ライフサイエンスコーパス: M. abscessus

1 acterial species Mycobacterium fortuitum and M. abscessus.

2 The isolates were later identified as M. abscessus.

3 PL, occur during chronic lung infection with M. abscessus.

4 the erm(41) genotype is a useful adjunct for M. abscessus.

5 suitable to test antibiotic activity against M. abscessus.

6 ne showed promising in vivo activity against M. abscessus.

7 cterium avium complex (MAC) and 69 (36%) for M. abscessus.

8 s observed in M. marinum and the smallest in M. abscessus.

9 ty of a series of indolecarboxamides against M. abscessus.

10 f mycobacteria including M. tuberculosis and M. abscessus.

11 isotopic shifts from incorporation of 15N in M. abscessus.

12 nogenum varied from those of M. chelonae and M. abscessus.

13 5 isolates of M. chelonae and 25 isolates of M. abscessus.

14 not distinguish Mycobacterium chelonae from M. abscessus.

15 gene sequencing to identify M. chelonae and M. abscessus.

16 rentiate M. immunogenum from M. chelonae and M. abscessus.

17 gesting that this clone is a subgroup within M. abscessus.

18 ical isolates identified 2 clonal strains of M. abscessus; 1 clone was isolated from water sources at

19 haracterized clinical isolates comprising 29 M. abscessus, 15 M. massiliense, and 2 M. bolletii isola

20 ) for rapidly growing mycobacteria (98% were M. abscessus), 78% (29 of 37) for M. kansasii, and 26% (

21 dividuals with cystic fibrosis (CF), in whom M. abscessus accelerates inflammatory lung damage, leadi

22 A total of 82 isolates (58 M. abscessus and 24 M. chelonae isolates) were tested bl

23 methods, 46 of the isolates were found to be M. abscessus and 29 were identified as M. chelonae.

24 erm(41) sequencing of 285 M. abscessus and 45 M. chelonae isolates was compared to

25 tial sequencing) is required for division of M. abscessus and closely related species.

26 s previously identified as being M. chelonae/M. abscessus and identified M. massiliense from isolates

27 ting of M. fortuitum against clarithromycin; M. abscessus and M. chelonae against the aminoglycosides

28 hould be able to easily identify isolates of M. abscessus and M. chelonae.

29 nt may be helpful for distinguishing between M. abscessus and M. chelonae.

30 ) genome, regions that discriminated between M. abscessus and M. massiliense were identified through

31 and three pairs of closely related strains: M. abscessus and M. massiliense, M. mucogenicum and M. p

32 nchoscopes and endoscopic cleaning machines (M. abscessus) and contaminated hospital water supplies (

33 submitted as M. chelonae were identified as M. abscessus, and one isolate submitted as M. abscessus

34 ithmetic mean = 1.5% of hsp65 sequences) and M. abscessus (arithmetic mean = 0.006% of hsp65 sequence

35 Based on the M. abscessus ATCC 19977(T) genome, regions that discrimi

36 ighly effective at preventing infection with M. abscessus because it is a ubiquitous environmental sa

37 ndistinguishable from Mycobacterium chelonae/M. abscessus by partial 16S rRNA gene sequencing.

38 This demonstrates that the inability to type M. abscessus by PFGE is associated with a single clone o

39 fit of using thiourea-containing buffer with M. abscessus by studying 69 isolates not previously type

40 M. abscessus can transition between a noninvasive, biofi

41 cases (57%; odds ratio = 0.7, P < 0.05) and M. abscessus cases (51%; odds ratio = 0.5, P < 0.01) tha

42 peptidolipid in the outermost portion of the M. abscessus cell wall masks underlying cell wall lipids

43 this provides an explanation whereby initial M. abscessus colonization of abnormal lung airways escap

44 e sequencing (26 isolates of the M. chelonae-M. abscessus complex and 64 remaining isolates, includin

45 methods differentiate between members of the M. abscessus complex.

46 drug susceptibility testing, all isolates of M. abscessus exhibited resistance to tobramycin (MIC of

47 M. abscessus exists as either a glycopeptidolipid (GPL)

48 Conversely, M. abscessus expressing GPL does not stimulate expressio

49 Blood cultures grew M. abscessus for all patients, and admission peripheral

50 The release of M. abscessus from apoptotic macrophages initiated the fo

51 ory-confirmed colonization or infection with M. abscessus from January 2013 through December 2015.

52 on is facilitated by biofilm formation, with M. abscessus glycopeptidolipids playing an important rol

53 the M. avium complex (MAC), the M. chelonae-M. abscessus group (MCAG), the M. fortuitum group (MFG),

54 m abscessus (M. abscessus sensu lato, or the M. abscessus group) comprises three closely related taxa

55 ification within 24 h after isolation of the M. abscessus group.

56 , including M. immunogenum, M. chelonae, and M. abscessus, have been associated with nosocomial infec

57 The incidence rate of M. abscessus increased from 0.7 cases per 10000 patient-

58 of cording in the in vivo physiopathology of M. abscessus infection and emphasizes cording as a mecha

59 es from nine non-CF patients with persistent M. abscessus infection were characterized by colony morp

60 he 3 drugs usually combined for treatment of M. abscessus infection, cefoxitin was the most active be

61 Alternative routes of acquisition of M. abscessus infection, in particular the environment, r

62 nical isolates, we show that the majority of M. abscessus infections are acquired through transmissio

63 ited chemical structure class active against M. abscessus infections with promising translational dev

64 The same has been observed for M. abscessus infections, which are very difficult to tre

65 he differentiation of these two species from M. abscessus is difficult and relies on the sequencing o

66 It appears that M. abscessus is transported to the CNS within macrophage

67 ng activity in vitro against a wide panel of M. abscessus isolates and in infected macrophages.

68 ormed whole-genome sequencing of 11 clinical M. abscessus isolates derived from eight U.S. patients w

69 ole-genome sequencing data demonstrated that M. abscessus isolates from 16 patients were unrelated, d

70 The M. abscessus isolates from case patients and the supplem

71 Whole-genome sequencing was applied to 27 M. abscessus isolates from the 20 patients in this cohor

72 Previous population studies of clinical M. abscessus isolates utilized multilocus sequence typin

73 led to the unambiguous identification of 26 M. abscessus isolates, 7 M. massiliense isolates, and 2

74 ity testing by Etest of four carbapenems for M. abscessus isolates.

75 gordonae, and 5 M. chelonae group (all were M. abscessus) isolates.

76 d and mitigated a 2-phase clonal outbreak of M. abscessus linked to hospital tap water.

77 a rough, wild-type human clinical isolate of M. abscessus (M. abscessus-R) and a smooth, attenuated m

78 1) PCR for straightforward identification of M. abscessus, M. massiliense, and M. bolletii and the as

79 cation and typing of 42 clinical isolates of M. abscessus, M. massiliense, and M. bolletii from patie

80 ve PCR-based method for distinguishing among M. abscessus, M. massiliense, and M. bolletii.

81 solates with ambiguous species identities as M. abscessus-M. massiliense by rpoB, hsp65, and secA seq

82 d overscores resistance and that isolates of M. abscessus/M. chelonae from CF patients are more likel

83 were used to identify 75 isolates as either M. abscessus or M. chelonae that were originally submitt

84 We studied 118 strains of M. abscessus previously studied by PFGE by randomly ampl

85 observed that the increased virulence of the M. abscessus R variant compared with the S variant corre

86 oblast-mycobacterium microcolony assay, with M. abscessus-R exhibiting growth characteristics similar

87 ear phagocyte aggregates develop at sites of M. abscessus-R infection, but are absent with M. abscess

88 We have found that M. abscessus-R is able to persist and multiply in a muri

89 M. abscessus-R is able to persist and multiply in human

90 M. abscessus-R resides in a phagosome typical for pathog

91 type human clinical isolate of M. abscessus (M. abscessus-R) and a smooth, attenuated mutant (M. absc

92 Recently, two new M. abscessus-related species, M. massiliense and M. boll

93 The M. abscessus rough (R) variant, devoid of cell-surface g

94 ly reported for virulent M. tuberculosis and M. abscessus-S exhibiting growth characteristics similar

95 . abscessus-R infection, but are absent with M. abscessus-S infection.

96 rsist and multiply in human monocytes, while M. abscessus-S is deficient in this ability.

97 In contrast, M. abscessus-S resides in a "loose" phagosome with the p

98 conclude that a mutation has occurred in the M. abscessus-S variant which has altered the ability of

99 bscessus-R) and a smooth, attenuated mutant (M. abscessus-S) which spontaneously dissociated from the

100 ine pulmonary infection model in contrast to M. abscessus-S, which is rapidly cleared.

101 Mycobacterium abscessus (M. abscessus sensu lato, or the M. abscessus group) comp

102 taxonomic statuses are under revision, i.e., M. abscessus sensu stricto, Mycobacterium bolletii, and

103 Mycobacterium immunogenum, M. chelonae, and M. abscessus, showed various susceptibilities to the glu

104 study we demonstrate that rough variants of M. abscessus stimulate the human macrophage innate immun

105 e report herein the draft genome sequence of M. abscessus strain 47J26.

106 y explores the genomic diversity of clinical M. abscessus strains from multiple continents and provid

107 PD-PCR can be used for genetic comparison of M. abscessus strains, including strains which cannot be

108 acterium abscessus subsp. bolletii (n = 24), M. abscessus subsp. abscessus (n = 6), Mycobacterium for

109 he tedizolid MIC90 values for 81 isolates of M. abscessus subsp. abscessus and 12 isolates of M. absc

110 icated the presence of two clonal groups for M. abscessus subsp. abscessus and five clonal groups for

111 larithromycin susceptibility breakpoints for M. abscessus subsp. abscessus be changed from </=2 to </

112 on the usefulness of macrolides for treating M. abscessus subsp. abscessus infections.

113 scessus subsp. massiliense and 15% to 20% of M. abscessus subsp. abscessus isolates renders these spe

114 of closely related U.S. and Western European M. abscessus subsp. abscessus isolates that are genetica

115 Sequencing of the erm gene of M. abscessus subsp. abscessus will predict inducible mac

116 eating approximately 20% of U.S. isolates of M. abscessus subsp. abscessus.

117 . peregrinum and a nonfunctional erm gene in M. abscessus subsp. massiliense and 15% to 20% of M. abs

118 obacterium abscessus subsp. abscessus and 13 M. abscessus subsp. massiliense isolates identified by w

119 bscessus subsp. abscessus and 12 isolates of M. abscessus subsp. massiliense were 8 mug/ml and 4 mug/

120 All 50 isolates were typed as M. abscessus subspecies abscessus and were clonally rela

121 patients with genetically related strains of M. abscessus that had been previously typed by variable-

122 The intrinsic resistance of M. abscessus to most commonly available antibiotics seri

123 th smear patterns were identical to those of M. abscessus type strain ATCC 19977, which had a nonsmea

124 Both M. abscessus variants also have distinctive growth patte

125 that respiratory epithelial cells respond to M. abscessus variants lacking GPL with expression of IL-

126 s M. abscessus, and one isolate submitted as M. abscessus was found to be M. chelonae.

127 Previously, M. abscessus was thought to be independently acquired by

128 only in M. chelonae), and cefoxitin (only in M. abscessus) was shown.

129 h incident NTM infections from either MAC or M. abscessus were less likely to have had chronic azithr

130 Several bacterial species, including M. abscessus, were cultured from an opened multidose sup

131 Five outbreaks due to M. abscessus which gave broken DNA by PFGE gave evaluabl

132 have not demonstrated cross-transmission of M. abscessus within our hospital, except between 1 sibli