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1                                              N. caninum DNA was amplified most consistently from brai
2                                              N. caninum expressing TgGRA15 differentially disturbed t
3                                              N. caninum-infected mice produced significantly higher l
4 onoclonal antibody (MAb), MAb 4A4-2, against N. caninum tachyzoites.
5 he mechanisms involved in protection against N. caninum-associated abortions.
6 rearrangements had occurred in T. gondii and N. caninum since their most recent common ancestry.
7 cow sera defined by fetal histopathology and N. caninum immunohistochemistry and by maternal N. canin
8 caninum tachyzoite lysate antigen (NcAg) and N. caninum tachyzoite culture supernatant.
9 ng inoculation consisting of live, avirulent N. caninum tachyzoites followed by virulent challenge du
10 e of exposure of polar bears to C. burnetii, N. caninum, and F. tularensis.
11 cows with abortion confirmed to be caused by N. caninum.
12 rgeted genes of Toxoplasma gondii, driven by N. caninum promoters, have yielded robust expression and
13                                   In cattle, N. caninum has particular significance as a cause of abo
14    Abundant NcCyP was detected in whole-cell N. caninum tachyzoite lysate antigen (NcAg) and N. canin
15                            PCR also detected N. caninum DNA in 6 of 8 fetuses that had typical lesion
16     In fresh or frozen tissues, PCR detected N. caninum DNA in 10 of 13 true-positive fetuses (77%) a
17 ixed paraffin-embedded tissues, PCR detected N. caninum DNA in 13 of 13 true-positive fetuses (100%)
18  we examined the utility of PCR in detecting N. caninum infection in fetal tissues from spontaneous b
19 d to analyze several independent and diverse N. caninum isolates; both antigens were recognized in al
20 eptor-mediated apoptosis is repressed during N. caninum infection, and the data further showed that t
21 gnizes several pathogens and its role during N. caninum infection has not yet been described.
22 ons that coyotes (Canis latrans) can excrete N. caninum oocysts in their feces and that white-tailed
23 es that Nod2-dependent responses account for N. caninum elimination.
24               Current diagnostic methods for N. caninum infection in cattle and the advances necessar
25                                         Four N. caninum-infected Holstein cattle developed NcSRS2 pep
26 signated Ncp29 and Ncp35, respectively) from N. caninum tachyzoites that are the predominant antigens
27 ive transfer of CD8+ T-cell splenocytes from N. caninum-infected mice was protective against challeng
28                           On the other hand, N. caninum expressing TgROP16 induced host STAT3 phospho
29  and versatile method to accurately identify N. caninum infection status in cattle using a single cut
30                                           In N. caninum tachyzoite culture supernatant, three NcCyP b
31 tive IkappaB kinase activity was detected in N. caninum extracts, thereby implying that this parasite
32 he NF-kappaB subunit p65 was not detected in N. caninum-infected cells, although this host transcript
33 ndicate that heterologous gene expression in N. caninum is a useful tool for the study of specific ge
34  the reactive oxygen species (ROS) levels in N. caninum tachyzoites.
35 tor subsets indicated that CD4(+) CTL killed N. caninum-infected, autologous target cells and that ki
36 caninum immunohistochemistry and by maternal N. caninum indirect fluorescence assay (IFA) at a 1:200
37                     In summary, the modified N. caninum cELISA provided a simple, rapid, and versatil
38 oss-reactive antibodies recognizing multiple N. caninum antigens by immunoblot assay, did not inhibit
39 ions to the cELISA included capturing native N. caninum antigen with a parasite-specific MAb (MAb 5B6
40 paB pathway is not central to the ability of N. caninum to prevent apoptosis of their host cells.
41                       Immunoblot analysis of N. caninum tachyzoite antigens with sera from cows with
42                              Confirmation of N. caninum infection by immunohistochemistry has low sen
43                             PCR detection of N. caninum DNA in formalin-fixed, paraffin-embedded tiss
44  latitudinal gradient, with the exception of N. caninum.
45 or 11 (TLR11), but the ectopic expression of N. caninum profilin in T. gondii had no impact on early
46 stance indices resolved a single genotype of N. caninum Whole-genome sequencing of 7 isolates from 2
47 n 6 of 8 fetuses that had typical lesions of N. caninum but were immunohistochemistry negative, indic
48 histochemistry determined the true status of N. caninum infection in each fetus.
49 2 bound diffusely to the exterior surface of N. caninum tachyzoites and recognized a single 65-kDa ba
50 was inhibited by mild periodate treatment of N. caninum antigen, demonstrating the carbohydrate natur
51 ne Blue (DMMB) and Toluidine Blue O (TBO) on N. caninum, using in vitro and in vivo models.
52  strains of T. gondii and its near relative, N. caninum We significantly improved T. gondii genome co
53 4-2 binds a carbohydrate epitope on a single N. caninum tachyzoite surface antigen that is recognized
54                               In this study, N. caninum-specific CTL expanded from peripheral blood m
55 se findings support investigation of subunit N. caninum vaccines incorporating NcSRS2 gene sequences
56 FN-gamma(-/-) mice, MyD88(-/-) mice survived N. caninum infections at the dose used in this study.
57 crobial protein in the N. caninum tachyzoite N. caninum cyclophilin (NcCyP) as a major component of t
58          These observations demonstrate that N. caninum protects against lethal T. gondii infection b
59 ing this approach, we here demonstrated that N. caninum expressing T. gondii's GRA15 and ROP16 kinase
60 of these parasites and present evidence that N. caninum and H. heydorni are separate species.
61 dy has identified a microbial protein in the N. caninum tachyzoite N. caninum cyclophilin (NcCyP) as
62 e (PVM) of T. gondii was not apparent on the N. caninum PVM.
63              These results indicate that the N. caninum tachyzoite naturally produces a potent IFN-ga
64                  The binding of MAb 4A4-2 to N. caninum tachyzoite antigen was consistently inhibited
65  the viruses, but higher odds of exposure to N. caninum and T. gondii; the opposite was true for wolv
66 ar apicomplexan protozoan closely related to N. caninum.
67 We compared mouse innate immune responses to N. caninum and T. gondii and found marked differences in
68 ion and characterization of CTL responses to N. caninum in the natural, outbred, bovine host will fac
69 hat MyD88(-/-) mice were more susceptible to N. caninum infections than wild-type (WT) mice, and cont
70 decrease in the numbers of mice transmitting N. caninum and a lower frequency of transmission by indi
71 parasite-specific CTL against transplacental N. caninum transmission in cattle.
72  Testing of the 4,323 bovine sera of unknown N. caninum status revealed a distinct bimodal distributi
73 lone, and a set of 4,323 cow sera of unknown N. caninum status.
74 e most widely distributed pathogens, whereas N. caninum was relatively uncommon.
75 ent study was conducted to establish whether N. caninum is similarly capable of subverting apoptotic
76 uring the first hours after infection, while N. caninum is not, and this is likely due to the early M
77 P38 Finally, when we compared T. gondii with N. caninum, we found that although the 13-chromosome kar
78  high degree of resistance to infection with N. caninum.
79 6) that were infected intraperitoneally with N. caninum were protected against a lethal challenge fro
80 lation in mice that had been vaccinated with N. caninum and challenged with T. gondii was observed.