ライフサイエンスコーパス: MgCl2

1 MgCl2 days showed lower PM10 mean concentrations, but th

2 MgCl2 increased the activity of the NADP-dependent enzym

3 MgCl2-CaCl2 composite salts were selected, and their flu

4 (with 1 mM MgCl2 in cis intracellular and 0 MgCl2 in trans extracellular solutions, pH in both solut

5 ion containing DNA or denatured protein on a MgCl2-soaked mica surface.

6 In addition, MgCl2, MnCl2, and CaCl2 allowed some peptidase and casei

7 Pase activity in the presence of excess ADP, MgCl2, and NaF.

8 n electron microscopy in the presence of ADP/MgCl2 Single-particle analysis yielded a low-resolution

9 bohedral layered CdCl2-type structure (alpha-MgCl2) has been studied experimentally using synchrotron

10 zyme with MgCl2 followed by equimolar AMPCPP/MgCl2.

11 When reaction mixtures containing ATP and MgCl2 were boiled to terminate the enzyme assay, a cAMP-

12 The CaCl2 and MgCl2 addition increased the gel strength during rheolog

13 influence of pH (3.5 and 7.0) and CaCl2 and MgCl2 addition on heat-set gelation of a quinoa protein

14 of GO concentration and in NaCl, CaCl2, and MgCl2 as a function of ionic strength (IS).

15 s (CCC) were determined for NaCl, CaCl2, and MgCl2 electrolytes.

16 n if the vaporizer is frequently cleaned and MgCl2 concentration in water is relatively low.

17 eness of calcium-magnesium acetate (CMA) and MgCl2 in reducing road dust emissions in a Mediterranean

18 re substrate instead of the ice crystals and MgCl2 hydrates.

19 By adjusting the amounts of blocking DNA and MgCl2, we prepared slides that contained many straight p

20 ing the phosphatase activators glutamate and MgCl2 or treatment of immunoprecipitated ACC-beta with p

21 ivity and is dependent on NTP hydrolysis and MgCl2.

22 function of physiologically relevant KCl and MgCl2 concentrations, and we found that this activity wa

23 optima differ with respect to pH and KCl and MgCl2 concentrations.

24 larity to quinine was found only for KCl and MgCl2.

25 e, is sensitive to concentrations of KCl and MgCl2; addition of 3 mM MgCl2 to 100 mM KCl changes the

26 RPA-DNA complexes was assessed, and NaCl and MgCl2 concentrations that completely inhibited rhRPA bin

27 For NaCl and MgCl2 solution, the NaCl hydrates prefer to crystallize

28 hanges in the distributions of both NaCl and MgCl2, DeltaG(Mg)org and DeltaDeltaG(Na)org.

29 of the concentration of NaCl, KCl, NaF, and MgCl2 at pH 7.5, 21 degrees C.

30 of T7 RNA polymerase, DNA template, NTP and MgCl2 proved to be significantly correlated with the yie

31 Contrary to expectation, CdCl2-processed and MgCl2-processed solar cells contain similar concentratio

32 ibit similar pH optima, heat stabilities and MgCl2 requirements, but differ in their requirements for

33 cids is enhanced by the addition of ThDP and MgCl2.

34 Divalent cations such as MgCl2 and CaCl2 stimulate the binding of 125I-glucagon t

35 e and mild basic reaction conditions such as MgCl2 and dicyclohexylmethylamine for the deprotonation

36 compared to high-grade Mg-compounds such as MgCl2.

37 s-linking was done after incubation in ATP + MgCl2.

38 At 1 hr after CLP, ATP-MgCl2 (50 micromol/kg body weight) or an equivalent volu

39 Administration of ATP-MgCl2 after the onset of sepsis, however, maintained ACh

40 ine whether or not the administration of ATP-MgCl2 early after the onset of sepsis improves or mainta

41 Since administration of ATP-MgCl2 prevents the impaired vascular relaxation to the e

42 erved, irrespective of administration of ATP-MgCl2.

43 Although it is known that ATP-MgCl2 produces beneficial effects following various adve

44 hexagonal layered CdI2-type structure (beta-MgCl2) at 0.7 GPa: the stacking sequence of the Cl anion

45 flow rate of samples and substrates, buffer, MgCl2, and pH on the detection of E. coli O157:H7 were i

46 erization of un-cross-linked mutant actin by MgCl2 was inhibited strongly but could be restored to wi

47 d faster than rabbit skeletal alpha-actin by MgCl2.

48 Photoinsertion of 8N3ATP was enhanced by MgCl2, independent of the ionic strength, and exhibited

49 oligomers were polymerized into filaments by MgCl2 faster than un-cross-linked actin.

50 at pH 7.4-8.0 and was increased 1.3-fold by MgCl2 (5 mM).

51 tion of 125I-glucagon is decreased 4-fold by MgCl2 and increased 6-fold by Gpp(NH)p.

52 ow-melting-point ternary molten salts CaCl2 -MgCl2 -NaCl, which still retains high CaSiO3 solubility,

53 Magnesium chloride (MgCl2) with the rhombohedral layered CdCl2-type structur

54 aCl)], 2:1 electrolytes [magnesium chloride (MgCl2), calcium chloride (CaCl2)], and electrolyte mixtu

55 lyzed using CRDS, except highly concentrated MgCl2 solutions, without the need for an additional corr

56 tween the unfolded and folded RNA in crowded MgCl2 solutions.

57 e at different temperatures and in different MgCl2 and polyethylene glycol (PEG) concentrations.

58 ally relevant monovalent (KCl) and divalent (MgCl2) cation concentrations.

59 ed bacterial EPS with the addition of either MgCl2 or CaCl2 solution.

60 lm composite polyamide membranes with either MgCl2 or CaCl2 draw solution.

61 Having a non-charge inverting electrolyte (MgCl2) in the other part of the channel and applying an

62 th DNA and an incorrect dNTP, or in elevated MgCl2 concentrations, an intermediate state termed the "

63 ese reasons we suggest that, at the elevated MgCl2 conditions, the RecA-dsDNA nucleoprotein filament

64 i values of 2-11 mM for Na2ATP, 0.2-5 mM for MgCl2, 0.1-5 mM for MgATP, and 20-300 microM for p-nitro

65 A similar trend is observed for MgCl2 which has a CDC value of 1.2 mM MgCl2.

66 be MgX2 >> YCl3 >> AlCl3 and MgI2 > MgBr2 > MgCl2 in every case.

67 on salt type with an overall trend of NaCl > MgCl2 > CaCl2.

68 s, but changes to 6 bp per monomer at higher MgCl2 concentrations, with the transition occurring at a

69 In MgCl2-containing buffer the folding of 12-mer nucleosoma

70 pid nanoparticle-nanoparticle aggregation in MgCl2 solution.

71 nucleosomal array oligomerized completely in MgCl2.

72 bility to form an unstable folded species in MgCl2 whose extent of compaction equals that of canonica

73 By including MgCl2, however, the range of optimal activity was expand

74 hosphatidylserine mole fraction or including MgCl2 in the binding reaction decreases the affinity of

75 uncation of the translocated tRNA, increased MgCl2 concentration, and mutation C2394A of the 50S E si

76 solutions with individual salts (NaCl, KCl, MgCl2, and CaCl2) using deionized water with a known sta

77 interface, for four electrolytes: NaCl, KCl, MgCl2, and CaCl2.

78 me interactions are modulated by salts (KCl, MgCl2) and histone tail deletions (H3, H4 N-terminal), u

79 We studied the influence of membrane lipids, MgCl2, and ATP on the ability of a soluble diacylglycero

80 1.0 mmol/liter compared with 0.5 mmol/liter MgCl2 in myocytes from normal dogs or dogs with heart fa

81 tes were dialyzed with 0.5 or 1.0 mmol/liter MgCl2.

82 The stoichiometry is 3 bp per monomer at low MgCl2 concentrations, but changes to 6 bp per monomer at

83 bitter-tasting compounds: 0.1 M KCl, 0.01 M MgCl2, and 1 mM each phenylthiocarbamide (PTC), L-tyrosi

84 e chips in pH 10 PBS buffer containing 0.1 M MgCl2 increased the electron-transfer resistance for a r

85 A, U1-C, and the Sm core particle) using 1 M MgCl2 or ribonuclease treatment.

86 s C in the presence of 1.0 M NaCl2 and 0.2 M MgCl2 in water.

87 mL/h as the sample flow rate, 1.0 x 10(-2) M MgCl2, and 2.0 x 10(-4) M p-nitrophenyl phosphate in 1.0

88 aCl2), but not by the addition of magnesium (MgCl2).

89 st rechargeable Mg/S battery with a MgTFSI2 /MgCl2 /DME electrolyte (DME=1,2-dimethoxyethane, TFSI=bi

90 5 degrees C, pH 7.4, 125 mM NaCl, and 3.0 mM MgCl2.

91 S (M) and 2.7 pS (L) in 200 mM KCl with 1 mM MgCl2 (intracellular) and 50 mM KCl with no MgCl2 (extra

92 Binding is optimal with 1 mM MgCl2 but decreases with both lower and higher magnesium

93 cal ionic condition of 200 mM KCl (with 1 mM MgCl2 in cis intracellular and 0 MgCl2 in trans extracel

94 Saccharomyces cerevisiae in 50 mM KCl, 1 mM MgCl2, and pH 7.0 buffer at 22 degrees C.

95 At 1 mM MgCl2, both full-length CaM (CaM1-148) and a C-domain fr

96 human nuclei in situ was stabilized by 1 mM MgCl2, but became disrupted in the absence of MgCl2, con

97 hile ATP was saturated at approximately 1 mM MgCl2.

98 , with a Kd of approximately 100 pM at 10 mM MgCl2 (at 37 degrees C).

99 that at a concentration of 60 mM KCl, 10 mM MgCl2 added salt plus minimal neutralizing cations, the

100 In the presence of 10 mM MgCl2 and 10 mM KCl the addition of ATP, but not ADP or

101 association rate constants measured in 10 mM MgCl2 and a binding isotherm measured in 10 mM CaCl2 usi

102 The addition of 10 mM MgCl2 diminished DNA binding to the CRE and PAR DNA sequ

103 oximately 50 nM, while the addition of 10 mM MgCl2 nearly eliminated detectable F-DNA@Ape complexes.

104 The addition of 10 mM MgCl2 to the polyacrylamide gel dramatically altered seq

105 f RNase P holoenzyme to RNase P RNA in 10 mM MgCl2, 100 mM NH4Cl.

106 yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 20 mM glucose (SOC medium) containing 440 mM sorb

107 ps s(-1) (mkUapp=709(+/-14) bp s(-1)) (10 mM MgCl2, 30 mM NaCl (pH 7.0), 5% (v/v) glycerol, 25.0 degr

108 10(7) M-1) (10 mM Tris.HCl, 10 mM KCl, 10 mM MgCl2, 5 mM CaCl2, pH 7.0, 22 degrees C).

109 tep of 1.4 second-1 at 37 degrees C in 10 mM MgCl2, 50 mM NaCl.

110 t 37 degrees C in 20 mM HEPES, pH 7.2, 10 mM MgCl2, and 1 mM spermine.

111 , CysB(W166R), was totally inactive at 10 mM MgCl2, but gave constitutive expression of the cysK and

112 with in vivo behaviour at 2 mM than at 10 mM MgCl2, suggesting that the former is the more physiologi

113 At 10 mM MgCl2, the in vitro transcription activity of CysB(T149M

114 reen agarose per ml in the presence of 10 mM MgCl2.

115 in 10 versus 200 mM NaCl at a constant 10 mM MgCl2.

116 cysK promoter at 2 mM mgCl2 but not at 10 mM MgCl2; it required inducer for cysJIH promoter activatio

117 At 125 mM MgCl2, Kd = 0.40 +/- 0.05 mM.

118 hosphate buffer, pH 7.2/0.5 mM CaCl2/0.15 mM MgCl2 (MBC50 range, 2.8-11.5 mM).

119 ining 0.14 M NaCl, 0.5 mM CaCl2, and 0.15 mM MgCl2 or with this buffer and either 25% heat-inactivate

120 degrees C, in 50 mM Hepes (25 mM Na+), 15 mM MgCl2, and 135 mM KCl at pH 7.5, the exogenous 5' exon m

121 of tRNAPhe at 25 degrees C, pH 7.0 and 15 mM MgCl2, Zn2 HIV-1 NC proteins which lack one or both flex

122 S (symmetrical solutions of 150 mM KCl, 2 mM MgCl2 and 2 mM EGTA).

123 nzyme has a pH optimum of 9.0, requires 2 mM MgCl2 for maximum activity and retains full enzyme activ

124 Addition of 2 mM MgCl2 resulted in a 30% decrease in the maximum emission

125 ded and highly folded conformations in <2 mM MgCl2, and self-associate above 2 mM MgCl2.

126 At 2 mM MgCl2, both proteins were fully active without inducer.

127 40S or the 55S folded conformations in 2 mM MgCl2.

128 ion of the cysK and cysJIH promoters at 2 mM MgCl2.

129 n <2 mM MgCl2, and self-associate above 2 mM MgCl2.

130 ed for MgCl2 which has a CDC value of 1.2 mM MgCl2.

131 d type tolerated the addition of > or =20 mm MgCl2 to the culture medium before growth was slowed and

132 In 20 mM MgCl2, many tertiary interactions appeared within 20 ms.

133 .1 angstroms resolution at pH 9.2 with 20 mM MgCl2.

134 Mg2+concentration (97% yield, pH 7.5, 200 mM MgCl2, 25 degreesC).

135 ive to the linear template at pH 7.5, 200 mM MgCl2, 4 degreesC.

136 on the linear template was at pH 6.0, 200 mM MgCl2, 4 degreesC.

137 t optimal ionic strength (1.0 M NH4Cl, 25 mM MgCl2), deletion or substitution of the 3'-proximal C re

138 ntrations of KCl and MgCl2; addition of 3 mM MgCl2 to 100 mM KCl changes the rip force of Mal from 21

139 (ph 7.4, 25 degrees C, 120 mM NaCl, and 3 mM MgCl2).

140 d to be 44 mM NaCl, 0.9 mM CaCl2, and 1.3 mM MgCl2.

141 respectively, at pH 8.5, 150 mM NaCl, 30 mM MgCl2, and saturating NAD+.

142 In >/=4 mM MgCl2, however, both fully trypsinized arrays and each h

143 ange at 10 degrees C in 100 mM NaCl and 5 mM MgCl2 at pH 5.5 and in the presence of two exchange cata

144 ation rate constant decreases 7-fold at 5 mM MgCl2 for the peptide KKKSKTKCVIM (C-terminal sequence o

145 (in 100 mM NaCl) and RNA hairpins (in 2.5 mM MgCl2) are similar despite differences in the salt condi

146 ons (pH 8.1, 10 degrees C, 100 mM NaCl, 5 mM MgCl2) by the intrinsic binding constant K = 6 +/- 2 x 1

147 e used (4 degrees C, pH 7.5, 6 mM NaCl, 5 mM MgCl2, 10 % (v/v) glycerol).

148 P2S2 (20 mM Tris-HCl, pH 7.5, 6mM NaCl, 5 mM MgCl2, 10% glycerol, 4 degrees C).

149 -1 s-1 [20 mM Tris (pH 7.5), 6 mM NaCl, 5 mM MgCl2, 5 mM 2-mercaptoethanol, and 10% (v/v) glycerol, 4

150 s-1 [20 mM Tris-HCl, pH 7.5, 6 mM NaCl, 5 mM MgCl2, 5 mM 2-mercaptoethanol, and 10% (v/v) glycerol, 4

151 e binds to AB-APoppin the presence of 2.5 mM MgCl2, a 3-fold decrease in fluorescence is observed ( K

152 ding without GEP was much lower; with 2-5 mM MgCl2, GEP-stimulated binding was maximal.

153 200 min at 90 (o)C in the presence of 0.5 mM MgCl2, suitable for simultaneous starch gelatinization a

154 In 2.5 mM MgCl2, the stabilities of both ssDNA and RNA hairpins sc

155 ptimal divalent-cation concentration of 5 mM MgCl2.

156 e transition occurring at approximately 5 mM MgCl2.

157 t of 9 microM in 22.5 mM Hepes, pH 7.0, 5 mM MgCl2.

158 Resolvase activity is optimal with 5 mM MgCl2.

159 ial centrifugation over the range of 0-50 mm MgCl2 to determine how each NTD affects salt-dependent o

160 Pseudo-origin denaturation occurred in 7 mM MgCl2, distinguishing this reaction from Mg2+-independen

161 In the presence of 0.6-0.8 mM MgCl2 and 1 mM EDTA, binding of guanosine 5'-[gamma[35S]

162 ntaining 100 mM potassium glutamate and 8 mM MgCl2 or 10 mM Tris-HCl and 200 mM KCl, with or without

163 reconstitution conditions [350 mM KCl, 8 mM MgCl2, and 30 mM Tris (pH 7.5)], a complex with an assoc

164 the assay, approaching saturation at 5-8 mM MgCl2, while ATP was saturated at approximately 1 mM MgC

165 salt conditions (0.1 M NH4Cl and several mM MgCl2) was shown by its following properties: (i) an unu

166 as reduced in the presence of MgATP, Na2ATP, MgCl2, 2',3'-O-(2,4, 6-trinitrophenyl)-ATP, and p-nitrop

167 stabilization effect) and salts (LiCl, NaCl, MgCl2, and CaCl2).

168 f aquatic chemistries (pH, salt types (NaCl, MgCl2, CaCl2), ionic strength) relevant to natural and e

169 in DNA helical repeat, gamma, in mixed NaCl/MgCl2 solutions.

170 P]/[E] = 1.7) in the presence of excess NaF, MgCl2, and poly(rU).

171 MgCl2 (intracellular) and 50 mM KCl with no MgCl2 (extracellular), with pH maintained at 7.4 by 10 m

172 containing DNA in the presence or absence of MgCl2 and ATP and formed complexes resistant to heat ina

173 ng was assayed in the presence or absence of MgCl2 and/or KCl using two methods: circular dichroism (

174 ity was 200-300-fold lower in the absence of MgCl2, but the Km was only slightly affected.

175 gCl2, but became disrupted in the absence of MgCl2, conditions that also dissociated the oligomers in

176 8C mutant in the Mg2+ ATP state (addition of MgCl2 + 5'-adenylyl-beta,gamma-imidodiphosphate).

177 entially when Mg2+ and ADP + Pi (addition of MgCl2 + ATP) was present, while the alpha-epsilon cross-

178 ertiary structure formation upon addition of MgCl2 are also site specific, with local conformational

179 orescence intensity changes upon addition of MgCl2 were monitored over a time-course from 1ms to 100s

180 times larger than that with the addition of MgCl2.

181 but no change was observed after addition of MgCl2.

182 A small amount of MgCl2 added to the DNA suspension increased the DNA-surf

183 the addition of millimolar concentrations of MgCl2 or CaCl2 to the trans solution.

184 in the presence of various concentrations of MgCl2 solution with total internal reflection fluorescen

185 ve PCR instrumentation, 19,400 conditions of MgCl2, ligand and temperature are analysed to generate d

186 Specific effects of MgCl2 were observed for mutants K53A, R93Q and R93E.

187 Moreover, inclusion of MgCl2, substitution of KCl for NaCl, or alterations in t

188 Millimolar levels of MgCl2 reportedly act synergistically with NaCl to produc

189 CSP41 is active only in the presence of MgCl2 and CaCl2.

190 rmal actin polymerization in the presence of MgCl2 and phalloidin.

191 ased on heat inactivation in the presence of MgCl2 did not completely inactivate SV40.

192 The presence of MgCl2 in solutions appears to considerably elongate the

193 r stability over time and in the presence of MgCl2 salt is demonstrated.

194 maximal binding occurred (in the presence of MgCl2) when the vesicles contained anionic phosphoglycer

195 esented here suggest that in the presence of MgCl2, Ogg1 can efficiently process 8-oxoguanine so as t

196 om the wild type receptor in the presence of MgCl2.

197 ciated with the membranes in the presence of MgCl2.

198 the conventional thermal cycler at optimized MgCl2 concentration.

199 tion of ATPase activity with F- (n = 2.8) or MgCl2 (n = 2.1) in the presence of excess ADP and poly(r

200 0 mM phosphate, pH 7.0) and NaCl, KCl and/or MgCl2 added to different concentrations in order to eval

201 e reduced by the addition of 1-5 mM BaCl2 or MgCl2 to the lumenal side, which contained 50 microM Ca2

202 in the presence of additional salts (NaCl or MgCl2) shows that toroid thickness is a salt-dependant p

203 estored by FeSO4 but not by CuSO4, ZnCl2, or MgCl2.

204 ium metal by magnesium metal; the by-product MgCl2 is removed by vacuum distillation.

205 GEP activity required MgCl2.

206 This required MgCl2 and a hydrolyzable form of ATP and was prevented b

207 The reaction requires MgCl2 and is inhibited by inorganic phosphate, which is

208 According to our experimental results MgCl2 remains in a 2D layered phase up to 100 GPa and fu

209 erved in 50 mM solutions of magnesium salts (MgCl2 and MgSO4).

210 formation of three commonly prevalent salts, MgCl2, CaCl2, and NaCl, next to a sapphire substrate usi

211 CaCl2 destabilized GO more aggressively than MgCl2 and NaCl due to the binding capacity of Ca(2+) ion

212 non-charge inverting electrolyte other than MgCl2, either this electrolyte or the Ru(bpy)3Cl2 soluti

213 abilization is weaker in NaCl solutions than MgCl2 solutions.

214 In contrast, the MgCl2 and CaCl2 surface hydrate crystals are interdisper

215 mM); the monovalent salt also inhibited the MgCl2-induced polymerization of actin.

216 er, the binding stoichiometry depends on the MgCl2 concentration.

217 e cratic free energies of (re)organizing the MgCl2 and NaCl atmospheres, DeltaG(Mg)org and DeltaDelta

218 Activity was directly correlated to the MgCl2 concentration and inversely correlated to the KCl

219 Above this MgCl2 concentration, the dsDNA within the RecA nucleopro

220 mM KCl to the gels had an effect similar to MgCl2.

221 demonstrate that solar cells prepared using MgCl2, which is non-toxic and costs less than a cent per

222 more than 100-fold increase in the rate when MgCl2 was included in the reaction.

223 ectrochemical dichlorination of alkenes with MgCl2 as the chlorine source.

224 lpha; however, upon activation of alpha with MgCl2 and GTPgammaS under nondenaturing conditions, the

225 t CdCl2 may simply be replaced directly with MgCl2 in the existing fabrication process, thus both min

226 -15N HSQC titrations of the free enzyme with MgCl2 followed by equimolar AMPCPP/MgCl2.

227 HSQC titrations of the wild type enzyme with MgCl2 show changes in chemical shifts of 15N and NH reso

228 ere treated with CaCl2, while treatment with MgCl2 had no effect.

229 s for the reactions in LiCl with and without MgCl2 were both bell-shaped with the pH optima in the ne