ライフサイエンスコーパス: N. crassa

1 N. crassa H3K27me3-marked genes are less conserved than

2 N. crassa is considerably more morphologically and devel

3 N. crassa is of course a saprophyte and there is no comp

4 N. crassa is typically found on woody biomass and is com

5 ed the growth defect characteristic of dim-5 N. crassa but did not fully rescue the gross DNA hypomet

6 A N. crassa mutant carrying deletions for both transporter

7 A screen of a N. crassa transcription factor deletion collection ident

8 Previously, we have shown that a N. crassa mutant carrying deletions of three beta-glucos

9 In addition, N. crassa nitrite reductase displays several partial act

10 tDef4 against F. graminearum but not against N. crassa.

11 fourth component, Neurospora protein 55 (an N. crassa homolog of p55/RbAp48), is critical for H3K27m

12 hat the nonreducing end product formed by an N. crassa PMO is a 4-ketoaldose.

13 Here, we show that an N. crassa mutant carrying deletions of two genes encodin

14 of the AAP and the RNA encoding it using an N. crassa cell-free translation system.

15 We then analyzed N. crassa and Schizosaccharomyces pombe telomerase recon

16 H3K9 in both mouse embryonic stem cells and N. crassa.

17 ollowing the divergence of S. cerevisiae and N. crassa and provides insight into the evolution of kin

18 ) of mtDNA from Sc. pombe, S. cerevisiae and N. crassa, but bands without smears were found for diges

19 Both the A. niger and N. crassa proteins show homology with a stress-inducible

20 these chromosomes between N. tetrasperma and N. crassa.

21 he type exhibited by related species such as N. crassa.

22 This suggests that the widespread and basal N. crassa-type spliceosomal cleavage mechanism is more a

23 overall rate of molecular evolution between N. crassa and S. cerevisiae were not detected.

24 itation experiments, a heterocomplex between N. crassa HET-C1 and PhcA was associated with phcA-induc

25 d from a favored carbon source to cellulose, N. crassa dramatically up-regulates expression and secre

26 carbon source such as sucrose to cellulose, N. crassa dramatically upregulates expression and secret

27 Unlike S. cerevisiae, N. crassa has a single isoform of the a subunit.

28 ing translation extracts from S. cerevisiae, N. crassa, and wheat germ.

29 reated mutants of each of the four classical N. crassa HDAC genes and tested their effect on histone

30 was able to attach and extensively colonize N. crassa hyphae, while an Escherichia coli control show

31 We have developed N. crassa as a model system where we can dissect the com

32 size in linear form for all species (except N. crassa) and in multi-fibered, comet-like forms for mo

33 functional genomics resources available for N. crassa, which include a near-full genome deletion str

34 ne densities (12,000-13,000 total genes) for N. crassa.

35 A cell-free N. crassa system was developed that required the presenc

36 Analyses of purified QDE-1 polymerases from N. crassa (QDE-1(Ncr)) and related fungi, Thielavia terr

37 upon deletion of Puf4 in filamentous fungi (N. crassa) in contrast to the increase upon Puf3 deletio

38 inhibition (toeprint) assay in a homologous N. crassa cell-free translation system showed that argin

39 btained by translating mRNAs in a homologous N. crassa in vitro translation system or in rabbit retic

40 cosmids, on average, contained an identified N. crassa gene sequence as a starting point for gene ide

41 In N. crassa the mutant strains did not exhibit reduced spo

42 In N. crassa, germinating asexual spores (germlings) of ide

43 In N. crassa, iron transport is mediated by a number of sma

44 In N. crassa, polyamines repress the synthesis and increase

45 In N. crassa, SNZ and SNO homologs map to the region occupi

46 s must be identical at each het locus (11 in N. crassa) to form a stable heterokaryon.

47 cellulolytic gene expression and activity in N. crassa.

48 suggesting a minor role for this G alpha in N. crassa biology.

49 ts show that the powerful tools available in N. crassa allow for a comprehensive system level underst

50 ural timing of the robust circadian clock in N. crassa can be disrupted in the dark when maintained i

51 e hierarchy of initiation at start codons in N. crassa (AUG >> CUG > GUG > ACG > AUA approximately UU

52 that dynactin is not an essential complex in N. crassa.

53 tions to repress the onset of conidiation in N. crassa.

54 s during the vegetative and sexual cycles in N. crassa.

55 PA, is needed for entry of this defensin in N. crassa, but not in F. graminearum.

56 regulator of protoperithecial development in N. crassa, and double mutants carrying deletions of both

57 at arginase has a role in differentiation in N. crassa is being investigated.

58 ed point mutation (RIP) of repetitive DNA in N. crassa.

59 of temperature-regulated gene expression in N. crassa and suggest that the circadian feedback loop m

60 ucer of lignocellulolytic gene expression in N. crassa.

61 GNB-1, are essential for female fertility in N. crassa.

62 several Gbetagamma-independent functions in N. crassa.

63 ed and acts at the step of bilayer fusion in N. crassa.

64 However, deletion of the rca-1 gene in N. crassa had no major effect on growth rate, macroconid

65 erevisiae, inactivation of the pep-4 gene in N. crassa produced a phenotype that was different in sev

66 Deletion of the nik-1+ gene in N. crassa results in an organism that displays aberrant

67 -specific regulation on the reporter gene in N. crassa, but mutated or truncated uORFs did not, as de

68 e suggest that positively regulated genes in N. crassa are normally held in a transcriptionally repre

69 estion that not all clock-regulated genes in N. crassa are specifically involved in the development o

70 oximately 35% of genes marked by H3K27me3 in N. crassa are also H3K27me3-marked in Neurospora discret

71 ivity is found to be significantly higher in N. crassa strains lacking uc-1, a putative regulatory ge

72 ar-cognate codons are used for initiation in N. crassa.

73 Thus, if rca-1 is involved in N. crassa development, its role is subtle or redundant.

74 Finally, expression of thymidine kinase in N. crassa enabled incorporation of bromodeoxyuridine int

75 s conidiation and adenylyl cyclase levels in N. crassa.

76 /NDT80 pathway is not involved in meiosis in N. crassa, but rather regulates the formation of female

77 ate the specific mark for DNA methylation in N. crassa.

78 ase that is essential for DNA methylation in N. crassa.

79 for HP1 localization and DNA methylation in N. crassa.

80 ntified, which may implicate mitochondria in N. crassa nonself recognition and PCD.

81 Internalization of MtDef4 in N. crassa is energy-dependent and involves endocytosis.

82 n the movement and distribution of nuclei in N. crassa hyphae remains unknown.

83 ming the existence of a second oscillator in N. crassa.

84 Most if not all paralogs in N. crassa duplicated and diverged before the emergence o

85 gulation by IME-2 of a cell death pathway in N. crassa that functions in concert with the VIB-1 cell

86 launching the pheromone response pathway in N. crassa.

87 Ectopic expression of phcA in N. crassa induced HI and cell death that was dependent o

88 says using the ACE reveal factors present in N. crassa protein extracts that recognize and bind speci

89 d sequences have been reported previously in N. crassa, we used methyl-binding-domain agarose chromat

90 s of beta-galactosidase activity produced in N. crassa strains expressing arg-2-lacZ fusion genes.

91 ence of MEI3, the only RAD51/DMC1 protein in N. crassa, demonstrating independence from the canonical

92 nerating functional variation of proteins in N. crassa, 3) there are different levels of evolutionary

93 epression, is similar to the role of RCO1 in N. crassa.

94 s our understanding of the light response in N. crassa, about which the most is known, and will then

95 on while maintaining light responsiveness in N. crassa when held in a steady metabolic state using bi

96 sting that NOP-1 functions as a rhodopsin in N. crassa photobiology.

97 e and meiotic silencing and RNA silencing in N. crassa.

98 og, an RDRP associated with RNA silencing in N. crassa.

99 ucleotide (nt) SSRs, the most common SSRs in N. crassa, was significantly biased in exons.

100 We show that osmotic stress in N. crassa induced the phosphorylation of a eukaryotic el

101 mutant that is used in circadian studies in N. crassa.

102 ble molecular markers for genetic studies in N. crassa.

103 no acid acquisition, 3.5-fold higher than in N. crassa.

104 . crassa mt LSU and ND1 introns with that in N. crassa mt tRNA(Tyr) by constructing three-dimensional

105 Here, we show that in N. crassa, two cellodextrin transporters, CDT-1 and CDT-

106 function of GNA-1 in signal transduction in N. crassa, we examined properties of strains with mutati

107 esistance to hygromycin when introduced into N. crassa.

108 h the most is known, and will then juxtapose N. crassa with A. nidulans, which, as will be described

109 the seven chromosomes comprising the 42.9-Mb N. crassa genome was resolved using two translocation st

110 s was identified from expression analysis of N. crassa grown on pure cellulose.

111 ed during the vegetative and sexual cycle of N. crassa in both A and a mating types.

112 Two forms of N. crassa DGAT2 were tested: the predicted full-length p

113 f the 5' and 3' regions of the spe-1 gene of N. crassa, required for this polyamine-mediated regulati

114 2749 SSRs of 963 SSR types in the genome of N. crassa.

115 of crystalline precipitates on the hyphae of N. crassa showed that the main elements present in the c

116 comet-like forms for most of the wb mtDNA of N. crassa and Sc. pombe.

117 A vma-1 mutant of N. crassa largely metabolized methylammonium to methylgl

118 s identified in one of the double mutants of N. crassa conferred resistance to both bafilomycin and c

119 this study, pyridoxine-requiring mutants of N. crassa were found to possess mutations that disrupt c

120 in controlling the iron metabolic pathway of N. crassa.

121 most eukaryotes, the centromeric regions of N. crassa are rich in sequences that are related to tran

122 nal changes in gating the photic response of N. crassa and indicate that LOV-LOV homo- or heterodimer

123 ed to this insertion in wild-type strains of N. crassa and other Neurospora species.

124 imaging of genetically engineered strains of N. crassa.

125 on, CdCl2 was contacted with supernatants of N. crassa obtained after growth in urea-containing mediu

126 reated a number of site-directed variants of N. crassa LAD that are capable of utilizing NADP(+) as c

127 the commonly used medium-copy-number pMOcosX N. crassa cosmid library in two independent screenings,

128 identified, including 10 of the 23 predicted N. crassa cellulases.

129 Comparisons with previous N. crassa CYT-18 structures and a structural model of th

130 ase analysis affirmed that the reconstituted N. crassa telomerase synthesizes TTAGGG repeats with hig

131 e distributions of the SSRs in the sequenced N. crassa genome differ systematically between chromosom

132 ndustrial scale enzymes in the model system, N. crassa, by removing the endogenous negative feedback

133 We found that N. crassa has a much higher proportion of "orphan" genes

134 We found that N. crassa rca-1 can complement the conidiation defect of

135 We show that N. crassa and F. graminearum respond differently to MtDe

136 We show that N. crassa cytoplasmic dynein and dynactin mutants have a

137 The results show that N. crassa uses a unique combination of polyamine-mediate

138 The N. crassa nrc-2 gene is the first member of this group o

139 There are remarkable differences between the N. crassa protein and its yeast homologue, including a r

140 MAPK [Osmotically Sensitive-2 (OS-2)] by the N. crassa circadian clock allows anticipation and prepar

141 ully the processes that are regulated by the N. crassa circadian clock, systematic screens were carri

142 The protein encoded by the N. crassa gene was longer than that of U. ramanniana.

143 hway in yeast, and we also characterized the N. crassa STE12 homolog pp-1.

144 The pep-4 gene appears to encode the N. crassa, homolog of proteinase A, but the maturation o

145 ate to reserve the pdx-1 designation for the N. crassa SNZ homolog and pdx-2 for the SNO homolog.

146 evidence of RIP; but one, isolated from the N. crassa host of Psi63, showed no evidence of RIP.

147 ins the canonical 5'-splice site GUAUGU, the N. crassa TER intron contains a non-canonical 5'-splice

148 , which account for 71% of total SSRs in the N. crassa genome, using a Poisson log-linear model.

149 genes would be expected to be present in the N. crassa genome.

150 we compared the CYT-18 binding sites in the N. crassa mt LSU and ND1 introns with that in N. crassa

151 Analyses of the 5'-leader regions in the N. crassa transcriptome revealed examples of highly cons

152 Two mutations in the N. crassa V-ATPase that affect the binding of bafilomyci

153 , and the new chondropsin class inhibits the N. crassa V-ATPase better than the chromaffin granule V-

154 ed that the minimal functional domain of the N. crassa AAP corresponded closely to the region that wa

155 Reconstitution of the N. crassa cellodextrin transport system in Saccharomyces

156 Predicted key features of the N. crassa clock system are a dynamically frustrated clos

157 Mutation or deletion of the N. crassa gene encoding subunit c' did not completely el

158 The analysis of the N. crassa genome sequence also reveals that RIP has impa

159 H3K27me3 covers 6.8% of the N. crassa genome, encompassing 223 domains, including 77

160 report a high-quality draft sequence of the N. crassa genome.

161 latively modest role in the evolution of the N. crassa genome.

162 Phylogenetic analysis of the N. crassa histone genes places them in the Euascomycota

163 We further evaluated the role of the N. crassa homolog of IME2, a kinase involved in initiati

164 ut surprisingly, at least in the case of the N. crassa mitochondrial (mt) large ribosomal subunit (LS

165 ding assays with deletion derivatives of the N. crassa mitochondrial large rRNA intron showed that at

166 sponding to the isolated P4-P6 domain of the N. crassa mitochondrial large subunit ribosomal RNA intr

167 The excellent fit of the N. crassa sequence to the E. hirae structure and the deg

168 ing methods refined our understanding of the N. crassa transcriptional response to cellulose and demo

169 ilable RNA and protein profiling data on the N. crassa clock.

170 e p24 proteins, whereas CBH-2 depends on the N. crassa homolog of yeast Erv29p.

171 totally defective in binding or splicing the N. crassa ND1 intron, but retains substantial residual a

172 Surprisingly, the N. crassa annexin homologue is most closely related to t

173 These data suggest that the N. crassa cellodextrin transporters act as "transceptors

174 Thus, the N. crassa gene appears to be a functional homologue of A

175 RQ)/WCC feedback loop that is central to the N. crassa circadian system.

176 at the amino terminus that was unique to the N. crassa DGAT2 protein.

177 ster protein with greatest similarity to the N. crassa NIT4 protein that regulates genes required for

178 e interaction of the CYT-18 protein with the N. crassa mitochondrial large subunit ribosomal RNA (mt

179 plicing activity and TyrRS activity with the N. crassa mt tRNA(Tyr), but not for TyrRS activity with

180 vivo analysis of an F-box acting within the N. crassa sulfur regulatory network.

181 Thus, N. crassa germlings undergoing chemotropic interactions

182 g cDNAs revealed that ccg-12 is identical to N. crassa cmt encoding copper metallothionein, providing

183 suggesting a greater degree of similarity to N. crassa nit2 than to the areA-like genes that have bee

184 wide restriction site polymorphisms from two N. crassa strains: Mauriceville and Oak Ridge.

185 hich suppressed the sensitivity of wild type N. crassa to concanamycin, also proved effective in supp

186 The unique N. crassa TER 5'-splice site sequence is evolutionarily

187 her show that the P. syringae is able to use N. crassa as a sole nutrient source.

188 Using N. crassa expressing the Ca(2+) reporter aequorin, MsDef

189 enes that showed expression differences when N. crassa was cultured on ground Miscanthus stems as a s

190 However, the means by which N. crassa and other filamentous fungi sense the presence

191 However, the means by which N. crassa and other filamentous fungi sense the presence

192 Using a population of 110 wild N. crassa isolates, we investigated germling fusion betw

193 pression data, the secretome associated with N. crassa growth on Miscanthus and cellulose was determi

194 te-associated DNA (RAD) mapping for use with N. crassa oligonucleotide microarrays.