrocytes wit

132 lyculin A, consistent with mediation via the K+-Cl- cotransporter, was revealed by depleting deoxygen

133 erogeneous; some cells that expressed the Na-K-Cl cotransporter were weakly stained with the anti-ROM

134 We report here that kcc functions as a K(+)-Cl(-) cotransporter when expressed heterologously i

135 ctivity modulated the mRNA levels of KCC2, a K(+)-Cl(-) cotransporter whose expression correlates wit

136 de the primary link coupling activity of the K+-Cl- cotransporter with O2 tension.

137 hat the KCC1 cDNAs encode a widely expressed K-Cl cotransporter with the characteristics of the K-Cl