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1 LAMP and qPCR exhibited similar analytical sensitivities
2 LAMP dramatically lowers the detection threshold achieva
3 LAMP is one of the most promising point-of-care (POC) ge
4 LAMP primers were designed to target a conserved gene en
5 LAMP-2A is a transmembrane protein component for protein
6 LAMP-2A is required for chaperone-mediated autophagy (CM
7 LAMP-2B regulates lysosome maturation to impact macroaut
8 LAMP-2C levels modulated the steady-state expression of
9 ociated with lysosomal-associated protein-1 (LAMP/gag) is a potential therapeutic vaccine against HIV
10 sosome associated membrane protein (LAMP)-1, LAMP-2 and the a2 isoform of V-ATPase (a2V, an enzyme in
11 cation using the lysosomal marker protein 2 (LAMP-2) immunolabeling showed higher neuronal lysosomal
13 the lysosome-associated membrane protein-2 (LAMP-2) regulate these pathways influencing immune recog
14 ysosome-associated membrane protein type 2a (LAMP-2A) together with a complex of chaperones and co-ch
16 amplified fields (hot spots) in and around a LAMP are at the expense of other regions with depleted f
20 via loop mediated isothermal amplification (LAMP) allowing for quantitative analysis of the cMYC pro
21 ning loop-mediated isothermal amplification (LAMP) and AC susceptometry, we demonstrate a rapid and h
22 ered loop-mediated isothermal amplification (LAMP) and real-time electrochemical detection based on t
23 ion: Loop-mediated isothermal amplification (LAMP) and Rolling Circle Amplification (RCA) techniques
24 of a loop-mediated isothermal amplification (LAMP) assay (illumigene assay) for the diagnosis of GAS
25 1&2 loop-mediated isothermal amplification (LAMP) assay (Meridian Bioscience, Cincinnati OH) for the
26 A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the gluta
28 y, a loop-mediated isothermal amplification (LAMP) assay provided rapid detection of SbGP/MPV phytopl
29 ed a loop-mediated isothermal amplification (LAMP) assay that enables rapid, low-cost detection of M.
30 of a loop-mediated isothermal amplification (LAMP) assay to facilitate rapid inexpensive molecular di
32 sign loop-mediated isothermal amplification (LAMP) assays for the rapid, specific, sensitive, and qua
33 tric loop-mediated isothermal amplification (LAMP) assays targeting the L. loa-specific gene sequence
34 for loop-mediated isothermal amplification (LAMP) assays were identified from draft genomes of Calon
36 with loop-mediated isothermal amplification (LAMP) for multiplexed instrument-free diagnosis of these
37 with loop-mediated isothermal amplification (LAMP) for the rapid, sensitive, and instrument-free dete
41 sing loop-mediated isothermal amplification (LAMP) method and subsequently added to luminol solution
43 the loop-mediated isothermal amplification (LAMP) of nucleic acid biomarkers and shows for the first
44 les, loop-mediated isothermal amplification (LAMP) of nucleic acids, and visual readout of assays.
45 the loop-mediated isothermal amplification (LAMP) reaction which results in improved performance.
46 of a loop-mediated isothermal amplification (LAMP) reaction with an initial number of DNA strands as
47 izes loop-mediated isothermal amplification (LAMP) reagents predeposited into distinct lanes of the m
48 A loop-mediated isothermal amplification (LAMP) system, the illumigene Mycoplasma DNA amplificatio
49 ined loop-mediated isothermal amplification (LAMP) technique with QCM, called as LAMP-QCM, for detect
50 n by loop-mediated isothermal amplification (LAMP) to amplify specifically the E. coli Tuf gene after
52 ming Loop-mediated isothermal amplification (LAMP) was developed for the detection and quantification
53 free loop mediated isothermal amplification (LAMP) was monitored in real-time with a point of use dev
54 ines loop-mediated isothermal amplification (LAMP) with a portable tube scanner real-time isothermal
55 rate loop-mediated isothermal amplification (LAMP) with ECL technique for DNA detection and quantific
56 and loop-mediated isothermal amplification (LAMP) with end point detection using a hand-held UV sour
58 the loop mediated isothermal amplification (LAMP), are well suited for field use as they do not requ
59 ined loop-mediated isothermal amplification (LAMP), programmable toehold-mediated strand-exchange sig
62 cally simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP p
65 the T4-bacteriophage-modified biosensor and LAMP can achieve screening, viability, and confirmation
66 n this study we identify LAMP-1 (CD107a) and LAMP-2 (CD107b) on the surface of human monocyte-derived
69 exhibited a striking accumulation of LC3 and LAMP-1 positive autolysosomes containing undigested cyto
70 of LAMP-SPR sensing was 10 copies/microl and LAMP-SPR sensing system showed a good selectivity toward
71 tion in LEs similar to that seen in NPC- and LAMP-deficient cells, we show that the restriction of pa
79 ermore, Wistar Kyoto rats injected with anti-LAMP-2 antibodies did not develop glomerulonephritis.
80 ication (LAMP) technique with QCM, called as LAMP-QCM, for detection of high-risk human papillomaviru
82 oop-mediated isothermal amplification assay (LAMP) for detection of HBV genotypes A, B, C, D, E, and
83 etection requirement, either in real-time at LAMP running temperature (~60 degrees C), or at end-poin
84 gDNA] and/or 5 mf/ml) than the LLMF342-based LAMP assay (10 pg/ml of gDNA and/or 50 mf/ml), and its a
87 l detection based on the interaction between LAMP amplicon and the redox-reactive osmium complex.
91 h 3-well nested PCR, the sensitivity of both LAMP and single-well nested PCR was 90%; the microscopy
92 immediate reporting of half of all TPs, but LAMP or CCNA was required to confirm the presence or abs
96 l killer (NK) cells and deficiency of CD107a/LAMP-1 in mice resulted in increased NK cell apoptosis u
98 hus, our data support a novel role of CD107a/LAMP-1 in the protection of NK cells from degranulation-
101 Engineered surface expression of CD107a/LAMP-1, but not of CD107b/LAMP-2, reduced the granule-me
104 pression of CD107a/LAMP-1, but not of CD107b/LAMP-2, reduced the granule-mediated killing of transfec
106 mplification capacities of TPrA and combines LAMP with inherently high sensitivity of the ECL techniq
112 ults of adding swarm primers to conventional LAMP reactions include increased amplification speed, in
116 with the presence of gene products LAMP3/DC-LAMP and CD83, which typify mature dendritic cells (DCs)
117 toplasm reactivity in a large fraction of DC-LAMP(+) cells, instead of the classical dot-like stain.
118 breast tumor samples, and the density of DC-LAMP(+) DCs clusters was strongly correlated with the de
119 ssociated membrane glycoprotein positive (DC-LAMP+)) DCs, but with marked decreases in expression of
122 e as shown by minimal colocalization with DC-LAMP, and coexpressed inflammatory markers tumor necrosi
123 a pool of candidate markers, we demonstrate LAMP assay specificity by testing related fungal species
124 hm-based strategy is then employed to design LAMPs that selectively enhance the response of analyte m
125 main lamellar morphology, which we designate LAMP Transmission electron microscopy indicates that LAM
127 sothermal amplification (qLAMP), and digital LAMP (dLAMP), for the detection and quantification of hu
128 ophilic PDMS surfaces that allow for digital LAMP to be performed in a self-driven microfluidic devic
132 count and the expected concentration) of dRT-LAMP 10-fold, from approximately 2% to approximately 23%
134 gital format that counts molecules using dRT-LAMP chemistry detected a 2-fold change in concentration
135 Consequently, deletion of the gene encoding LAMP-2A in T cells caused deficient in vivo responses to
136 more, B. neotomae exhibited early endosomal (LAMP-1) and late endoplasmic reticulum (calreticulin)-as
138 will likely benefit most if not all existing LAMP assays based on state-of-the-art, six-primer reacti
140 on of paper into the microfluidic device for LAMP reactions enables stable test results over a much l
142 antees a significant and reliable signal for LAMP reaction amplified from as little as 10(-19) M viru
143 owever, common detection techniques used for LAMP and RT-LAMP have drawbacks, including poor discrimi
145 identified two VEGF-C/NRP-2-regulated genes, LAMP-2 and WDFY-1, that have previously been suggested t
151 focus on the detection of purified DNA here, LAMP has previously been demonstrated with a range of cl
152 ynamics of the transmembrane domain of human LAMP-2A in n-dodecylphosphocholine micelles by nuclear m
153 c loop-mediated isothermal amplification (iD-LAMP) and quantitative optical read-out by a disc drive.
157 e alternating metal and dielectric shells in LAMPs are designed such that, when illuminated, the plas
167 The performance of a commercial malaria LAMP assay (Illumigene Malaria LAMP) was evaluated using
169 rcial malaria LAMP assay (Illumigene Malaria LAMP) was evaluated using two sample preparation workflo
170 Microscopy was performed, and then malaria LAMP was performed using Plasmodium genus and Plasmodium
173 colocalizes with mature phagolysosome marker LAMP-1 and with vacuolar proton ATPase in macrophages.
175 ess the diagnostic accuracy of meningococcal LAMP as a near-patient test in the emergency department.
176 s and tested with near-patient meningococcal LAMP and the results were compared with those obtained b
177 lations using Linkage Disequilibrium method (LAMP-LD) and evaluated the association with common carot
178 iparum qPCR titer of >/= 2 parasites/microL, LAMP sensitivity was 97.8% (95% confidence interval, 93.
180 tibodies from all 8 patients bound to native LAMP-2 purified from human glomeruli and recombinant hLA
182 matrices such as human blood, using a novel LAMP primer set for bacteriophage MS2 (a model RNA virus
184 om M. smegmatis decreases the association of LAMP-3 with bead phagosomes, indicating that P27 itself
188 enomics-based approach to the development of LAMP diagnostic assays is the first of its kind for fung
189 epant results resolved 4 results in favor of LAMP, suggesting that the primary analysis had underesti
196 In order to both improve the specificity of LAMP detection and to make readout simpler and more reli
201 respiratory and blood specimens with use of LAMP was accurate (sensitivity 89% [95% CI 72-96], speci
203 derived dendritic cells (MoDC) and show only LAMP-2 is internalized after ligation by specific Abs, i
205 n of Col-I with LC3, an autophagy marker, or LAMP-1, a lysosome marker, whereas treatment with TFP, a
207 limits of detection of a few DNA copies per LAMP zone for N. meningitidis, S. pneumoniae and Hib wer
208 ion of N. meningitidis is about 3 copies per LAMP zone within 45 min, close to single-bacterium detec
211 tions in a simple, inexpensive and portable "LAMP box" supplemented with a consumer class smartphone.
215 or the lysosome-associated membrane protein (LAMP) and by pharmacological inhibition of phagosome aci
216 The lysosome-associated membrane protein (LAMP) family includes the dendritic cell endocytic recep
217 and DC-lysosome-associated membrane protein (LAMP), low levels of MIP1-beta/CCL4, MIG/CXCL9, and seve
218 ent of lysosome associated membrane protein (LAMP)-1, LAMP-2 and the a2 isoform of V-ATPase (a2V, an
220 ellular Salmonella recruit the host proteins LAMP-2A and Hsc73, key components of the host protein tu
221 d the lysosome-associated membrane proteins (LAMP) 1 and 2 are important for robust liver-stage P. be
222 the lysosomal-associated membrane proteins (LAMPs) appear on the cell surface after CG exocytosis, w
224 iated isothermal DNA amplification reaction (LAMP) targeting a pathogenic bacteria gene, showing bene
228 n-loop-mediated isothermal amplification (RT-LAMP) and the ESEQuant tube scanner, a portable isotherm
229 loop-mediated, isothermal amplification (RT-LAMP) assay for rapid detection of ZIKV and its implemen
230 n-loop-mediated isothermal amplification (RT-LAMP) assay, quantitative RT-PCR (qRT-PCR), and IgM- and
231 n-loop-mediated isothermal amplification (RT-LAMP) has frequently been proposed as an enabling techno
233 n loop-mediated isothermal amplification (RT-LAMP) was used subsequently to amplify the viral RNA.
234 n loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorpo
236 transcription, exponential amplification (RT-LAMP), and linear degradation (restriction enzymes) star
237 e loop-mediated isothermal amplification (RT-LAMP), demonstrating that the plasma is sufficiently pur
239 ranscription loop-mediated amplification, RT-LAMP) in the context of fluctuations in temperature and
240 mbination with ELISA, both the RT-RPA and RT-LAMP assays increased the detection of acute DENV infect
242 on detection techniques used for LAMP and RT-LAMP have drawbacks, including poor discrimination capab
243 ction limit of the newly developed immuno-RT-LAMP assay was as low as 1.62x10(1) 50% embryo infectiou
245 mediated isothermal amplification (immuno-RT-LAMP) assay for the rapid detection of AIV H5N1 in whole
248 concordance (kappa of >/=0.723) with the RT-LAMP and qRT-PCR assays in detecting the dengue viremic
249 into the microfluidic chip to detect the RT-LAMP reaction product directly on the chip itself by mea
251 N1 gene was exponentially amplified using RT-LAMP on either a real-time PCR instrument for quantitati
258 eru to establish the feasibility of using TB-LAMP (Eiken Chemical Co.) in microscopy laboratories com
259 nsmission electron microscopy indicates that LAMP exhibits ACBC domain connectivity, in contrast to c
260 nforces these observations and predicts that LAMP is thermodynamically stable below a critical chiAC,
262 r LAMP2 and NPC1 protein levels suggest that LAMP proteins represent a significant cholesterol bindin
267 F72-based qPCR and time to positivity by the LAMP assay, and performance measures of sensitivity, spe
270 and 10 to 20 CFU/25 g) of STEC cultures, the LAMP assays achieved accurate detection after 6 to 8 h e
272 were 98.4% and 98.1%, respectively, for the LAMP P. falciparum primers and 97.0% and 99.2%, respecti
273 of the four loop products amplified from the LAMP is transduced to an active catalyst ssDNA which can
275 (9) cfu/mL, while the linear response of the LAMP approach was determined to be 10(2)-10(7) cfu/mL.
278 utive loading, mixing, and aliquoting of the LAMP primers/reagents and DNA sample solutions were acco
284 reproduced the higher mobility forms of the LAMPs, suggesting that Pompe iPSC-CMs produce LAMPs lack
287 ) -alkylguanine-DNA alkyltransferase) led to LAMP-to-hCG signal transduction on low-cost, commerciall
288 epidermal growth factor (EGF) trafficking to LAMP-2-positive compartments and EGF receptor degradatio
290 sputum and blood hemoculture were undergone LAMP amplification for 0.18 kbp and 0.23 kbp DNA fragmen
291 between genotypes and TOF was assessed using LAMP.A rare SNP (c.807C > T; rs56085230) discovered by s
293 erve fibers but was found to colocalize with LAMP-1 and cathepsin D during early stages of axonal spr
294 CpG-A shows higher co-localization with LAMP-2(+) lysosomes than CpG-B and induces DNase II loca
295 ticles (streptavidin-MNPs) are premixed with LAMP reagents including the analyte and biotinylated pri
296 full integration of sample preparation with LAMP amplification and end point detection with a limit
297 In conclusion, antibodies that react with LAMP-2 may exist at very low titers in a minority of pat
299 tion of mice with mouse DCs transfected with LAMP/gag (Lg-mDCs) stimulated more potent B- and T-cell
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