1 aling involve inhibition of the Cdc20-APC by Mad2 protein.

2                              The metamorphic Mad2 protein acts as a molecular switch in the checkpoin

3 veal an important role for Tpr in which Mad1-Mad2 proteins are regulated during the cell cycle and mi

4  premature anaphase onset [9,10], and excess Mad2 protein causes arrest in mitosis [5,11].

5  APC inhibition by Emi1 and find that unlike Mad2 proteins, Emi1 binds and inhibits both APC(Cdh1) an

6                   These studies suggest that Mad2 protein function is essential for the timing of ana

7 xel resistance and whether overexpression of Mad2 protein in checkpoint-defective cells enhances pacl

8 SF arrest by Mos is mediated by the Mad1 and Mad2 proteins in a manner distinct from the spindle chec

9 Here, we show that microinjection of Mad1 or Mad2 protein into early Xenopus laevis embryos causes me

10                                In yeast, the Mad2 protein is required for the M phase arrest induced

11 odepletion analysis shows that the Mad1-free Mad2 protein is unable to bind Cdc20, consistent with th

12 tic cells, we found that severe reduction of MAD2 protein levels results in mitotic failure and exten

13  induced Mad2 phosphorylation and suppressed Mad2 protein levels.

14 king Mad2(S195D) mutant and the phospho-S195 Mad2 protein obtained using intein-mediated semisynthesi

15                        We tested whether the Mad2 protein participates in regulating the timing of an

16 ub) 1, mitotic arrest-deficient (Mad) 1, and Mad2 proteins promote this checkpoint through sustained

17                                    The yeast MAD2 protein was first identified in a genetic screen to

18                                              Mad2 protein was increased as a consequence of the absen

19                              The increase in Mad2 protein was observed also at the cellular level by

20 y player in the checkpoint's response is the Mad2 protein, which prevents activation of the anaphase-

21 appears to be the human homolog of the yeast MAD2 protein, which we term hMAD2.