ライフサイエンスコーパス: MoMLV

1 MoMLV Gag contains two additional L domains, PSAP and LY

2 All MoMLV-inoculated mice produced ecotropic viruses that re

3 titers on M. dunni and SC-1 cells, although MoMLV does not efficiently infect M. dunni cells.

4 ture prediction suggested that the HIV-1 and MoMLV peptides could form 50% and 38% alpha-helix, respe

5 ent research has demonstrated that HIV-1 and MoMLV targeting preferences are in large part guided by

6 hanistic studies, we show for both HIV-1 and MoMLV that each HMG I(Y) monomer must contain multiple D

7 d-through depends on the interaction between MoMLV RT and peptidyl release factor 1 (eRF1).

8 Promotion of read-through by MoMLV RNase H prevents nonsense-mediated mRNA decay (NMD

9 XPK2) that are not efficiently transduced by MoMLV-based vectors pseudotyped with many different vira

10 e show that the variation of transduction by MoMLV-based vectors in mammalian and nonmammalian cells

11 Itch-mediated rescue of release-defective MoMLV was sensitive to inhibition by dominant-negative v

12 ngly, Itch stimulation of the PPPY-deficient MoMLV release was accompanied by the enhancement of Gag

13 iled to rescue the release of PPPY-deficient MoMLV via these other L domains.

14 ed cell-free plasma viremia and also delayed MoMLV-induced disease.

15 that do not contain the previously described MoMLV DLS.

16 To this end, MoMLV Gag, Gag-Pol, and gRNA were expressed separately o

17 ese results suggest that Itch can facilitate MoMLV release in an L domain-independent manner via a me

18 rs (LEDGF/p75 for HIV-1 and BET proteins for MoMLV) that tether viral intasomes to chromatin.

19 Thymus and spleen cells from MoMLV-inoculated mice were plated on Mus dunni cells and

20 ence of a representative clone differed from MoMLV by insertion of two serine residues within the VRA

21 l phenotypes distinguished the isolates from MoMLV.

22 hibited by tetherin and is required for full MoMLV pathogenesis.IMPORTANCE Retroviruses are thought t

23 ur results provide a mechanistic view of how MoMLV manipulates the host translation termination machi

24 In MoMLV, a hairpin loop functioning as a dimer linkage str

25 were inoculated with Moloney ecotropic MLV (MoMLV).

26 pseudotypes having AKV Env and Moloney MLV (MoMLV) Gag proteins, further indicating that AKV Env seq

27 es, we inoculated neonates with Moloney MLV (MoMLV) or amphotropic MLV (A-MLV) and screened for viral

28 (LTR) promoters-murine sarcoma virus (MSV), MoMLV (MLV), and the LTR (termed Rh-MLV) that is derived

29 Analysis of MoMLV-AKV MLV chimeras determined that the target of res

30 Modification of a molecular clone of MoMLV by the addition of these serines produced a virus

31 A comparison of MoMLV-based vector transduction in mammalian and nonmamm

32 e show that BAF is an authentic component of MoMLV.

33 indicating that Itch-mediated correction of MoMLV release defects requires the integrity of the host

34 Our results show that dimerization of MoMLV RNA can be nucleated at multiple sites and suggest

35 cleus to cytoplasm while the distribution of MoMLV integrase was unaffected.

36 ently rescued the release and infectivity of MoMLV lacking PPPY function.

37 is domain, we were able to divide the NTD of MoMLV CA into three functional regions.

38 lls had little or no effect on the number of MoMLV-infected splenocytes and thymocytes.

39 cells resulted in the specific reduction of MoMLV cell-free plasma viremia but not the number of inf

40 hese cells do not support the replication of MoMLV, and cells from A-MLV-inoculated mice were plated

41 of Rh-MLV LTR and a partial gag sequence of MoMLV (Deltagag(871-1612)) in CS-Rh-MLV-E gave the highe

42 Here, we report the crystal structure of MoMLV RT in complex with eRF1.

43 wever, pTP-mediated nuclear translocation of MoMLV DNA in nondividing cells was not sufficient for st

44 nd that HMG I(Y) can condense model HIV-1 or MoMLV cDNA in vitro as measured by stimulation of interm

45 firmed the superiority of HIV-1 vectors over MoMLV vectors for gene transfer into canine bone marrow

46 Additionally, VSV-G-pseudotyped MoMLV-based vector transduction is attenuated in the zeb

47 PY motif and to be incorporated into rescued MoMLV particles.

48 d LYPAL, that are believed to drive residual MoMLV release via interactions with cellular proteins Ts

49 cribed a Moloney murine leukemia retroviral (MoMLV) vector useful for the generation of anchored long

50 lar stomatitis virus (VSV) or the retrovirus MoMLV.

51 On the basis of the assumption that MoMLV evolved to produce virion components in optimal pr

52 this issue of Cell, Orlova et al. show that MoMLV reverse transcriptase binds the translation releas

53 Overall, these results suggest that MoMLV spread within hematopoietic tissues and cell monol

54 The MoMLV RT interacts with the C-terminal domain of eRF1 vi

55 ging cells that express the JSRV Env and the MoMLV Gag-Pol proteins and can produce JSRV-pseudotype v

56 cribe RNA dimers formed from sections of the MoMLV 5' untranslated region that do not contain the pre

57 Itch suppressed the residual release of the MoMLV lacking the PPPY motif.

58 All of the MoMLV-inoculated mice also produced nonecotropic virus t

59 structure over half of its length, while the MoMLV peptide is over one third alpha-helix.

60 duced ecotropic viruses that resembled their MoMLV progenitor, although some isolates, unlike MoMLV,

61 Sequencing of three MoMLV- and two A-MLV-derived nonecotropic recombinants c

62 V progenitor, although some isolates, unlike MoMLV, grew to high titers in M. dunni cells.

63 ns of estimating the proportion of unspliced MoMLV RNA that serves as genomic RNA.

64 I (HIV-1) and Moloney murine leukemia virus (MoMLV) capsid proteins were investigated by several tech

65 1 (HIV-1) and Moloney murine leukemia virus (MoMLV) cDNA.

66 mmaretrovirus Moloney murine leukemia virus (MoMLV) favors strong enhancers and active gene promoter

67 Moloney murine leukemia virus (MoMLV) Gag utilizes its late (L) domain motif PPPY to bi

68 us (FIV), and Moloney murine leukemia virus (MoMLV) integrases were stably expressed to determine the

69 ptase (RT) of Moloney murine leukemia virus (MoMLV) is expressed in the form of a large Gag-Pol precu

70 ntegration of Moloney murine leukemia virus (MoMLV) PICs in vitro.

71 his domain in Moloney murine leukemia virus (MoMLV) replication, we analyzed 18 insertional mutations

72 The Moloney murine leukemia virus (MoMLV) ribonucleoprotein complex is composed of an appro

73 H function of Moloney Murine Leukemia Virus (MoMLV) RT and also inhibited Escherichia coli RNase H.

74 es not affect Moloney murine leukemia virus (MoMLV) spread, and only minimally affects vesicular stom

75 fficient than Moloney murine leukemia virus (MoMLV) vectors for transduction of canine bone marrow mo

76 ly similar to Moloney murine leukemia virus (MoMLV), another gammaretrovirus.

77 life cycle of Moloney murine leukemia virus (MoMLV), we analyzed the intracellular complexes mediatin

78 contrast to a Moloney murine leukemia virus (MoMLV)-based retrovirus vector (SRalphaLEGFP).

79 to pseudotype Moloney murine leukemia virus (MoMLV)-based retrovirus vectors and that such vectors ca

80 uggested that Moloney murine leukemia virus (MoMLV)-based vectors pseudotyped with the vesicular stom

81 Moloney murine leukemia virus (MoMLV)-derived vectors require cell division for efficie

82 the genome of Moloney murine leukemia virus (MoMLV).

83 at indicate that HMG I(Y) is associated with MoMLV preintegration complexes.

84 lly, we found Itch to immunoprecipitate with MoMLV Gag lacking the PPPY motif and to be incorporated

85 es and linear viral DNA after infection with MoMLV but not with AKV MLV.