ライフサイエンスコーパス: KiSS-1

1 ssor role in bladder cancer was revealed for KiSS-1.

2 lating MMP-9 synthesis are not the target of KiSS-1.

3 ucture, and refined chromosomal location for KiSS-1.

4 Invasion and motility were unaffected by KiSS-1.

5 Genes affected by WNT5A include KISS-1, a metastasis suppressor, and CD44, involved in t

6 ibed role in melanoma, our results show that KiSS-1 also functions as a metastasis suppressor gene in

7 ybridization histochemistry indicated robust KiSS-1 and GPR54 mRNA expression in the region of the ar

8 rous tissue results in rescued expression of KiSS-1 and reduced metastatic phenotype.

9 Sp1 are strong transcriptional regulators of KiSS-1 and that loss or decreased expression of AP-2alph

10 by AP-1, Sp1, and Ets transcription factors, KiSS-1 did not alter the binding of these factors to the

11 Thus, KiSS-1 diminishes MMP-9 expression by effecting reduced

12 did not observe any significant differential KiSS-1 expression along cell cycle by sorting analysis.

13 for the loss of tumor metastasis suppressor KiSS-1 expression and thus increased cancer metastasis.

14 ll lines, HT-1080, stably transfected with a KiSS-1 expression construct, demonstrated substantially

15 Here we show that the loss of KiSS-1 expression in highly metastatic breast cancer cel

16 human chromosome 6q16.3-q23 is essential for KiSS-1 expression in normal tissues.

17 Furthermore, we demonstrate that KiSS-1 expression is regulated by Sp1 elements within th

18 KiSS-1 expression ratios provided prognostic information

19 Patients with lower KiSS-1 expression showed a direct correlation with overa

20 ression of Sp1 and DRIP-130 not only rescues KiSS-1 expression, but also induces an inhibition of the

21 creased MMP-9 amounts was not antagonized by KiSS-1 expression, suggesting that MAPK pathways modulat

22 RIP-130-modulated transcriptional control of KiSS-1 expression.

23 xpression of this collagenase was reduced by KiSS-1 expression.

24 The expression of KiSS-1 gene and peptide and the distribution of metastin

25 KiSS-1 gene and peptide expression was higher in the hyp

26 The product of the KiSS-1 gene is absent or expressed at low level in metas

27 e undertook a study to determine whether the KiSS-1 gene, previously shown to suppress cancer spread

28 KiSS-1 has been shown to function as a tumor metastasis

29 Loss of the metastasis suppressor gene, KiSS-1 has been strongly correlated to the progression o

30 The identification and sequence of KiSS-1 (HGMW-approved symbol, KISS1), a human malignant

31 We observed complete loss of KiSS-1 in all invasive tumors under study as compared to

32 y activate the transcriptional regulation of KiSS-1 in breast cancer cells.

33 and we describe the expression of GPR54 and KiSS-1 in the hypothalamus during the peripubertal perio

34 The mechanism through which KiSS-1 is lost during metastasis, however, is still not

35 e gene product of metastasis suppressor gene KiSS-1, is the endogenous ligand for the G-protein-coupl

36 noma cells, similar to the overexpression of KiSS-1 metastasis suppressor gene in those cells.

37 KiSS-1 mRNA levels detected by real-time PCR increased w

38 es positive for MMP-9 mRNA were deficient in KiSS-1 mRNA.

39 The expression of KiSS-1 or KiSS1, like other tumor suppressor, is commonl

40 A polypeptide derived from the KiSS-1 product, designated kisspeptin-10 (Kp-10), activa

41 an chromosome 6q16.3-q23, results in reduced KiSS-1 promoter activation in highly malignant melanoma

42 domain, AP-2B, together with Sp1, increased KiSS-1 promoter activity dramatically, suggesting that A

43 ements within the first 100-bp region of the KiSS-1 promoter and that targeted deletion of a single G

44 Although the KiSS-1 promoter contains multiple AP-2alpha binding elem

45 y metastatic breast cell lines did not alter KiSS-1 promoter-driven luciferase gene activity.

46 ption does not require direct binding to the KiSS-1 promoter.

47 Based on the predicted structure of the KiSS-1 protein, our results imply a mechanism whereby Ki

48 Expression of KiSS-1 reduced metastatic potential by 95% compared to c

49 otein, our results imply a mechanism whereby KiSS-1 regulates events downstream of cell-matrix adhesi

50 Metastin, also known as KiSS-1, the cognate ligand for the metastin receptor GPR

51 suggest that DRIP-130 is a key regulator in KiSS-1 transactivation in normal tissue, and that the lo

52 lly, suggesting that AP-2alpha regulation of KiSS-1 transcription does not require direct binding to

53 p1-binding sites of the promoter to activate KiSS-1 transcription.

54 The expression pattern of KiSS-1 transcripts was analyzed using in situ hybridizat

55 Parental, vector-only transfectants and KiSS-1 transfectant clones were injected into the mammar

56 racellular matrix components was unaffected, KiSS-1 transfectants spread on immobilized type-IV colla

57 d IkappaBalpha levels in the cytosols of the KiSS-1 transfectants.

58 The expression of KiSS-1 was found to be significantly associated with his

59 Lower expression of KiSS-1 was revealed in cells derived from the most advan

60 ancer cell lines, lower transcript levels of KiSS-1 were observed in the invasive bladder carcinomas

61 d human melanoma metastasis suppressor gene, KiSS-1, which maps to chromosome 1q32-q41, could suppres