ライフサイエンスコーパス: L chain

1 uced affinity compared with the other lambda L chains).

2 n RV144 V2 Ab but paired with a mouse lambda L chain.

3 myosin L chain kinase and regulatory myosin L chain.

4 ound B cells that coexpress kappa and lambda L chain.

5 expressing the 56R H chain with the lambdaX L chain.

6 ells that express lambdaX along with another L chain.

7 chain may be compensated by mutations on the L chain.

8 e junction of the variable (Vlambdax) editor L chain.

9 through the expression of a second innocuous L chain.

10 ferritin degraded at a faster rate than the L-chain.

11 romatin when they express certain endogenous L chains.

12 ambda and some even bear two different kappa L chains.

13 unit iron-storage complex assembled of H and L chains.

14 ave inactivated the H chain and secrete only L chains.

15 he correct pairing of the two different free L chains.

16 ecific human mAb with naturally paired H and L chains.

17 tive iron core nucleation site in vertebrate L chains.

18 ct HCs that will associate mainly with kappa L chains.

19 g protein specific for a V region site on Ig L chains.

20 gement, but not selection of Iglambda editor L chains.

21 pressed primarily in combination with editor L chains.

22 ene selection differences were also noted in L chains.

23 ed as monomeric polypeptides associated with L chains.

24 s been identified as neurofilament light (NF-L) chain.

25 custom-made siRNA for canine ferritin H- and L-chains.

26 g variable region of Ig heavy (H) and light (L) chains.

27 imately 11 kDa larger (30 kDa) than standard L-chain (19 kDa) purified from canine liver.

28 nction of the microtubule-associated protein L chain 3 (LC3) conjugation system in T lymphocytes rema

29 somes using a microtubule-associated protein L chain 3 (LC3) fusion protein effectively enhances and

30 L chain 3 (LC3)-associated phagocytosis is a process in

31 possible nucleation site is observed at the L chain 3-fold pore.

32 profile that included induction of ferritin L chain, a component of the inflammatory response.

33 The overexpressed L-chain accumulated in the cytosol as predominantly homo

34 hrough expression of an additional Ig light (L) chain, accumulating gradually in lymphoid organs.

35 L chain allelic inclusion has been proposed as a B cell

36 B cells expressing two different Ig kappa L chains (allotype included) have been occasionally obse

37 However, exceptional editor L chains allow B cells to reach splenic compartments eve

38 The human H chains expressed with these L chains also have relatively high arginine (Arg) conten

39 hat expressed a transgene (Tg)-encoded kappa L chain and BM from TCR Tg mice in which the CD4 T cells

40 mutants that were impaired in assembly with L chain and in ability to be secreted from the cell.

41 old age, B2 pathways have limited surrogate L chain and increasingly generate new B cells with alter

42 iciency results in reduced usage of Iglambda L chains and a corresponding inhibition of Iglambda gene

43 d of two differently sized H chains bound to L chains and apparently often noncovalently associated w

44 were found to express both kappa and lambda L chains and display the high-affinity IgE Fc (Fcepsilon

45 tein sequences of mouse editors to all human L chains and found several human L chains similar to mou

46 Our findings can be extrapolated to human L chains and have implications for understanding a laten

47 The symmetrical arrangement of H and L chains and the disulfide crosslinks reflect adaptation

48 CX peak mAb isoform contained two unmodified L chains and two H chains terminating in glycine.

49 morphism of human and canine lens fiber cell L-chains and human H-chains.

50 e (Env) reactivity due to editing of the 2F5 L chain, and the majority of rescued B cells retained an

51 that there is no dependency on the surrogate L chain, and thus the authentic IgL proteins may be used

52 selecting VDJs that pair well with surrogate L chain, and whose maturation appears relatively indepen

53 Amplified heavy (H)- and light (L)-chain antibody segments were sequenced and used to id

54 sia ni, which reveals equal numbers of H and L chains arranged with tetrahedral symmetry.

55 ntermediate product of dioxygen reduction in L-chain as well as in H-chain and H-chain variant ferrit

56 Ferritin L chain augments CD3-induced proliferation of T cells.

57 h is removed to the media unless there is an L-chain available to form heteropolymeric ferritin.

58 Likewise, in the BALB/c.56R mouse, dual L chain B cells are found in the follicular B cell compa

59 The Jkappa usage in dual kappa L chain B cells suggests increased receptor editing acti

60 g pattern, which does not associate with the L chain (beta(2)-microglobulin).

61 competition and species origin of the MHC-I L chain (beta2-microglobulin).

62 Co-transfection of cells with both H- and L-chain cDNAs increased the intracellular levels of H-ch

63 The "escaped" dual-L-chain cells and the "ignored" low-affinity cells are t

64 These H- and L-chain characteristics were not identified in other B-C

65 This study of a large family of kappa L chain clusters in nurse shark completes the characteri

66 Only one H/L chain combination bound neutral PL and none bound dsDN

67 Eleven of 14 H/L chain combinations displayed weak binding to OVA with

68 Binding of the expressed H/L chain combinations to a range of anionic, neutral, and

69 "double-producers," suggesting that H chain/L chain combinations with superior signaling properties

70 gions of the heavy (V(H)) and kappa-light (V(L)) chain complementary DNA (cDNA) of anti-GD2 IgM hybri

71 recursor B cell receptors with the surrogate L chain complex that promotes allelic exclusion but not

72 f Ig mu heavy chains (HCs) and the surrogate L chain components lambda5 and VpreB are critical for B

73 These L-chain-containing inclusion bodies were found in the cy

74 ls, we show that secretion-competent lambda1 L chains could associate with both full-length H chains

75 d Dmu, whereas secretion-incompetent lambda1 L chains could only do so with full-length H chains.

76 ng and analyzing two comprehensive murine Ig L chain databases, one consisting of 264 monoclonal ANAs

77 rtant, although not essential, for surrogate L chain-dependent receptor signaling in primary cells, a

78 The ferritin L-chain detected in both lens types was modified and was

79 These L chains diminish or veto anti-DNA binding when expresse

80 isted in the ancestral Ig gene, before H and L chain divergence.

81 ndrome, which is caused by overexpression of L-chain due to mutation in the regulatory element in the

82 L chain-edited transgenic B cells were detectable in spl

83 Although TLR9 is dispensable for L chain editing during B cell development in the bone ma

84 sbb2(a) does not alter L chain editing frequencies of DNA Abs in the 3H9H/56R H

85 We conclude that L chain editing in anti-DNA-transgenic B cells is not on

86 Finally, we also report evidence for ongoing L chain editing in sIgM(low) transitional splenic B cell

87 otypic analysis reveals B cell depletion and L chain editing in Tg mice.

88 ding an autoreactive Ab, we reported ongoing L chain editing not only in bone marrow cells with a pre

89 gers recombination-activating gene activity, L chain editing, and deletion.

90 eted) H chain locus and evidence of vigorous L chain editing; Abs isolated from B6.Sle2(z).56R spleen

91 to SCID mice resulted in the appearance of a L chain editor (Vlambdax) in the serum of engrafted reci

92 In this study, we show that the NFkappa L chain enhancer of activated B cells 2 (NFkappaB2)/p100

93 point to possible involvement of cytoplasmic L-chain-enriched ferritin aggregates in the formation of

94 r of the L-chain resulted in accumulation of L-chain-enriched ferritin associated with cytoplasmic in

95 ed canonical, expressing a particular Vkappa L chain, evade central tolerance by down-regulating BCR

96 We ask whether a similar set of L chains exists in the human repertoire, and if so, do t

97 al role in promoting the formation of lambda L chain-expressing B cells.

98 IL and BLyS partially colocalized with kappa L chain-expressing B-lineage cells at the epithelial-con

99 We hypothesize that reduced surrogate L chain expression contributes to decreased pre-B cells

100 requency, suggesting VprBP does not regulate L chain expression from a productively rearranged Igk al

101 he timing of BBB permeability changes, kappa-L chain expression in CNS tissues, and Ab production in

102 levated CD19 levels were paralleled by kappa-L chain expression levels.

103 ation of the anti-DNA BCR caused by the dual L chain expression may prevent activation of this kappa/

104 :mCherry(+) cells and the timing of Ig H and L chain expression revealed simultaneous expression of b

105 , higher levels of CD43, and decreased kappa L chain expression.

106 erate new B cells with altered phenotype and L chain expression.

107 correlates with Ig lambda (Iglambda) light (L) chain expression and likely plays a role in receptor

108 We show that L chain ferritin is undetectable in primary fibroblasts

109 dase site mutant 222 (H62K + H65G) and human L-chain ferritin (HuLF) lack this activity.

110 Human L-chain ferritin and H-chain variants lacking functional

111 o types, ferritin H chain (FHC) and ferritin L chain (FLC).

112 HspB5 also precipitated Ig heavy and L chains from sera from patients with MS.

113 ligand-binding domain, the I domain of alpha(L) chain, from an inactive, low-affinity closed form (LA

114 nd in the B cell subpopulations, an order of L chain gene activation is suggested as: sigma-2 followe

115 , autoreactive B cells may undergo secondary L chain gene rearrangement (receptor editing) and change

116 nation of IL-7 signals and the initiation of L chain gene rearrangement remains to be elucidated.

117 e responsible for allelic exclusion, induced L chain gene rearrangement, and continued proliferation.

118 which leads to G1 arrest and induction of Ig L chain gene rearrangement, respectively.

119 (change) their specificity by secondary H or L chain gene rearrangement.

120 lacement for a functional V kappa 8J kappa 5 L chain gene.

121 and in two classes of mutations in the human L-chain gene, resulting in hereditary hyperferritinemia

122 , used a broad L chain repertoire, including L chains generating high-affinity autoreactivity, and pr

123 To understand the derivation of these L chain genes and their organizations, we performed phyl

124 uring B lymphocyte development, Ig heavy and L chain genes are assembled by V(D)J recombination.

125 Ig H chain (V(H)) and variable region kappa L chain genes expressed by six insulin binding mAb show

126 tation events are very similar at both H and L chain genes in this early vertebrate.

127 nd to be highly diverse, with the profile of L chain genes isolated from whole pancreas differing fro

128 Stage-specific rearrangement of Ig H and L chain genes poses an enigma because both processes use

129 Analysis of BCR L chain genes was used to investigate selection of B lym

130 Graves' disease, which shared the same H and L chain germline gene rearrangements and then diversifie

131 tion between the V gene segments of Ig H and L chains has been noted previously by several investigat

132 Overexpression of the L-chain has been associated with the formation of premat

133 ntain varying ratios of heavy (H) and light (L) chains; however, known ferritin structures include on

134 ceptor in dual receptor B cells is an editor L chain, i.e., neutralizes or alters self-reactivity of

135 copies of the unrearranged human H chain and L chain Ig gene loci.

136 , designated SMI, expressing unmutated H and L chain Ig genes encoding a low-affinity, polyreactive h

137 an repress the expression of a transgenic Ig L chain (IgL) by doxycycline (IgL-repressible mouse).

138 GMDV (motif-1), almost invariably express Ig L chains (IgL) encoded by IGLV3-21, whereas CLL that use

139 cteristic for MZ B cells was altered and the L chain Iglambda(+) repertoire was reduced in bumble mic

140 5 H clones were expressed with wild-type T15 L chain in vitro.

141 ges in the relative orientation of the H and L chains in both the variable and constant domains.

142 the VHH side, which normally associates with L chains in conventional antibodies.

143 ely results, in part, from reduced surrogate L chains in senescent B cell precursors and compromised

144 heightened expression of endogenous Ig H and L chains in splenic B cells, upregulation of RAG, and se

145 This model supports a role for the L-chain in iron mineralization and helps to explain the

146 caused significant accumulation of ferritin L-chain in the cells.

147 Ferritin H- and L-chains in canine and human fiber cells of healthy lens

148 ransfected with cDNAs for dog ferritin H- or L-chains in order to study differential expression of th

149 expressed the Ig variable regions of several L chain-included B cells in cell culture.

150 We find that although the number of L chain-included B cells increases as a result of recept

151 V(H)3H9 yields an anti-DNA Ab with most L chains including an anti-ssDNA with the V(kappa)8 Tg a

152 ing autoreactivity are especially subject to L chain inclusion.

153 ar adhesion molecule 1 (ICAM-1) by its alpha(L)-chain inserted domain (I-domain).

154 sulted in human VH pairing with mouse lambda L chains instead of allowing otherwise subdominant V2-gl

155 The H/L-chain interface includes complementary features respon

156 How surrogate and conventional L chains interpret Dmu's unusual structure and how that

157 uld not form receptors with a panel of kappa L chains irrespective of their secretion properties.

158 glycolipid-mCD1d complex, although only the L chain is involved in contacts with the glycolipid anti

159 ppa genes are consumed, and 3) the surrogate L chain is necessary for selection of productive IgH gen

160 k of competing cis interactions, 2) tetramer L chain is of mouse origin, and 3) Ly49 is expressed in

161 (H) chain immunoglobulin (Ig) without light (L) chain is prevented by chaperone association of the H

162 licated in the interaction between the H and L chains, is often intrinsically disordered, and is invo

163 In conclusion, a shark B cell expresses one L chain isotype at the surface and other isotypes as non

164 esults provide insight into the mechanism of L chain isotype exclusion and indicate that RS has a phy

165 genomic organization of the three zebrafish L chain isotypes and found they all differed from those

166 Two of the zebrafish L chain isotypes are encoded by two loci, each carrying

167 nes encoding one of the three nurse shark Ig L chain isotypes, called NS5.

168 two H chain isotypes, mu and omega, and four L chain isotypes, kappa, lambda, sigma, and sigma-2).

169 Nurse sharks express four L chain isotypes, kappa, lambda, sigma, and sigma-2, enc

170 for mutants from six loci of two nurse shark L chain isotypes, showing that somatic hypermutation eve

171 kappa clusters compared with the other three L chain isotypes.

172 nantly nonproductive rearrangements of other L chain isotypes.

173 Most mature B lymphocytes express one BCR L chain, kappa or lambda, but recent work has shown that

174 attached to its binding motif on the myosin L chain kinase (MLCK) promoter region, leading to the ac

175 Specifically, the role of myosin L chain kinase (MLCK) was investigated.

176 activation induced phosphorylation of myosin L chain kinase and regulatory myosin L chain.

177 The L chain lacks the residues that form a putative iron cor

178 56R/kdel mice only produce four L chains (lambda1, lambda2, lambda3, and lambdaX).

179 ymphocyte function-associated antigen 1alpha(L) chain (LFA-1alpha(L)) in these patients.

180 essible at the pro-B cell stage, whereas the L chain loci become accessible at the pre-B cell stage.

181 immunized, and V genes from the H and kappa L chain loci were sequenced.

182 ce expressing, in the physiological Ig H and L chain loci, two well-studied broadly neutralizing Abs:

183 receptor editing rearrangements at Ab H and L chain loci.

184 F4,8 may directly regulate the activation of L chain loci.

185 n SHM reporter cassette inserted into the Ig L chain locus (IgL) of chicken DT40 B cells, we have ide

186 The Ig kappa L chain locus contains multiple enhancers, including the

187 L L chain was "knocked-in" at the endogenous L chain locus.

188 to more strictly control cytosolic levels of L-chain may augment its accumulation in lenses of humans

189 s (ECs), activation of ROCK regulates myosin L chain (MLC) phosphorylation, stress fiber formation an

190 inheritance of the transgene-encoded Vkappa8 L chain, most likely a neoantigen created by the inserti

191 B6.56R mouse, polyreactive B cells with dual L chain move to the follicular B cell compartment.

192 ing indicated that non-stochastically paired L chain of IGLV3-9 contributes to the antigen binding of

193 Interestingly, the L chains of ANAs exhibited differential usage of certain

194 or the kappa (Igkappa) and lambda (Iglambda) L chains of ferret Ig.

195 r signatures, it remains unclear whether the L chains of these Abs also possess distinctive molecular

196 The expressed heavy (H) or light (L) chain of an autoreactive receptor is replaced by upst

197 HCAbs fail to incorporate light (L) chains owing to the deletion of the first constant do

198 domization of cognate heavy (H) chain/light (L) chain pairing, which could occur to a varying extent

199 transgenic H chain paired with a spectrum of L chains, predominantly recombined to J(k)1 or J(k)2.

200 A Vkappa14 L chain predominated within the complex pancreatic reper

201 Analysis of amplified Ab H and L chain products served to identify the rearranged germl

202 with decreased expression and processing of L chain protein 3 (LC3), a key component of the autophag

203 L) chain recombination and cytoplasmic kappa L chain protein expression.

204 the chains was used to alter the ferritin H:L chain ratio and to determine the effect of these chang

205 lts in the reinduction of genes required for L chain rearrangement and receptor editing.

206 nt activity at the kappa locus, and promotes L chain rearrangement and transcription.

207 y be a result not only of simultaneous H and L chain rearrangement in the shark but also of processin

208 dsDNA breaks indicative of ongoing secondary L chain rearrangement not only in bone marrow cells with

209 up-regulation of MHC class II, and augmented L chain rearrangement, resulting in a successful transit

210 ration index that may indirectly inhibit the L chain rearrangement.

211 hat alters BCR specificity through continued L chain rearrangement.

212 e clonal B cell population revealed multiple L chain rearrangements, raising the possibility of a rol

213 suggest that central tolerance and attendant L chain receptor editing affect a large fraction of norm

214 sulted in retarded clonotype development and L chain receptor editing in vivo.

215 se mice demonstrated extensive and efficient L chain receptor editing responses and had B cell number

216 ransgene, when paired with endogenous lambda L chain, recognizes the hapten 4-hydroxy-3-nitro-phenyl

217 -BCR, Igkappa germline transcription, and Ig L chain recombination.

218 J(H) recombination, and induced kappa light (L) chain recombination and cytoplasmic kappa L chain pro

219 This shift in L chain repertoire was accompanied by inhibition of deve

220 9 mice were elevated in number, used a broad L chain repertoire, including L chains generating high-a

221 only subtle effects on the glD42H-associated L chain repertoire.

222 ilar restriction characterized the preimmune L chain repertoire.

223 In contrast, slower turnover of the L-chain resulted in accumulation of L-chain-enriched fer

224 L-chain-rich ferritin in L-chain-transfected cells forme

225 Expression of a short hairpin RNA for kappa L chain RNA in B cells resulted in reduction in levels o

226 Multireactivity, allelic inclusion, and L chain secretion are three consequences of editing at t

227 Forced overexpression of free L chain secretion reinstated tumor rejection.

228 termine the degree of homology for the H and L chain sequence of individual B cells.

229 Up to 75% of the amplified H- and L-chain sequences were contained in overrepresented popu

230 with an approximately 50% framework-reverted L chain showed a 2-fold improvement in potency over the

231 iron in its Fe3+ oxidation state, while the L chain shows iron nucleation properties.

232 o all human L chains and found several human L chains similar to mouse editors.

233 Both H and L chain site-directed transgenic mice show increased B c

234 h and in frequency than any other vertebrate L chain so far reported and rivals that in H chain.

235 n the basic peak consisted of two unmodified L chain subunits and a single H chain ending in glycine,

236 FLsiRNA decreased both ferritin H- and L-chain synthesis.

237 itin H-chain synthesis, but doubled ferritin L-chain synthesis.

238 Certain L chains, termed editors, rescue anti-DNA B cells by neu

239 of mineralization more characteristic of an L-chain than an H-chain ferritin.

240 ertebrate (gnathostome) and is the essential L chain that associates with most MHC class I molecules.

241 mbinant Abs from these overrepresented H and L chains that can be used to discover the relevant Ag(s)

242 In contrast, glD42H B cells expressing L chains that confer high-affinity autoreactivity are mo

243 eased glD42H NZB/W mice is dominated by five L chains that confer no or low-affinity polyreactivity.

244 ssed the 56R H chain transgene with "editor" L chains that did not completely veto autoreactivity.

245 cells from 3H9;RS(-/-) mice were enriched in L chains that promote DNA binding.

246 Light (L) chains that edit anti-DNA heavy (H) chains rescue B-c

247 nt on the lambda5 component of the surrogate L chain, the development of B cells expressing the tg al

248 his H chain, when combined with its original L chain, the lambda1 L chain, yields a NAA that characte

249 te was present in CDR1, CDR2, or CDR3 of the L chain, the V(H) CDR2 glycan remained high mannose.

250 anti-laminin Ig in combination with multiple L chains, the H + L Ig binds ssDNA in addition to lamini

251 When combined with surrogate or conventional L chains, these V(H)14 IgH chains did not provide increa

252 -transgenic mice homozygous for the targeted L chain to exclude secondary kappa rearrangements result

253 Libraries of Vkappa L chain transcripts were generated from the spleens of c

254 L-chain-rich ferritin in L-chain-transfected cells formed inclusion bodies that w

255 round, expression of a site-directed Igkappa L chain transgene increases Igkappa(+) B cell frequency,

256 This is the second nurse shark L chain type where both germline-joined and split V-J or

257 B cells impairs selection of Igkappa editor L chains typically arising through secondary Igk rearran

258 e manner in which B cells with lambda light (L) chains undergo receptor editing, we have studied hybr

259 essing B cells and was minimally affected by L chain usage for most V(H).

260 ch, and an inverted lambda/kappa ratio of Ig L chain usage indicated that a local differentiation pro

261 gkappa and Iglambda in serum and to evaluate L chain usage of the Ab response against the hemagglutin

262 toimmunity in a polyclonal immune system, Ig L chain usage was analyzed in 3H9 site-directed IgH chai

263 HA exhibited an inherent bias toward lambda L chain usage.

264 ithin distinct PC subsets exhibited distinct L chain usage.

265 B cells and sequenced the unexpressed kappa L chains using next-generation methods.

266 ypical IgG(+) MBCs, we compared the Ab H and L chain V gene repertoires of children living in a malar

267 Second, H and L chain V gene use seems to be biased, particularly amon

268 idual 4 years before FS onset, and the H and L chain V genes of anti-DSG1 autoantibodies were analyze

269 rogation of paired H chain V region (VH) and L chain V region (VL) sequences of individual and Ag-spe

270 (BCRs) arising from the use of common H and L chain V region gene segments that share CDR3 structura

271 We sequenced Ab H and L chain V region genes (V(H) and V(L)) of clones expande

272 ific, HIV neutralizing murine IgM with H and L chain V regions (V(H) and V(L) regions) free of immuno

273 traint in the primary structure of the H and L chain V regions exist, the possibility that this level

274 We sequenced both H and L chain V(D)J rearrangements from 366 CLL patients and m

275 Germline H- and L-chain V gene Abs generated according to mutated cross-

276 ikingly similar BCRs (use of the same H- and L-chain V gene segments with unique, shared heavy chain

277 reactivity was through the use of the editor L chains, V(k)20 or V(k)21.

278 carrying functionally rearranged H chain and L chain variable region genes isolated from a B cell hyb

279 mprises associated heavy (V(H)) and light (V(L)) chain variable domains, but in camels and llamas, th

280 e and genetic origin of the endogenous kappa L chain (Vkappa or IgL) repertoire that pairs with the V

281 ecrete a monoclonal Ig containing a V region L chain (VL) epitope recognized by CD4+ T cells.

282 brane HEL in a setting in which the anti-HEL L chain was "knocked-in" at the endogenous L chain locus

283 ed sequence, while secretion of surplus free L chain was severely diminished.

284 g either human or murine beta2-microglobulin L chains was tested for all five Ly49 receptors in all f

285 Canine L-chain was approximately 11 kDa larger than standard ca

286 This excess ferritin L-chain was found in inclusion bodies, some of which wer

287 at 48 h after transfection overexpression of L-chain was much higher (9-fold over control) than that

288 than standard canine L-chain, whereas human L-chain was of the proper size.

289 Although free L chains were efficiently processed and presented by tum

290 Two predominant L chains were identified in B cells that bind heterologo

291 Vkappa L6 L chains were more promiscuous in heavy-chain usage betw

292 containing coding sequences for either H- or L-chains were used to modify ferritin subunit makeup.

293 oximately 11 kDa larger than standard canine L-chain, whereas human L-chain was of the proper size.

294 FHsiRNA blocked the degradation of ferritin L-chain, which caused significant accumulation of ferrit

295 the four positions allowed secretion of free L chain, while the combination of two secretion-restorin

296 Coexpression of secretion-restored mutant L chains with the secretion-defective mutant H chains re

297 S 120-kDa IgY comprising two equally sized H/L chains with truncated Fc and a 7S 200-kDa IgY comprise

298 e bond releasing and activating the captured L-chain with subsequent specific cleavage of the SNAP25(

299 in vertebrates comprise equally sized H and L chains, with exceptions such as H chain-only Abs in ca

300 bined with its original L chain, the lambda1 L chain, yields a NAA that characteristically binds a va