ライフサイエンスコーパス: K. lactis

1 K. lactis sir2 and sir4 mutant strains showed partial de

2 K. lactis sir2 mutants are more sensitive than the wild

3 K. lactis telomerase RNA, encoded by the TER1 gene, is a

4 varying from 48.3% (B. circulans) to 34.9% (K. lactis), and 19.5% (A. oryzae).

5 DNA are commonly made by recombination in a K. lactis mutant with long telomeres.

6 iring: unlike S. cerevisiae and C. albicans, K. lactis integrates nutritional signals, by means of Rm

7 than the wild type to ethidium bromide, and K. lactis sir4 mutants are more resistant phenotypes tha

8 ted in two budding yeasts, S. cerevisiae and K. lactis, have shown recombination can replenish termin

9 base triples, the 3D shape of the human and K. lactis TER pseudoknots are remarkably similar.

10 f the COX2 and COX3 mRNAs of S. kluyveri and K. lactis have little similarity to each other or to tho

11 controlling the amount of Y. lipolytica and K. lactis during production offers potential to manipula

12 Models of Y. lipolytica and K. lactis, with Penicillium roqueforti, were analysed us

13 the template and the precision of copying by K. lactis telomerase to examine primer elongation within

14 ctosidases, and at 95% lactose depletion for K. lactis beta-galactosidase.

15 tified and analyzed telomerase activity from K. lactis whole-cell extracts.

16 Thus, Sir proteins from K. lactis have roles in both silencing and telomere leng

17 A functional homolog of SIR4 from K. lactis complements the silencing defect of sir4 null

18 tity with the corresponding transporter from K. lactis but showed 53% amino acid sequence identity to

19 sport of UDP-GlcNAc into Golgi vesicles from K. lactis.

20 age of lactose hydrolysis by the immobilized K. lactis beta-galactosidase using genipin as a crosslin

21 e Mig1 revealed short patches of homology in K. lactis and K. marxianus Mig1 that might be Msn5-inter

22 The various phenotypes of sir mutants in K. lactis and S. cerevisiae, however, revealed unanticip

23 this model, we demonstrate here that RTE in K. lactis occurs by amplification of a sequence originat

24 he production of the uniform repeats seen in K. lactis.

25 We propose that short telomeres in K. lactis are not fully competent at capping chromosome

26 e by up to 10(3) near shortened telomeres in K. lactis cells.

27 Here we show that in K. lactis, truncating the Rap1p C-terminal tail (Rap1p-D

28 em arrays at telomeres when transformed into K. lactis cells.

29 In a relatively recent ancestor of K. lactis, a reorganization occurred.

30 Increasing the inoculum concentration of K. lactis resulted in decreased variation between replic

31 models inoculated with low concentrations of K. lactis exhibited blue cheese-related attributes, asso

32 cent-activated cell sorter after labeling of K. lactis cells with fluorescein isothiocyanate (FITC) c

33 Using a carboxy-terminal-tail mutant of K. lactis RAP1, we also show that, unexpectedly, RAP1 in

34 A mutant of K. lactis, mnn2-2, that lacks terminal N-acetylglucosami

35 encodes the Golgi UDP-GlcNAc transporter of K. lactis.

36 und by DNA-blot hybridization to S. pombe or K. lactis genomic DNA, and no antigenically related prot

37 appeared, and we infer that each of the six K. lactis chromosomes became circularized.

38 Under all in vitro conditions tested, K. lactis telomerase catalyzed only one round of repeat

39 Our data reported here demonstrate that K. lactis can, in at least some circumstances, make telo

40 We demonstrate that K. lactis telomerase polymerizes along the template in a

41 We further demonstrate that K. lactis telomeric fragments produce banded patterns wi

42 This indicates that K. lactis telomeres have preferred termination points wi

43 We have now cloned the gene encoding the K. lactis Golgi membrane N-acetylglucosaminyltransferase

44 We have now cloned the gene encoding the K. lactis Golgi membrane UDP-GlcNAc transporter by compl

45 kluyveri genome and deleted PET111 from the K. lactis genome.

46 tants defective in telomere maintenance, the K. lactis telomere fusions retained their telomeric DNA

47 of the 5-nt repeats defining the ends of the K. lactis telomerase RNA template in telomerase transloc

48 formed with a genomic library from wild-type K. lactis in a pKD1-derived vector; transformants were i

49 1% (v/v), the maximum GOS concentration with K. lactis beta-galactosidase was achieved in 1 and 5h at

50 ating type a (MATa) to MATalpha in the yeast K. lactis.