ライフサイエンスコーパス: LRP-1

1 LRP-1 also functions as a catabolic receptor for fibrone

2 LRP-1 also is implicated in integrin maturation.

3 LRP-1 expression on peripheral blood DCs was quantified

4 LRP-1 is not known to modulate the pathogenesis of aller

5 LRP-1, although abundant in brain microvessels in young

6 LRP-1-deficient macrophages, isolated from mice, demonst

7 LRP-1-deficient MEFs demonstrated increased Rac1 activat

8 LRP-1-deficient mouse embryonic fibroblasts lack a growt

9 sity lipoprotein receptor-related protein 1 (LRP-1) is a scavenger receptor that regulates adaptive i

10 ved that the LDL receptor-related protein 1 (LRP-1) is tyrosine phosphorylated in v-Src-transformed c

11 sity lipoprotein receptor-related protein 1 (LRP-1) receptor with receptor-associated protein (RAP) o

12 sity Lipoprotein Receptor-Related Protein 1 (LRP-1) receptor.

13 eceptor, the LDL receptor-related protein 1 (LRP-1).

14 eceptor, the LDL receptor-related protein 1 (LRP-1).

15 dies against LDL receptor-related protein-1 (LRP-1) and alpha(2)-macroglobulin (alpha(2)M).

16 r low-density lipoprotein-related protein-1 (LRP-1) from two smaller overlapping BACs ("BAC marriage"

17 sity lipoprotein receptor-related protein-1 (LRP-1) in NRK-49F cells, and this binding was competitiv

18 sity lipoprotein receptor-related protein-1 (LRP-1) is a catabolic receptor for extracellular matrix

19 sity lipoprotein receptor-related protein-1 (LRP-1) is a multifunctional receptor involved in recepto

20 sity lipoprotein receptor-related protein-1 (LRP-1) is expressed in adult sciatic nerve.

21 sity lipoprotein receptor-related protein-1 (LRP-1) mediates the endocytosis of multiple plasma membr

22 sity lipoprotein receptor-related protein-1 (LRP-1) plays a major role in TIMP-3 internalization.

23 ipoprotein (LDL) receptor-related protein-1 (LRP-1) to become enzymatically active.

24 kinase AXL, LDL receptor-related protein-1 (LRP-1), and RAN-binding protein 9 (RANBP9)--that mediate

25 ts receptor, LDL receptor-related protein-1 (LRP-1).

26 sity lipoprotein receptor-related protein-1 (LRP-1).

27 Although LRP-1 functions as an endocytic receptor for C1r and C1s

28 Although LRP-1 gene silencing did not inhibit CL16 cell dissemina

29 g Schwann cell signaling and migration by an LRP-1-dependent pathway.

30 1 phosphorylation in vivo, we constructed an LRP-1 minireceptor composed of the beta chain linked at

31 cell survival, whereas re-introduction of an LRP-1 minigene in a mouse LRP-1-deficient fibroblast cel

32 RAP, an LRP-1 antagonist, inhibits binding of 125I-TGF-beta1 and

33 al fibroblasts and myofibroblasts through an LRP-1-, Erk1/2-, p90RSK-, and Bad-dependent mechanism.

34 AXL and LRP-1 did not interact directly, but relied on RANBP9, w

35 t relied on RANBP9, which bound both AXL and LRP-1, to form the complex.

36 P-1-negative mouse embryonic fibroblasts and LRP-1-deficient smooth muscle cells.

37 cs indicate that Glut4, the Tf receptor, and LRP-1 are differentially processed both within the cell

38 eceptor, a member of the same gene family as LRP-1, with overlapping ligand-binding specificity.

39 nce antagonists of receptor function such as LRP-1 and LRP-2 antibodies and the 39-kDa receptor-assoc

40 lture model of antigen presentation, the AXL/LRP-1/RANBP9 complex was used by DCs to cross-present AC

41 nt of beta(1) integrin was unchanged because LRP-1-deficient cells retained increased amounts of beta

42 peptides shows that the interaction between LRP-1 and Shc is direct.

43 production of active heparanase by blocking LRP-1-mediated uptake of pro-heparanase and thereby decr

44 This response was blocked by LRP-1 gene silencing or by co-incubation with the LRP-1

45 the subsequent endocytosis of the complex by LRP-1 or LRP-2.

46 with receptor-associated protein (RAP) or by LRP-1 gene silencing.

47 volved in ECM modeling that are regulated by LRP-1.

48 olved in inflammation, that are regulated by LRP-1.

49 The cell surface receptor CD91/LRP-1 binds to immunogenic heat shock proteins (HSP) and

50 Ce-LRP-1 and Ce-LRP-2 possess a conserved intraluminal doma

51 the lipoprotein receptor-related proteins Ce-LRP-1 and Ce-LRP-2 and a cytoplasmic adaptor protein, Ce

52 Silencing of Schwann cell LRP-1 with siRNA decreased phosphorylated Akt and increa

53 embryonic fibroblasts (MEFs) and L929 cells, LRP-1 functions as a major regulator of Rac1 activation,

54 In uPAR-negative cells, LRP-1 neutralization does not affect Rac1 activation, an

55 ss from the brain largely via age-dependent, LRP-1-mediated transport that is influenced by alpha(2)M

56 rference screen using Caenorhabditis elegans LRP-1/megalin as a model for LDLR transport.

57 AP-D3 interfere with transport of endogenous LRP-1 to the cell surface in a dominant negative fashion

58 These results establish LRP-1 as a cell-signaling receptor for MMP-9, which may

59 h the observation that Schwann cells express LRP-1 at significant levels only after nerve injury.

60 In contrast, the k(ex) for LRP-1 was five times faster than Glut4 in basal cells, a

61 This identifies a novel role for LRP-1 as a negative regulator of DC-mediated adaptive im

62 factors identified here suggests a role for LRP-1 in determining blood vessel structure and in angio

63 nophilic asthma suggests a possible role for LRP-1 in modulating type 2-high asthma.

64 Furthermore, LRP-1-silenced cells expressed decreased levels of vascu

65 The megalin homologue LRP-1 is essential for growth and development in Caenorh

66 Human LRP-1 reversed the changes in protein expression associa

67 Expression of human LRP-1 in LRP-1-deficient MEFs reversed the shift in subc

68 Expression of human LRP-1 in LRP-1-deficient MEFs reversed the shift in subcellular b

69 JNK) blocked type III collagen expression in LRP-1-deficient MEFs, suggesting regulation of JNK activ

70 A substantial increase in LRP-1 expression was observed.

71 ) was mostly responsible for the increase in LRP-1 expression; this activity was reproduced by direct

72 nt protease mRNA expression was increased in LRP-1-deficient cells, as was expression of inducible ni

73 ptor, uPAR-AP/Endo-180, is also increased in LRP-1-deficient MEFs.

74 levels of cell surface beta(1) integrin, in LRP-1-deficient MEFs, were associated with decreased adh

75 t changes in cell signaling were observed in LRP-1-deficient murine embryonic fibroblasts.

76 inhibited NF-kappaB activity selectively in LRP-1-deficient cells.

77 ed into mature beta(1) (p125) more slowly in LRP-1-deficient cells.

78 Increased LRP-1 mRNA expression was observed locally at the injury

79 TNF-alpha also increased LRP-1 mRNA in Schwann cells in primary culture.

80 These findings show that tPA induces LRP-1 tyrosine phosphorylation, which in turn facilitate

81 After crush injury, LRP-1 is lost from the axoplasm and substantially upregu

82 ceptor responsible for ADAMTS-5 clearance is LRP-1.

83 mouse embryonic fibroblasts (PEA-13) lacking LRP-1, tPA failed to induce MMP-9 expression.

84 nervous system, did not bind to full-length LRP-1.

85 patients with eosinophilic asthma have lower LRP-1 expression than cells from healthy nonasthmatic su

86 Insulin increased k(en) for alpha-2-M/LRP-1 by 30%.

87 In basal cells, the k(en) for alpha-2-M/LRP-1 was similar to Glut4 but 5-fold slower than Tf.

88 (Tf) and alpha(2)-macroglobulin (alpha-2-M; LRP-1) were compared using quantitative flow cytometric

89 introduction of an LRP-1 minigene in a mouse LRP-1-deficient fibroblast cell line (PEA-13) restored t

90 Notably, LRP-1-mediated endocytosis of ADAMTS-5 is impaired in ch

91 The ability of LRP-1 to regulate expression of the factors identified h

92 ry mediators was explained by the ability of LRP-1 to suppress basal cell signaling through the I kap

93 To test the activity of LRP-1 in cancer progression in vivo, LRP-1 expression wa

94 ficiency; however, the endocytic activity of LRP-1 was not involved.

95 Neutralizing the activity of LRP-1 with receptor-associated protein (RAP) increased R

96 isease was assessed in mice with deletion of LRP-1 in CD11c(+) cells (Lrp1(fl/fl); CD11c-Cre) and by

97 esidues present in the cytoplasmic domain of LRP-1, only Tyr 63 is phosphorylated by v-Src in vivo or

98 protein containing the cytoplasmic domain of LRP-1, we show that LRP-1 is a direct substrate for v-Sr

99 ERK) that was responsible for the effects of LRP-1 on MEF migration.

100 1Lu cells selected for reduced expression of LRP-1 have an attenuated growth inhibitory response to T

101 Deletion or knockdown of LRP-1 abolished tPA-mediated cell survival, whereas re-i

102 t express very low or undetectable levels of LRP-1 when cultured in 21% O2 in vitro (standard cell cu

103 oximately 90% reduction in protein levels of LRP-1 without changes in its mRNA levels.

104 Absent or low levels of LRP-1, as with TbetaR-V, have been linked to the maligna

105 increase cAMP levels or inhibit migration of LRP-1-negative mouse embryonic fibroblasts and LRP-1-def

106 The role of LRP-1 in modulating HDM-induced airways disease was asse

107 ly slowed down after 10 h due to shedding of LRP-1 from the cell surface and formation of soluble LRP

108 osine phosphorylation on the beta subunit of LRP-1, which was followed by the activation of Mek1 and

109 1) integrin was most profoundly dependent on LRP-1 only after the cell cultures became confluent.

110 re expression of normal RAP has no effect on LRP-1 transport.

111 or with receptor-associated protein (RAP) or LRP-1 siRNA abolished the ApoE effects.

112 s present in the cell that can phosphorylate LRP-1.

113 Tyrosine-phosphorylated LRP-1 associates with Shc, a PTB and SH2 domain containi

114 In the peripheral nervous system (PNS), LRP-1 is robustly expressed by Schwann cells only after

115 ing Lys(1370) and Lys(1374), which precludes LRP-1 binding.

116 38B treatment, the receptor guidance protein LRP-1, which is required for endosomal recycling of the

117 aling pathway: LDL receptor-related protein (LRP-1) and decorin.

118 ensity lipoprotein receptor-related protein (LRP-1) and/or other cell surface receptors.

119 ensity lipoprotein receptor-related protein (LRP-1) binds and mediates the endocytosis of multiple li

120 ensity lipoprotein receptor-related protein (LRP-1) functions in endocytosis and in cell signaling di

121 ensity lipoprotein receptor-related protein (LRP-1) is an endocytic receptor for diverse proteins, in

122 ensity lipoprotein receptor-related protein (LRP-1), an endocytic receptor with cell signaling activi

123 analysis of a gp330/megalin-related protein, LRP-1, has been undertaken in Caenorhabditis elegans.

124 The adoptive transfer of HDM-pulsed LRP-1-deficient CD11b(+) DCs into WT mice generated a si

125 In addition to Rac1, LRP-1 suppressed activation of extracellular signal-regu

126 In rat kidney fibroblasts, tPA induced rapid LRP-1 tyrosine phosphorylation and enhanced beta1 integr

127 ion of the Abeta vascular clearance receptor LRP-1, and protection from the deleterious effects of Ab

128 M1 macrophage survival through its receptor LRP-1-mediated novel signaling cascade involving Erk1/2,

129 ine that binds to the cell membrane receptor LRP-1, induces its tyrosine phosphorylation, and trigger

130 cell migration through the surface receptor LRP-1 (LDL receptor-related protein 1)/CD91.

131 lized with LRP-1, and tPA deficiency reduced LRP-1/beta1 integrin interaction and myofibroblast activ

132 Furthermore, the reduced LRP-1 expression by circulating myeloid DCs in patients

133 These activities require LRP-1.

134 We found that AXL bound ACs, but required LRP-1 to trigger internalization, in murine CD8alpha+ DC

135 Similarly, LRP-1 expression by CD11b(+) lung DCs was significantly

136 om the cell surface and formation of soluble LRP-1 (sLRP-1)-TIMP-3 complexes.

137 virus-1 infection, mice lacking DC-specific LRP-1, AXL, or RANBP9 had increased AC accumulation, def

138 To study LRP-1 phosphorylation in vivo, we constructed an LRP-1 m

139 alpha action, or removal of the cell surface LRP-1 receptor for secreted Hsp90alpha reduces the tumor

140 We hypothesized that LRP-1 may down-regulate cell surface beta(1) by promotin

141 These observations indicate that LRP-1 is related to megalin not only structurally but al

142 We propose that LRP-1 suppresses expression of inflammatory mediators in

143 We show that LRP-1 deficiency in murine embryonic fibroblasts (MEFs)

144 he cytoplasmic domain of LRP-1, we show that LRP-1 is a direct substrate for v-Src in vitro.

145 In this study, we show that LRP-1 is abundantly expressed in severe combined immunod

146 We show that LRP-1 is associated with endothelial transcytosis that d

147 cause recent studies in rodents suggest that LRP-1 inhibits inflammation, we conducted activity-based

148 Our observations suggest that LRP-1 may be involved in normal and malignant signal tra

149 enocopy the lrp-1 mutations, suggesting that LRP-1 is a receptor for sterols that must be endocytosed

150 essed the growth factor carrier site and the LRP-1 recognition domain (RBD) as separate GST fusion pr

151 Both apoE and pro-heparanase bind the LRP-1.

152 s binding was competitively abrogated by the LRP-1 antagonist, the receptor-associated protein.

153 osphorylation, which in turn facilitates the LRP-1-mediated recruitment of beta1 integrin and downstr

154 ta1 integrin recruitment by facilitating the LRP-1/beta1 integrin complex formation.

155 l death was induced in vivo by injecting the LRP-1 antagonist, receptor-associated protein, into axot

156 These results suggest that the LRP-1/TbetaR-V/IGFBP-3 receptor is required for the grow

157 gene silencing or by co-incubation with the LRP-1 antagonist, receptor-associated protein.

158 Thus, LRP-1 dictates physiological and pathological catabolism

159 Thus, LRP-1 regulates two signaling proteins in the same cell

160 cells is immunoprecipitated by antibodies to LRP-1 and TbetaR-V.

161 acids, is responsible for decorin binding to LRP-1 and subsequent TGF-beta-dependent signaling.

162 tracellular pathway by which apoE binding to LRP-1 results in inhibition of smooth muscle cell migrat

163 were blocked by inhibiting MMP-9-binding to LRP-1 with receptor-associated protein (RAP) or by LRP-1

164 teases, the thiol ester bonds, or binding to LRP-1.

165 ctivation as a consequence of its binding to LRP-1.

166 etaR-V was recently found to be identical to LRP-1.

167 we demonstrate that TbetaR-V is identical to LRP-1/alpha2M receptor as shown by MALDI-TOF analysis of

168 In mouse obstructed kidney, tPA, LRP-1, and MMP-9 were concomitantly induced in the renal

169 The ability of FP6 to trigger LRP-1-dependent cell signaling in PC12 cells was reprodu

170 its internalization; however, unexpectedly, LRP-1 expression was associated with a substantial incre

171 ith insulin, (Q3A4Y15L16) IGF-I, or TbetaR-V/LRP-1 antagonists.

172 and IRS-2 are key molecules for the TbetaR-V/LRP-1-mediated growth inhibitory signaling cascade.

173 vity of LRP-1 in cancer progression in vivo, LRP-1 expression was silenced in CL16 cells with short h

174 rawal or TNF-alpha, to a greater extent when LRP-1 was silenced.

175 roteases accumulate at increased levels when LRP-1 is absent.

176 antly increased cell death was observed when LRP-1-silenced CL16 cells were exposed to CoCl2, which m

177 We sought to assess whether LRP-1 expression by dendritic cells (DCs) modulates adap

178 f the present study was to determine whether LRP-1 regulates cell surface levels of the beta(1) integ

179 To test whether LRP-1 expression in vivo may be explained by hypoxia in

180 tion of JNK activity as a mechanism by which LRP-1 controls mRNA expression.

181 The mechanism by which LRP-1 inhibits NF-kappaB activity involves down-regulati

182 These results suggest mechanisms by which LRP-1 may facilitate the development and growth of cance

183 c1 activation, and other mechanisms by which LRP-1 may regulate cell migration are not unmasked.

184 These studies support a model in which LRP-1 either directly or indirectly promotes maturation

185 e cells formed tumors in SCID mice, in which LRP-1 expression remained silenced.

186 These results support a model in which LRP-1 functions as a pro-survival receptor in Schwann ce

187 A mutagen-treated CHO cell line, in which LRP-1 is expressed but retained in the secretory pathway

188 hanges in protein expression associated with LRP-1 deficiency; however, the endocytic activity of LRP

189 ion of H1299 human lung carcinoma cells with LRP-1 cDNA restores the growth inhibitory response.

190 e injury, tPA and alpha-SMA colocalized with LRP-1, and tPA deficiency reduced LRP-1/beta1 integrin i

191 ated increased Rac1 activation compared with LRP-1-expressing MEFs, and this property was reversed by

192 in that are responsible for interaction with LRP-1 and are involved in TGF-beta-dependent binding and

193 and LRR5 participate in the interaction with LRP-1 and TGF-beta as well as in its dependent signaling

194 f MMP-9, which mediates the interaction with LRP-1, blocked MMP-9-induced cell signaling and migratio