ライフサイエンスコーパス: KCC1

1 KCC1 encodes a 1085-residue polypeptide with substantial

2 KCC1 was identified by searching the human expressed seq

3 KCC1-specific antibody identified a 155-kDa protein in r

4 ion-polymerase chain reaction revealed a 2:1 KCC1/KCC3 mRNA ratio in VSMCs.

5 buted to potassium chloride cotransporter 1 (KCC1).

6 Although KCC1 has not been cloned from the colon, we established,

7 increase of colonic KCC1 mRNA abundance and KCC1 protein expression in potassium depletion of the ra

8 rythroblasts, a similar pattern for KCC3 and KCC1 expression during in vivo differentiation was obser

9 hreonine kinases, colocalizes with NKCC1 and KCC1/2 in diverse Cl(-)-transporting epithelia and in ne

10 inomycin D and cycloheximide increased basal KCC1 mRNA in an additive manner, suggesting different me

11 including AE3, AQP4, AQP5, CFTR, ClC2gamma, KCC1, NHE1, NKAalpha1, NKAbeta1, NKAbeta2, NKAbeta3, and

12 The increase of colonic KCC1 mRNA abundance and KCC1 protein expression in potas

13 C1 and Cl(-) efflux via K-Cl cotransporters, KCC1 or KCC2.

14 Message levels for KCC1 and KCC3b were low.

15 Human KCC1 (hKCC1) was obtained from I.M.A.G.E. clones and in

16 ibited dominant negative inhibition of human KCC1 and KCC3 and, with lower potency, mouse KCC4 and ra

17 understanding of the regulation of the human KCC1 gene, we mapped the 5' end of the KCC1 cDNA, cloned

18 ime- and concentration-dependent increase in KCC1 mRNA levels after treatment with sodium nitroprussi

19 sodium depletion resulted in an increase in KCC1 protein expression in basolateral membrane.

20 he NO/cGMP-signaling pathway participates in KCC1 mRNA regulation at the post-transcriptional level.

21 Moreover, 8-Br-cGMP increased KCC1 mRNA expression in a concentration- and time-depend

22 dely distributed K-Cl cotransporter isoform (KCC1) identified in rat brain and rabbit kidney but only

23 that VSMCs express mainly two mRNA isoforms, KCC1 and KCC3, and suggest that PKG participates post-tr

24 ast two mRNAs for K-Cl cotransporters (KCC): KCC1 and KCC3.

25 rom the rat distal colon using rabbit kidney KCC1 cDNA as a probe, the presence of an expected size m

26 membrane H,K-ATPase and basolateral membrane KCC1 protein.

27 and C-terminal cytoplasmic domains of mouse KCC1 to its K-Cl cotransport function expressed in Xenop

28 Expression of KCC mRNAs (KCC1-KCC4) in rat vascular smooth muscle cells (VSMCs) i

29 were 100% conserved between human and murine KCC1 genes.

30 otransporters KCC3, NKCC1, and NKCC2 but not KCC1 and KCC4.

31 Hydropathy analysis of KCC1 indicates structural homology to NKCC, including 12

32 l as confirming the widespread expression of KCC1 and KCC2 throughout the brain, we now show a more r

33 in erythrocytes, with variable expression of KCC1 and KCC4 among individuals that could result in mod

34 /cGMP signaling pathway in the expression of KCC1 mRNA, considered to be the major cell volume regula

35 We have reported the presence of KCC1 and KCC3 mRNAs in primary cultures of VSMCs by spec

36 sGC-dependent regulation of KCC1 mRNA expression was confirmed using YC-1, a NO-inde

37 expression of the K-Cl cotransport protein, KCC1.

38 Rabbit KCC1 (rbKCC1) and rat KCC1 (rtKCC1) were cloned by scree

39 unctional comparison of mouse KCC3 to rabbit KCC1 indicates that KCC3 has a much greater volume sensi

40 Rabbit KCC1 (rbKCC1) and rat KCC1 (rtKCC1) were cloned by screening rabbit kidney and

41 tern blot analysis of RBC membranes revealed KCC1, KCC3, and KCC4 proteins in mouse and human cells,

42 tase-polymerase chain reaction revealed that KCC1 was present in rat primary astrocytes and rat C6 gl

43 The KCC1 promoter was transactivated by forced expression of

44 is elevated in sickle erythrocytes, and the KCC1 gene has been proposed as a modifier gene in sickle

45 etanide and ethacrynic acid, which block the KCC1 potassium-chloride transporter in the kidney loop o

46 orresponding genomic DNA, and identified the KCC1 gene promoter.

47 human KCC1 gene, we mapped the 5' end of the KCC1 cDNA, cloned the corresponding genomic DNA, and ide

48 These data demonstrate that the KCC1 cDNAs encode a widely expressed K-Cl cotransporter

49 This KCC1 mRNA is substantially increased by potassium deplet

50 CC1, and that it inhibits Cl(-) exit through KCC1 and KCC2; kinase-inactive WNK3 has the opposite eff

51 to the previously characterized transporters KCC1 and KCC2, with which they share a predicted membran

52 d its co-expression did not reduce wild type KCC1 at the oocyte surface.

53 117 was co-immunoprecipitated with wild type KCC1 polypeptide, and its co-expression did not reduce w

54 basolateral membrane of duct cells, whereas KCC1, IK(Ca)1, CFTR, and ClC3 are apically localized.

55 CC3a RNA was expressed consistently, whereas KCC1 and KCC3b levels declined, and KCC4 message first i