ライフサイエンスコーパス: L. mexicana

1 L. mexicana Ag-stimulated lymph node cell culture from t

2 strong protection in recipient mice against L. mexicana infection, suggesting that attenuated L. don

3 ma significantly enhances resistance against L. mexicana that is associated with a significant suppre

4 derivatives of 1 display IC50 values against L. mexicana GAPDH of 16 and 4 microM, respectively (3100

5 Although L. mexicana infection was associated with minimal expans

6 L. major (LV39), L. amazonensis and L. mexicana were the most efficient utilizers of leucine

7 nlike mammalian aldolases, the T. brucei and L. mexicana aldolases contain nonameric N-terminal type

8 to use the X-ray structures of T. brucei and L. mexicana GAPDHs containing bound NAD+ to design adeno

9 nge 2-12 microM for T. brucei, T. cruzi, and L. mexicana GAPDH's, and these compounds did not inhibit

10 ngs of atypical cutaneous leishmaniasis, and L. mexicana from lesion aspirates from infected hamsters

11 Sand fly gut populations of both L. mexicana and L. infantum were significantly reduced i

12 peed videomicrographs showed that T. brucei, L. mexicana and a T. brucei RNAi morphology mutant have

13 eatment of cutaneous leishmaniasis caused by L. mexicana.

14 of chronic cutaneous leishmaniasis caused by L. mexicana.

15 ort a model in which ligation of FcgammaR by L. mexicana-bound immunoglobulin G promotes IL-10 produc

16 rd a Th1-like response and control cutaneous L. mexicana infection.

17 nses and has a role in controlling cutaneous L. mexicana infection.

18 gh IL-10-deficient mice infected with either L. mexicana or L. amazonensis failed to control the lesi

19 y combining differential expression data for L. mexicana, L. major and Leptomonas seymouri, we have i

20 We have now solved the structure of ICP from L. mexicana by NMR and shown that it adopts a type of im

21 can provide protection against heterologous L. mexicana parasites by induction of a strong T cell re

22 copy cysteine protease B (CPB) gene array in L. mexicana is associated with decreased parasite virule

23 BTB 06237 also reduced parasite burdens in L. mexicana-infected J774 macrophages at low micromolar

24 cytokine production and profound defects in L. mexicana-specific Ig isotype class switching to IgG1

25 L-10 generated by IgG-FcgammaR engagement in L. mexicana infection.

26 l, and genetic dissection of ARG function in L. mexicana promastigotes establishes: (i) that the enzy

27 maRIII in suppressing protective immunity in L. mexicana infection, likely through macrophage IL-10 p

28 t a significant amount of the Ca2+ stored in L. mexicana amazonensis promastigotes and amastigotes is

29 However, lpg2(-) L. mexicana amastigotes similarly lacking PGs but otherw

30 ransfected with a cosmid expressing multiple L. mexicana CPB genes, this parasite induced a significa

31 c. inoculation with 5 x 10(6) amastigotes of L. mexicana into the shaven rump, STAT6+/+ mice develope

32 In the case of L. mexicana complex parasites (L. mexicana, L. pifanoi,

33 and elucidate its role in the cell cycle of L. mexicana.

34 ted with L. mexicana was due to a failure of L. mexicana to activate T cells.

35 oach featuring arginase-deficient mutants of L. mexicana lacking both alleles of the gene encoding ar

36 L-4 signaling is critical for progression of L. mexicana infection in genetically susceptible mice an

37 ese data indicate that cysteine proteases of L. mexicana are critical in suppressing protective immun

38 of IL-10 or FcgammaR leads to resolution of L. mexicana disease and support a model in which ligatio

39 y reported orthorhombic crystal structure of L. mexicana GAPDH (LmGAPDH): the unusual conformation of

40 Two crystal structures of L. mexicana glyceraldehyde-3-phosphate dehydrogenase in

41 Transfection of L. mexicana amazonensis cells with this recombinant plas

42 dependent upon IL-12p40, since treatment of L. mexicana-infected IL-10-/- mice with anti-IL-12p40 mo

43 Antibody-opsonized L. mexicana induced IL-10 production by B6 macrophages i

44 n the case of L. mexicana complex parasites (L. mexicana, L. pifanoi, and L. amazonensis), a critical

45 espite ample levels of T cell proliferation, L. mexicana fails to induce substantial lymph node expan

46 espects, the lpg2(-)REV amastigotes resemble L. mexicana amastigotes.

47 Furthermore, B6 FcRgamma-/- mice resolve L. mexicana lesions, and lymph node cells from these mic

48 Second, L. mexicana infection fails to drive the differentiation

49 ishmaniasis caused by the New World species, L. mexicana and L. amazonensis, we analyzed their course

50 In this study, we show that L. mexicana cysteine proteases suppress the antileishman

51 The study suggests that L. mexicana CPA and CPB perform similar roles to the asp

52 The L. mexicana GAPDH subunit exhibits substantial structura

53 associated with infection by members of the L. mexicana complex.

54 The tip of the L. mexicana flagellum was frequently intimately associat

55 signaling in the intracellular stage of the L. mexicana life cycle.

56 We now show that C3H mice infected with the L. mexicana deletion mutant (Deltacpb) initially develop

57 T cells (both CD4+ and CD8+) and heal their L. mexicana lesions, with parasite control.

58 from wild-type T. brucei (highly chiral) to L. mexicana (near-axial symmetry).

59 ow that FcgammaRIII KO mice are resistant to L. mexicana infection, resolving lesions in association

60 hermore, we found that T cells responding to L. mexicana infection were less able to differentiate in

61 tage of T cells proliferating in response to L. mexicana and L. major.

62 The initial antibody response to L. mexicana is an immunoglobulin G1 (IgG1) response, and

63 stronger IgG2a/c, IgG3, and IgM responses to L. mexicana infection and yet are more resistant to the

64 y not be the mechanism for susceptibility to L. mexicana infection, unlike for L. major infection.

65 n was secreted/released by these transfected L. mexicana parasites and that it possessed functional c

66 Upon challenge with wild-type L. mexicana, mice immunized either for short or long per

67 ow at rates comparable to those of wild-type L. mexicana-infected mice.

68 results also provide an explanation for why L. mexicana CPA/CPB-deficient mutants transform to amast

69 the minimal immune response associated with L. mexicana infection.

70 her the nonhealing phenotype associated with L. mexicana was due to a failure of L. mexicana to activ

71 tion progressed, STAT6+/+ mice infected with L. mexicana displayed significantly higher titers of Lei

72 ceptibility of BALB/c mice to infection with L. mexicana and L. amazonensis, does play a significant

73 infected FcgammaRIII knockout (KO) mice with L. mexicana.