ライフサイエンスコーパス: M. catarrhalis

1 M. catarrhalis and H. influenzae colonization of the air

2 M. catarrhalis DNA microarrays containing 70-mer oligonu

3 M. catarrhalis has a growth requirement for arginine; th

4 M. catarrhalis has a putative oligopeptide permease ABC

5 M. catarrhalis HumA expressed on the surface of an Esche

6 M. catarrhalis is a strict human respiratory pathogen, a

7 M. catarrhalis likely causes approximately 10% of exacer

8 M. catarrhalis serum resistance was dramatically decreas

9 M. catarrhalis strain O35E uspA1 genes that contained wi

10 M. catarrhalis strains are frequently resistant to the b

11 M. catarrhalis usually resists complement-mediated serum

12 More than 100 M. catarrhalis genes were upregulated in vivo, including

13 /Th17 response with high levels of IL-1beta (M. catarrhalis, P = 2.2 x 10(-12); H. influenzae, P = 7.

14 , and macrophage inflammatory protein-1beta (M. catarrhalis, P = 1.6 x 10(-11); H. influenzae, P = 2.

15 Additionally, 200 M. catarrhalis genes were found to be downregulated when

16 Streptococcus pneumoniae (range, 39%-57%), M. catarrhalis (range, 63%--69%), and S. aureus (range,

17 Although little is known about M. catarrhalis pathogenesis, our laboratory has previous

18 tations were constructed in three additional M. catarrhalis strains: 012E, TTA37, and 046E.

19 also showed reduced lung inflammation after M. catarrhalis infections.

20 atarrhalis, is a protective antibody against M. catarrhalis.

21 MP B1 is a potential vaccine antigen against M. catarrhalis infections.

22 ctive candidate as a vaccine antigen against M. catarrhalis.

23 l resistance and a vaccine candidate against M. catarrhalis.

24 may be a potential vaccine candidate against M. catarrhalis.

25 PRELP enhances host innate immunity against M. catarrhalis through increasing complement-mediated at

26 accines or therapeutic interventions against M. catarrhalis infections.

27 lones (MICs, </=0.03 to 2 microg/ml) against M. catarrhalis.

28 related with its bactericidal titers against M. catarrhalis and bacterial CFU in lungs.

29 ial candidate for a bivalent vaccine against M. catarrhalis and NTHi infections.

30 irst promising peptide-based vaccine against M. catarrhalis Immunoinformatics predicts that it should

31 rial surface structures are expressed by all M. catarrhalis clinical isolates evaluated.

32 . influenzae, P = 7.1 x 10(-10)), TNF-alpha (M. catarrhalis, P = 1.5 x 10(-9); H. influenzae, P = 5.9

33 e sequence of mclS is highly conserved among M. catarrhalis isolates and is predicted to encode a pro

34 s ATCC 43617 that was highly conserved among M. catarrhalis strains and which encoded a predicted pro

35 om COPD patients who had recently cleared an M. catarrhalis infection to serum samples collected prio

36 In this study, we constructed an M. catarrhalis norB mutant and showed that planktonic gr

37 refractory to transposon mutagenesis, so an M. catarrhalis strain was constructed that was both able

38 ity cutoffs were not found for S. aureus and M. catarrhalis, and a lack of confirmed case data limite

39 n and were positive for both Haemophilus and M. catarrhalis.

40 </= 0.5-1 microgram/mL and H. influenzae and M. catarrhalis MICs = 2-4 microgram/mL.

41 the gram-negative bacteria H. influenzae and M. catarrhalis.

42 solates), H. influenzae (1545 isolates), and M. catarrhalis (456 isolates).

43 s against H. influenzae = 4 microgram/mL and M. catarrhalis = 2 microgram/mL.

44 008 microg/mL; MIC(90), 0.015 microg/mL) and M. catarrhalis (MIC(50), 0.06 microg/mL; MIC(90), 0.12 m

45 uenzae, Actinobacillus pleuropneumoniae, and M. catarrhalis.

46 e was inhibited when MAb 8E7 was absorbed by M. catarrhalis serotype A LOS, indicating that the M. ca

47 cine antigen to prevent infections caused by M. catarrhalis.

48 The potential role of TFP in colonization by M. catarrhalis was further investigated using in vivo st

49 y of the complete LOS glycoform expressed by M. catarrhalis 7169.

50 rface-exposed epitope of OMP B1 expressed by M. catarrhalis 7169.

51 uation of global transcriptome expression by M. catarrhalis cells in vivo.

52 of the production of lysozyme inhibitors by M. catarrhalis.

53 epithelial cells relevant to pathogenesis by M. catarrhalis (Chang, HEp2, A549, and/or 16HBE14o(-)).

54 elial cell lines relevant to pathogenesis by M. catarrhalis, including NCIH292 lung cells, middle ear

55 studies suggest type IV pilus production by M. catarrhalis is constitutive and ubiquitous, although

56 the iron acquisition mechanisms utilized by M. catarrhalis.

57 g of iron acquisition mechanisms utilized by M. catarrhalis.

58 he expression of serum resistance by certain M. catarrhalis strains.

59 ded ORFs encoding several well-characterized M. catarrhalis surface proteins including Hag, McaP, and

60 nary disease (COPD) who acquired and cleared M. catarrhalis.

61 tained from adults with COPD who had cleared M. catarrhalis from the respiratory tract following infe

62 PD who had acquired and subsequently cleared M. catarrhalis from their respiratory tracts were studie

63 r activity in immunogenicity and in clearing M. catarrhalis from mouse lungs.

64 ctional expression of SOD activity by cloned M. catarrhalis sodA.

65 Sequence analysis of the cloned M. catarrhalis 7169 DNA fragment revealed an open readin

66 TFP-deficient M. catarrhalis bacteria exhibit diminished adherence to

67 This newly described M. catarrhalis protein, termed HumA, is capable of direc

68 hat were cross-reactive towards 24 different M. catarrhalis isolates.

69 UTR) of the uspA2 genes in several different M. catarrhalis strains were shown to contain various num

70 ra were cross-reactive towards six different M. catarrhalis isolates and promoted bacterial clearance

71 pression of aniA and norB in three different M. catarrhalis strains, as measured by both DNA microarr

72 , and C followed by challenge with different M. catarrhalis strains of three serotypes.

73 soluble cytoplasmic fraction from disrupted M. catarrhalis cells or in the spent culture supernatant

74 e evaluated further as a vaccine antigen for M. catarrhalis.

75 eptococcus, and 10 (28.6%) were positive for M. catarrhalis.

76 tilization system previously undescribed for M. catarrhalis, thus providing another mechanism of iron

77 ty of generating large quantities of CD from M. catarrhalis for vaccine use, the CD gene from O35E wa

78 Analysis of total RNA extracted from M. catarrhalis ATCC 43617 cells grown under iron-replete

79 in the spent culture supernatant fluid from M. catarrhalis.

80 ays were then used to analyze total RNA from M. catarrhalis cells grown in a continuous-flow biofilm

81 spA1 mRNA was readily detectable in RNA from M. catarrhalis isolates that had 10 G residues in their

82 icated that the molecular mass of UspA1 from M. catarrhalis O35E was 83,500 +/- 116 Da.

83 al clearance of both O35E and a heterologous M. catarrhalis isolate, TTA24.

84 cted with the CopB protein of the homologous M. catarrhalis strain in Western blot analysis and bound

85 anscription-PCR (RT-PCR) analyses identified M. catarrhalis genes whose expression was affected by ox

86 asured in whole-cell lysates, was ablated in M. catarrhalis 2951 galE.

87 , suggesting that nitric oxide catabolism in M. catarrhalis is accomplished primarily by the norB gen

88 , pilT, and pilQ mutants were constructed in M. catarrhalis strain 7169.

89 an important nutritional virulence factor in M. catarrhalis.

90 Inactivation of the mapA gene in M. catarrhalis strain O35E reduced the acid phosphatase

91 ogenic mclS mutant strains were generated in M. catarrhalis isolates O35E, O12E, and McGHS1 and conta

92 a demonstrate that the involvement of Hag in M. catarrhalis adherence to A549 and HMEE cells is conse

93 yltransferase genes (lgt) were identified in M. catarrhalis 7169, a strain that produces a serotype B

94 are components of a novel TPS identified in M. catarrhalis and suggest that these proteins may be in

95 regulator, was identified and inactivated in M. catarrhalis strain O35E, resulting in an increase in

96 was used to construct an isogenic mutant in M. catarrhalis 7169.

97 ctulosonic acid (KDO) biosynthetic operon in M. catarrhalis with the gene order pyrG-kdsA-eno.

98 hesis and function of these phospholipids in M. catarrhalis.

99 it termed pilin, which is encoded by pilA in M. catarrhalis.

100 ial clinical and geographic relationships in M. catarrhalis.

101 hat the MhaB proteins play distinct roles in M. catarrhalis adherence.

102 two-component signal transduction system in M. catarrhalis yielded a mutant unable to grow in liquid

103 e the human host and establish an infection, M. catarrhalis must be able to effectively attach to the

104 Isolation of a new strain of H. influenzae, M. catarrhalis, or S. pneumoniae was associated with a s

105 ein D of nontypeable Haemophilus influenzae, M. catarrhalis has become a high-priority pathogen in ot

106 lial cells and antibodies against it inhibit M. catarrhalis interactions with the receptor.

107 etion/insertion mutation was introduced into M. catarrhalis strain 2951.

108 The introduction of the same mutations into M. catarrhalis strain ETSU-4 showed that the growth of a

109 We report the construction of an isogenic M. catarrhalis kdsA mutant in strain 7169 by allelic exc

110 truction and characterization of an isogenic M. catarrhalis lpxA mutant in strain O35E.

111 An isogenic M. catarrhalis sodA mutant was constructed in strain 716

112 onstruction and characterization of isogenic M. catarrhalis O35E mutants demonstrated that the lack o

113 nt assay (ELISA), containing the three major M. catarrhalis serotypes together with a complete series

114 identified the potential adhesin gene mcaP (M. catarrhalis adherence protein).

115 conjugates provides protection against most M. catarrhalis strains by eliciting humoral and cellular

116 ace protein Hag in the adherence of multiple M. catarrhalis strains was examined.

117 analysis revealed that approximately 20% of M. catarrhalis strains apparently possess a uspA2H gene

118 ly with the ubiquitous surface protein A2 of M. catarrhalis.

119 lipA and lipB did not affect the ability of M. catarrhalis O35E to attach to a human bronchial epith

120 ously shown to be involved in the ability of M. catarrhalis to both attach to human cell lines in vit

121 of a lipoprotein essential to the ability of M. catarrhalis to persist in an animal model.

122 ously shown to be involved in the ability of M. catarrhalis to persist in the chinchilla nasopharynx

123 ritical component involved in the ability of M. catarrhalis to resist the bactericidal activity of hu

124 ivo resulted in a decrease in the ability of M. catarrhalis to survive in the chinchilla nasopharynx

125 tibodies were found to decrease adherence of M. catarrhalis to A549 human lung cells by up to 47% and

126 t another molecule mediates the adherence of M. catarrhalis to these two cell lines.

127 involved in the biosynthesis and assembly of M. catarrhalis LOS currently remain undefined.

128 was used to determine whether attachment of M. catarrhalis to human bronchial epithelial (HBE) cells

129 The general biology of M. catarrhalis, however, including the mechanisms utiliz

130 jor proteins involved in the biosynthesis of M. catarrhalis TFP and determined that the TFP expressed

131 The duration of carriage of M. catarrhalis was shorter with exacerbations compared w

132 ibodies made specifically during carriage of M. catarrhalis.

133 2 showed significantly enhanced clearance of M. catarrhalis from the lung compared to that in the con

134 train-specific protection after clearance of M. catarrhalis from the respiratory tract.

135 120 episodes of acquisition and clearance of M. catarrhalis in 50 patients; 57 (47.5%) of the acquisi

136 o OppA and resulted in enhanced clearance of M. catarrhalis in a mouse pulmonary clearance model.

137 or their antiserum on pulmonary clearance of M. catarrhalis in an aerosol challenge mouse model.

138 was found to enhance pulmonary clearance of M. catarrhalis in an animal model.

139 100 episodes of acquisition and clearance of M. catarrhalis.

140 Colonization densities of M. catarrhalis, S. aureus, and P. jirovecii are unlikely

141 may play a major role in the enhancement of M. catarrhalis clearance from lungs.

142 The uspA1 and uspA2 genes of M. catarrhalis O35E encode two different surface-exposed

143 These results indicate that growth of M. catarrhalis in a biofilm results in increased express

144 ether methylated arginine supports growth of M. catarrhalis is important in understanding fitness in

145 ke of basic amino acids to support growth of M. catarrhalis.

146 ding the 200-kDa protein (designated Hag) of M. catarrhalis strain O35E was subjected to nucleotide s

147 children, it is likely that the incidence of M. catarrhalis infections will continue to rise.

148 P binds the majority of clinical isolates of M. catarrhalis (n = 49) through interaction with the ubi

149 ow that the majority of clinical isolates of M. catarrhalis (n = 49), but not other tested bacterial

150 uencing of the genes in clinical isolates of M. catarrhalis.

151 rotypes A, B, and C) in clinical isolates of M. catarrhalis.

152 highly conserved among clinical isolates of M. catarrhalis.

153 oreover, COMP inhibits phagocytic killing of M. catarrhalis by human neutrophils.

154 adhesins, we generated a plasmid library of M. catarrhalis DNA fragments, which was introduced into

155 tion, serum IgG, IgM, and IgA against LOS of M. catarrhalis were detected.

156 8E7, which recognizes surface-exposed LOS of M. catarrhalis, is a protective antibody against M. cata

157 etermine if the lipooligosaccharide (LOS) of M. catarrhalis has a role in serum resistance, the UDP-g

158 own to be localized to the outer membrane of M. catarrhalis and was not detected either in the solubl

159 ses revealed that a HumA-deficient mutant of M. catarrhalis (7169::humA) is restricted for growth in

160 vious analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicated that UspA1 was invo

161 e distribution of modM alleles in a panel of M. catarrhalis strains, isolated from the nasopharynx of

162 or together, might exert on the phenotype of M. catarrhalis 035E.

163 The hag gene product of M. catarrhalis isolate O35E was expressed in the heterol

164 P) CD, a highly conserved surface protein of M. catarrhalis under consideration as a vaccine antigen,

165 This is the first report of M. catarrhalis ompCD mutants, and our findings demonstra

166 of arginine, a strict growth requirement of M. catarrhalis.

167 levant because an intracellular reservoir of M. catarrhalis is present in the human respiratory tract

168 be an important factor in the resistance of M. catarrhalis to the complement-mediated bactericidal e

169 ved in the expression of serum resistance of M. catarrhalis.

170 UspA2 is involved in the serum resistance of M. catarrhalis; this represents the first example of vit

171 Little is known about the role of M. catarrhalis in this common disease.

172 erized a substrate binding protein, SBP2, of M. catarrhalis.

173 formation were used to construct a series of M. catarrhalis O12E strains that differed only in the nu

174 acterial clearance of all three serotypes of M. catarrhalis strains in vaccinated mice, but also elev

175 oss-reactivity toward all three serotypes of M. catarrhalis under transmission electron microscopy.

176 e contribution of TFP to the early stages of M. catarrhalis colonization.

177 is available regarding the initial steps of M. catarrhalis pathogenesis, this organism must be able

178 nimal system for studying the early steps of M. catarrhalis pathogenesis.

179 An unfinished genome sequence of a strain of M. catarrhalis available in the GenBank database was ana

180 s study indicated that a wild-type strain of M. catarrhalis was very resistant to killing by exogenou

181 confirm that each of these three strains of M. catarrhalis expressed both UspA1 and UspA2 proteins.

182 onary clearance of different test strains of M. catarrhalis in mice.

183 ransporter that is present in all strains of M. catarrhalis tested.

184 The sequences of ompE from 16 strains of M. catarrhalis were determined, including the 4 strains

185 s the conservation of OMP E among strains of M. catarrhalis, 22 isolates were studied with eight mono

186 t OMP E is highly conserved among strains of M. catarrhalis, and preliminary studies indicate that th

187 omologous strain and heterologous strains of M. catarrhalis.

188 both homologous and heterologous strains of M. catarrhalis.

189 nd antigenic variation, the pilin subunit of M. catarrhalis appears to be more highly conserved as th

190 andidate antigen on the bacterial surface of M. catarrhalis.

191 se proteins were expressed on the surface of M. catarrhalis.

192 Binding of COMP correlates with survival of M. catarrhalis in human serum by inhibiting bactericidal

193 Testing of M. catarrhalis O35E katA and ahpC mutants for their abil

194 emonstrated that the zinc ABC transporter of M. catarrhalis is critical for invasion of respiratory e

195 e for the previously unidentified tropism of M. catarrhalis for ciliated NHBE cells.

196 A spontaneous UspA2H-negative variant of M. catarrhalis strain O46E, designated O46E.U2V, was fou

197 ations and is critical for full virulence of M. catarrhalis in the respiratory tract in facilitating

198 ignificantly contributes to the virulence of M. catarrhalis.

199 nhances membrane attack complex formation on M. catarrhalis and thus leads to increased serum sensiti

200 alpha1-4Galbeta1-4Glc) P(k) epitope found on M. catarrhalis 2951.

201 We focused our study on M. catarrhalis and found that PRELP binds the majority o

202 hances phagocytic killing of serum-opsonized M. catarrhalis by human neutrophils in vitro.

203 ed with S. pneumoniae, H. influenzae, and/or M. catarrhalis at 4 weeks of age.

204 zation with S. pneumoniae, H. influenzae, or M. catarrhalis is associated with increased risk of pneu

205 d to be present in the uspA2H genes of other M. catarrhalis strains.

206 f the uspA1 and uspA2 genes from three other M. catarrhalis strains (TTA24, ATCC 25238, and V1171) re

207 Immunoinformatics tools were used to predict M. catarrhalis epitopes that could offer immunoprotectio

208 the passenger domain from another predicted M. catarrhalis autotransporter confirmed the translocati

209 at immunoglobulin A (IgA) is the predominant M. catarrhalis-specific immunoglobulin isotype and that

210 A vaccine to prevent M. catarrhalis infections would have an enormous global

211 sing UspA1 and UspA2 in a vaccine to prevent M. catarrhalis infections.

212 oups would benefit from a vaccine to prevent M. catarrhalis infections.

213 human lung epithelial cells, thus protecting M. catarrhalis from intracellular killing by epithelial

214 The use of mutant and recombinant M. catarrhalis strains confirmed that the ORF113 protein

215 ion will likely decrease acute and recurrent M. catarrhalis infections in prone populations.

216 e entire uspA2 gene from the serum-resistant M. catarrhalis strain O35E resulted in a serum-sensitive

217 deposition on four different serum-resistant M. catarrhalis strains and their serum-sensitive uspA2 m

218 from the uspA2 genes in the serum-resistant M. catarrhalis strains O35E and O12E resulted in a drast

219 Testing of 11 additional serum-resistant M. catarrhalis wild-type isolates and their uspA1 and us

220 with the uspA2 gene from the serum-sensitive M. catarrhalis strain MC317.

221 The sequenced M. catarrhalis hemagglutinin-like locus of strain 7169 h

222 te vaccines derived from individual serotype M. catarrhalis only showed partial protection coverage.

223 prepared from individual colonies of several M. catarrhalis wild-type strains were analyzed by Wester

224 sequence analysis of the mapA gene from six M. catarrhalis strains showed that this protein was high

225 In this study M. catarrhalis fur has been cloned and sequenced from st

226 g KDO glycosylation is sufficient to sustain M. catarrhalis survival in vitro.

227 lator NsrR under aerobic conditions and that M. catarrhalis O35E nsrR mutants are unable to grow in t

228 gs in sham-inoculated animals confirmed that M. catarrhalis was exposed to significant host-derived f

229 Additionally, our studies demonstrate that M. catarrhalis cells form a mature biofilm in continuous

230 ter constructs were used to demonstrate that M. catarrhalis isolates with 10 G residues in their uspA

231 In this report we have demonstrated that M. catarrhalis can also utilize hemin as a sole source o

232 We recently demonstrated that M. catarrhalis cells that express the nitrite reductase

233 Previous work has demonstrated that M. catarrhalis expresses iron-repressible proteins, sugg

234 We have previously demonstrated that M. catarrhalis expresses specific outer membrane protein

235 three isogenic pil mutants demonstrated that M. catarrhalis expresses type IV pili that are essential

236 This study demonstrates that M. catarrhalis SodA plays a critical role in the detoxif

237 In this study, we discovered that M. catarrhalis expresses a cardiolipin synthase (CLS), t

238 We have found that M. catarrhalis can dramatically increase S. pyogenes adh

239 This novel observation indicates that M. catarrhalis strains lacking SodA constitutively expre

240 our findings uncover a novel mechanism that M. catarrhalis uses to evade host innate immunity.

241 In this study, we show that M. catarrhalis binds factor H via the outer membrane pro

242 The M. catarrhalis genome encodes a predicted truncated deni

243 The M. catarrhalis uspA1, uspA2, and uspA2H genes were clone

244 previously shown that expression of both the M. catarrhalis aniA (encoding a nitrite reductase) and n

245 plement regulator C4b-binding protein by the M. catarrhalis strains used in this study was found to b

246 We identified and cloned the M. catarrhalis genes encoding PilA, the major pilin subu

247 identified three open reading frames in the M. catarrhalis genome that encode homologues of the two-

248 A mutation in the M. catarrhalis hfq gene affected both the growth rate of

249 m a gene whose expression was altered in the M. catarrhalis hfq mutant.

250 an antibiotic resistance cartridge into the M. catarrhalis uspA2 gene resulted in the conversion of

251 quence analysis suggested that OMP B1 is the M. catarrhalis homologue to the transferrin binding prot

252 se sites are methylated in the genome of the M. catarrhalis 25239 ModM2 on strain.

253 The N-terminal portion of the M. catarrhalis acid phosphatase A (MapA) was most simila

254 ich normally extends from the surface of the M. catarrhalis cell.

255 experiments showed that introduction of the M. catarrhalis ETSU-9 uspA2H gene into Escherichia coli

256 The C-terminal half of the M. catarrhalis Hfq protein was very hydrophilic and cont

257 Cloning and expression of the M. catarrhalis mapA gene in Escherichia coli confirmed t

258 e specifying the putative transporter of the M. catarrhalis wild-type strains O35E, O12E, and McGHS1

259 The presence of the Hag protein on the M. catarrhalis cell surface, as well as that of the UspA

260 These findings suggest that the M. catarrhalis Hag protein is an adhesin for cell lines

261 arrhalis serotype A LOS, indicating that the M. catarrhalis LOS-directed antibody may play a major ro

262 These data suggest that the M. catarrhalis TbpA is necessary for the acquisition of

263 microscopy experiments demonstrated that the M. catarrhalis wild-type isolates O35E, O12E, TTA37, V11

264 Therefore, M. catarrhalis lgt3 was introduced into a defined beta(1

265 egative effect on biofilm formation by these M. catarrhalis strains in the crystal violet-based assay

266 f nasopharyngeal tissues isolated from these M. catarrhalis-infected animals revealed the presence of

267 This M. catarrhalis hfq mutant exhibited altered expression o

268 oretic mobility shift assay showed that this M. catarrhalis Hfq protein could bind RNA derived from a

269 y sevenfold, thereby demonstrating that this M. catarrhalis TPS system directly mediates binding to h

270 nce to human cells, the hag genes from three M. catarrhalis isolates were cloned and expressed in a n

271 Two of the other three M. catarrhalis ETSU-9 transposon insertion mutants that

272 s, and immunoassays to measure antibodies to M. catarrhalis.

273 known about the mucosal antibody response to M. catarrhalis in adults with COPD.

274 understanding the mucosal immune response to M. catarrhalis in the setting of COPD and in elucidating

275 is known about the human immune response to M. catarrhalis infection in vivo.

276 ili in the pathogenesis and host response to M. catarrhalis infections are warranted.

277 ilA in the pathogenesis and host response to M. catarrhalis infections are warranted.

278 erstanding of the humoral immune response to M. catarrhalis LOS epitopes developed during natural inf

279 develop variable humoral immune responses to M. catarrhalis after exacerbations, including new serum

280 s in understanding human immune responses to M. catarrhalis and in elucidating the elements of a prot

281 Adults with COPD make antibody responses to M. catarrhalis following infection, but little is known

282 Wild-type M. catarrhalis 2951 is resistant to complement-mediated

283 studies comparing the abilities of wild-type M. catarrhalis and an isogenic TFP mutant to colonize th

284 Provision of the wild-type M. catarrhalis hfq gene in trans eliminated these phenot

285 The presence of the wild-type M. catarrhalis hfq gene in trans in an E. coli hfq mutan

286 Wild-type M. catarrhalis strain O35E possessed a dense layer of su

287 The wild-type M. catarrhalis strains that formed the most extensive bi

288 No growth of wild-type M. catarrhalis was observed in minimal medium in which a

289 Twenty-four hours after inoculation, viable M. catarrhalis organisms were recovered from the nasal c

290 When M. catarrhalis is grown in the presence of hemin, HumA e

291 The genes were transcribed when M. catarrhalis was grown in vitro.

292 the best 3 peptides and then challenged with M. catarrhalis in the pulmonary clearance model.

293 ls in Ribi adjuvant and then challenged with M. catarrhalis strain 25238 or O35E or NTHi strain 12.

294 Colonization with M. catarrhalis and H. influenzae induced a mixed T helpe

295 Colonization of the hypopharynx with M. catarrhalis, S. pneumoniae, H. influenzae, and Staphy

296 rom children with otitis media infected with M. catarrhalis, antibody levels against peptide A were s

297 s, are expressed during human infection with M. catarrhalis, and represent potential vaccine antigens

298 following an aerosol challenge of mice with M. catarrhalis.

299 llowing intratracheal challenge of mice with M. catarrhalis.

300 MAb 8E7 reacted with M. catarrhalis serotype A and C LOSs but not serotype B