ライフサイエンスコーパス: L-FABP

1 L-FABP also increased the targeting of fluorescent LCFAs

2 L-FABP bound fluorescent VLC-PUFA with affinity and spec

3 L-FABP deletion attenuates both diet-induced hepatic ste

4 L-FABP gene ablation resulted not only in loss of L-FABP

5 L-FABP overexpression selectively increased the targetin

6 enome encodes two liver-expressed FABPs: (1) L-FABP or FABP1; and (2) Lb-FABP.

7 arkers of kidney injury (IL-18, NGAL, KIM-1, L-FABP, and albumin) and five plasma biomarkers of cardi

8 Urine NGAL, IL-18, L-FABP, and KIM-1 are sequential predictive biomarkers f

9 IL-18 and L-FABP increased at 6 h, and KIM-1 increased at 12 h.

10 At 6 h, NGAL, IL-18, and L-FABP each improved the AUC from 0.72 to 0.91, 0.84, an

11 CoASH differentially modulate the I-FABP and L-FABP dynamics, and the ligand binding sites of these p

12 y hepatocytes isolated from L-FABP (+/+) and L-FABP (-/-) mice demonstrated for the first time a phys

13 on demonstrated that higher urinary NGAL and L-FABP concentrations associated with slightly lower 6-m

14 l differentiation/maturation markers such as L-FABP, kruppel-like factor 4 (KLF4), and keratin 20.

15 in the fraction that normally comprises both L-FABP and sterol carrier protein-2 (SCP-2).

16 trast, in L35 cells the DR1 elements of both L-FABP and MTP promoters are occupied by chicken ovalbum

17 In FAO cells, the DR1 elements of both L-FABP and MTP promoters are occupied by peroxisome prol

18 Our findings show that reducing both L-FABP and MTP is an effective means to reduce VLDL secr

19 C-PUFA, correlating with its high binding by L-FABP.

20 The vesicles generated by L-FABP were sealed, contained apolipoproteins B48 and AI

21 We have therefore created L-FABP null mice and report here their initial analysis,

22 ulin but did not fuse with cis-Golgi nor did L-FABP generate COPII-dependent vesicles.

23 Gene-disrupted L-FABP mouse cytosol had 60% the activity of wild type m

24 fatty acid binding capacity, (ii) establish L-FABP as an important determinant of hepatic lipid comp

25 FAO rat hepatoma cells express L-FABP and MTP and demonstrate the ability to assemble a

26 le cell clone from FAO cells, do not express L-FABP or MTP nor do they assemble and secrete VLDL.

27 ty acid-binding proteins (FABP), liver FABP (L-FABP), and intestinal FABP (I-FABP).

28 ure, and that, in comparison to other FABPs, L-FABP may have distinctly different effects on saturate

29 intracellular lipid binding protein family, L-FABP is of particular interest as it can i), bind two

30 In addition, TFF-fed L-FABP(-/-) mice exhibited decreased hepatic fibrosis, w

31 TFF-fed L-FABP(-/-) mice exhibited reduced hepatic steatosis alo

32 tion promotes HSC activation in vivo, we fed L-FABP(-/-) and WT mice a high-fat diet supplemented wit

33 Finally, L-FABP gene ablation selectively increased the amount of

34 studies provided three new insights: First, L-FABP gene ablation reduced maximal, but not initial, u

35 Gel-filtered cytosol from L-FABP null liver lacked the main fatty acid binding pea

36 Freshly isolated HSCs from L-FABP(-/-) mice correspondingly exhibited decreased pal

37 h cultured primary hepatocytes isolated from L-FABP (+/+) and L-FABP (-/-) mice demonstrated for the

38 Primary HSCs isolated from L-FABP(-/-) mice contain fewer LDs than wild-type (WT) H

39 OFA transfer from I-FABP is faster than from L-FABP.

40 characteristics of fatty acid transfer from L-FABP are consistent with an aqueous diffusion-mediated

41 Furthermore, L-FABP expression increased the targeting of long and me

42 ggest that under fasting conditions, hepatic L-FABP contributes to hepatic LCFA oxidation and ketogen

43 Comparison of the human apo and holo L-FABP structures revealed no evidence for an "open-cap"

44 These properties of apo- and holo-L-FABP isoforms were resolved by circular dichroism, tim

45 The overall conformation of human L-FABP shows the typical beta-clam motif.

46 investigated structure and dynamics of human L-FABP with and without bound ligands by means of hetero

47 ding according to the structure of the human L-FABP/OA complex.

48 ibutes to the accumulation of cholesterol in L-FABP null liver.

49 postulate that the lipid binding process in L-FABP is associated with backbone dynamics.

50 CoA synthase mRNA was selectively reduced in L-FABP null liver.

51 ing proteins, intestinal (I-FABP) and liver (L-FABP), was examined by time-resolved fluorescence of F

52 Fatty acid-binding protein from rat liver (L-FABP) binds 2 fatty acids (FA) per protein, in contras

53 he fatty acid content of the culture medium, L-FABP expression also increased the cellular LCFA-CoA p

54 ic plots of parinaroyl-CoA binding to native L-FABP.

55 Additional studies show that ablation of L-FABP prevents hepatic steatosis caused by treating mic

56 only L-FABP and I-FABP (23% the activity of L-FABP) were active.

57 LCFA-CoA pool size, LCFA-CoA acyl chains of L-FABP (-/-) mouse livers were enriched 2.1-fold in C16:

58 erstand better the unique characteristics of L-FABP, we have carried out equilibrium binding and kine

59 antitative assessment of the contribution of L-FABP to cytosolic fatty acid binding capacity, (ii) es

60 ions suggest that the rotational dynamics of L-FABP and its conformation are more sensitive to ligand

61 To address this issue, the effect of L-FABP gene ablation on liver cytosolic LCFA-CoA binding

62 t nothing is known regarding the function of L-FABP in peroxisomal oxidation and metabolism of branch

63 -CoA binding was absent from Fraction III of L-FABP (-/-) mice.

64 theless, the soluble fraction from livers of L-FABP (-/-) mice bound 95% less radioactive oleoyl-CoA

65 primary hepatocytes isolated from livers of L-FABP gene-ablated (-/-) and wild type (+/+) mice.

66 P gene ablation resulted not only in loss of L-FABP but also in concomitant upregulation of two other

67 promoter element present in the promoters of L-FABP and MTP affects transcription, expression, and VL

68 miscuous binding and transport properties of L-FABP, we investigated structure and dynamics of human

69 d for the first time a physiological role of L-FABP in the uptake and metabolism of branched-chain fa

70 were tested for PCTV budding activity; only L-FABP and I-FABP (23% the activity of L-FABP) were acti

71 lly with increasing concentrations of BSA or L-FABP, proteins that exhibit diffusional transfer kinet

72 l CoA bound to L-FABP also reflected overall L-FABP motion but yielded longer rotational correlation

73 ransfected L-cell fibroblasts overexpressing L-FABP using a series of fluorescent fatty acids differi

74 t 10 degrees C, and that 2 FA were bound per L-FABP for all temperatures and FA.

75 ression of liver fatty-acid binding protein (L-FABP) and adipocyte fatty acid-binding protein (aP2),

76 ession for liver fatty acid-binding protein (L-FABP) and I-FABP.

77 proteins, liver fatty acid-binding protein (L-FABP) and MTP, which cooperatively shunt fatty acids i

78 ssing liver-type fatty acid-binding protein (L-FABP) by real time multiphoton laser scanning microsco

79 , and liver-type fatty acid binding protein (L-FABP) from 1304 deceased donors at organ procurement,

80 Liver fatty acid binding protein (L-FABP) has been proposed to limit the availability of l

81 he role of liver fatty acid-binding protein (L-FABP) in the uptake, transport, mitochondrial oxidatio

82 Although liver fatty acid-binding protein (L-FABP) is an important binding site for various hydroph

83 native rat liver fatty acid binding protein (L-FABP) is composed of isoforms differing in isoelectric

84 Although liver fatty acid binding protein (L-FABP) is known to bind not only long chain fatty acid

85 y that the liver fatty acid-binding protein (L-FABP) may function in this role was addressed in trans

86 , and liver-type fatty acid binding protein (L-FABP) were measured in spot urine samples and standard

87 Liver fatty acid binding protein (L-FABP), a cytosolic protein most abundant in liver, is

88 M-1), liver-type fatty acid binding protein (L-FABP), and albumin differed between etiologies and wer

89 M-1), liver-type fatty acid binding protein (L-FABP), and interleukin (IL)-18, is reviewed.

90 min (BSA), liver fatty acid-binding protein (L-FABP), and intestinal fatty acid-binding protein (I-FA

91 howed that liver fatty acid-binding protein (L-FABP; binds LCFA-CoA as well as LCFA) significantly co

92 n [IL]-18, liver fatty acid-binding protein [L-FABP], and kidney injury molecule [KIM]-1) for cardiac

93 expression of a fatty acid-binding protein, L-FABP, specifically enhanced uptake and intracellular t

94 albumin, liver fatty acid binding proteins (L-FABP), and organic anion transporters--determine the d

95 Regarding n-3 and n-6 VLC-PUFA, L-FABP expression enhanced uptake into the cell and cyto

96 ain upon ligand binding, as proposed for rat L-FABP.

97 Thus, rat L-FABP isoforms differ markedly in both structure and li

98 alpha and PGC-1beta coordinately up-regulate L-FABP and MTP expression, by competing with chicken ova

99 Second, L-FABP gene ablation inhibited phytanic acid peroxisomal

100 We conclude that L-FABP can select cargo for and bud PCTV from intestinal

101 It is hypothesized that L-FABP may act as a cytosolic buffer for fatty acids, ma

102 arinaric acid displacement assay showed that L-FABP bound BODIPY-C12 and BODIPY-C16 with K(i)s of 10.

103 CLSM and MPLSM showed that L-FABP expression enhanced by 2-4-fold the initial rate

104 -) mice demonstrated for the first time that L-FABP is a physiologically significant contributor to d

105 Third, lipid analysis of the L-FABP gene-ablated hepatocytes revealed an altered fatt

106 Thus, L-FABP may function as a carrier for selectively enhanci

107 rinaric acid and cis-parinaroyl CoA bound to L-FABP also reflected overall L-FABP motion but yielded

108 suggest that the conformation of FA bound to L-FABP may differ with both FA type and temperature, and

109 e heat capacity changes, which are unique to L-FABP, do not appear to be correlated with a significan

110 otein fraction (Fraction III) from wild-type L-FABP (+/+) mice, isolated by gel permeation chromatogr

111 % less radioactive oleoyl-CoA than wild-type L-FABP (+/+) mice.

112 nscripts in the villar tips suggests (unlike L-FABP) that older terminally differentiated cell popula

113 Urine L-FABP did not associate with any study outcomes.

114 Here, we used L-FABP null mice to test this hypothesis.

115 fluorescence photobleaching recovery, where L-FABP gene ablation reduced the cytoplasmic, but not me

116 s by a nontranscriptional mechanism, whereas L-FABP can activate ketogenic gene expression in fed mic

117 Thus, the mechanisms whereby L-FABP affects fatty acid oxidation may vary with physio

118 living cells and suggested a model, whereby L-FABP facilitated VLC-PUFA targeting to nuclei by enhan

119 To investigate whether L-FABP deletion promotes HSC activation in vivo, we fed

120 While L-FABP gene ablation did not alter liver LCFA-CoA pool s

121 In summary, these data with L-FABP (-/-) mice demonstrated for the first time that L

122 centration of FFA in the reaction of FA with L-FABP.

123 in the I-FABP ligand binding site than with L-FABP.