ライフサイエンスコーパス: MRM

1 MRM enabled reproducible, selective detection of the pep

2 MRM gave an apparent K(m) for GDP-L-fucose (GDP-Fuc) of

3 MRM has many similarities to mycoplasma respiratory dise

4 MRM has matured to the point that we can generate high c

5 MRM provided again the best results for CV efficiency (8

6 MRM transitions were established with capability to dist

7 MRM/MS revealed that unlike the rapid, modest (4-fold to

8 h the human genes and miRNAs, predicting 431 MRMs.

9 An MRM method was then established for verification.

10 bility of a large-scale effort to develop an MRM assay resource.

11 TMZ 150 to 200 mg/m(2) days 1 to 5 and MRM 50 mg days 8 to 28 was administered at 28-day interv

12 ped a high-throughput, SILAC-compatible, and MRM-based kinome profiling method and demonstrated that

13 ugh rapid profiling analyses, such as Q1 and MRM scans.

14 iency are obtained with the use of SIMCA and MRM (82.3 and 83.2% respectively), whereas MRM performs

15 The combination of TMZ and MRM resulted in a PFS at 6 months that exceeded the lite

16 The discriminating potential of the applied MRM approach was confirmed by differences among both 1D

17 lated peptides were specifically selected as MRM transitions.

18 s of brain regions; and correlations between MRM and classical histological data sets.

19 nd corpus callosum length can be detected by MRM before Abeta deposition.

20 3beta from whole cell lysate, we discover by MRM-MS a novel O-GlcNAcylated GSK-3beta peptide, bearing

21 ltiple signature peptides can be examined by MRM in a single experiment.

22 tial proteins were validated respectively by MRM and western blotting quantitative analyses.

23 cycle time from 2.4 s in conventional MRM (c-MRM) to 1 s in s-MRM allowed completion of the EPI scan

24 ic resolution by two endogenously contrasted MRM sequences.

25 total cycle time from 2.4 s in conventional MRM (c-MRM) to 1 s in s-MRM allowed completion of the EP

26 ures were used as landmarks when correlating MRMs with histological sections.

27 iplexing multiple reaction monitoring cubed (MRM(3)) assay for selective and sensitive quantification

28 After DI-MRM quantification was evaluated for standards, quantita

29 hods are compared; criteria for effective DI-MRM analysis are reported on the basis of the analysis o

30 Requirements for DI-MRM assay development are described.

31 lectrospray ionization, coupled with MRM (DI-MRM) is used for protein quantification.

32 The increased throughput of DI-MRM analysis is useful for rapid analysis of large batch

33 ins (HSPs) were translated from LC-MRM to DI-MRM for implementation in cell line models of multiple m

34 be comparable with standard isotope dilution MRM MS.

35 n analyte, the s-MRM algorithm monitors each MRM transition only around its expected retention time.

36 eaction monitoring mass spectrometry (LC/ESI-MRM-MS) are more commonly used in clinical research.

37 lly long analysis times per sample of LC/ESI-MRM-MS.

38 as well as 8-oxo-dGuo (as measured by LC-ESI/MRM/MS) and was enhanced by a catechol O-methyl transfer

39 eaction monitoring/mass spectrometry (LC-ESI/MRM/MS) assay.

40 y of a large-scale, international effort for MRM assay generation.

41 T were significantly less expensive than for MRM cases.

42 on monitoring with multistage fragmentation (MRM(3)) and differential mobility spectrometry (DMS) wer

43 All 11 participants who had MRM detected in posttreatment samples failed azithromyci

44 However, MRM could only detect four peptides (EVTEFAK, LVVITAGAR,

45 The combined integration of fast HPLC, MRM(3), and multiplexing yields an analysis workflow for

46 nsitivity than we have obtained by nano HPLC/MRM and substantially better than reported for LC/MS/MS.

47 lts demonstrate that high multiplexed immuno-MRM-MS assays are readily achievable using the optimized

48 eaction monitoring-mass spectrometry (immuno-MRM-MS) assay (n = 110) and applied it to measure candid

49 The improved MRM sensitivity described here delivered the highest 3D

50 -humulene were quantified using GC-QQQ-MS in MRM (multiple reaction mode) mode.

51 ximately 5-fold better than that observed in MRM or MIM without DMS.

52 ens of combined hormone contraceptive use in MRM and migraine with aura may decrease both headache fr

53 The positive ion MRM assay was more than sufficient to quantify endogenou

54 The precursor-to-product ion MRM transitions for alpha-endorphin, gamma-endorphin, an

55 Having established the parameters for LC-MRM MS, we quantified allergens from various commercial

56 hock proteins (HSPs) were translated from LC-MRM to DI-MRM for implementation in cell line models of

57 ss LC-QTOF MS for semi-polar metabolites, LC-MRM for oxylipins, and headspace GC-MS for volatile comp

58 le reaction monitoring mass spectrometry (LC-MRM MS) for rapid, accurate, and reproducible quantifica

59 le reaction monitoring mass spectrometry (LC-MRM) has emerged as a powerful platform for assessing pa

60 le reaction monitoring mass spectrometry (LC-MRM) was performed to identify differences in apoptosis

61 le reaction monitoring/mass spectrometry (LC-MRM/MS) technique that allows such determinations to be

62 Our data demonstrate that the LC-MRM MS method is valuable for absolute quantification of

63 The LC-MRM/MS method was rigorously validated using in vitro ki

64 ssion of approximately 300 kinases in two LC-MRM runs.

65 ubjected to relative quantification using LC-MRM-MS.

66 alytical performance to robust, validated LC-MRM methodology for RA quantification.

67 luated and compared to those obtained via LC-MRM and LC-MIM without DMS.

68 le reaction monitoring mass spectrometry (LC/MRM-MS) assays.

69 ed in a single multiplexed analysis using LC/MRM-MS.

70 ese proof-of-concept experiments using MALDI MRM-based imaging show the feasibility for the precise a

71 d strategy of microbial resource management (MRM).

72 trix metalloproteinase inhibitor marimastat (MRM) in patients with recurrent GBM was studied.

73 sis on costs of modified radical mastectomy (MRM) compared with breast-conserving surgery (BCS) and r

74 ergone either a modified radical mastectomy (MRM) or a segmental mastectomy with axillary dissection

75 velop a multiple reaction monitoring method (MRM) to detect the amounts of a particular polymorphism

76 The advent of MR microscopy (MRM) enables imaging biological samples at cellular reso

77 ltiparametric magnetic resonance microscopy (MRM) approach was applied to the Slovenian Kraski prsut

78 potential of magnetic resonance microscopy (MRM) for morphologic phenotyping in the mouse has previo

79 ly, the first magnetic resonance microscopy (MRM) images at the cellular level in isolated mammalian

80 Advances in magnetic resonance microscopy (MRM) make it practical to map gene variants responsible

81 Magnetic resonance microscopy (MRM) theoretically provides the spatial resolution and s

82 gh-resolution magnetic resonance microscopy (MRM) was used to determine regional brain volumetric cha

83 This approach, termed MIDAS (MRM-initiated detection and sequencing), is more sensiti

84 tions prevents menstrually related migraine (MRM) and migraine aura frequency.

85 e-quadrupole MS-based data acquisition mode (MRM).

86 ng in the multiple reaction monitoring mode (MRM) with collision-induced dissociation.

87 MS/MS) in multiple reaction monitoring mode (MRM).

88 onisation multiple reaction monitoring mode (MRM).

89 selected by multi-reaction monitoring mode (MRM).

90 he development of the mental rotation model (MRM) and the assertion that response preparation is medi

91 tions (PF), and multivariate range modeling (MRM)) were applied to multielement distribution to build

92 method to predict miRNA regulatory modules (MRMs) or groups of miRNAs and target genes that are beli

93 rometry in the multiple reaction monitoring (MRM) acquisition mode.

94 for scheduled multiple-reaction monitoring (MRM) analysis and adopted on-the-fly recalibration of re

95 ndard prior to multiple reaction monitoring (MRM) analysis enables prefractionation of the target pro

96 ding (SIR) and multiple reaction monitoring (MRM) and identified as methylcobalamin (Me-Cbl).

97 examined using Multiple Reaction Monitoring (MRM) as the acquisition mode.

98 d to develop a multiple reaction monitoring (MRM) assay that employed stable isotope-labeled peptide

99 ve developed a multiple reaction monitoring (MRM) assay to measure UCH-L1 in the high-speed supernata

100 ed hundreds of multiple reaction monitoring (MRM) assays for isotope ratio mass spectrometry of most

101 Multiple reaction monitoring (MRM) assays have proven successful for the absolute quan

102 tive method of multiple reaction monitoring (MRM) by mass spectrometry.

103 onstrated that multiple reaction monitoring (MRM) coupled with isotope dilution mass spectrometry can

104 efficiency for multiple reaction monitoring (MRM) detection.

105 designing QqQ multiple reaction monitoring (MRM) experiments for each of the 82 696 metabolites in t

106 combination of multiple reaction monitoring (MRM) fragment ratio normalization and chromatographic pe

107 ESI-MS/MS with multiple reaction monitoring (MRM) in the presence of deuterium-labeled internal stand

108 ry (PS-MS) and Multiple Reaction Monitoring (MRM) is described.

109 A rapid multiple reaction monitoring (MRM) mass spectrometric method for the detection and rel

110 uired by MALDI multiple reaction monitoring (MRM) mass spectrometry (MS), and accurate peptide quanti

111 ray ionization multiple reaction monitoring (MRM) mass spectrometry (MS).

112 Multiple reaction monitoring (MRM) mass spectrometry has been successfully applied to

113 ve developed a multiple reaction monitoring (MRM) mass spectrometry method to sensitively quantitate

114 In this study multiple reaction monitoring (MRM) mass spectrometry, viewed as the gold standard for

115 ate peptide by multiple-reaction monitoring (MRM) mass spectrometry.

116 and to build a multiple reaction monitoring (MRM) method with the MS/MS fragmentation pattern of the

117 rmed to create multiple reaction monitoring (MRM) methods for a wide range of PXDD/Fs from dihalogena

118 Multiple reaction monitoring (MRM) mode was used for LC-MS/MS.

119 S) method with multiple reaction monitoring (MRM) mode.

120 and DHT in the multiple-reaction monitoring (MRM) mode.

121 Multiple Reaction Monitoring (MRM) of the transition pairs of m/z 449.01 to 371.21 for

122 (LC-MS/MS) by multiple-reaction monitoring (MRM) on a triple quadrupole (QQQ) MS.

123 biosensor and multiple reaction monitoring (MRM) on a triple quadrupole mass spectrometer.

124 atography (LC) multiple reaction monitoring (MRM) quantification methods have necessitated lengthy ch

125 trometry using multiple reaction monitoring (MRM) showed more impressive data, with a 3-fold enrichme

126 We used a multiple reaction monitoring (MRM) to detect (13)C, D2-formaldehyde-modified OSCs by u

127 otocol employs multiple reaction monitoring (MRM) to search for all putative peptides specifically mo

128 own masses, or multiple reaction monitoring (MRM) transitions and are therefore often unable to detec

129 in 4 min using multiple reaction monitoring (MRM) transitions selective for each compound.

130 corresponding multiple reaction monitoring (MRM) transitions, and negative ions were approximately 1

131 Multiple reaction monitoring (MRM) was applied and the selection of proper product ion

132 ry method with multiple reaction monitoring (MRM) was employed to measure 264 lipid analytes extracte

133 e ion mode and multiple reaction monitoring (MRM) were used for LC-MS/MS.

134 Multiple reaction monitoring (MRM) with optimised transitions and collision energies f

135 quantified by multiple reaction monitoring (MRM), a mass spectrometry-based quantification method.

136 nsitivity, and multiple reaction monitoring (MRM), in the tandem mass spectrometric mode, was used to

137 the method of multiple reaction monitoring (MRM), we precisely and quantitatively measured the absol

138 ng predictable multiple reaction monitoring (MRM)-based quantitation with improved sensitivity.

139 established a multiple-reaction monitoring (MRM)-based targeted proteomic method that provided an un

140 hieved by UPLC/multiple-reaction monitoring (MRM)-MS, with analytical accuracies ranging from 87.4% t

141 mixture using multiple-reaction monitoring (MRM).

142 eveloped using multiple reaction monitoring (MRM).

143 the lysosome, multiple reaction monitoring (MRM)/mass spectrometry (MS) and polyubiquitin linkage-sp

144 d neutral-loss multiple-reaction-monitoring (MRM), and high-resolution mass spectrometry.

145 h to optimize multiple reactions monitoring (MRM) analysis and to confirm chromatographic retention t

146 activated carbon (AC); CETCO Organoclay MRM (MRM); Thiol-SAMMS (TS), a thiol-functionalized mesoporou

147 Glycosidase assisted LC-MS-MRM analysis of individual patient samples prepared by a

148 h multiple reaction monitoring (LC-ESI-MS/MS-MRM) to simultaneously measure levels of 5 mC and 5 hmC

149 ESI-MS/MRM is therefore a powerful tool for the kinetic charact

150 cally reactive allyl phosphate group, ESI-MS/MRM showed that there was no reduction in the concentrat

151 pled to multiple reaction monitoring (ESI-MS/MRM) has been applied for the first time to analyze enzy

152 nt fucosyltransferase (Fuc-T) V using ESI-MS/MRM.

153 mmary, we have established a multiplex LC/MS/MRM method for quantitatively profiling hundreds of know

154 acids), are sequentially analyzed on a LC/MS/MRM system.

155 detection and quantification of multiplexed, MRM-based assays, conducted by NCI-CPTAC.

156 The fast HPLC multiplexing MRM(3) assay demonstrated enhanced selectivity for endog

157 assessed for macrolide resistance mutations (MRMs) by high-resolution melt analysis.

158 disease in murine respiratory mycoplasmosis (MRM) and to select disease-resistant and nonresistant mo

159 Murine respiratory mycoplasmosis (MRM), due to Mycoplasma pulmonis infection, is an excell

160 mined by LC-Triple Quadrupole MS in negative MRM mode using external standard calibration.

161 ble as a resource to the community 645 novel MRM assays representing 319 proteins expressed in human

162 We report the ability of MRM-MS to detect a standard O-GlcNAcylated peptide and s

163 results of a systematic genetic analysis of MRM data using as a case study a family of well characte

164 nd computational methods for the analysis of MRM-MS data from proteins and peptides are still being d

165 Our results demonstrate the applicability of MRM for identification of HMPV, and assignment of geneti

166 On the basis of these assessments of MRM severity, one group of mouse strains was found to be

167 ur histologic lung lesions characteristic of MRM: alveolar exudate, airway exudate, airway epithelial

168 quantitation is determined from the ratio of MRM transitions for the endogenous unlabeled proteolytic

169 The use of ratios of MRM transition peak areas for corresponding peptides is

170 he remarkable sensitivity and selectivity of MRM enable the detection of low abundance IgG glycopepti

171 tyrosine in a PD model and the first use of MRM mass spectrometry to quantify changes in 3NT modific

172 While the use of MRM-MS assays is well established in the small molecule

173 : an activated carbon (AC); CETCO Organoclay MRM (MRM); Thiol-SAMMS (TS), a thiol-functionalized meso

174 Our MRM strategy, based on the application of successive, tr

175 A targeted multiplexed peptide MRM LC-MS/MS assay was used on a larger validation cohor

176 Positive MRMs trigger an MS/MS experiment to confirm the nature a

177 Pretreatment MRM was detected in 56 (36% [95% CI, 28%-43%]) participa

178 Here we review MRM capabilities and image segmentation methods; heritab

179 on (IDA) functionality was used to combine s-MRM with enhanced product ion (EPI) scans within the sam

180 .4 s in conventional MRM (c-MRM) to 1 s in s-MRM allowed completion of the EPI scan at the same time.

181 a scheduled multiple reaction monitoring (s-MRM) algorithm.

182 a known retention time of an analyte, the s-MRM algorithm monitors each MRM transition only around i

183 by selected/multiple reaction monitoring (S/MRM) or, on a larger scale, by SWATH (sequential window

184 ated with future work to develop large-scale MRM experiments.

185 Scheduled MRM improved the quality of the chromatograms, signal re

186 UPLC/scheduled MRM-MS with negative ion electrospray ionization enabled

187 a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers com

188 The interest of Scout-MRM method regarding the retention time independency, mu

189 xperience with analyzing a wide range of SID-MRM-MS data, we set forth a methodology for analysis tha

190 e reaction monitoring mass spectrometry (SIL/MRM-MS) has been frequently used to measure low-abundanc

191 immunoprecipitation in conjunction with SIL/MRM-MS assay which is capable of sensitive and accurate

192 tiple reaction monitoring mass spectrometry (MRM MS) with (15)N-labeled full-length apoE4 as an inter

193 tiple reaction monitoring mass spectrometry (MRM-MS) analysis of the nonglycopeptides, the assay can

194 tiple reaction monitoring mass spectrometry (MRM-MS) with stable isotope dilution (SID) is increasing

195 tiple Reaction Monitoring Mass Spectrometry (MRM-MS), a targeted MS method, to detect and quantify na

196 tiple reaction monitoring mass spectrometry (MRM-MS), and the resultant candidate biomarkers were the

197 emerged as black, spherical elements on T2* MRMs and could be distinguished from vessels only in cro

198 d in all fortified samples, a challenge that MRM-transition could not address in a single step.

199 We found that MRM can be used to image single myofibers with 6-mum res

200 the MRM and furosine results indicated that MRM based on tryptic digests of whole products was a fea

201 Recent studies continue to support that MRM is precipitated by drops in estrogen concentrations,

202 The MRM method was developed from a knowledge of peptide fra

203 The MRM results reported provide a new direction for applyin

204 The MRM results showed an increase in peak areas of the two

205 The MRM UHPLC-MS/MS method, Western blot and RT-PCR were use

206 A good correlation between the MRM and furosine results indicated that MRM based on try

207 To determine if the MRM can be extrapolated to perceptually familiar angles

208 Consistent with the MRM, experiment one revealed a linear increase in RT as

209 Rasa Aragonesa sheep was analyzed using this MRM method.

210 The combination of high throughput MRM and genomics will improve our understanding of the g

211 SWATH-MS was comparable (p < 0.001) to MRM-MS for 32/33 peptides assessed across the four famil

212 on of the data showed that ESI-MS coupled to MRM is a valid approach for the analysis of enzyme kinet

213 We concluded that resistance to MRM is a complex trait.

214 At least two MRM transitions were used to quantify and identify each

215 onventional approach with LC-MS/MS using two MRM transitions produced the same identifications and co

216 mes the sensitivity challenge in the typical MRM method due to poor CID fragmentation of the analyte.

217 live oil have been assayed by LC-MS/MS under MRM condition and isotope dilution method, using d(2)-la

218 compensate for over recovery observed under MRM-transition mode.

219 The assay has been performed under MRM condition monitoring two transitions for each analyt

220 stically different than for women undergoing MRM.

221 ere eligible for our study; 44 had undergone MRM, and 50 had undergone SegAx/XRT.

222 nt ion transitions were used to perform UPLC-MRM-MS for untargeted detection of the structural isomer

223 -reaction monitoring-mass spectrometry (UPLC-MRM-MS) method for the separation and detection of 50 kn

224 In summary, this UPLC-MRM-MS method has enabled the quantitation of the larges

225 We used MRM to obtain 3D volumetric data on mouse brains imaged

226 The limits of detection (S/N =3) using MRM are 20 pg for Ang IV and 25 pg for Ang 1-7, Ang III,

227 We have developed a method using MRM to monitor protein glycosylation normalized to absol

228 ation of target peptides was performed using MRM on a LC/triple-quad MS/MS using (12)C- (control) and

229 ivity and accuracy of the quantitation using MRM were determined, with the detection limit in the fem

230 nowledge, this is the first report utilizing MRM-MS to detect native O-GlcNAc modified peptides.

231 d MRM (82.3 and 83.2% respectively), whereas MRM performs better than SIMCA in terms of forced model

232 decreased background signal associated with MRM.

233 o generate reproducible data comparable with MRM-MS, but has the added benefits of allowing reinterro

234 ed nanoelectrospray ionization, coupled with MRM (DI-MRM) is used for protein quantification.

235 pic and quantotypic peptides to proceed with MRM method development.

236 ly $450 per month higher than for women with MRM.