ライフサイエンスコーパス: Methanosarcina

1 le for genetic studies in the archaeal genus Methanosarcina.

2 stic methanogenesis in both Methanosaeta and Methanosarcina.

3 ed a markerless tRNA(Pyl) deletion strain of Methanosarcina acetivorans (DeltapylT) that cannot decod

4 t comparison of histones from M. jannaschii, Methanosarcina acetivorans (largest Archaeal genome, 5.8

5 ere identified in M. jannaschii (Mj0601) and Methanosarcina acetivorans (Ma2851), and recombinant Ma2

6 TLPs from Bacillus thuringiensis (BtTLP) and Methanosarcina acetivorans (MaTLP) display biochemical p

7 naturally "truncated" homologs of NifB from Methanosarcina acetivorans (NifB(Ma)) and Methanobacteri

8 old/one zinc finger replication protein A in Methanosarcina acetivorans and Methanopyrus kandleri exh

9 ence of Methanosarcina barkeri with those of Methanosarcina acetivorans and Methanosarcina mazei.

10 e isozymes of methanol methyltransferases in Methanosarcina acetivorans C2A and are among the most hi

11 The mtaA1, mtaA2, and mtbA genes of Methanosarcina acetivorans C2A encode putative methanol-

12 Methanosarcina acetivorans C2A encodes three putative hy

13 Methanosarcina acetivorans C2A is able to convert severa

14 clones were isolated after transformation of Methanosarcina acetivorans C2A with the mutagenized plas

15 en in this process, we tested the ability of Methanosarcina acetivorans C2A, a metabolically versatil

16 oson mutagenesis of a methanogenic archaeon, Methanosarcina acetivorans C2A, which because of its ind

17 The corresponding mutation in Methanosarcina acetivorans C2A, which cannot grow on H(2

18 sequence of an acetate-utilizing methanogen, Methanosarcina acetivorans C2A.

19 ere the first use of directed mutagenesis in Methanosarcina acetivorans C2A.

20 s and characterized the encoded enzymes from Methanosarcina acetivorans C2A.

21 lyses to study the structure/function of the Methanosarcina acetivorans clamp loader or replication f

22 rolysine, a 761 base-pair genomic segment in Methanosarcina acetivorans containing the pylT gene (enc

23 The genome of Methanosarcina acetivorans encodes three homologs, initi

24 Interestingly, the euryarchaeon Methanosarcina acetivorans harbors multiple functional R

25 Unlike most organisms, Methanosarcina acetivorans harbors multiple functional R

26 The Methanosarcina acetivorans hdrED1 operon is constitutive

27 on of the slow-growing methanogenic archaeon Methanosarcina acetivorans Introduction of both insertio

28 ation of McrA from the methanogenic archaeon Methanosarcina acetivorans lacking tfuA and/or ycaO reve

29 se 1 operons (mtaCB1, mtaCB2, and mtaCB3) in Methanosarcina acetivorans led to the suggestion that ea

30 n methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically labeled with 14

31 The regulation of the Methanosarcina acetivorans mtsD, mtsF and mtsH genes, wh

32 standing of this family, a flavoredoxin from Methanosarcina acetivorans of the Archaea domain was pro

33 Here we show that Methanosarcina acetivorans possesses three operons encod

34 Methanosarcina acetivorans produces acetate, formate, an

35 , 3046 unique peptides covering 566 distinct Methanosarcina acetivorans proteins were identified from

36 tandem polypeptide repeats that comprise the Methanosarcina acetivorans S-layer protein and propose a

37 haea and that the polymerase from mesophilic Methanosarcina acetivorans shows identical behaviour.

38 Methanosarcina acetivorans strain C2A is a marine methan

39 Methanosarcina acetivorans strains bearing deletions of

40 ein the purification and characterization of Methanosarcina acetivorans subunit D in complex with sub

41 MacDinB-1, the homolog from the euryarchaeon Methanosarcina acetivorans that is characterized in this

42 The Methanosarcina acetivorans Thg1 (MaThg1) gene contains a

43 otein overproduction system was developed in Methanosarcina acetivorans to facilitate biochemical cha

44 Here, we study the methanogenic archaeon Methanosarcina acetivorans using assays of ATP hydrolysi

45 ltisubunit sodium/proton antiporter (Mrp) of Methanosarcina acetivorans was investigated with a mutan

46 rotein MA4561 from the methanogenic archaeon Methanosarcina acetivorans was originally predicted to b

47 ologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using ge

48 Methanosarcina acetivorans, a strictly anaerobic methane

49 nococcus jannaschii, Archaeoglobus fulgidus, Methanosarcina acetivorans, and Methanosarcina barkeri p

50 chaeoglobus fulgidus, Methanopyrus kandleri, Methanosarcina acetivorans, and Methanosarcina mazei.

51 bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene dele

52 Gene-deletion and growth experiments in Methanosarcina acetivorans, using sulfide as the sole su

53 a representative of this new group of RPA in Methanosarcina acetivorans, we made two deletion mutants

54 termed Hsp60-4 and Hsp60-5, in the archaeon Methanosarcina acetivorans, which also has Hsp60-1, Hsp6

55 a new form of clamp loader from the archaeon Methanosarcina acetivorans.

56 MaPgb from the strictly anaerobic methanogen Methanosarcina acetivorans.

57 ogs, RPA1, RPA2, and RPA3, from the archaeon Methanosarcina acetivorans.

58 on the naturally occurring plasmid pC2A from Methanosarcina acetivorans.

59 rized a member of the YrbG family, MaX1 from Methanosarcina acetivorans.

60 mp loader (RFC) from the mesophilic archaeon Methanosarcina acetivorans.

61 e family from the methane-producing archaeon Methanosarcina acetivorans.

62 d in Escherichia coli expressing pylBCD from Methanosarcina acetivorans.

63 methyltransferase MtmB1, was introduced into Methanosarcina acetivorans.

64 Although the aceticlastic methanoarchaea Methanosarcina and Methanosaeta employ different enzymes

65 me, genetic analysis in Archaea of the genus Methanosarcina are presented.

66 RNA pyrosequencing revealed the dominance of Methanosarcina at 55-60 degrees C.

67 Methanosarcina barkeri 227 possesses two clusters of gen

68 nitrogenase structural genes (nifHDK2) from Methanosarcina barkeri 227 was completed in this study b

69 tial reactions of acetyl-CoA cleavage by the Methanosarcina barkeri acetyl-CoA decarbonylase synthase

70 io desulfuricans, Desulfovibrio vulgaris and Methanosarcina barkeri AhbA/B have been produced and the

71 meric iron-sulfur protein, was isolated from Methanosarcina barkeri and is required for in vitro ATP-

72 s of these putative hydrogenases to those of Methanosarcina barkeri and Methanosarcina mazei shows th

73 Species close to Methanosarcina barkeri and Methanothermobacter thermauto

74 extend earlier findings that the CODHs from Methanosarcina barkeri and Oligotropha carboxydovorans e

75 We show here that wild type PylS from Methanosarcina barkeri and PylSn from Desulfitobacterium

76 iting domain homologues (AlaX proteins) from Methanosarcina barkeri and Sulfolobus solfataricus hydro

77 most similar to those of the archaebacterium Methanosarcina barkeri and the delta-purple bacterium De

78 volution reaction (HER) electrocatalysts and Methanosarcina barkeri as a biocatalyst for CO2 fixation

79 ine methyltransferase of the archaebacterium Methanosarcina barkeri contains a novel amino acid, pyrr

80 ethylamine methyltransferase of the archaeon Methanosarcina barkeri contains a rare amino acid, pyrro

81 coli strain NK3 led to the isolation of the Methanosarcina barkeri cysK gene [encoding O-acetylserin

82 However, Methanosarcina barkeri Delta fpo mutants constructed in

83 lamine methyltransferase, were detectable in Methanosarcina barkeri during early log growth on trimet

84 Activity staining of extracts of Methanosarcina barkeri electrophoresed in polyacrylamide

85 During growth on acetate, Methanosarcina barkeri expresses catabolic enzymes for o

86 In the methanogenic archaeon Methanosarcina barkeri Fusaro, the N5-methyl-tetrahydros

87 e isoleucyl-tRNA synthetase gene (ileS) from Methanosarcina barkeri Fusaro.

88 Methanosarcina barkeri genes cmtA and cmtM encoding both

89 enzyme MT2-A important for methanogenesis in Methanosarcina barkeri growing on methylamines.

90 plays a central role in energy metabolism in Methanosarcina barkeri grown on acetate.

91 llireducens with Geobacter sulfurreducens or Methanosarcina barkeri in which ethanol was the electron

92 These proteins isolated from Methanosarcina barkeri included the previously unidentif

93 onally inserted amino acid, was found in the Methanosarcina barkeri monomethylamine methyltransferase

94 a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase

95 We demonstrate that an orthogonal Methanosarcina barkeri MS pyrrolysyl-tRNA synthetase/tRN

96 e proteins are clustered on 6.8 kb of DNA in Methanosarcina barkeri MS.

97 A hexahistidine-tagged Methanosarcina barkeri mtmB1 gene, encoding monomethylam

98 A series of Methanosarcina barkeri mutants lacking the genes encodin

99 that transcripts of nifHDK2 genes, encoding Methanosarcina barkeri nitrogenase, are present in N2-gr

100 Evidence is presented that, in Methanosarcina barkeri oxaloacetate synthesis, an essent

101 cryptofilum PE (CkoPE; 117 amino acids) and Methanosarcina barkeri PE (MbaPE; 151 amino acids)--and

102 us fulgidus, Methanosarcina acetivorans, and Methanosarcina barkeri possess open reading frames with

103 hetase-tRNA(CUA) (MjTyrRS-tRNA(CUA)) and the Methanosarcina barkeri pyrrolysyl-tRNA synthetase-tRNA(C

104 The Methanosarcina barkeri serC gene, encoding phosphoserine

105 lobus acidocaldarius, Haloferax volcanii and Methanosarcina barkeri the secondary structure of RNase

106 Forty-two Methanosarcina barkeri tRNA(Pyl) variants were tested in

107 sozymes from buffer soluble cell extracts of Methanosarcina barkeri was accomplished by use of immobi

108 recently discovered coenzyme M methylase in Methanosarcina barkeri were analyzed.

109 mparative analysis of the genome sequence of Methanosarcina barkeri with those of Methanosarcina acet

110 mbrane-bound NiFe-hydrogenase complexes from Methanosarcina barkeri, Escherichia coli, and Rhodospiri

111 Like the HDR from Methanosarcina barkeri, it was found to contain two disc

112 Analyses of Methanosarcina thermophila, Methanosarcina barkeri, Methanobacterium thermoautotroph

113 fer RNA (tRNA) synthetases (SerRSs) exist in Methanosarcina barkeri, one of bacterial type and the ot

114 the metabolic networks of Escherichia coli, Methanosarcina barkeri, Staphylococcus aureus, and Sacch

115 ibody detected primarily a 50-kDa protein in Methanosarcina barkeri, which is the mass predicted for

116 the chromosome of the methanogenic archaeon Methanosarcina barkeri.

117 vinelandii, Rhodopseudomonas palustris, and Methanosarcina barkeri.

118 on in methylamine methyltransferase genes of Methanosarcina barkeri.

119 These in vitro data suggest that Methanosarcina cells have two pathways for acylating the

120 We propose that MA3736 be renamed mdrA (methanosarcina disulfide reductase).

121 w that the efficient acetoclastic pathway in Methanosarcina emerged at a time statistically indisting

122 The ileS gene was cloned into a Methanosarcina-Escherichia coli shuttle vector and mutag

123 ented in trans with autonomously replicating Methanosarcina-Escherichia plasmid shuttle vectors.

124 horizontally transferred to the ancestor of Methanosarcina from a derived cellulolytic organism in t

125 l lysine codon usage is encountered in other Methanosarcina genes.

126 ) and ADP-Acs, the extant methanogenic genus Methanosarcina is the only identified Archaeal genus tha

127 elated class II photolyase from the archaeon Methanosarcina mazei (MmCPDII) as well as plantal orthol

128 Characterization of SepRS from the mesophile Methanosarcina mazei by gel filtration and nondenaturing

129 In contrast, Methanosarcina mazei contains seven AAA proteins, five o

130 ly expressed genes of the freshwater isolate Methanosarcina mazei determined by transcriptional profi

131 the A-type molecular motor A3B3DF, from the Methanosarcina mazei Go1 A-ATP synthase, and the thermop

132 e determined three crystal structures of the Methanosarcina mazei PylRS complexed with either AMP-PNP

133 e demonstrate that stable integration of the Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)/

134 3', were found in the genome of the archaeon Methanosarcina mazei S-6 that encode the deduced protein

135 nases to those of Methanosarcina barkeri and Methanosarcina mazei shows that each predicted subunit c

136 the ATP:co(I)rrinoid adenosyltransferase in Methanosarcina mazei strain Go1 (open reading frame MM31

137 me (ORF) Mm2058 of the methanogenic archaeon Methanosarcina mazei strain Go1 was shown in vivo and in

138 The cbiZ gene of the methanogenic archaeon Methanosarcina mazei strain Gol was cloned, was overprod

139 Here we show that the archaebacterial Methanosarcina mazei ThrRS efficiently misactivates seri

140 s that exhibit topology simplification), and Methanosarcina mazei topo VI and Sulfolobus shibatae top

141 dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mug (6 m

142 propose that MM1854, a homolog of AfpA from Methanosarcina mazei, catalyzes the last step of H4MPT b

143 alophilic Archaea (Methanococcus jannaschii, Methanosarcina mazei, Methanobrevibacter smithii) are le

144 us kandleri, Methanosarcina acetivorans, and Methanosarcina mazei.

145 25 of Deinococcus radiodurans and MM_0920 of Methanosarcina mazei.

146 s, with Fo and P(i) or with Fo and GDP, from Methanosarcina mazei.

147 l three enzymes in the mesophilic methanogen Methanosarcina mazei.

148 MK enzymes from alternative microbes such as Methanosarcina mazei.

149 with those of Methanosarcina acetivorans and Methanosarcina mazei.

150 ragment of chromosomal DNA from the archaeon Methanosarcina mazeii was sequenced and analyzed, and it

151 cus of the mesophilic, methanogenic archaeon Methanosarcina mazeii.

152 , Pyrobaculum, Aeropyrum) and euryarchaeota (Methanosarcina, Methanococcus, Archaeoglobus, Thermoplas

153 cts translation termination, its presence in Methanosarcina mRNA may lead to pyrrolysine (Pyl) incorp

154 at express the Himar1 transposase from known Methanosarcina promoters.

155 Enzymes from species within Methanosarcina, Pseudomonas, Bartonella, Nitrosomonas, T

156 a strong increase of the expression level in Methanosarcina sp. was evidenced after oleate addition.

157 with multicellular life-cycle phases, e.g., Methanosarcina sp., or Anabaena sp., which have more per

158 ry selectable markers that are functional in Methanosarcina species and that express the Himar1 trans

159 de resistance to pseudomonic acid (PA(r)) in Methanosarcina species by mutagenesis of the isoleucyl-t

160 Methanosarcina species possess three operons (mtaCB1, mt

161 dimethylamine, or monomethylamine by various Methanosarcina species possesses one naturally occurring

162 vidence suggests that methanol catabolism in Methanosarcina species requires the concerted effort of

163 acetivorans were aligned with those in other Methanosarcina species to identify conserved transcripti

164 ency in methylamine metabolism expected of a Methanosarcina species unable to decode UAG codons as py

165 olving Ech hydrogenase complex of freshwater Methanosarcina species with the Rnf complex, which gener

166 region is well conserved among the sequenced Methanosarcina species, while the second vht-type homolo

167 tbp1 is strictly conserved in the genomes of Methanosarcina species.

168 Methanosarcina spp. begin methanogenesis from methylamin

169 is well conserved with respect to the other Methanosarcina spp. in the region proximal to the origin

170 dehydrogenase/acetyl coenzyme A synthase in Methanosarcina spp. is coordinately regulated in respons

171 sual possession of both LysRS1 and LysRS2 by Methanosarcina spp. may also reflect differences in cata

172 These vectors replicate in 9 of 11 Methanosarcina strains tested and in Escherichia coli.

173 tate kinases from E. coli (Bacteria domain), Methanosarcina thermophila (Archaea domain), and four ot

174 ototypic gamma-class carbonic anhydrase from Methanosarcina thermophila (Cam) were characterized by s

175 and E84A carbonic anhydrase from the archeon Methanosarcina thermophila (gamma class).

176 etermined the three-dimensional structure of Methanosarcina thermophila acetate kinase bound to ADP t

177 denosine pocket as compared to ATP-utilizing Methanosarcina thermophila ACK (MtACK).

178 , has been established in the methanoarchaea Methanosarcina thermophila and Methanococcus jannaschii.

179 two different members of the domain Archaea, Methanosarcina thermophila and Sulfolobus solfataricus.

180 e of nickel in this process two forms of the Methanosarcina thermophila beta subunit were overexpress

181 scribe the inhibition of acetate kinase from Methanosarcina thermophila by preincubation with MgCl(2)

182 cetate kinase is the homodimeric enzyme from Methanosarcina thermophila containing ADP and sulfate in

183 The CO dehydrogenase enzyme complex from Methanosarcina thermophila contains a corrinoid/iron-sul

184 HDR from Methanosarcina thermophila contains two b-hemes and two

185 Inspection of the crystal structure of the Methanosarcina thermophila enzyme containing only ADP re

186 recently determined crystal structure of the Methanosarcina thermophila enzyme identifies it as a mem

187 The enzyme from Methanosarcina thermophila has been cloned and hyper-pro

188 Isf (iron-sulfur flavoprotein) from Methanosarcina thermophila has been produced in Escheric

189 A catalytic mechanism for the enzyme from Methanosarcina thermophila has been proposed on the basi

190 ransacetylase from the methanogenic archaeon Methanosarcina thermophila in complex with the substrate

191 c analysis of the phosphotransacetylase from Methanosarcina thermophila indicated that there is a ter

192 rogenase/acetyl-CoA synthase (CODH/ACS) from Methanosarcina thermophila is part of a five-subunit com

193 k, the reaction catalyzed by the enzyme from Methanosarcina thermophila is shown to be analogous to o

194 The homotrimeric enzyme Mt-Cam from Methanosarcina thermophila is the archetype of the gamma

195 taining carbonic anhydrase from the archaeon Methanosarcina thermophila suggests that a very similar

196 nic anhydrase from the thermophilic archaeon Methanosarcina thermophila that exhibits no significant

197 zyme complex was isolated from acetate-grown Methanosarcina thermophila that oxidized CO and catalyze

198 ataricus ATCC 35091, Haloferax volcanii, and Methanosarcina thermophila TM-1, representing the Euryar

199 and sequenced from the methanogenic archaeon Methanosarcina thermophila TM-1.

200 way for the biosynthesis of methanopterin in Methanosarcina thermophila to proceed by the following s

201 oding an iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila was cloned and sequenced.

202 ed against the gamma carbonic anhydrase from Methanosarcina thermophila was detected.

203 The gene encoding carbonic anhydrase from Methanosarcina thermophila was hyperexpressed in Escheri

204 e catalytic mechanism of acetate kinase from Methanosarcina thermophila was investigated by diethylpy

205 The 20S proteasome from the methanoarchaeon Methanosarcina thermophila was produced in Escherichia c

206 The heterodisulfide reductase (HDR) from Methanosarcina thermophila was purified to homogeneity f

207 the prototypic gamma-carbonic anhydrase from Methanosarcina thermophila were characterized by extende

208 yl-coenzyme synthase (CODH/ACS) complex from Methanosarcina thermophila were cloned and sequenced.

209 s of the iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila were identified in databases.

210 site A-cluster in the ACDS beta subunit from Methanosarcina thermophila were investigated.

211 bonic anhydrase Cam (carbonic anhydrase from Methanosarcina thermophila) was investigated.

212 systems from Clostridium thermoaceticum and Methanosarcina thermophila).

213 characterization of OASS from acetate-grown Methanosarcina thermophila, a moderately thermophilic me

214 t A cluster proteins, ACDS beta subunit from Methanosarcina thermophila, acetyl-CoA synthase of Carbo

215 ichia coli of the phosphotransacetylase from Methanosarcina thermophila, an obligately anaerobic meth

216 purified from the methane-producing archaeon Methanosarcina thermophila, and the N-terminal sequence

217 Cam, from the procaryote Methanosarcina thermophila, is the prototype of the gamm

218 Analyses of Methanosarcina thermophila, Methanosarcina barkeri, Meth

219 flavoprotein (Isf) from the methanoarchaeaon Methanosarcina thermophila, which participates in electr

220 also found in the methanogenic CODH/ACS from Methanosarcina thermophila.

221 by using cell extracts of M. jannaschii and Methanosarcina thermophila.

222 estigated for the phosphotransacetylase from Methanosarcina thermophila.

223 characterized from the methanogenic archaeon Methanosarcina thermophila.

224 osphate) and biotin, has been established in Methanosarcina thermophila.

225 ive form of methyl-SCoM reductase (MCR) from Methanosarcina thermophila.

226 se II, and the gamma-carbonic anhydrase from Methanosarcina thermophilain an effort to outline common

227 Because there are no known Methanosarcina transposons we modified the mariner trans

228 increase to 65 degrees C resulted in loss of Methanosarcina, with an accumulation of organic acids an