ライフサイエンスコーパス: N. crassa

1 N. crassa H3K27me3-marked genes are less conserved than

2 N. crassa is considerably more morphologically and devel

3 N. crassa is of course a saprophyte and there is no comp

4 N. crassa is typically found on woody biomass and is com

5 ed the growth defect characteristic of dim-5 N. crassa but did not fully rescue the gross DNA hypomet

6 A N. crassa mutant carrying deletions for both transporter

7 A screen of a N. crassa transcription factor deletion collection ident

8 Previously, we have shown that a N. crassa mutant carrying deletions of three beta-glucos

9 In addition, N. crassa nitrite reductase displays several partial act

10 Additionally, N. crassa struggled to degrade any wood substrate (< 6%

11 tDef4 against F. graminearum but not against N. crassa.

12 form a nutrient sensing network that allows N. crassa cells to regulate nitrogen utilization.

13 fourth component, Neurospora protein 55 (an N. crassa homolog of p55/RbAp48), is critical for H3K27m

14 hat the nonreducing end product formed by an N. crassa PMO is a 4-ketoaldose.

15 Here, we show that an N. crassa mutant carrying deletions of two genes encodin

16 of the AAP and the RNA encoding it using an N. crassa cell-free translation system.

17 We then analyzed N. crassa and Schizosaccharomyces pombe telomerase recon

18 H3K9 in both mouse embryonic stem cells and N. crassa.

19 ollowing the divergence of S. cerevisiae and N. crassa and provides insight into the evolution of kin

20 ) of mtDNA from Sc. pombe, S. cerevisiae and N. crassa, but bands without smears were found for diges

21 Both the A. niger and N. crassa proteins show homology with a stress-inducible

22 these chromosomes between N. tetrasperma and N. crassa.

23 he type exhibited by related species such as N. crassa.

24 This suggests that the widespread and basal N. crassa-type spliceosomal cleavage mechanism is more a

25 overall rate of molecular evolution between N. crassa and S. cerevisiae were not detected.

26 itation experiments, a heterocomplex between N. crassa HET-C1 and PhcA was associated with phcA-induc

27 d from a favored carbon source to cellulose, N. crassa dramatically up-regulates expression and secre

28 carbon source such as sucrose to cellulose, N. crassa dramatically upregulates expression and secret

29 Unlike S. cerevisiae, N. crassa has a single isoform of the a subunit.

30 ing translation extracts from S. cerevisiae, N. crassa, and wheat germ.

31 reated mutants of each of the four classical N. crassa HDAC genes and tested their effect on histone

32 was able to attach and extensively colonize N. crassa hyphae, while an Escherichia coli control show

33 We have developed N. crassa as a model system where we can dissect the com

34 size in linear form for all species (except N. crassa) and in multi-fibered, comet-like forms for mo

35 We assessed this adaptation for N. crassa by quantifying the decay of wood and grasses t

36 functional genomics resources available for N. crassa, which include a near-full genome deletion str

37 ne densities (12,000-13,000 total genes) for N. crassa.

38 -decay, support a fire-response strategy for N. crassa, rather than a fire-adaptive ability to degrad

39 A cell-free N. crassa system was developed that required the presenc

40 Analyses of purified QDE-1 polymerases from N. crassa (QDE-1(Ncr)) and related fungi, Thielavia terr

41 upon deletion of Puf4 in filamentous fungi (N. crassa) in contrast to the increase upon Puf3 deletio

42 inhibition (toeprint) assay in a homologous N. crassa cell-free translation system showed that argin

43 btained by translating mRNAs in a homologous N. crassa in vitro translation system or in rabbit retic

44 cosmids, on average, contained an identified N. crassa gene sequence as a starting point for gene ide

45 In N. crassa the mutant strains did not exhibit reduced spo

46 In N. crassa, germinating asexual spores (germlings) of ide

47 In N. crassa, iron transport is mediated by a number of sma

48 In N. crassa, polyamines repress the synthesis and increase

49 In N. crassa, SNZ and SNO homologs map to the region occupi

50 s must be identical at each het locus (11 in N. crassa) to form a stable heterokaryon.

51 cellulolytic gene expression and activity in N. crassa.

52 suggesting a minor role for this G alpha in N. crassa biology.

53 ts show that the powerful tools available in N. crassa allow for a comprehensive system level underst

54 ural timing of the robust circadian clock in N. crassa can be disrupted in the dark when maintained i

55 e hierarchy of initiation at start codons in N. crassa (AUG >> CUG > GUG > ACG > AUA approximately UU

56 that dynactin is not an essential complex in N. crassa.

57 tions to repress the onset of conidiation in N. crassa.

58 s during the vegetative and sexual cycles in N. crassa.

59 PA, is needed for entry of this defensin in N. crassa, but not in F. graminearum.

60 regulator of protoperithecial development in N. crassa, and double mutants carrying deletions of both

61 at arginase has a role in differentiation in N. crassa is being investigated.

62 ed point mutation (RIP) of repetitive DNA in N. crassa.

63 of temperature-regulated gene expression in N. crassa and suggest that the circadian feedback loop m

64 ucer of lignocellulolytic gene expression in N. crassa.

65 se data, we identified regulatory factors in N. crassa and characterized one (PDR-2) associated with

66 GNB-1, are essential for female fertility in N. crassa.

67 several Gbetagamma-independent functions in N. crassa.

68 ed and acts at the step of bilayer fusion in N. crassa.

69 However, deletion of the rca-1 gene in N. crassa had no major effect on growth rate, macroconid

70 erevisiae, inactivation of the pep-4 gene in N. crassa produced a phenotype that was different in sev

71 Deletion of the nik-1+ gene in N. crassa results in an organism that displays aberrant

72 -specific regulation on the reporter gene in N. crassa, but mutated or truncated uORFs did not, as de

73 e suggest that positively regulated genes in N. crassa are normally held in a transcriptionally repre

74 estion that not all clock-regulated genes in N. crassa are specifically involved in the development o

75 oximately 35% of genes marked by H3K27me3 in N. crassa are also H3K27me3-marked in Neurospora discret

76 polymorphic and fall into two haplogroups in N. crassa populations.

77 silencing of facultative heterochromatin in N. crassa, and perhaps in other organisms.

78 ivity is found to be significantly higher in N. crassa strains lacking uc-1, a putative regulatory ge

79 ar-cognate codons are used for initiation in N. crassa.

80 Thus, if rca-1 is involved in N. crassa development, its role is subtle or redundant.

81 Finally, expression of thymidine kinase in N. crassa enabled incorporation of bromodeoxyuridine int

82 s conidiation and adenylyl cyclase levels in N. crassa.

83 /NDT80 pathway is not involved in meiosis in N. crassa, but rather regulates the formation of female

84 ate the specific mark for DNA methylation in N. crassa.

85 ase that is essential for DNA methylation in N. crassa.

86 for HP1 localization and DNA methylation in N. crassa.

87 ntified, which may implicate mitochondria in N. crassa nonself recognition and PCD.

88 Internalization of MtDef4 in N. crassa is energy-dependent and involves endocytosis.

89 n the movement and distribution of nuclei in N. crassa hyphae remains unknown.

90 ming the existence of a second oscillator in N. crassa.

91 Most if not all paralogs in N. crassa duplicated and diverged before the emergence o

92 gulation by IME-2 of a cell death pathway in N. crassa that functions in concert with the VIB-1 cell

93 launching the pheromone response pathway in N. crassa.

94 Ectopic expression of phcA in N. crassa induced HI and cell death that was dependent o

95 um with six different haplogroups present in N. crassa populations.

96 says using the ACE reveal factors present in N. crassa protein extracts that recognize and bind speci

97 d sequences have been reported previously in N. crassa, we used methyl-binding-domain agarose chromat

98 s of beta-galactosidase activity produced in N. crassa strains expressing arg-2-lacZ fusion genes.

99 ence of MEI3, the only RAD51/DMC1 protein in N. crassa, demonstrating independence from the canonical

100 nerating functional variation of proteins in N. crassa, 3) there are different levels of evolutionary

101 epression, is similar to the role of RCO1 in N. crassa.

102 hlight the extent of circadian regulation in N. crassa and span transcriptional and translational pro

103 s our understanding of the light response in N. crassa, about which the most is known, and will then

104 on while maintaining light responsiveness in N. crassa when held in a steady metabolic state using bi

105 sting that NOP-1 functions as a rhodopsin in N. crassa photobiology.

106 e and meiotic silencing and RNA silencing in N. crassa.

107 og, an RDRP associated with RNA silencing in N. crassa.

108 ucleotide (nt) SSRs, the most common SSRs in N. crassa, was significantly biased in exons.

109 critical regulator of epigenome stability in N. crassa.

110 We show that osmotic stress in N. crassa induced the phosphorylation of a eukaryotic el

111 mutant that is used in circadian studies in N. crassa.

112 ble molecular markers for genetic studies in N. crassa.

113 no acid acquisition, 3.5-fold higher than in N. crassa.

114 . crassa mt LSU and ND1 introns with that in N. crassa mt tRNA(Tyr) by constructing three-dimensional

115 Here, we show that in N. crassa, two cellodextrin transporters, CDT-1 and CDT-

116 function of GNA-1 in signal transduction in N. crassa, we examined properties of strains with mutati

117 Nitrogen utilization in N. crassa is regulated by a network of pathway-specific

118 esistance to hygromycin when introduced into N. crassa.

119 h the most is known, and will then juxtapose N. crassa with A. nidulans, which, as will be described

120 the seven chromosomes comprising the 42.9-Mb N. crassa genome was resolved using two translocation st

121 s was identified from expression analysis of N. crassa grown on pure cellulose.

122 In mixed populations, cells of N. crassa and the phytopathogenic gray mold Botrytis cin

123 role in the spatial and temporal control of N. crassa and coordinates interactions that define clock

124 ed during the vegetative and sexual cycle of N. crassa in both A and a mating types.

125 Two forms of N. crassa DGAT2 were tested: the predicted full-length p

126 f the 5' and 3' regions of the spe-1 gene of N. crassa, required for this polyamine-mediated regulati

127 2749 SSRs of 963 SSR types in the genome of N. crassa.

128 nce or heat-/chemical-induced germination of N. crassa spores; however, this link is not consistent a

129 of crystalline precipitates on the hyphae of N. crassa showed that the main elements present in the c

130 comet-like forms for most of the wb mtDNA of N. crassa and Sc. pombe.

131 A vma-1 mutant of N. crassa largely metabolized methylammonium to methylgl

132 s identified in one of the double mutants of N. crassa conferred resistance to both bafilomycin and c

133 this study, pyridoxine-requiring mutants of N. crassa were found to possess mutations that disrupt c

134 in controlling the iron metabolic pathway of N. crassa.

135 Remarkably, the presence of N. crassa can reprogram this behavior and induce fusion

136 e, we performed transcriptional profiling of N. crassa on 40 different carbon sources, including plan

137 most eukaryotes, the centromeric regions of N. crassa are rich in sequences that are related to tran

138 nal changes in gating the photic response of N. crassa and indicate that LOV-LOV homo- or heterodimer

139 ed to this insertion in wild-type strains of N. crassa and other Neurospora species.

140 imaging of genetically engineered strains of N. crassa.

141 on, CdCl2 was contacted with supernatants of N. crassa obtained after growth in urea-containing mediu

142 reated a number of site-directed variants of N. crassa LAD that are capable of utilizing NADP(+) as c

143 the commonly used medium-copy-number pMOcosX N. crassa cosmid library in two independent screenings,

144 identified, including 10 of the 23 predicted N. crassa cellulases.

145 Comparisons with previous N. crassa CYT-18 structures and a structural model of th

146 ase analysis affirmed that the reconstituted N. crassa telomerase synthesizes TTAGGG repeats with hig

147 e distributions of the SSRs in the sequenced N. crassa genome differ systematically between chromosom

148 ndustrial scale enzymes in the model system, N. crassa, by removing the endogenous negative feedback

149 We found that N. crassa has a much higher proportion of "orphan" genes

150 We found that N. crassa rca-1 can complement the conidiation defect of

151 ssible, yet unvalidated, explanation is that N. crassa has an enhanced capacity for degrading charred

152 We show that N. crassa and F. graminearum respond differently to MtDe

153 We show that N. crassa cytoplasmic dynein and dynactin mutants have a

154 The results show that N. crassa uses a unique combination of polyamine-mediate

155 The N. crassa nrc-2 gene is the first member of this group o

156 There are remarkable differences between the N. crassa protein and its yeast homologue, including a r

157 MAPK [Osmotically Sensitive-2 (OS-2)] by the N. crassa circadian clock allows anticipation and prepar

158 ully the processes that are regulated by the N. crassa circadian clock, systematic screens were carri

159 The protein encoded by the N. crassa gene was longer than that of U. ramanniana.

160 hway in yeast, and we also characterized the N. crassa STE12 homolog pp-1.

161 The pep-4 gene appears to encode the N. crassa, homolog of proteinase A, but the maturation o

162 ate to reserve the pdx-1 designation for the N. crassa SNZ homolog and pdx-2 for the SNO homolog.

163 evidence of RIP; but one, isolated from the N. crassa host of Psi63, showed no evidence of RIP.

164 ins the canonical 5'-splice site GUAUGU, the N. crassa TER intron contains a non-canonical 5'-splice

165 , which account for 71% of total SSRs in the N. crassa genome, using a Poisson log-linear model.

166 genes would be expected to be present in the N. crassa genome.

167 we compared the CYT-18 binding sites in the N. crassa mt LSU and ND1 introns with that in N. crassa

168 Analyses of the 5'-leader regions in the N. crassa transcriptome revealed examples of highly cons

169 Two mutations in the N. crassa V-ATPase that affect the binding of bafilomyci

170 , and the new chondropsin class inhibits the N. crassa V-ATPase better than the chromaffin granule V-

171 ed that the minimal functional domain of the N. crassa AAP corresponded closely to the region that wa

172 Reconstitution of the N. crassa cellodextrin transport system in Saccharomyces

173 Predicted key features of the N. crassa clock system are a dynamically frustrated clos

174 Mutation or deletion of the N. crassa gene encoding subunit c' did not completely el

175 The analysis of the N. crassa genome sequence also reveals that RIP has impa

176 H3K27me3 covers 6.8% of the N. crassa genome, encompassing 223 domains, including 77

177 report a high-quality draft sequence of the N. crassa genome.

178 latively modest role in the evolution of the N. crassa genome.

179 Phylogenetic analysis of the N. crassa histone genes places them in the Euascomycota

180 We further evaluated the role of the N. crassa homolog of IME2, a kinase involved in initiati

181 ut surprisingly, at least in the case of the N. crassa mitochondrial (mt) large ribosomal subunit (LS

182 ding assays with deletion derivatives of the N. crassa mitochondrial large rRNA intron showed that at

183 sponding to the isolated P4-P6 domain of the N. crassa mitochondrial large subunit ribosomal RNA intr

184 The excellent fit of the N. crassa sequence to the E. hirae structure and the deg

185 ing methods refined our understanding of the N. crassa transcriptional response to cellulose and demo

186 ilable RNA and protein profiling data on the N. crassa clock.

187 e p24 proteins, whereas CBH-2 depends on the N. crassa homolog of yeast Erv29p.

188 totally defective in binding or splicing the N. crassa ND1 intron, but retains substantial residual a

189 Surprisingly, the N. crassa annexin homologue is most closely related to t

190 These data suggest that the N. crassa cellodextrin transporters act as "transceptors

191 We found that the N. crassa homologs of ISWI (NCU03875) and ACF1 (NCU00164

192 Thus, the N. crassa gene appears to be a functional homologue of A

193 RQ)/WCC feedback loop that is central to the N. crassa circadian system.

194 at the amino terminus that was unique to the N. crassa DGAT2 protein.

195 ster protein with greatest similarity to the N. crassa NIT4 protein that regulates genes required for

196 e interaction of the CYT-18 protein with the N. crassa mitochondrial large subunit ribosomal RNA (mt

197 plicing activity and TyrRS activity with the N. crassa mt tRNA(Tyr), but not for TyrRS activity with

198 vivo analysis of an F-box acting within the N. crassa sulfur regulatory network.

199 Thus, N. crassa germlings undergoing chemotropic interactions

200 g cDNAs revealed that ccg-12 is identical to N. crassa cmt encoding copper metallothionein, providing

201 suggesting a greater degree of similarity to N. crassa nit2 than to the areA-like genes that have bee

202 rches demonstrated that PAS is not unique to N. crassa PAS homologs likely influence the distribution

203 CHX that are associated with the translating N. crassa ribosome, spermidine is present near the CHX b

204 wide restriction site polymorphisms from two N. crassa strains: Mauriceville and Oak Ridge.

205 hich suppressed the sensitivity of wild type N. crassa to concanamycin, also proved effective in supp

206 The unique N. crassa TER 5'-splice site sequence is evolutionarily

207 her show that the P. syringae is able to use N. crassa as a sole nutrient source.

208 Using N. crassa expressing the Ca(2+) reporter aequorin, MsDef

209 enes that showed expression differences when N. crassa was cultured on ground Miscanthus stems as a s

210 However, the means by which N. crassa and other filamentous fungi sense the presence

211 Using a population of 110 wild N. crassa isolates, we investigated germling fusion betw

212 und that SDS3, RLP1, and RLP2 associate with N. crassa homologs of the Saccharomyces cerevisiae Rpd3L

213 pression data, the secretome associated with N. crassa growth on Miscanthus and cellulose was determi

214 te-associated DNA (RAD) mapping for use with N. crassa oligonucleotide microarrays.