ライフサイエンスコーパス: Maskin

1 escued by readdition of purified full-length maskin.

2 e points, cannot be rescued by readdition of maskin.

3 scued by addition of only the TACC-domain of maskin.

4 lyadenylation, are colocalized with CPEB and maskin.

5 ylation, interacts with a new factor we term maskin.

6 translational silencing of cyclin B1 mRNA by Maskin, a CPEB and eIF4E binding factor, whose expressio

7 s polyadenylation hexanucleotide AAUAAA; and maskin, a CPEB and eIF4E binding protein whose regulatio

8 Such particles also contain maskin, a CPEB-associated factor that mediates cap-depen

9 nylation take place, the interaction between maskin, a CPEB-associated factor, and eIF4E, the cap-bin

10 CPEB also binds maskin, a protein that in turn interacts with eIF4E, the

11 These results show that Maskin activity and localization is controlled by differ

12 emble those present in eIF4G, 4EBP, Cup, and Maskin, all of which are eIF4E binding proteins.

13 In oocytes, Maskin also binds eukaryotic translation initiation fact

14 te that CPEB and maskin bind directly, as do maskin and elF-4E.

15 nown to promote microtubule assembly such as Maskin and XMAP215.

16 inity chromatography demonstrates that CPEB, maskin, and elF-4E reside in a complex in oocytes, and y

17 slation in Xenopus oocytes (CPEB, CPSF, PAP, maskin, and IAK1, the murine homologue of Eg2) are also

18 us oocytes involve the eIF4E-binding protein maskin as the key factor for the repression of maternal

19 ck experiments demonstrate that maskin, or a maskin-associated activity, is required for two distinct

20 t two-hybrid analyses indicate that CPEB and maskin bind directly, as do maskin and elF-4E.

21 a inhibits the regulatory phosphorylation of maskin by Aurora-A.

22 Maskin contains a peptide sequence that is conserved amo

23 Surprisingly, CPEB, Maskin, CPSF, and several other factors involved in poly

24 sufficient to restore centrosome function in maskin-depleted extracts, and we provide evidence that t

25 duced aster size during early time points in maskin-depleted extracts, can be rescued by readdition o

26 Maskin depletion from egg extracts results in compromise

27 cs in Xenopus egg extracts are unaffected by maskin depletion.

28 Dissociation of the maskin-eIF4E complex is essential for cyclin B1 mRNA tra

29 emain together during oocyte maturation, the maskin-elF-4E interaction is substantially reduced.

30 ch in turn binds eIF4G and helps it displace Maskin from eIF4E, thereby inducing translation.

31 alleviates the cdk1-induced dissociation of Maskin from eIF4E.

32 called into doubt by recent findings, is the maskin hypothesis for translational repression of cyclin

33 Maskin immunodepletion and add-back experiments demonstr

34 e direct experimental evidence of a role for maskin in centrosome function and suggest that maskin is

35 blastomeres has been attributed to a role of maskin in regulating the translation of, among others, c

36 d we provide evidence that the N terminus of maskin inhibits the function of the TACC domain.

37 We show that maskin interacts with a number of proteins in egg extrac

38 l mRNA, a second mechanism must exist, since maskin is absent earlier in oogenesis.

39 reported role as a translational regulator, maskin is also important for mitotic spindle assembly.

40 The conserved C-terminal TACC domain of maskin is both necessary and sufficient to restore centr

41 Prior to maturation, Maskin is phosphorylated on S626 by protein kinase A.

42 ssays, we show that the Xenopus TACC protein maskin is required for centrosome function beyond recrui

43 skin in centrosome function and suggest that maskin is required for microtubule anchoring at the cent

44 Maskin is the Xenopus homolog of the transforming acidic

45 Here, we show that although maskin lacks a canonical nuclear localization sequence,

46 In this study, we show that maskin, like other TACC proteins, plays a direct role in

47 on and add-back experiments demonstrate that maskin, or a maskin-associated activity, is required for

48 teins expressed late in oogenesis, including maskin, PARN, and 4E-BP1.

49 yadenylation element-binding protein (CPEB), maskin, poly(A) polymerase, cleavage and polyadenylation

50 antibody and mRNA reporter injection assays, maskin prevents oocyte maturation and the translation of

51 manner similar to the action of the Xenopus Maskin protein.

52 While CPEB and maskin remain together during oocyte maturation, the mas

53 We identified maskin, the Xenopus member of the transforming acidic co

54 CPEB and maskin, two factors that control polyadenylation-induced

55 Here we show that Maskin undergoes several phosphorylation events during o

56 e CPEB, which binds directly to the CPE, and Maskin, which associates with CPEB.

57 ation in Xenopus oocytes by a protein called Maskin, which purportedly interacts with initiation fact