ライフサイエンスコーパス: LNA

1 LNA (locked nucleic acids, i.e. oligonucleotides with a

2 LNA finds small highly conserved network regions and pro

3 LNA modified ASOs can cause hepatotoxicity, and this ris

4 LNA was used in three positional patterns: near the 5' e

5 LNA-5' increased the average Phred Q30 score by 60% and

6 LNA-92a exerts cell-protective, proangiogenic, and anti-

7 LNA-92a significantly (P<0.01) reduced miR-92a expressio

8 LNA-anti-miR-192 significantly reduced levels of miR-192

9 LNA-antimiR-34 (8-mer) efficiently silenced all three mi

10 LNA-CTGs produced rapid and sustained improvement of mot

11 LNA-FISH requires 5 h, or between 10 and 36 h when combi

12 LNA-modified chemistries can effectively silence miR-15

13 LNA-modified TFOs form more stable triplexes than alpha-

14 LNAs targeting sequences at or near transcription start

15 The RNA with the 5'-LNA-modified cap was found to be approximately 1.61- and

16 rotein decreased activity of PS-ASOs with 5'-LNA or 5'-cEt wings, but not with 5'-MOE wing.

17 The RNA capped with the m(7(LNA))G[5']ppp[5']G 3 cap analogue was translated the mos

18 Molecular modeling of the m(7(LNA))G[5']ppp[5']G 3 cap analogue with the cap binding p

19 st that the new antireverse cap analogue m(7(LNA))G[5']ppp[5']G 3 is a potential candidate for RNA-ba

20 leic acid (LNA)-modified cap analogue 3, m(7(LNA))G[5']ppp[5']G, has been synthesized and its biologi

21 ked nucleic acid-modified antisense miR-92a (LNA-92a) was applied either regionally (antegrade or ret

22 NAs) to the beacon structure, resulting in a LNA molecular beacon (LMB) with robust stability after s

23 ulation of MOR by let-7 was revealed using a LNA-let-7 inhibitor to knockdown let-7 in SH-SY5Y cells.

24 sized from a precursor alpha-linolenic acid (LNA) or obtained preformed in the diet.

25 studies in vitro and by locked nuclear acid (LNA) modified inhibitors in vitro and in vivo.

26 ed by direct sequencing, locked nuclei acid (LNA)-based PCR, and immunohistochemistry.

27 Locked nucleic acid (LNA) and its diastereomer alpha-L-LNA are two interestin

28 ted nucleotides such as locked nucleic acid (LNA) are very popular as affinity-, specificity-, and st

29 take full advantage of locked nucleic acid (LNA) based molecular beacons (LNA-MBs) for a variety of

30 tor capped hairpin with locked nucleic acid (LNA) bases on the dynamics and efficiency of hole transp

31 First, locked nucleic acid (LNA) bases were incorporated into the probes to normaliz

32 Locked Nucleic Acid (LNA) can enhance oligonucleotide affinity and specificit

33 hococcus, Prochlorococcus, low nucleic acid (LNA) content bacterioplankton and small plastidic protis

34 fications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA cleavage have been sh

35 vivo administration of locked nucleic acid (LNA) inhibitors that suppression of endogenous miR-29 lo

36 Locked nucleic acid (LNA) microarrays were used to investigate the miRNA tran

37 oxyethylribose (MOE) or locked nucleic acid (LNA) modifications were designed to investigate whether

38 A-BCA system contains a locked nucleic acid (LNA) modified DNA probe for improving hybridization effi

39 -1-yl)carbonyl-2'-amino locked nucleic acid (LNA) monomer X is a highly versatile building block for

40 d by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands.

41 ted nucleotides such as locked nucleic acid (LNA) monomers are used extensively in molecular biology

42 fied with six different locked nucleic acid (LNA) monomers, i.e. conventional and C5-alkynyl-function

43 -methoxyethyl (MOE) and locked nucleic acid (LNA) nucleosides yielded a series of nucleoside modifica

44 e use of functionalized locked nucleic acid (LNA) oligomers as biomolecular handles that permit seque

45 RNAs in plants by using locked nucleic acid (LNA) oligonucleotide probes.

46 petitively blocked by a locked nucleic acid (LNA) oligonucleotide.

47 ates containing DNA and locked nucleic acid (LNA) oligonucleotides are active, and oligospermine conj

48 Transfection of locked nucleic acid (LNA) oligonucleotides designed to block the function of

49 ults were obtained with locked nucleic acid (LNA) phosphorothioate gap-mers.

50 also demonstrated that locked nucleic acid (LNA) probes improved sensitivity approximately 4-fold co

51 the targeted loci, and locked nucleic acid (LNA) probes were used to enhance the detection of strain

52 goxigenin (DIG) RNA and locked nucleic acid (LNA) probes, respectively.

53 Use of locked nucleic acid (LNA) residues in the DNA probes resulted in greater disc

54 Locked nucleic acid (LNA), a conformationally restricted DNA analogue, is pot

55 es comprising DNA, RNA, locked nucleic acid (LNA), or 2'O-methyl-DNA.

56 the let-7 family with a locked nucleic acid (LNA)-anti-miR has the opposite effect.

57 With the use of locked nucleic acid (LNA)-antimiR-21 oligonucleotides, bimodal imaging vector

58 Locked nucleic acid (LNA)-based miRNA array was employed to profile miRNAs us

59 h DNA, RNA and combined locked nucleic acid (LNA)-DNA backbones can all detect miRNAs of low (<1 nM)

60 iac tissues and whether locked nucleic acid (LNA)-modified anti-miR chemistries can target cardiac ex

61 of 8-mer seed-targeting locked nucleic acid (LNA)-modified anti-miR oligonucleotides, termed tiny LNA

62 ed seed-targeting 8-mer locked nucleic acid (LNA)-modified antimiR (LNA-antimiR-34) can provide thera

63 sed miRNA-122 using the locked nucleic acid (LNA)-modified antisense oligonucleotide miravirsen.

64 the present study, the locked nucleic acid (LNA)-modified cap analogue 3, m(7(LNA))G[5']ppp[5']G, ha

65 uated the efficacy of a locked nucleic acid (LNA)-modified inhibitor of miR-192, designated LNA-anti-

66 cted chimpanzees with a locked nucleic acid (LNA)-modified oligonucleotide (SPC3649) complementary to

67 n suspended cells using locked nucleic acid (LNA)-modified oligonucleotide probes.

68 d cultured cells, using locked nucleic acid (LNA)-modified oligonucleotides.

69 L-6((-)/(-))) mice with locked nucleic acid (LNA)-modified phosphorothioate oligonucleotide complemen

70 delivery using a simple locked nucleic acid (LNA)-polymer conjugate that assembles into spherical mic

71 building blocks such as locked nucleic acid (LNA).

72 inds PS-ASOs containing locked-nucleic-acid (LNA) or constrained-ethyl-bicyclic-nucleic-acid ((S)-cEt

73 DNA/locked nucleic acids (LNA) AMOs synthesized with a phosphorothioate backbone a

74 tide probes containing locked nucleic acids (LNA) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide

75 rected mutagenesis and locked nucleic acids (LNA) complementary to defined domains of SL9266 and its

76 with modification with locked nucleic acids (LNA) increased conformational stability and nuclease res

77 Locked nucleic acids (LNA) show remarkable affinity and specificity against na

78 We have used locked nucleic acids (LNA) to engineer novel molecular beacons (MBs) for long-

79 dies, using anti-sense locked-nucleic acids (LNAs) and synthetic RNA mimetics, respectively, revealed

80 el antigens containing locked nucleic acids (LNAs) as targets for antibodies.

81 Me ribonucleotides and locked nucleic acids (LNAs) at selected sites.

82 scribe a unique use of locked nucleic acids (LNAs) for studying nuclear long noncoding RNA, an RNA su

83 ucleotides (ASOs) with locked nucleic acids (LNAs) improve target affinity, RNase H activation and st

84 The presence of locked nucleic acids (LNAs) in the ssODNs at mismatching bases, or also at dir

85 herapeutic delivery of locked nucleic acids (LNAs) targeting BCAR4 attenuated YAP-dependent glycolysi

86 herapeutic delivery of locked nucleic acids (LNAs) targeting BCAR4 strongly suppresses breast cancer

87 reatment of cells with locked nucleic acids (LNAs) targeting these miRNAs inhibits metastasis to mult

88 we introduced Invader locked nucleic acids (LNAs), i.e., double-stranded probes that are activated f

89 ence signal intensity between the beta-actin LNA probe and a biotinylated, nonspecific control LNA we

90 se-tolerability studies in mice administered LNA-modified ASOs.

91 uences with DNA/LNA alternating bases or all LNA bases were able to resist nonspecific protein bindin

92 Some, but not all, LNA containing oligonucleotides increased potency for re

93 sed exclusively of locked nucleic acids (all-LNAs) complementary to CUG repeats, as potential therape

94 Our data strongly indicate that short all-LNAs complementary to CUG repeats are a promising therap

95 The all-LNAs had low impact on the cellular level of CUG(exp)-co

96 skeletal muscles of DM1 mouse model the all-LNAs induced the reduction of the number and size of CUG

97 onstrated that very short, 8- or 10-unit all-LNAs effectively bound the CUG repeat RNA and prevented

98 othesized that enhanced affinity might allow LNAs to recognize chromosomal DNA inside human cells and

99 general, and the N2'-functionalized 2'-amino-LNA derivatives in particular, showed improved duplex th

100 hydrocarbon (PAH) dyes attached to 2'-amino-LNA monomers are incorporated at four stations of the sy

101 In addition, the 2'-amino-LNA-T derivatives induced remarkable 3'-exonuclease resi

102 tose-modified thymidine, LNA-T, and 2'-amino-LNA-T nucleosides were synthesized, converted into the c

103 ionalized thymine and 5-methylcytosine amino-LNA phosphoramidites from these key intermediates, respe

104 In contrast, LNA-3' and LNA-Even did not improve read lengths or C(T).

105 These DNA and LNA/DNA sequences showed improved binding in enzyme-link

106 The structures of the DNA and LNA:DNA hybrids are tentatively assigned to B- or A-type

107 range of backbone structures (RNA, DNA, and LNA templates and RNA and DNA primers) and two types of

108 y to completion on short homopolymer RNA and LNA templates, which favor an A-type duplex geometry.

109 est this hypothesis, we synthesized antigene LNAs (agLNAs) complementary to sequences within the prom

110 locked nucleic acid (LNA)-modified antimiR (LNA-antimiR-34) can provide therapeutic benefit in mice

111 of the standard Linear Noise Approximation (LNA) to remain uniformly accurate for long times, still

112 roperties for complementary nucleic acids as LNA.

113 ntaining high affinity modifications such as LNA, hepatotoxicity can occur as a result of unintended

114 otides containing locked nucleic acid bases (LNAs) have increased affinity for complementary DNA sequ

115 s new design adds locked nucleic acid bases (LNAs) to the beacon structure, resulting in a LNA molecu

116 nucleic acid (LNA) based molecular beacons (LNA-MBs) for a variety of applications including analysi

117 a DNA stretch less than three bases between LNA bases was able to block RNase H function.

118 alized to high affinity modifications beyond LNA.

119 cells by hybridization with a biotinylated, LNA-modified probe.

120 AE residues, the conformationally locked BNA/LNA ribose with a 2'-O,4'-C-methylene bridge, or the 2'-

121 hen loaded in a shear orientation, the bound LNA oligomers were measured to be two orders of magnitud

122 Inhibition of miR-34 by LNA-34a significantly reduced miR-34a expression and blo

123 perature; binding of SSB was not affected by LNA bases.

124 Decreasing uc.173 levels by LNA-anti-uc.173 in mice reduced renewal of the intestina

125 e purine or pyrimidine bases are replaced by LNA bases.

126 This combined method, called LNA flow-FISH, can be used for detection and quantificat

127 ity and biological activity with carbocyclic LNA (cLNA) analogs by replacing the 2'-oxygen atom in LN

128 So, which one to choose, LNA or GNA?

129 ionally, in MMR-proficient Escherichia coli, LNA modification of the ssODNs enabled effective single-

130 scrimination ability of the surface-confined LNA probes could be controlled by ionic modulations.

131 ce thermal stability of the surface-confined LNA-DNA duplexes, the nature of concentration dependence

132 tion of melting behavior of surface-confined LNA-DNA duplexes.

133 r non-clamp, or nucleotide-linker containing LNA-constructs.

134 Further, when we contrast LNA and GNA in the application of learning novel protein

135 In contrast, LNA-3' and LNA-Even did not improve read lengths or C(T)

136 robe and a biotinylated, nonspecific control LNA were used to determine optimal conditions for this t

137 detected and differentiated from the control LNA with high confidence (< 14% overlap between curves).

138 duced the infarct size compared with control LNA-treated pigs, which correlated with an improved ejec

139 Our method, called phase-corrected LNA (pcLNA) overcomes the main limitations of the standa

140 of microRNA-155 by a systemically delivered LNA-anti-miR attenuated cardiac infiltration by monocyte

141 A)-modified inhibitor of miR-192, designated LNA-anti-miR-192, in mouse models of diabetic nephropath

142 t allow for fair comparison of the different LNA and GNA outputs, as such measures do not exist.

143 with a 4 base-pair stem and alternating DNA/LNA bases is desirable for intracellular applications as

144 A highly specific biotinylated DNA/LNA molecular beacon (MB) probe was conjugated with gold

145 e systematically synthesized a series of DNA/LNA chimeric MBs and studied the effect of DNA/LNA ratio

146 A chimeric MBs and studied the effect of DNA/LNA ratio in MBs on their thermodynamics, hybridization

147 e significantly improved by lowering the DNA/LNA ratio in the probe, and most significantly, by havin

148 It was found that only MB sequences with DNA/LNA alternating bases or all LNA bases were able to resi

149 aperitoneal injections of a plasmid encoding LNA-anti-uc.173, to knock down endogenous uc.173.

150 : near the 5' end (LNA-5'), near the 3' end (LNA-3') and distributed throughout (LNA-Even).

151 three positional patterns: near the 5' end (LNA-5'), near the 3' end (LNA-3') and distributed throug

152 Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves a

153 to inhibit miR-34a alone as a comparison for LNA-antimiR-34.

154 further suggest a therapeutic potential for LNA-CTG in polyglutamine disorders.

155 Histopathological evaluation of tissues from LNA treated animals confirmed the hepatocellular involve

156 . conventional and C5-alkynyl-functionalized LNA and alpha-L-LNA pyrimidine monomers.

157 t, these properties render C5-functionalized LNA as a promising class of building blocks for RNA-targ

158 dified with four different C5-functionalized LNA cytidine and C8-functionalized LNA adenosine monomer

159 welve structurally diverse C5-functionalized LNA uridine (U) phosphoramidites were synthesized and in

160 ionalized LNA cytidine and C8-functionalized LNA adenosine monomers.

161 modified with C5-fluorophore-functionalized LNA-U monomers enable fluorescent discrimination of targ

162 Furthermore, LNA-anti-miR-192 attenuated proteinuria in these diabeti

163 nal cohort of mice subjected to MI was given LNA-antimiR-34a (15-mer) to inhibit miR-34a alone as a c

164 tilabeled quencher-free MBs based on Glowing LNA monomers is demonstrated (a) using in vitro transcri

165 These features render Glowing LNA as promising diagnostic probes for biomedical applic

166 and fluorescence properties of these Glowing LNA probes are efficiently modulated and optimized by ch

167 ined by substituting LNA with 2'-glycylamino-LNA, contributing a positive charge.

168 ockdown in mice treated with non-hepatotoxic LNA ASOs, while the levels of many unintended transcript

169 y efficacious hepatotoxic or non-hepatotoxic LNA ASOs.

170 ere reduced in mice treated with hepatotoxic LNA ASOs.

171 acids) 0.07% of LNA; "Low LNA" (0.4%); "High LNA" (4.8%); and a "DHA + EPA" diet, containing 0.4% of

172 results differentiate the effects of a High LNA diet from one with added EPA and DHA even though the

173 ared to the DHA + EPA diet, whereas the High LNA diet induced a less pronounced, but significant redu

174 r between 10 and 36 h when combined with IF; LNA-CISH requires 2 d.

175 ) analogs by replacing the 2'-oxygen atom in LNA with an exocyclic methylene group.

176 Improved outcome in LNA-antimiR-34-treated MI and TAC mice was accompanied b

177 Disease prevention in LNA-antimiR-34a treated DCM female mice was characterize

178 with similar efficiency as original Invader LNAs.

179 RNA, but less stabilizing than both alpha-L-LNA (2) and alpha-L-TriNA 1 (4).

180 A constrained tricyclic analogue of alpha-L-LNA (2), which contains dual modes of conformational res

181 ubstitution converts 8 into 2'-amino-alpha-L-LNA adenine intermediate 9, which after a series of nont

182 eotides (ONs) modified with 2'-amino-alpha-L-LNA adenine monomers W-Z.

183 with pyrene-functionalized 2'-amino-alpha-L-LNA adenine monomers X-Z display greatly increased affin

184 sequences containing this tricyclic alpha-L-LNA analogue (alpha-L-TriNA 2, 5) indicate that this mod

185 leic acid (LNA) and its diastereomer alpha-L-LNA are two interesting examples thereof.

186 her target specificity than 2'-amino-alpha-L-LNA benchmark probes.

187 of 2'-N-(pyren-1-yl)methyl-2'-amino-alpha-l-LNA monomers.

188 nd C5-alkynyl-functionalized LNA and alpha-L-LNA pyrimidine monomers.

189 alpha-L-LNA uridine monomers that are conjugated to small C5-alk

190 ionalized monomers based on 2'-amino-alpha-l-LNA, 2'-N-methyl-2'-amino-DNA, and RNA scaffolds.

191 TFOs form more stable triplexes than alpha-L-LNA-modified counterparts owing to slower triplex dissoc

192 c stability relative to conventional alpha-L-LNA.

193 article, we extend this strategy to alpha-L-LNA: i.e., one of the most interesting diastereomers of

194 N2'-Pyrene-functionalized 2'-amino-alpha-L-LNAs (locked nucleic acids) display extraordinary affini

195 r N2'-pyrene-functionalized 2'-amino-alpha-L-LNAs of considerable interest for DNA-targeting applicat

196 f N2'-pyrene-functionalized 2'-amino-alpha-L-LNAs.

197 There exist two NA categories: local (LNA) and global (GNA).

198 The n-3 Deficient and Low LNA diets caused a substantial deficit in PPI compared t

199 (as % total fatty acids) 0.07% of LNA; "Low LNA" (0.4%); "High LNA" (4.8%); and a "DHA + EPA" diet,

200 bases, was readily accessible to tiled 8-mer LNA and 15-mer DNA probes, whereas an unmodified version

201 dulloblastoma cells) passively took up 8-mer LNA-anti-miRs and specifically inhibited targeted microR

202 ion of the miR-17~92 cluster family by 8-mer LNA-anti-miRs might be considered for the treatment of S

203 derscore the utility of seed-targeting 8-mer LNA-antimiRs in the development of new therapeutic appro

204 Furthermore, in R6/2 mice, LNA-CTG blocked several pathogenic mechanisms caused by

205 , and 9 healthy controls (HCs) using miRCURY LNA Universal RT microRNA polymerase chain reaction pane

206 ssion of miR-183-96-182 cluster with miRCURY LNA miR inhibitors decreases the proliferation and migra

207 The LNA-MBs were made of a mixed LNA and DNA bases to have extremely high biostability.

208 delivery of a locked nucleic acid-modified (LNA-modified) antimiR oligonucleotide also failed to blo

209 ng 2'-methoxyethyl bases or noncomplementary LNAs.

210 onvergent synthesis of bicyclic nucleosides (LNA monomers) T, U, A, and C.

211 the first time that it is possible to obtain LNA-like (Locked Nucleic Acid 1) binding affinity and bi

212 containing (as % total fatty acids) 0.07% of LNA; "Low LNA" (0.4%); "High LNA" (4.8%); and a "DHA + E

213 ; and a "DHA + EPA" diet, containing 0.4% of LNA, 2% DHA, and 2% EPA.

214 that was paralleled by persistent binding of LNA-CTG to the expanded HTT exon 1 transgene.

215 r efforts have been devoted to the design of LNA derivatives that induce even higher binding affinity

216 findings have implications on the design of LNA molecular probes for intracellular monitoring applic

217 New guidelines are suggested for design of LNA probes, which significantly improve mismatch discrim

218 one of the most interesting diastereomers of LNA.

219 is effort we have investigated the effect of LNA on the performance of sequencing and PCR primers in

220 Effects of LNA modifications on mismatch discrimination were studie

221 vivo, as we observed complete eradication of LNA-antimiR-21-treated gliomas subjected to the presence

222 odified ONs, whereas C8-functionalization of LNA adenosines is detrimental to binding affinity and sp

223 s of LNA: i.e., through functionalization of LNA nucleobases.

224 trongly suggest that C5-functionalization of LNA pyrimidines is indeed a viable approach for improvin

225 recently introduced C5-functionalization of LNA uridines as an alternative and synthetically more st

226 ward this end, i.e., C5-functionalization of LNA uridines.

227 d delivery, but not intravenous infusion, of LNA-92a significantly (P<0.05) reduced the infarct size

228 on the length of the agLNA, the location of LNA bases, the number of LNA substitutions, and the loca

229 of this work has focused on modifications of LNA's oxymethylene bridge.

230 NA, the location of LNA bases, the number of LNA substitutions, and the location of the target sequen

231 Given the different outputs of LNA and GNA, when a new NA method is proposed, it is com

232 rs to optimize the biophysical properties of LNA through additional modification of the sugar skeleto

233 ach to improve the biophysical properties of LNA.

234 approach for modulation of the properties of LNA: i.e., through functionalization of LNA nucleobases.

235 The hybridization rates of LNA-MBs were significantly improved by lowering the DNA/

236 stablish structure-toxicity relationships of LNA-modified ASOs and decrease the likelihood of hepatot

237 t specificity, and/or enzymatic stability of LNA-modified ONs, whereas C8-functionalization of LNA ad

238 proved therapeutic index relative to that of LNA antisense oligonucleotides.

239 iscrimination attributes similar to those of LNA but greatly improved resistance to exonuclease diges

240 A triplet of LNA residues centered on the mismatch was generally foun

241 These data suggest that the mechanism of LNAs involves recognition of chromosomal DNA and that LN

242 ocked nucleic acid-modified oligonucleotide (LNA-antimiR-34a) at 6-7 weeks of age when the models dis

243 acids (also known as locked nucleic acid or LNA, and 2',4'-constrained ethyl [cET]).

244 ydrophobic 2' modifications, e.g. (S)-cEt or LNA, in the 5'-wing of the ASO.

245 In mice subjected to pressure overload, LNA-antimiR-34 improved systolic function and attenuated

246 tive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene

247 We evaluate prominent LNA and GNA methods on synthetic and real-world biologic

248 ng screening of fully phosphorothioated (PS)-LNA gapmer ASOs designed against the BACH1 transcript.

249 te Hsp90 protein enhances the activity of PS/LNA or PS/(S)-cEt ASOs, and imply that altering protein

250 Regional LNA-92a delivery reduces miR-92a levels and infarct size

251 against 3'-exonucleases relative to regular LNA.

252 onucleotide complementary to the CAG repeat (LNA-CTG) preferentially binds to mutant HTT without affe

253 Cellular uptake of soft LNA nanoparticles occurs rapidly within minutes as evide

254 m behind the hepatoxicity observed with some LNA-modified gapmers.

255 r improvements were obtained by substituting LNA with 2'-glycylamino-LNA, contributing a positive cha

256 We target LNAs at Xist RNA and show displacement from the X chromo

257 ly improved by applying the surface-tethered LNA probes, in comparison to the corresponding DNA probe

258 n start sites yielded better inhibition than LNAs targeting transcription factor binding sites or an

259 In vivo studies revealed that LNA(TM) anti-miR-130a could up-regulate the AQP4 M1 tran

260 Histochemistry revealed that LNA-92a increased capillary density but decreased leukoc

261 Our results show that LNA provides a simple, stable means to functionalize dsD

262 experiments using 2-aminopurine suggest that LNA modifications enhance base stacking of perfectly mat

263 These results suggest that LNA-CTGs promote neuroprotection by blocking the detrime

264 lves recognition of chromosomal DNA and that LNAs are bona fide antigene molecules.

265 We demonstrate that LNAs which block formation of the closed conformation in

266 The LNA bound to beta-actin was then stained using phycoeryt

267 The LNA-let-7 inhibitor decreased brain let-7 levels and par

268 The LNA-MBs were made of a mixed LNA and DNA bases to have e

269 The LNA-modified cap analogue was an efficient substrate for

270 After 24 h inside living cells, the LNA-MBs were still functional, demonstrating a greatly e

271 ing 50% formamide allow cells containing the LNA-bound mRNA to be detected and differentiated from th

272 ntly, by having a shared-stem design for the LNA-MB to prevent sticky-end pairing.

273 e speed and analytically tractability of the LNA.

274 nce to the increased A-form character of the LNA:RNA hybrid.

275 In the second step, the LNA-blocking approach is combined with a mutant-specific

276 It was found that the LNA bases in a MB stem sequence had a significant effect

277 analytical HPLC assay to determine that the LNA-modified 3 cap analogue was incorporated solely into

278 ing protein elF4E complex indicates that the LNA-modified cap-protein complex is more stable by 47.28

279 Importantly, these LNA nanoparticles knockdown survivin mRNA, an establishe

280 le organs, and therapeutic delivery of these LNAs strongly suppresses melanoma metastasis.

281 Though LNA has been used in many applications, formal design ru

282 3' end (LNA-3') and distributed throughout (LNA-Even).

283 Thus, LNAs provide a tool for studying an emerging class of re

284 Galactose-modified thymidine, LNA-T, and 2'-amino-LNA-T nucleosides were synthesized,

285 ified anti-miR oligonucleotides, termed tiny LNAs, that inhibit microRNA seed families expressed by m

286 activity but reduced toxicity as compared to LNA ASOs.

287 sts of magnetic nanoparticles, conjugated to LNA-based miR-10b antagomirs.

288 e mRNA transcripts commonly reduced by toxic LNA ASOs were generally not strongly associated with any

289 tion temperature is required for each unique LNA probe.

290 Oligonucleotide-based therapies using LNA-modified chemistries for modulating cardiac miRNAs i

291 In vitro, LNA modification of mismatches precluded binding of puri

292 In vitro, LNA-92a protected against hypoxia/reoxygenation-induced

293 cations were designed to investigate whether LNA antisense oligonucleotides (ASOs) have the potential

294 n contrast, inhibition of miR-34a alone with LNA-antimiR-34a (15-mer) provided no benefit in the MI m

295 Pre-treatment of BMCs with LNA-34a ex vivo significantly increased the therapeutic

296 the G-block of the diblock DNA hairpin with LNA bases results in a modest decrease in the base-to-ba

297 properties of oligonucleotides modified with LNA or other conformationally restricted monomers.

298 amer 2'-O-methyl oligonucleotide probes with LNA (locked nucleic acid) and 2,6-diaminopurine substitu

299 aco-2 and IEC-6 lines) were transfected with LNA-anti-uc.173 or uc.173 transgene.

300 plexes modified with C5-(3-aminopropyn-1-yl)-LNA-U monomer Z are particularly stable.