ライフサイエンスコーパス: MLST

1 MLST analysis demonstrated the presence of 35 different

2 MLST analysis indicated that the C. jejuni isolates util

3 MLST analysis of 15 representative isolates from differe

4 MLST analysis revealed no lineage specific differences b

5 MLST and PFGE demonstrated a capsular switching from CPS

6 MLST and plasmid analysis shows that MCRPEC are diversel

7 MLST assigned 80% of CR-Kp isolates to the ST258-clone.

8 MLST data revealed that all isolates harboring the major

9 MLST data suggested that there was a large amount of gen

10 MLST demonstrated that the majority (87.5%; 7/8) of C. d

11 MLST findings revealed that sequence type 5 (ST5) was th

12 MLST identified 24 sequence types (STs), of which 19 wer

13 MLST is used to generate allelic profiles to characteriz

14 MLST is useful for investigating the epidemiology, genet

15 MLST of 42 6C isolates revealed 12 genotypes distributed

16 MLST resolved 64 B. multivorans sequence types.

17 MLST results suggest 6C strains arose from independent r

18 MLST revealed a 1 in 3198 nucleotide difference between

19 MLST showed five clusters related to serotype 6A, two cl

20 MLST studies of selective isolates from the four protein

21 MLST was less discriminatory than either MLVA or REA, ye

22 MLST was used to assess the genetic diversity of 192 GBS

23 MLST, DNA microarrays, and genome sequencing has allowed

24 cleotide polymorphism (SNP) loci, forming 25 MLST subtypes.

25 y sequenced travel related isolates, and 312 MLST profiles.

26 Using 24 PHAT probes, all 62 MLST CGs in the representative E. coli collection were d

27 2 E. coli isolates, representing at least 62 MLST CGs and diverse disease states, using a "library-on

28 From 7343 MLST-characterised isolates, we sequenced 600 C. jejuni

29 environmental strains that had the same AFLP/MLST genotypes.

30 tween short sequence strings (k-mers) and an MLST allele library.

31 species level by use of kmer comparisons and MLST.

32 a more powerful typing method than MLVA and MLST combined (D = 0.67).

33 The PFGE, MLVA, and MLST profiles were consistent with the predominate types

34 h genetic diversity was revealed by PFGE and MLST.

35 truly nontypeable by Quellung reaction, and MLST and the presence of psaA proved useful in distingui

36 on common epidemiological surrogates such as MLST.

37 catenated locus sequences within and between MLST clusters confirmed the seven previously named Achro

38 By MLST, all isolates belonged to the international clone I

39 solates, belong to sequence type 8 (ST-8) by MLST and serotype HS:1,8, further indicating the clonali

40 effect of genetic background (as assessed by MLST) over and above capsule.

41 ical but where the two sets differed both by MLST and ABC typing.

42 ention that were previously characterized by MLST and pulsed-field gel electrophoresis (PFGE).

43 . acnes isolates previously characterized by MLST and representing types IA1 (n=145), IA2 (n=20), IB

44 PS type IV and thus further characterized by MLST typing, pulsed-field gel electrophoresis (PFGE), an

45 A. baumannii clonal lineages (as defined by MLST) circulated during the study, three of which are gl

46 C. acnes clinical isolates was determined by MLST.

47 t this clade that cannot be distinguished by MLST.

48 ging to major epidemic lineages, followed by MLST analysis to categorize isolates from common lineage

49 ates of an outbreak strain as those found by MLST and PFGE.

50 riants (differing by a single nucleotide) by MLST and were therefore also classified as clonally rela

51 y positive and clustered with pneumococci by MLST (2 were bile soluble); 8 lacked psaA (5 ply positiv

52 cidence and molecular epidemiology of PPE by MLST in Utah children after the licensure of PCV-7.

53 uced results comparable to those produced by MLST for the identification of the major epidemic lineag

54 ccal sequence types previously recognized by MLST.

55 efore also classified as clonally related by MLST.

56 At least two major ecotypes, represented by MLST clades A and E, were proposed based on genetic, eco

57 existence of seven species was supported by MLST.

58 ifferences in ABC type for tightly clustered MLST types and intermittent appearances of MTL homozygos

59 PHAT classification to the whole collection, MLST validation of the PHAT probe classification resulte

60 The most common MLST genotypes among the isolates were the dominant glob

61 variants were detected in each of the common MLST clonal complexes.

62 By comparison, MLST identified 60 sequence types that could be clustere

63 the housekeeping genes used by conventional MLST strategies.

64 ng its enhanced resolution over conventional MLST analysis.

65 The current MLST scheme uses sequences of 7 genes to generate an ST,

66 Of the 19 different MLST subgroups, 16 corresponded to 16 distinct ETST grou

67 een 6C isolates shared one of four different MLST types with 6C-negative CS6As.

68 Moreover, this discriminatory MLST scheme retains the ability to identify epidemiologi

69 of 3042 isolates, representing 696 distinct MLST genotypes, from a well-established database (www.ml

70 om six countries, with our newly established MLST scheme identified a total of 20 sequence types (STs

71 This facilitated an expanded MLST approach utilizing large numbers of loci for isolat

72 We propose that an extended MLST scheme with approximately 50 genes provides optimal

73 rcentages of isolates with complete extended MLST profiles ranged from 99.1% (50 genes) to 86.8% (1,4

74 lymorphism (SNP)-based method, (ii) extended MLST using different numbers of genes, (iii) determinati

75 From MLST, no more than 25% of cases could be linked to a pot

76 When compared with the nine gene MLST scheme developed at the University of Bath, UK, and

77 types (ETST), while the standard seven-gene MLST profiles differentiated the same 61 isolates into 1

78 Genetic MLST clustering was confirmed by genomic sequence analys

79 trace back investigations, with core genome MLST (cgMLST) analysis as one of the most straightforwar

80 A core genome MLST (cgMLST) scheme defines a comprehensive set of thos

81 Whole-genome MLST effectively distinguished between highly similar ou

82 distinctions between strains with identical MLST profiles and showed a discriminatory power similar

83 vestigated belonged to previously identified MLST complex 2, where most isolates from patients cluste

84 st all (97%) of the R variants were found in MLST clonal complex 8 (CC8), while the H variant was bro

85 gpi genes are not good candidates for use in MLST analysis and that a SBT-bla(OXA-51-like) gene schem

86 al to expand to more loci than those used in MLST and even to other bacterial species.

87 ied as separate STs by the Pasteur Institute MLST system, and an ST131 PCR method that targets the O2

88 All major MLST complexes include strains in both subfamily A and s

89 Using the scheme PFGE-MLVA-MLST-prn mutations-Prn deficiency, the 240 isolates comp

90 From this database, one or more MLST sequence types (STs) that comprised a PSGS genotype

91 In addition, a new MLST profile (ST1071) was observed in South Africa.

92 , although three novel spa types and a novel MLST (ST1518) were detected.

93 Here, we describe the development of a novel MLST system to assist with the investigation of an unusu

94 The data obtained by analysis of MLST and SBT-bla(OXA-51-like) genes were compared to the

95 ble to that of spa typing (D = 0.445) and of MLST (D = 0.417) in the first collection.

96 prospect of increasingly wide application of MLST.

97 his study demonstrates that a combination of MLST and MLVA may prove useful for the investigation and

98 manner similar to major clonal complexes of MLST, indicating coevolution between the chromosomal bac

99 tes, one had a composite genotype profile of MLST ST 5-PFGE USA100-unknown spa type, which has been r

100 se compositions of 65- to 100-bp sections of MLST alleles compiled within http://www.mlst.net.

101 f discrimination similar in value to that of MLST (0.924 and 0.953 compared to 0.96), but discriminat

102 The utility of MLST in regard to cystic fibrosis (CF)-related infection

103 d using the asymmetric island model based on MLST data of human and animal/food isolates.

104 e investigated M. bovis population, based on MLST data.

105 mean ISS for each S. aureus clone (based on MLST) was compared with its DNA microarray-based genotyp

106 Based on MLST, we selected several B. anthracis, B. cereus, and B

107 pa type 539/t034 that were of ST398 based on MLST.

108 Forty-one MLST sequence types (STs) were observed.

109 (CCs) were assigned based on the spa and/or MLST results.

110 We performed MLST with 65 of the 334 surveillance isolates (61 S. dys

111 Using PFGE, MLST, and spa typing, three retail beef MRSA isolates we

112 led a limited diversity in surface proteins, MLST types, and DNA macrorestriction profiles for type I

113 reference sequences available in the public MLST database.

114 corresponding to the Streptococcus pyogenes MLST scheme.

115 relatedness of the 46 strains recapitulated MLST types and provided greater interstrain differentiat

116 hromosome transfer of vancomycin resistance, MLST markers, and capsule genes as well.

117 f discrimination range from 0.972 (ribosomal MLST) to 0.999 (SNP based), and all values were higher t

118 er: MLVA, REA, PFGE, slpAST, PCR-ribotyping, MLST, and AFLP.

119 d gel electrophoresis) patterns and the same MLST (multilocus sequence typing) sequence type (ST-1068

120 We also used the same MLST system to perform a retrospective analysis on isola

121 scribed a multilocus sequence typing scheme (MLST) for P. acnes based on seven housekeeping genes.

122 the first multilocus sequence typing scheme (MLST) for P. larvae, which largely confirms the previous

123 Seven MLST types were identified, with the majority of the iso

124 ausing different invasive infections, shared MLST complexes and exhibited identical or closely relate

125 sent a coalescent method to jointly simulate MLST data and the clonal genealogy that gave rise to the

126 All the isolates belonged to a single MLST type, sequence type ST19.

127 isolates, respectively) represented a single MLST type.

128 REA, slpAST, MLST, and PCR-ribotyping all included AP-B (toxinotype I

129 he isolates recovered from horses, a smaller MLST and MLSA defined sub-population seems to be able to

130 ng MALDI-TOF MS fingerprints of the standard MLST loci to reference sequences available in the public

131 GE had a greater discriminatory ability than MLST for P. aeruginosa isolates (D values, 0.999 versus

132 mined that PFGE was more discriminatory than MLST for determining genetic differences in P. aeruginos

133 PCR was found to be more discriminatory than MLST/staphylococcal cassette chromosome mec (SCCmec) typ

134 We conclude that MLST and core genome SNP typing result in the same phylo

135 ions, and repertoires, and MDS revealed that MLST genotypes were strongly associated with particular

136 The MLST loci used in this scheme were shown to be stable in

137 The MLST scheme uses genes with an appropriate clock speed a

138 ory and demonstrated relationships among the MLST genetic lineages and REA genotypes that were previo

139 re appeared to be an association between the MLST types and protein expression profiles.

140 olates, while the second isolate carried the MLST ST 8-PFGE USA300-spa type t121 genotype, commonly i

141 However, the MLST data were not always in concordance with the PFGE d

142 Importantly, the MLST data suggest that B. burgdorferi originated in Euro

143 Despite the low level of diversity in the MLST loci, a neighbor net analysis revealed a variable r

144 phical segregation were both observed in the MLST subtypes.

145 ased on the relatedness of the isolates, the MLST data supported the hypothesis that infections in th

146 The molecular clock speed of the MLST genes was studied using 219 colonies isolated longi

147 eby providing the basis for extension of the MLST scheme, which is currently restricted to C. diphthe

148 Overall, the MLST and MLVA data were concordant with REA genotyping d

149 By adapting several primer sequences, the MLST genes in C. ulcerans were also amplified, thereby p

150 nomic sequence analysis, indicating that the MLST scheme developed in this study is representative of

151 cies and were resolved with reference to the MLST data.

152 d 24 sequence types (STs); 9 were new to the MLST database.

153 We validated the MLST scheme on B. burgdorferi specimens from North Ameri

154 ride different from that associated with the MLST lineage were considered to demonstrate capsular swi

155 on PFGE were largely in concordance with the MLST results, with a similar amount of genetic diversity

156 At the time of writing, over thirty MLST schemes have been published and made available on t

157 This MLST scheme was applied to 100 V. parahaemolyticus strai

158 This MLST system showed high intraspecies discriminatory powe

159 Furthermore, this MLST scheme was shown to be more discriminatory than bot

160 Application of this MLST scheme to more V. parahaemolyticus strains and by d

161 Using this MLST scheme, we analyzed 107 genetically diverse Achromo

162 According to MLST, all isolates belonged to sequence type 36.

163 s more closely related to VGIII according to MLST.

164 al setting and may provide an alternative to MLST for discriminating isolates.

165 ococcal Opa repertoire is strongly linked to MLST genotype irrespective of epidemiological sampling a

166 ates of Fusarium collected were subjected to MLST to identify the phylogenetic species and sequence t

167 This confirms that WGS is superior to MLST for evolutionary analyses and is more accurate than

168 plexes, belongs to multilocus sequence type (MLST) 10, and is most closely related to strains isolate

169 moniae serotype 1, multilocus sequence type (MLST) 227.

170 palindromic-PCR), multilocus sequence type (MLST) analysis, and WGS on 148 Acinetobacter calcoacetic

171 correlated with a multilocus sequence type (MLST) clonal complex associated with specific PVL phage

172 ferent serotypes, multilocus sequence types (MLST), and sites of clinical isolation.

173 ylogeny, identify multilocus sequence types (MLST), multiantigen sequence types (NG-MAST), and molecu

174 geny and identify multilocus sequence types (MLST), N. gonorrhoeae multiantigen sequence types (NG-MA

175 types and MCRPEC multi-locus sequence types (MLSTs) were more variable in Guangdong than in Zhejiang

176 nce between PFGE, multilocus sequence types (MLSTs), and rtxA gene sequencing results was also examin

177 s representing 24 multilocus sequence types (MLSTs), including human commensal and clinical isolates

178 3 spa types and 2 multilocus sequence types (MLSTs).

179 ntries presented multilocus sequence typing (MLST) alleles, sequence types and emm types (only 56% of

180 performed using multilocus sequence typing (MLST) alongside traditional phenotypic methods.

181 Multilocus sequence typing (MLST) analysis was performed on a subset of the strains.

182 erent sources by multilocus sequence typing (MLST) and ABC typing of rRNA genes and determined their

183 anine sources by multilocus sequence typing (MLST) and examined their profile of putative adhesin-enc

184 ty inferred from multilocus sequence typing (MLST) and genome-wide SNP-based phylogenetic assays were

185 Multilocus sequence typing (MLST) and multi-virulence-locus sequence typing (MVLST)

186 In this study, multilocus sequence typing (MLST) and multilocus variable-number tandem-repeat analy

187 arly typed using multilocus sequence typing (MLST) and multispacer sequence typing (MST).

188 characterized by multilocus sequence typing (MLST) and outer membrane protein gene sequencing.

189 genotyped using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE).

190 equaled that of multilocus sequence typing (MLST) and pulsed-field gel electrophoresis.

191 were analyzed by multilocus sequence typing (MLST) and sequence analysis of virulence-associated gene

192 MSSA isolates by multilocus sequence typing (MLST) and spa typing in this study showed a genetic simi

193 solates included multilocus sequence typing (MLST) and staphylococcal protein A gene (spa) typing res

194 gene sequencing, multilocus sequence typing (MLST) and whole genome clustering of data from comparati

195 pared to that of multilocus sequence typing (MLST) and whole-genome optical maps.

196 newly developed multilocus sequence typing (MLST) approach and elucidates the diversity, distributio

197 , we have used a multilocus sequence typing (MLST) approach employing the alleles of 7 genes (atpD, f

198 yping, and rapid multilocus sequence typing (MLST) by electrospray ionization mass spectrometry of PC

199 roups similar to multilocus sequence typing (MLST) clonal groups (CGs) could be determined.

200 een CPS type and multilocus sequence typing (MLST) cluster, with the remarkable exception of the worl

201 Although multilocus sequence typing (MLST) currently represents the gold standard for unambig

202 Multilocus sequence typing (MLST) data demonstrated that livestock associated clonal

203 ructure based on multilocus sequence typing (MLST) data derived from the WGS data showed that the rem

204 the analysis of multilocus sequence typing (MLST) data, at http://mgip.biology.gatech.edu.

205 from the public multilocus sequence typing (MLST) database established a reference set of expected p

206 A multilocus sequence typing (MLST) database for V. parahaemolyticus was created in 20

207 Multilocus sequence typing (MLST) demonstrated 21 known and 2 novel sequence types (

208 resis (PFGE) and multilocus sequence typing (MLST) for 90 P. aeruginosa isolates obtained from cultur

209 repertoires and multilocus sequence typing (MLST) genotypes.

210 or studies using multilocus sequence typing (MLST) have found specific GBS clones (e.g., sequence typ

211 between WGS and multilocus sequence typing (MLST) identified major discrepancies for 17% of isolates

212 pacer region and multilocus sequence typing (MLST) indicated a predominant tetO-positive, doxycycline

213 Multilocus sequence typing (MLST) is a genetic typing tool designed to provide infor

214 Multi-locus sequence typing (MLST) is a widely used method of characterization of bac

215 Multilocus sequence typing (MLST) is the gold standard genotyping technique for many

216 ination in seven multilocus sequence typing (MLST) loci from 94 invasive, colonizing, and bovine stra

217 and 8 targeting multilocus sequence typing (MLST) loci were employed for each of 229 highly diverse

218 We performed multilocus sequence typing (MLST) on 590 pneumococcal isolates obtained during the A

219 We performed multilocus sequence typing (MLST) on a limited sampling of 6C isolates with differen

220 We performed multilocus sequence typing (MLST) on all isolates and sequenced fragments of the luk

221 We formulated multilocus sequence typing (MLST) primers with six of the seven loci corresponding t

222 Recent multilocus sequence typing (MLST) refined the relatedness of B. cereus group members

223 enes agreed with multilocus sequence typing (MLST) results for typing of 49 of the 50 isolates; in ad

224 Multilocus sequence typing (MLST) revealed 24 sequence types (STs); 9 were new to th

225 resis (PFGE) and multilocus sequence typing (MLST) revealed a high level of concordance in the cluste

226 Multilocus sequence typing (MLST) revealed sequence type 5 (ST5) (n = 2), ST6 (n = 3

227 ore, developed a multilocus sequence typing (MLST) scheme for B. burgdorferi based on eight chromosom

228 A multilocus sequence typing (MLST) scheme for M. pneumoniae was developed based on th

229 k, we describe a multilocus sequence typing (MLST) scheme for V. parahaemolyticus based on the intern

230 this pathogen, a multilocus sequence typing (MLST) scheme was developed and applied to the characteri

231 robustness of a multilocus sequence typing (MLST) scheme, based on conserved regions of seven housek

232 , we introduce a multilocus sequence typing (MLST) scheme, comprised of seven single-copy housekeepin

233 elop an expanded multilocus sequence typing (MLST) scheme.

234 ed a genus-level multilocus sequence typing (MLST) scheme.

235 s and subsequent multilocus sequence typing (MLST) separated a subset of 77 isolates into 24 sequence

236 Multi locus sequence typing (MLST) suggests presence of genetic homogeneity across wS

237 first extensive multilocus sequence typing (MLST) survey of plumbing drain-associated Fusarium isola

238 1 by the Achtman multilocus sequence typing (MLST) system and a screening PCR assay that targets ST13

239 esis (PFGE), and multilocus sequence typing (MLST) to characterize them and identify trends in DNA cl

240 Here, we used multilocus sequence typing (MLST) to compare the molecular genotypes of strains of C

241 Multilocus sequence typing (MLST) together with macrorestriction analysis of genomic

242 Multilocus sequence typing (MLST) was able to accurately predict the serovars for 42

243 Nested multilocus sequence typing (MLST) was employed for case specimen extracts.

244 Multilocus sequence typing (MLST) was performed on 107 B. multivorans isolates to pr

245 Multilocus sequence typing (MLST) was proposed in 1998 as a portable, universal, and

246 esis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality.

247 CR (rep-PCR) and multilocus sequence typing (MLST) were performed.

248 esis (PFGE), and multilocus sequence typing (MLST) were performed.

249 and psaA genes, multilocus sequence typing (MLST), 16S rRNA gene sequencing, and pyrosequencing.

250 Multilocus sequence typing (MLST), a sequence-based method to characterize bacterial

251 e type (ST) 8 by multilocus sequence typing (MLST), all of which are characteristics commonly attribu

252 phoresis (PFGE), multilocus sequence typing (MLST), and clustered regularly interspaced short palindr

253 electrophoresis, multilocus sequence typing (MLST), and molecular capsule typing (C-patterns and wzi

254 analysis (MLVA), multilocus sequence typing (MLST), and pertactin gene (prn) mutational analysis.

255 analysis (REA), multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE).

256 phoresis (PFGE), multilocus sequence typing (MLST), and serotyping.

257 A (spa) typing, multilocus sequence typing (MLST), and staphylococcal cassette chromosome mec (SCCme

258 A (spa) typing, multilocus sequence typing (MLST), and staphylococcal cassette chromosome mec elemen

259 phoresis (PFGE), multilocus sequence typing (MLST), and staphylococcal protein A (spa) typing.

260 olates underwent multilocus sequence typing (MLST), as well as assays for the Panton-Valentine leukoc

261 itive strains by multilocus sequence typing (MLST), but surprisingly, all strains investigated contai

262 roaches, such as multilocus sequence typing (MLST), by substantially increasing the number of loci ex

263 rable to that of multilocus sequence typing (MLST), is applicable to mixtures, and is highly automate

264 T), analogous to multilocus sequence typing (MLST), is the current "gold standard" typing method for

265 ently evaluated, multilocus sequence typing (MLST), often lacks discrimination and can be expensive.

266 nalyzed by using multilocus sequence typing (MLST), plasmid profiling, hybridization to a pan-Salmone

267 t Britain, using multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and arra

268 ibility testing, multilocus sequence typing (MLST), spa typing, SCCmec typing, and pulsed-field gel e

269 rmed as ST398 by multilocus sequence typing (MLST), which prompted retrospective analysis of all MRSA

270 n sequencing for multilocus sequence typing (MLST).

271 gerprinting, and multilocus sequence typing (MLST).

272 action (PCR) and multilocus sequence typing (MLST).

273 ere genotyped by multilocus sequence typing (MLST).

274 ere evaluated by multilocus sequence typing (MLST).

275 ages defined by multi-locus sequence typing (MLST).

276 ined by standard multilocus sequence typing (MLST).

277 s then underwent multilocus sequence typing (MLST).

278 esis (PFGE), and multilocus sequence typing (MLST).

279 characterized by multilocus sequence typing (MLST).

280 ion mapping, and multilocus sequence typing (MLST).

281 enes analyzed in multilocus sequence typing (MLST).

282 hisms (AFLP) and multilocus sequence typing (MLST).

283 y congruent with multilocus sequence typing (MLST).

284 ssigned by using multilocus sequence typing (MLST).

285 ere subjected to multilocus sequence typing (MLST).

286 were subtyped by multilocus sequence typing (MLST).

287 PCR (repPCR) and multilocus sequence typing (MLST).

288 d, and ribosomal multilocus sequence typing (MLST, eMLST, and rMLST); antigen gene sequence typing (A

289 Multi locus sequencing typing (MLST) analysis identified two strains of tsetse-associat

290 rigins (based on multilocus sequence typing [MLST] analysis), and did not arise recently.

291 s (determined by multilocus sequence typing [MLST]) and 79 pulsed-field gel electrophoresis types.

292 ned population of ~600,000 persons underwent MLST.

293 ST5, ST8, ST9, and ST30) were detected using MLST; the majority of MRSA isolates belonged to ST8, fol

294 Thus, while MLST was better for detecting genetic relatedness, we de

295 ily due to serotypes 1, 3, 19A, and 7F, with MLST demonstrating sequence types (ST) that were commonl

296 meningitidis strains are characterized with MLST as specific sequence types (ST) and clonal complexe

297 Compared with MLST, the assay has 100% sensitivity and 99.5% specifici

298 G45D mtrR mutations (p=0.046), but not with MLST or NG-MAST molecular types.

299 G45D mtrR mutations (P = .046) but not with MLST or NG-MAST types.

300 SERS typing correlated well with MLST indicating that it has high sensitivity and selecti