ライフサイエンスコーパス: NRSF

1 NRSF binds to the NRSE of the MOR gene in a sequence-spe

2 NRSF is normally down-regulated upon neuronal differenti

3 NRSF/REST has been demonstrated to regulate at least 30

4 NRSF/REST is a protein that silences transcription of a

5 he human transcription factors SRF, GABP and NRSF at an average resolution of about 20 base pairs.

6 ng transcription factor (REST, also known as NRSF) is a master repressor of neuronal gene expression

7 ilencing transcription factor; also known as NRSF) to its cognate RE1 sequences is temporally regulat

8 lencer of transcription (REST; also known as NRSF) transcription factor.

9 ng transcription factor (REST, also known as NRSF).

10 uggest that the synergic interaction between NRSF and Sp3 is required to negatively regulate MOR gene

11 was used to demonstrate interaction between NRSF/REST and REST4.

12 Blocking NRSF transiently after eFSE prevented granule cell dysma

13 Many of these putative NRSEs bound NRSF in vitro and repressed transcription in vivo.

14 elix is reversed compared to that adopted by NRSF, a transcription factor unrelated to SAP25, upon bi

15 repression of the cholinergic gene locus by NRSF/REST.

16 Down-regulation of NRP1 by NRSF overexpression reduced Sema3A activity.

17 is dsRNA is NRSE/RE1, which is recognized by NRSF/REST, known primarily as a negative transcriptional

18 Genes selectively regulated by NRSF during epileptogenesis coded for ion channels, rece

19 Unexpectedly, genes selectively repressed by NRSF had mid-range binding frequencies to the repressor,

20 isms involving transcriptional repression by NRSF.

21 cells with neuron-specific genes silenced by NRSF/REST into cells with neuronal identity that can exp

22 The DNA-binding protein REST (also called NRSF) is a transcriptional repressor that targets many n

23 -silencing transcription factor; also called NRSF) is expressed at high levels in mouse embryonic ste

24 posite NRSE to identify additional candidate NRSF target genes.

25 his cis-activating NRSE element also confers NRSF-dependent modulation in the context of the native p

26 a DNA-protein complex with NRSF, and confers NRSF-dependent transcriptional repression in the context

27 Although nuclear extracts containing NRSF/REST from A126.1B2 exhibited binding to NRSE/RE-1,

28 814, 5813 and 73 956 binding sites for CTCF, NRSF and STAT1 proteins, respectively, which is 32, 299

29 targeted mutation of Rest, the gene encoding NRSF, caused derepression of neuron-specific tubulin in

30 In the co-immunoprecipitation experiment, NRSF interacted with the full-length Sp3 factor, but not

31 fferentiated while constitutively expressing NRSF showed a significantly increased frequency of axon

32 The neuron-restrictive silencer factor NRSF (also known as REST and XBR) can silence transcript

33 sion is mediated by the transcription factor NRSF, which recruits the NADH-binding co-repressor CtBP

34 d ChIP-seq data for the transcription factor NRSF/REST, a study of ChIP-seq analysis with or without

35 ending on neuron-restriction silence factor (NRSF) expression levels.

36 that the neuron restrictive silencer factor (NRSF) binds to NRSE.

37 r (REST)/neuron-restrictive silencer factor (NRSF) can repress several terminal neuronal differentiat

38 r (REST)/neuron-restrictive silencer factor (NRSF) can repress transcription of a battery of neuronal

39 et al.'s neuron-restrictive silencer factor (NRSF) ChIP-Seq data without relying on extensive qPCR va

40 r (REST)/neuron-restrictive silencer factor (NRSF) plays a critical role in elaboration of the neuron

41 The neuron-restrictive silencer factor (NRSF) represses transcription of several neuronal genes

42 ulators, neuron-restrictive silencer factor (NRSF), has been shown to repress the expression of neuro

43 epressor neuron-restrictive silencer factor (NRSF), which negatively regulates Crh gene transcription

44 Neuron-restrictive silencer factor (NRSF)/RE-1-silencing transcription factor (REST), the tr

45 n of the neuron-restrictive silencer factor (NRSF/REST), an important transcription factor that influ

46 The neuron-restrictive silencer factor (NRSF; also known as REST for repressor element-1 silenci

47 g of the neuron-restrictive silencer factor (NRSF; also known as REST, for repressor element-1 silenc

48 pressor neuron restrictive silencing factor (NRSF or REST).

49 factor/neuron restrictive silencing factor (NRSF).

50 ent-1 (RE-1) silencing transcription factor (NRSF/REST) contains nine zinc finger domains and binds t

51 The neuron-restrictive silencer factor [NRSF (RE-1 silencing transcription factor/X box represso

52 iption factors: CTCF (CCCTC-binding factor), NRSF (neuron-restrictive silencer factor) and STAT1 (sig

53 or element-1 silencing transcription factor)/NRSF (neuron-restrictive silencing factor) and REST-depe

54 known as neuron-restrictive silencer factor, NRSF) is a universal feature of normal ageing in human c

55 known as neuron restrictive silencer factor, NRSF).

56 known as neuron restrictive silencer factor, NRSF).

57 or element 1 silencing transcription factor]/NRSF (neuron-restrictive silencer factor) actively repre

58 ChIP-seq data for the transcription factors NRSF and GABP.

59 -factor genes, and these tested positive for NRSF/REST occupancy in vivo.

60 ession of the pax4 gene and infer a role for NRSF in pancreatic islet development.

61 ngs suggest an important functional role for NRSF in the expression of the pax4 gene and infer a role

62 Acquired HCN1 channelopathy derives from NRSF-mediated transcriptional repression that endures vi

63 he NRSE of the MOR promoter is functional in NRSF-positive cells (NS20Y and HeLa) but not in NRSF-neg

64 F-positive cells (NS20Y and HeLa) but not in NRSF-negative cells (PC12).

65 Increased NRSF binding to chromatin was accompanied by sequential

66 g neurons dissipated by adulthood, increased NRSF levels and repression of CRH expression persisted,

67 nvertebrate and protochordate genomes, as is NRSF itself.

68 pilepsy-provoking seizures increased the low NRSF levels in mature hippocampus several fold yet surpr

69 ng the transcription factor REST (also named NRSF or XBR).

70 RNA and protein, whereas a dominant negative NRSF increased NRP1.

71 either trichostatin A or a dominant-negative NRSF induced MOR promoter activity and transcription of

72 tifs and allowed us to identify noncanonical NRSF-binding motifs.

73 hromatin immunoprecipitation-seq analyses of NRSF targets identified gene networks that, in addition

74 REST4 inhibited binding of NRSF/REST to NRSE/RE-1 as determined by gel mobility shi

75 Constitutive expression of NRSF in the developing spinal cord of chicken embryos ca

76 d them sensitive to moderate fluctuations of NRSF levels.

77 n embryos, using a dominant-negative form of NRSF, also caused derepression of neuronal tubulin, as w

78 REST4, a C-terminally truncated form of NRSF/REST, contains the five N-terminal zinc fingers and

79 Mosaic inhibition of NRSF in chicken embryos, using a dominant-negative form

80 se inhibitor trichostatin A, an inhibitor of NRSF silencing activity, also increased NRP1 levels.

81 Levels of NRSF and its physical binding to the Hcn1 gene were augm

82 Overexpression of NRSF in HaCaT cells decreased NRP1 RNA and protein, wher

83 PCR validated NRSF sites and the presence of NRSF binding motifs for setting thresholds.

84 These data suggest that down-regulation of NRSF is necessary for the proper development of at least

85 Thus, dynamic, selective regulation of NRSF target genes may play a role in influencing neurona

86 ression, concomitant with down-regulation of NRSF.

87 This provides direct evidence the role of NRSF in the cells and also indicates that NRSF expressio

88 The suppression of NRSF activity with either trichostatin A or a dominant-n

89 d downregulation of Tbx3 and upregulation of NRSF and miR-1 (transcriptional regulators) that explain

90 sion in specific neuronal cells depending on NRSF expression level.

91 llers, including candidate feedback loops on NRSF and its corepressor, CoREST.

92 epressive epigenetic changes which outlasted NRSF binding.

93 y a subset ( approximately 10%) of potential NRSF target genes.

94 ement [NRSE]), in vitro and in vivo, reduced NRSF binding to Hcn1, prevented its repression, and rest

95 cally tested site for the neuronal repressor NRSF/REST, Cistematic generated a refined PSFM (position

96 Physical interaction of the repressor, NRSF, was abolished using decoy oligodeoxynucleotides (O

97 hat bind the transcriptional repressor REST (NRSF) encode in vivo DNA binding affinity hierarchies th

98 r RE-1-silencing transcription factor (REST)/NRSF, both the NRSE and sequences in the first intron we

99 REST/NRSF (repressor-element-1-silencing transcription factor

100 REST/NRSF acts as a regulator of neuron-specific gene express

101 REST/NRSF is a transcriptional repressor that acts at the ter

102 REST/NRSF is expressed most highly in non-neural tissues, whe

103 REST/NRSF is generally downregulated during induction of neur

104 REST/NRSF recruits CoREST and mSin3A corepressors to stem cel

105 REST/NRSF was first identified as a transcriptional repressor

106 To determine whether c-Myc and REST/NRSF act together to cause medulloblastomas, we used a p

107 sential link between H3K4 complexes and REST/NRSF and provide the first direct genetic evidence that

108 hese experiments show that the NRSE and REST/NRSF are important components in restricting L1 expressi

109 t fashion in both muscle-derived L6 and REST/NRSF co-transfected neuronal PC12 cells.

110 of neural progenitor proliferation, and REST/NRSF, a transcriptional repressor of neuronal differenti

111 nscription and recruits RBPJ/Sin3A- and REST/NRSF-repressive complexes to repress p14(ARF) and p16(IN

112 g the nuclear localization of REST4 and REST/NRSF.

113 ereas Esco2 is infrequently enriched at REST/NRSF target genes.

114 te system showed an interaction between REST/NRSF and RILP as well as between RILP and dynactin p150(

115 ferase constructs was down-regulated by REST/NRSF in a RE-1/NRSE-dependent fashion in both muscle-der

116 sing hormone (CRH) gene is regulated by REST/NRSF, in part through the RE-1/NRSE.

117 long-term repression events mediated by REST/NRSF.

118 malian cell types and activate cellular REST/NRSF target genes, even in the absence of factors that a

119 al units that do not themselves contain REST/NRSF response elements.

120 into the nucleus and that HAP1 controls REST/NRSF cellular localization in neurons.

121 dulloblastoma cells was able to counter REST/NRSF-mediated repression of neuronal promoters, stimulat

122 In addition, countering REST/NRSF function blocked the tumorigenic potential of NSC-M

123 ds to the same DNA binding site as does REST/NRSF but functions as an activator instead of a represso

124 n did not interact directly with either REST/NRSF or RILP, but did interact with dynactin p150(Glued)

125 was able to compete with the endogenous REST/NRSF for DNA binding and stimulate neuronal promoters.

126 entiation pathway, countered endogenous REST/NRSF-dependent repression, activated the REST/NRSF targe

127 tor/neuron-restrictive silencer factor (REST/NRSF) at very high levels compared with either neuronal

128 r/neuronal restricted silencing factor (REST/NRSF) can mediate extraneuronal restriction by imposing

129 tor/neuron-restrictive silencer factor (REST/NRSF) complex.

130 or/neuron-restrictive silencing factor (REST/NRSF) in many neuron-specific genes.

131 tor/Neuron-Restrictive Silencer Factor (REST/NRSF) is a gene-silencing factor that is widely expresse

132 tor/neuron-restrictive silencer factor (REST/NRSF) silences neuronal genes in neural stem cells (NSCs

133 or/neuron-restrictive silencing factor (REST/NRSF), a known tumor suppressor, transcriptionally repre

134 ion/neuron-restrictive silencer factor (REST/NRSF)--thought to regulate hundreds of neuron-specific g

135 the RE1-silencing transcription factor (REST/NRSF).

136 tor/neuron-restrictive silencer factor (REST/NRSF).

137 evealed seemingly paradoxical roles for REST/NRSF in neurogenesis, neural plasticity, tumour suppress

138 ion in PRICKLE1 (also known as RILP for REST/NRSF interacting LIM domain protein) in all three of the

139 unrecognized tumor suppressor role for REST/NRSF, a transcriptional repressor of neuronal gene expre

140 aller set of genes, including those for REST/NRSF, Groucho, nucleophosmin, and Ubc4/5E2.

141 ears to serve as a nuclear receptor for REST/NRSF, REST4, and possibly other transcription factors.

142 ndent genes contained binding sites for REST/NRSF, suggesting that release from general repression in

143 We propose the name RILP, for REST/NRSF-interacting LIM domain protein, to label this novel

144 However, REST/NRSF(-/-) mice suggest that the absence of REST/NRSF-dep

145 Our work identifies REST/NRSF as a master negative regulator of adult NSC differe

146 Moreover, mice lacking REST/NRSF specifically in NSCs display a transient increase i

147 In another neuronal cell line, NG108, REST/NRSF also repressed expression from constructs containin

148 udies have shown that neither c-Myc nor REST/NRSF alone could cause tumor formation.

149 gs indicate that abnormal expression of REST/NRSF and Myc in NSCs causes cerebellum-specific tumors b

150 e shown that heterologous expression of REST/NRSF in Saccharomyces cerevisiae is able to repress tran

151 omplex involved in the translocation of REST/NRSF into the nucleus and that HAP1 controls REST/NRSF c

152 1/NRSE-mediated repressive influence of REST/NRSF is well established, results in transgenic studies

153 esults showed that direct activation of REST/NRSF target genes in NSCs with a single transgene, REST-

154 sults suggest that direct activation of REST/NRSF target genes with a single transgene, REST-VP16, is

155 directly activate the transcription of REST/NRSF target genes.

156 VP16, by replacing repressor domains of REST/NRSF with the activation domain of a viral activator VP1

157 ucted by replacing repressor domains of REST/NRSF with the activation domain of viral protein (VP16).

158 To counter the effect of REST/NRSF, we used a recombinant transcription factor, REST-V

159 F(-/-) mice suggest that the absence of REST/NRSF-dependent repression alone is not sufficient for th

160 the N-terminal Sin3p binding domain of REST/NRSF.

161 cells mediated by the repressor protein REST/NRSF (RE1 silencing transcription factor/neural-restrict

162 Its cognate binding protein, REST/NRSF, is an essential transcription factor; its null mut

163 (RILP) has also been shown to regulate REST/NRSF nuclear translocation.

164 ered to express a doxycycline-regulated REST/NRSF transgene (NSC-M-R), they no longer underwent termi

165 ethylation complex, directly regulating REST/NRSF, a master regulator of neural gene expression and c

166 8 may associate with another regulator, REST/NRSF, predominately at promoter regions via studying sev

167 The transcriptional repressor REST/NRSF (RE-1 silencing transcription factor/neuron-restric

168 tudies of the transcriptional repressor REST/NRSF (RE1 Silencing Transcription Factor or Neural Restr

169 The neuronal gene repressor REST/NRSF recruits corepressors, including CoREST, to modify

170 The repressor REST/NRSF restricts expression of a large set of genes to neu

171 We show that REST/NRSF binding to RE1/NRSE is accompanied by a decrease in

172 Furthermore, we show that REST/NRSF binds mammalian SIN3A and HDAC-2 and requires histo

173 These results suggest that REST/NRSF can act as both a repressor of Crh transcription, v

174 Here we report that REST/NRSF is required to maintain the adult neural stem cell

175 res both yeast Sin3p and Rpd3p and that REST/NRSF physically interacts with the product of the yeast

176 We show that REST/NRSF recruits SCPs to neuronal genes that contain RE-1 e

177 Taken together, these data suggest that REST/NRSF represses neuronal gene transcription by recruiting

178 cells with trichostatin A revealed that REST/NRSF repression depends, in part, on histone deacetylase

179 Thus, this study suggests that REST/NRSF, dynactin p150(Glued), huntingtin, HAP1, and RILP f

180 er activity requires the binding of the REST/NRSF repressor complex in nonneuronal cells.

181 RSF-dependent repression, activated the REST/NRSF target genes, and, surprisingly, activated other ne

182 The REST/NRSF-interacting LIM domain protein (RILP) has also been

183 Therefore, REST/NRSF may serve as a new target for therapeutic intervent

184 the same RE1/NRSE, but activates these REST/NRSF target genes.

185 revents this complex from translocating REST/NRSF to the nucleus.

186 However, unexpectedly, REST/NRSF up-regulated expression levels of constructs lackin

187 se-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P1

188 ecruits the histone methylase G9a to silence NRSF target genes in nonneuronal cells.

189 Some NRSF/REST sites reside in repeats, which suggests a mech

190 scription-associated factors, including SRF, NRSF, GABP, Stat3 and p300 in different developmental co

191 g of the repressor protein REST (also termed NRSF or XBR) to the RE1 (also called NRSE) sequence in t

192 Analysis of two well-studied TFs, NRSF and CCCTC-binding factor (CTCF), also suggests that

193 It was concluded that NRSF is a transcription factor that silences NRP1 expres

194 in several nonneuronal genes, implying that NRSF may play a broader role than originally anticipated

195 These results indicate that NRSF is required to repress neuronal gene expression in

196 of NRSF in the cells and also indicates that NRSF expression is regulated by post-translational modif

197 Here we report that NRSF recruits the histone methylase G9a to silence NRSF

198 Our data suggested that NRSF can function as a repressor of MOR transcription in

199 factor genes contain NRSEs, suggesting that NRSF may repress neuronal differentiation both directly

200 The NRSF splice variant represents a specific clinical marke

201 The NRSF/REST isoform REST4 was expressed in PC12 cells but

202 Administration of decoy ODNs comprising the NRSF DNA-binding sequence (neuron restrictive silencer e

203 ent with NRSE in NS20Y cells, but not in the NRSF negative PC12 cells.

204 re we describe a novel splice variant of the NRSF transcript, which is highly expressed in SCLCs.

205 When the NRSF was disrupted in NS20Y and HeLa cells using small i

206 we have identified core residues within the NRSF and CTCF binding sites that are critical for a stro

207 Thus, up-regulated expression of this NRSF isoform may be a key early factor in defining the n

208 on and predicting translation of a truncated NRSF isoform.

209 without relying on extensive qPCR validated NRSF sites and the presence of NRSF binding motifs for s

210 gly, the repressed gene-set was rescued when NRSF binding to chromatin was blocked.

211 evolution, forms a DNA-protein complex with NRSF, and confers NRSF-dependent transcriptional repress

212 uron-specific genes through interaction with NRSF/REST transcriptional machinery, resulting in the tr