ライフサイエンスコーパス: N-glycosidase F

1 y enzymatic treatment with Endoglycosidase-F/N-glycosidase-F).

2 imately 160 kDa after treatment with peptide N-glycosidase F.

3 a more rapid migration after treatment with N-glycosidase F.

4 wing release from the polypeptide by peptide-N-glycosidase F.

5 ly inhibited by deglycosylation with peptide N-glycosidase F.

6 glycosylation with endoglycosidase F/peptide-N-glycosidase F.

7 d migration in SDS-PAGE after treatment with N-glycosidase F.

8 pus oocytes and deglycosylation with peptide-N-glycosidase F.

9 type VIII-B is insensitive to treatment with N-glycosidase F.

10 Moreover, treatment with peptide N-glycosidase F abrogated F240 neutralization, in an iso

11 nosaccharides based on resistance to peptide-N-glycosidase F and analysis of saccharides released by

12 Controlled deglycosylation using peptide : N-glycosidase F and endo-beta-N-acetylglucosaminidase F3

13 immunoprecipitated TPP I proenzyme with both N-glycosidase F and endoglycosidase H as well as treatme

14 Peptide N-glycosidase F and endoglycosidase H deglycosylate hENT

15 Deglycosylation using peptide-N-glycosidase F and site-directed mutagenesis establishe

16 with Flavobacterium meningosepticum peptide N-glycosidase F and trypsin, with matrix-assisted laser

17 approaches, including use of either peptide:N-glycosidases F and A (PNGase F and A) or anhydrous hyd

18 NRP-2 is resistant to digestion with peptide N-glycosidase F but is sensitive to release under alkali

19 Peptide N-glycosidase F, but not endoglycosidase H, digestion co

20 Treatment with N-glycosidase F confirmed that beta3 is a glycoprotein c

21 ely 120 kDa following treatment with peptide:N-glycosidase F, consistent with N-glycosylation being t

22 Treatment with N-glycosidase F converted both bands to smaller molecula

23 sylation of PHF tangles by endoglycosidase F/N-glycosidase F converts them into bundles of straight f

24 actosyltransferase or susceptible to peptide N-glycosidase F corresponded directly to their relative

25 ng mass spectrometry on purified and peptide N-glycosidase F-deglycosylated CD36 and also by comparin

26 Metabolic labelling of cells and N-glycosidase F digestion of cell extracts indicated tha

27 atment during receptor biosynthesis, but not N-glycosidase F digestion of mature receptors, abrogated

28 Peptide-N-glycosidase F digestion of solubilized hKOR or incubat

29 N-deglycosylation by peptide-N-glycosidase F digestion resolved these isoforms into t

30 oglycosidase F or H but sensitive to peptide N-glycosidase F digestion.

31 by mutagenesis, as substantiated by protein N-glycosidase F digestions and Western immunoblotting, d

32 ing sequential endoglycosidase H and peptide:N-glycosidase-F digestions.

33 after digestion with high concentrations of N-glycosidase F, endoglycosidase F, endoglycosidase H, a

34 Digestion with N-glycosidase F, endoglycosidase H and lectin blotting d

35 d: Route A, lysyl endopeptidase C, then endo-N-glycosidase F, followed by cyanogen bromide; Route B,

36 lly released from glycoproteins with peptide N-glycosidase F, followed by purification with graphitiz

37 ese hamster ovary cells, deglycosylated with N-glycosidase F, forms a 80-90 kDa complex with mature T

38 ses of Man(6)GlcNAc(2) released with peptide-N-glycosidase F from invertase secreted by Deltaalg9 yea

39 r a simple and fast incubation using peptide-N-glycosidase F on target, sequential mass shifts were o

40 Pretreatment of the lactoferrin with peptide N-glycosidase F or addition of heparin or chondroitin su

41 of the oligosaccharide chains using peptide N-glycosidase F or removal of the glucoses by ER glucosi

42 lated species and was converted to 27 kDa by N-glycosidase F or tunicamycin treatments.

43 gnized by the classical glycosidases peptide-N-glycosidase F (PNGase F) and endoglycosidase H (Endo H

44 cities of endoglycosidases such as a peptide-N-glycosidase F (PNGase F) and of endo-N-acetlyglucosami

45 es in electrophoretic mobility after peptide-N-glycosidase F (PNGase F) digestion suggest that both P

46 de mapping, bands were digested with peptide N-glycosidase F (PNGase F) in order to release the N-lin

47 online enzyme reactor incorporating peptide-N-glycosidase F (PNGase F) on a monolithic polymer suppo

48 Deglycosylation with protein-N-glycosidase F (PNGase F) reduced the size of both lung

49 odies, dual digestion by trypsin and peptide-N-glycosidase F (PNGase F), and analysis by LC-MS/MS.

50 N-linked glycosylated peptides via peptide- N-glycosidase F (PNGase F).

51 cted with GPVI, deglycosylation with peptide-N-glycosidase F (PNGase F; specific for complex N-linked

52 brane preparations were treated with peptide N-glycosidase F (PNGase-F).

53 A deglycosylation step using Peptide-N-Glycosidase F (PNGaseF) has been introduced in a stand

54 Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), directly on tissues followed

55 Treatment with peptide-N-glycosidase F reduced the size of the mammalian cell-

56 are glycosylated, and treatment with peptide N-glycosidase F reduces the apparent molecular mass on S

57 t of AGMK and cr5 cell extracts with peptide-N-glycosidase F resulted in the collapse of the havcr-1-

58 Deglycosylation of the receptor with peptide N:glycosidase F results in a decrease in molecular mass

59 Pretreatment with N-ethylmaleimide and N-glycosidase F revealed an AC IV band at 115 kDa.

60 Treatment of heart lysates with peptide-N-glycosidase F revealed that while giant obscurin-B loc

61 o-beta-N-acetylglucosaminidase H and peptide:N-glycosidase F sensitivity assays on CDKAL1 constructs

62 ecular masses in each system, treatment with N-glycosidase F shifted all proteins to a molecular mass

63 ent of kidney membrane proteins with peptide N-glycosidase F showed that GLUT9 and GLUT9DeltaN are ex

64 and was sensitive to treatment with peptide N-glycosidase F, sialidase alone, or sialidase and O-gly

65 the cultured medium, and upon treatment with N-glycosidase F, the molecular mass was lowered by appro

66 -Ser/Thr) glycosylation, a lack of effect of N-glycosidase F, the presence of 70 and 126 Ser/Thr glyc

67 from the antibody via digestion with peptide-N-glycosidase F, then derivatized with a charged fluorop

68 Etanercept was first treated with peptide N-glycosidase F to release the N-glycans.

69 d bound fractions, and treated with peptide: N-glycosidase F to remove N-linked glycans.

70 enzyme could be deglycosylated with peptide N-glycosidase-F to a core molecular mass of 54 kDa.

71 rotein-tagged mCLCA6 with PNGase F (peptide: N-glycosidase F) to remove N-linked glycosyl groups show

72 lycosylation was responsible because peptide N-glycosidase F treatment of isolated 170-kDa EGFR yield

73 polyacrylamide gel electrophoresis and after N-glycosidase F treatment revealed that extensive glycos

74 N-Glycosidase F treatment shows that the alternative pro

75 ycoproteins and molecular mass after peptide-N-glycosidase F treatment was 38 and 45 kDa, respectivel

76 ied Thy-1 in an ELISA, and by sensitivity to N-glycosidase-F treatment.

77 ed CCR2B was found to be N:-glycosylated, as N:-glycosidase F treatment of the receptor or growth of

78 th trifluoromethanesulfonic acid and peptide-N-glycosidase F treatments yielded a 50-kDa band, indica

79 as examined by using tunicamycin and peptide N-glycosidase F, two agents used to prevent and remove g

80 n of the full-length homotrimer with peptide N-glycosidase-F under native conditions abolished recogn

81 ells with trypsin, endoglycosidase F/peptide N-glycosidase F, Vibrio cholerae neuraminidase, tunicamy

82 h N-linked carbohydrate was removed by using N-glycosidase F was markedly less effective in protectin

83 Treatment of ERbeta with N-glycosidase F, which removes core carbohydrate residue