ライフサイエンスコーパス: MGP

1 MGP binds calcium ions through gamma-carboxylated glutam

2 MGP carboxylation status was not determined.

3 MGP colocalized intracellularly with BMP2.

4 MGP has also been identified as an inhibitor of bone mor

5 MGP has been shown to be an inhibitor of arterial wall a

6 MGP inhibited BMP-4 activity similarly to that of BMP-2

7 MGP inhibits calcification independent of BMP-2-driven o

8 MGP maps to chromosome 12p near D12S363.

9 MGP mRNA was assayed by relative quantitative and real-t

10 MGP overexpression reduced vascular BMP activity, athero

11 MGP promoter activity was also stimulated by BMP-4 in a

12 MGP protein is active and functions as an inhibitor of B

13 MGP requires to be activated by gamma-glutamyl carboxyla

14 MGP was a candidate on the basis of its localization to

15 MGP was also released into the medium and removed by ult

16 MGP/BMP2 colocalization was analyzed by confocal microsc

17 metallointercalator complex 1-Rh(MGP)2phi5+ [MGP = 4-(guanidylmethyl)-1,10-phenanthroline; phi = phen

18 BMP-4, MGP, ALK1, and ALK2 were predominantly expressed on the

19 y the cytomegalovirus (CMV; control, n = 6), MGP (n = 6), or VE-cad (n = 12) promoters.

20 agulation factor carboxylation and abolished MGP carboxylation at the physiological concentration of

21 vus and 20 E. gallinarum isolates) acidified MGP, 41 of 46 (89%) were LM and ARA positive, and 45 of

22 hanism represented by the presence of active MGP appears to be compromised in glaucomatous tissue.

23 Mice deficient in MGP alone (MGP(-/-) OPN(+/+)) showed calcification of their arterie

24 trate, methyl-alpha-D-glucopyranoside (alpha-MGP), stimulated release, whereas the SGLT1 inhibitor ph

25 ed larger GLP-1 responses than luminal alpha-MGP in matched concentrations.

26 zin (luminally) abolished responses to alpha-MGP and glucose.

27 n factor carboxylation but do not ameliorate MGP carboxylation.

28 we discuss how this can be implemented in an MGP framework and illustrate its application to simple m

29 years ago from the duplication of an ancient MGP gene and may exhibit intermediate functional feature

30 is expressed in endothelial cells (EC), and MGP deficiency results in developmental defects suggesti

31 proteins (a chimeric coagulation factor and MGP) in HEK293 cells.

32 fication inhibitory activities of fetuin and MGP may be related to their ability to form stable compl

33 ain structure to fetuin and, like fetuin and MGP, contains several residues of phosphoserine and accu

34 ions suggest that spp24 may, like fetuin and MGP, play a role in inhibiting calcification.

35 inhibited BMP-dependent neuronal growth and MGP expression increased in sympathetic neurons during t

36 n tumor angiogenesis, and identifies ID4 and MGP as possible therapeutic targets for GBM.

37 mm), phosphate by 1.6-fold (to 5.6 mm), and MGP by 25-fold (to 12 microg/ml).

38 fication and characterization of both OC and MGP from the Adriatic sturgeon, a ray-finned fish charac

39 OC, ON, and MGP were present in the deposits and vascular endothelia

40 Expression of VEGF and MGP was induced by TGF-beta1, but the induction of MGP p

41 tain MGP, was found to be recognized by anti-MGP antibodies.

42 TDC5 cells with inducible sense or antisense MGP cDNA constructs, we found that overexpression of MGP

43 3/4, AnkG, and MUC1 but not TM markers AQP1, MGP, CHI3L1, or TIMP3.

44 had twice as much arterial calcification as MGP(-/-) OPN(+/+) at 2 wk, and over 3 times as much at 4

45 Because MGP protein has been shown to play a key role in inhibit

46 To detect binding between MGP and BMP-2, we performed immunoprecipitation using MG

47 linking, and blocked the interaction between MGP and BMP-4.

48 The interaction between MGP and HSP70 was confirmed by coimmunoprecipitation and

49 HSP70 binds MGP and enhances BMP activity, thereby functioning as a

50 Importantly, mice deficient in both MGP and OPN had twice as much arterial calcification as

51 g for negative feedback regulation of BMP by MGP.

52 rrected the craniofacial anomalies caused by MGP deficiency, suggesting a local role for MGP in the d

53 identified by Luminex (for types detected by MGP PCR) or direct sequencing or cloning before sequenci

54 elial-epithelial interactions, maintained by MGP, are essential in pulmonary cell differentiation.

55 n of the calcification mechanism mediated by MGP could be used to regulate resistance and elevated IO

56 mRNAs were not detected in either calcified MGP(-/-) or noncalcified wild-type (MGP(+/+)) vessels.

57 uncarboxylated MGP (ucMGP) and carboxylated MGP (cMGP) than controls.

58 truct, which produced non-gamma-carboxylated MGP and fully gamma-carboxylated MGP.

59 arboxylated MGP and fully gamma-carboxylated MGP.

60 ssociate with reduced levels of carboxylated MGP, and inhibitory effects of quercetin do not involve

61 n, shown by mass spectrometry not to contain MGP, was found to be recognized by anti-MGP antibodies.

62 Conversely, MGP deficiency increased BMP activity, which may explain

63 arboxylase activity and were able to convert MGP to its active conformation.

64 ood vessels of matrix Gla protein deficient (MGP(-/-)) mice.

65 Expression of the gene encoding MGP was reduced in the glaucomatous tissue by -4.4 +/- 1

66 These experiments establish MGP as a novel regulator of BMP function in the nervous

67 in, the differentiating chondrocytes express MGP in a stage-specific biphasic manner as in vivo.

68 This highly specific complex of fetuin, MGP, and mineral prevents the growth, aggregation, and p

69 ybridization demonstrated that the genes for MGP and Ank were expressed locally in vibrissae, whereas

70 MGP or an intracellular carrier protein for MGP.

71 accumulation overlap with those reported for MGP; OC was detected in bone cells and mineralized struc

72 MGP deficiency, suggesting a local role for MGP in the developing nasal septum.

73 MGP dictates different transport routes for MGP in VSMCs.

74 The main advantages that follow from MGPs approach include the natural non-parametric represe

75 All three mutations predict a non-functional MGP.

76 The matrix GLA (MGP) gene has been found to be among the 10 most highly

77 g the inhibitor of calcification matrix Gla (MGP) in the trabecular meshwork cells.

78 When methyl-alpha-D-glucopyranoside (MGP) molecules are reacted with hexamethylene diisocyana

79 ification of methyl-alpha-D-glucopyranoside (MGP), and rapid motility (RM), for differentiating isola

80 We investigated how MGP carboxylation influences the risk of calciphylaxis i

81 for atherosclerosis, levels of BMP-4, HSP70, MGP, and interleukin-6 were elevated in the aortic wall.

82 Human MGP is a 10-kD skeletal extracellular matrix (ECM) prote

83 c EC with increasing concentrations of human MGP (hMGP) for 24 h.

84 orphisms in the promoter region of the human MGP gene.

85 ndem mass spectrometric analysis to identify MGP-binding proteins.

86 In MGP a subpopulation of neurons exhibiting low NR2D signa

87 ion, and the development of cerebral AVMs in MGP null (Mgp(-/-)) mice.

88 ults suggest that BMP and calcium binding in MGP are independent but functionally intertwined process

89 The importance of elastin calcification in MGP null vascular disease is highlighted by significant

90 se calcification of vascular medial cells in MGP deficient aortas and the increase in expression of a

91 osclerotic lesion formation was decreased in MGP-deficient mice, which may be explained by a dramatic

92 Mice deficient in MGP alone (MGP(-/-) OPN(+/+)) showed calcification of th

93 Our data indicate that mutations in MGP are responsible for KS and confirm its role in the r

94 ontains structural features usually found in MGPs (e.g. a putative phosphorylated propeptide).

95 Increased MGP expression inhibited BMP-dependent neuronal growth a

96 n effect that may be limited by ALK1-induced MGP.

97 tein the most gamma-carboxylated among known MGPs.

98 stribution and accumulation typical of known MGPs, and it contains seven possible Gla residues that w

99 lyethylene glycol (PEG) to form cross-linked MGP-polyurethane (PUR) networks, these materials are cap

100 Mechanistically, MGP deficiency increased BMP activity in lungs.

101 a vitamin K-dependent enzyme, which mediates MGP carboxylation.

102 In the mouse model, MGP null vascular disease presents as calcifying cartila

103 content, increased ALP, decreased normalized MGP expression and lower gamma-carboxylase activity.

104 soluble in serum, was identified as a novel MGP-binding protein.

105 further studied in matrix GLA protein null (MGP(-/-)) mice whose arteries spontaneously calcify.

106 treatment of cells led to loss of ability of MGP to bind BMP-4.

107 The ability of MGP to inhibit calcification requires the activity of a

108 Acidification of MGP was therefore the single most useful test for differ

109 As a result, activation of MGP by gamma-glutamyl carboxylase is diminished, allowin

110 Mutational analysis of MGP in three unrelated probands identified three differe

111 ynthesis and that the gamma-carboxylation of MGP is necessary for its binding to the serum mineral co

112 Aortic content of MGP mRNA was increased 5-fold in renal failure but did n

113 The effect of MGP mutagenesis on vascular calcification was determined

114 This study showed that the effect of MGP on ALK1 signaling and VEGF expression in bovine aort

115 To quantify the effect of MGP on BMP-2 activity, we assayed for alkaline phosphata

116 ed VEGF expression, we studied the effect of MGP on the activity of transforming growth factor (TGF)-

117 antibodies to TGF-beta blocked the effect of MGP on VEGF expression.

118 tion of ALK1 signaling induced expression of MGP in addition to that of VEGF, allowing for negative f

119 onal, more acidic Ser-phosphorylated form of MGP believed to be the product of Golgi casein kinase.

120 Both forms of MGP were found in the cytosolic and microsomal fractions

121 eriments show that the anti-ECMM function of MGP requires four amino acids which are gamma-carboxylat

122 ) does not display the anti-ECMM function of MGP.

123 se-dependent, that a progressive increase of MGP levels ceased to be stimulatory and instead turned i

124 vels on ALK1 expression and the induction of MGP and VEGF.

125 l interfering RNA abolished the induction of MGP and VEGF.

126 cts of quercetin do not involve induction of MGP carboxylation.

127 s induced by TGF-beta1, but the induction of MGP preceded that of VEGF, consistent with a promoting e

128 Inhibition of MGP resulted in smaller and less vascularized xenografts

129 Because lack of MGP also causes arterial calcification, our findings dem

130 Increased BMP activity due to the lack of MGP induces expression of the activin receptor-like kina

131 In contrast, raising the serum level of MGP does not affect mineralization of any ECM.

132 High levels of MGP (>15-fold excess), however, resulted in pronounced e

133 ing studies showed that inhibitory levels of MGP abolished BMP-2 receptor binding.

134 his work suggests that coordinated levels of MGP are required for chondrocyte differentiation and mat

135 Low levels of MGP relative to BMP-2 (<1-fold excess) resulted in mild

136 Enhancing levels of MGP resulted in increased Smad1 activation.

137 Inhibitory levels of MGP yielded increased matrix binding of BMP-2, suggestin

138 ependent gamma-carboxylation modification of MGP.

139 However, overexpression of MGP during the hypertrophic phase has no effect on chond

140 Overexpression of MGP in HTM cells reduced ALP activity in a model of BMP2

141 constructs, we found that overexpression of MGP in maturing chondrocytes and underexpression of MGP

142 The data suggest that phosphorylation of MGP dictates different transport routes for MGP in VSMCs

143 ed to identify a larger soluble precursor of MGP or an intracellular carrier protein for MGP.

144 lar localization of BMP-2 in the presence of MGP, binding assays were performed on whole cells and ce

145 Further, down-regulation of MGP by shRNA does not alter the effect of quercetin.

146 To determine the role of MGP in EC, we cultured bovine aortic EC with increasing

147 e of this study was to determine the role of MGP in the vasculature of the lungs and kidneys.

148 ct on ALK1 expression and the stimulation of MGP and VEGF expression were dependent on signaling by t

149 Studies of MGP-deficient mice suggest that MGP is an inhibitor of e

150 Synthesis of MGP is increased in renal failure and deficiency of GlaM

151 on intracellular processing and transport of MGP to become an extracellular binding protein for bone

152 maturing chondrocytes and underexpression of MGP in proliferative and hypertrophic chondrocytes induc

153 mined the effects of vitamin K deficiency on MGP carboxylation.

154 Only MGP proteins with preserved ability to bind and inhibit

155 We used MGP transgenic or MGP-deficient mice bred to apolipoprotein E mice, a mode

156 pallidus (LGP), and medial globus pallidus (MGP).

157 ation) from a former manufactured gas plant (MGP) site was treated in a laboratory scale bioreactor (

158 Unlike plants, MGP-PUR networks require no photo-initiated reactions, a

159 In the mammalian growth plate, MGP is expressed by proliferative and late hypertrophic

160 methyl-D-glucose-containing polysaccharides (MGPs), synthetic 6-O-methyl-D-glucose-containing polysac

161 ain reactions with modified general primers (MGP) and Forslund-Antonsson primers (FAP) and identified

162 based on multiple-output Gaussian processes (MGPs), which are a flexible non-parametric Bayesian mode

163 Matrix gamma-carboxyglutamic acid protein (MGP) is a member of the vitamin K-dependent protein fami

164 Matrix gamma-carboxyglutamic acid protein (MGP) is a mineral-binding extracellular matrix protein s

165 ts of matrix gamma-carboxyglutamate protein (MGP).

166 is and is antagonized by matrix Gla protein (MGP) and crossveinless 2 (CV2), both induced by the acti

167 MP-4; the BMP inhibitors matrix Gla protein (MGP) and Noggin; activin-like kinase receptor (ALK)1, -2

168 Osteocalcin (OC) and matrix Gla protein (MGP) are considered evolutionarily related because they

169 sis of the properties of matrix Gla protein (MGP) as a vitamin K-dependent calcification inhibitor.

170 eral, 80% fetuin, and 2% matrix Gla protein (MGP) by weight, and the presence of the complex in serum

171 ing and/or inhibition of matrix Gla protein (MGP) carboxylation.

172 unction mutations in the matrix Gla protein (MGP) gene.

173 Matrix GLA protein (MGP) has been identified as a calcification inhibitor in

174 Matrix GLA protein (MGP) has previously been shown to enhance expression of

175 Mutations in matrix Gla protein (MGP) have been correlated with vascular calcification.

176 Matrix Gla protein (MGP) is a 14-kD extracellular matrix protein of the mine

177 Matrix Gla protein (MGP) is a potent inhibitor of vascular calcification.

178 Matrix Gla protein (MGP) is an antagonist of BMPs that is highly expressed i

179 Matrix Gla protein (MGP) is an inhibitor of vascular calcification but its m

180 Matrix GLA protein (MGP) is expressed in endothelial cells (EC), and MGP def

181 Matrix GLA protein (MGP) is ubiquitously expressed with high accumulation in

182 t mice were crossed with matrix Gla protein (MGP) mutant mice.

183 s and expressed elevated matrix GLA protein (MGP) that mediated enhanced tumor angiogenesis.

184 the proteins fetuin and matrix Gla protein (MGP) that was initially discovered in serum of rats trea

185 show that deficiency of matrix Gla protein (MGP), a BMP inhibitor, causes induction of Notch ligands

186 nduced the expression of matrix Gla protein (MGP), a regulator of BMP function in the vascular system

187 sulting from the loss of matrix Gla protein (MGP), causes ectopic hepatic differentiation in the pulm

188 ion in the expression of matrix Gla protein (MGP), osteopontin (OPN), and vascular calcification-asso

189 s, in part, prevented by matrix Gla protein (MGP).

190 ors osteonectin (ON) and matrix Gla protein (MGP).

191 to reduced fetuin-A and matrix gla-protein (MGP) levels.

192 itive immunostaining for matrix-gla-protein (MGP), fetuin-A, and ankylosis protein (Ank) as well as a

193 ent with binding models in which Lambda-1-Rh(MGP)2phi5+ (Lambda-Rh) traps the recognition site 5'-CAT

194 e symmetric metallointercalator complex 1-Rh(MGP)2phi5+ [MGP = 4-(guanidylmethyl)-1,10-phenanthroline

195 obility assays demonstrated that Lambda-1-Rh(MGP)2phi5+ at 120 nM competes 50% of yAP-1 binding to th

196 The metallointercalator Lambda-1-Rh(MGP)2phi5+ binds tightly and specifically to the site 5'

197 , the preferred binding site for Lambda-1-Rh(MGP)2phi5+ was engineered into the AP-1 recognition elem

198 , including geometric isomers of Lambda-1-Rh(MGP)2phi5+, show no specific binding to the target site

199 a high-affinity binding site for Lambda-1-Rh(MGP)2phi5+, whereas the native ARE showed no interaction

200 the native ARE requires 3 microM Lambda-1-Rh(MGP)2phi5+.

201 nist warfarin prevents the increase in serum MGP after etidronate injection, which shows that the inc

202 tion, which shows that the increase in serum MGP is due to new synthesis and that the gamma-carboxyla

203 ression were independent of changes in serum MGP.

204 138C) is significantly correlated with serum MGP levels in human subjects.

205 Silencing MGP by siRNA resulted in ALP activity that was increased

206 Since MGP is an insoluble matrix protein, this work has focuse

207 Sturgeon MGP shows a primary structure, post-translation modifica

208 othelial cells with N-terminally FLAG-tagged MGP and used immunoprecipitation and liquid chromatograp

209 significantly earlier (4.4 +/- 0.2 wk) than MGP(-/-) OPN(+/+) counterparts (6.6 +/- 1.0 wk).

210 The fact that MGP is present in the general circulation raises the que

211 on of Matrix gla protein (Mgp) revealed that MGP is an inhibitor of ECMM.

212 These results suggest that MGP is a BMP-2 regulatory protein.

213 Studies of MGP-deficient mice suggest that MGP is an inhibitor of extracellular matrix calcificatio

214 Together, these results suggest that MGP modulates BMP activity.

215 Together, the results suggest that MGP plays a role in EC function, altering the response t

216 nthesis inhibitor cyclohexamide suggest that MGP, OPN, and VCAF mRNA abundance are controlled at diff

217 sed matrix binding of BMP-2, suggesting that MGP inhibits BMP-2 in part via matrix association.

218 The MGP promoter fragment resulted in beta-galactosidase exp

219 Directed expression by the MGP gene promoter specifically to the trabecular meshwor

220 ere infected with an adenovirus carrying the MGP construct, which produced non-gamma-carboxylated MGP

221 e suggests that a common polymorphism of the MGP promoter influences binding of the AP-1 complex, whi

222 However, the fraction of total MGP that was carboxylated (relative cMGP concentration =

223 ting CAC, independent of its effect on total MGP concentrations.

224 alcified MGP(-/-) or noncalcified wild-type (MGP(+/+)) vessels.

225 concentration = cMGP/[cMGP + uncarboxylated MGP]) was lower in cases than in controls (0.58+/-0.02 v

226 s had higher plasma levels of uncarboxylated MGP (ucMGP) and carboxylated MGP (cMGP) than controls.

227 We used MGP transgenic or MGP-deficient mice bred to apolipoprot

228 MP-2, we performed immunoprecipitation using MGP and BMP-2 tagged with FLAG and c-Myc.

229 ification in cartilage and arterial vessels, MGP's function in human TM was investigated.

230 The objective was to determine whether MGP also binds other proteins, which could interfere wit

231 To test whether MGP affects BMP-induced differentiation, three sets of e

232 esults showed co-precipitation of BMP-2 with MGP.

233 ts in vascular calcification associated with MGP dysfunction and emphasize the need for a comprehensi

234 eries was greatly up-regulated compared with MGP wild-types.

235 Treatment of the ATDC5 cultures with MGP antiserum during the proliferative phase leads to th

236 estern blot analyses were cross-reacted with MGP N-terminal- and conformational-specific antibodies.

237 the Ins2(Akita/+) mice by breeding them with MGP transgenic mice, which increased aortic BMP inhibiti

238 without loss of function and that zebrafish MGP, which lacks upstream Gla residues, did not function