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1 ity within the hepatitis C virus polymerase (NS5B).
2 n of the viral RNA-dependent RNA polymerase (NS5B).
3 osine as a potent and selective inhibitor of NS5B.
4 arily conferred by the S282T substitution in NS5B.
5 s is the viral RNA-dependent RNA polymerase, NS5B.
6 patitis C virus (HCV) proteins NS3, NS4, and NS5B.
7 information on QAZ binding interactions with NS5B.
8 rotease and the RNA-dependent RNA polymerase NS5B.
9 ophobic binding pocket at the palm region of NS5B.
10 ted, HCV-796 showed slow binding kinetics to NS5B.
11 include a 1:1 complex formed between NS3 and NS5B.
12 n the helicase, NS3, and the RNA polymerase, NS5B.
13 oduct formation increased in the presence of NS5B.
14 SNPs and rare and common RAVs within NS3 and NS5B.
15 tivity, which is specifically antagonized by NS5B.
16 es 2629 to 2637 of nonstructural protein 5B (NS5B(2629-2637)) (KSKKTPMGF) and E2(541-549) (NTRPPLGNW)
17 the subdominant HLA-B27-restricted epitope, NS5B(2936-2944) (GRAAICGKY), to further define the mecha
18 -NS5A (5-5A) from genotypes 1-6 and 2a(JFH1) NS5B-3' untranslated region, and tested the effects of N
19 5' untranslated region (5'UTR)-NS5A and JFH1 NS5B-3'UTR; recovered viruses acquired two adaptive muta
21 hat the culture-adaptive mutations in p7 and NS5B acted synergistically to enhance the specific viral
22 ns thus add to the diversifying pool of anti-NS5B agents and provide a novel scaffold for structural
23 mergent substitutions occurring at conserved NS5B amino acid positions in subjects who experienced vi
29 prehensive description of RNA replication by NS5B and is relevant to understanding the functional pro
30 e for the viral RNA-dependent RNA polymerase NS5B and p7 proteins in contributing to virion morphogen
31 study the effects of inhibitors of NS5A and NS5B and resistance to sofosbuvir-the only nucleotide an
32 ably low dissociation rate for wild type HCV NS5B, and a highly dynamic enzyme-RNA binary complex.
33 ts of baseline hepatitis C virus (HCV) NS5A, NS5B, and NS3 resistance-associated substitutions (RASs)
36 between the Delta1 loop and thumb domain in NS5B are required for de novo initiation, although it is
37 reveal a genetic interaction between p7 and NS5B, as well as an interaction with sphingomyelin that
42 his loss of replication competence, purified NS5B-C316A protein was two- to threefold more active tha
43 associated variants were identified, and the NS5B changes observed did not confer significant reducti
45 ssociated with mutational escape of NS5a and NS5b class I epitopes that had also mutated during the f
46 cting replication element located within the NS5B coding region of hepatitis C virus (HCV) were deter
47 netically conserved RNA structure within the NS5B coding region of hepatitis C virus functions as a c
51 with a strain-DBN coding sequence, modified NS5B consensus sequence, pS52 untranslated regions, and
52 ne SA13/JFH1orig mutant termed SA13/JFH1Core-NS5B, containing 13 amino acid changes (R114W and V187A
53 that under optimized conditions up to 65% of NS5B could be converted into active elongation complexes
55 Despite this importance and the existence of NS5B crystal structures, our understanding of the confor
56 he NS3-helicase (F1464L), NS4A (A1672S), and NS5B (D2979G) were adapted and improved, by incorporatin
59 data highlight the potential of using NMR of NS5B(Delta21) as a powerful tool to characterize in solu
60 gh quality heteronuclear NMR spectrum of HCV NS5B(Delta21) has been obtained and was used to characte
61 cyclosporine A added to a sample containing NS5B(Delta21), NS5A-D2, and CypA specifically inhibits t
65 2266P, and I2340T [NS5A]; A2500S and V2841A [NS5B]), displayed fitness comparable to that of the poly
66 virals targeting the HCV protease, NS5A, and NS5B, each inhibited full-length TN infection dose-depen
67 9) as a potent inhibitor of RNA synthesis by NS5B (EC(50) = 1.28 microM), the RNA polymerase encoded
68 d 56, which had an excellent potency in both NS5B enzyme (IC(50) = 0.008 muM) and cell-based replicon
74 ial natural drug-resistance mutations in the NS5B gene of HCV genotype 1b from treatment-naive patien
75 is associated with distinct mutations in the NS5B gene that increase RNA binding in the presence of C
76 -based sequencing and 454 deep sequencing of NS5B gene were performed on plasma and liver samples obt
78 sequence analyses of the HCV NS3, NS5A, and NS5B genes were performed on blood samples collected at
82 ucleoside inhibitors (NI) of HCV polymerase (NS5B) have demonstrated pan-genotypic activity and durab
88 f the conformational and dynamic behavior of NS5B in solution and its relationship with NS5A-D2 remai
90 of HCV RNA-dependent RNA polymerase (RdRp), NS5B, in mouse liver and human hepatocytes results in pr
91 n of an escape variant with substitutions in NS5B, including the resistance-associated substitution S
93 itory beta-hairpin loop from genotype 2a HCV NS5B increases de novo RNA synthesis by >100-fold, promo
94 to the discovery of the first thumb pocket 1 NS5B inhibitor (BILB 1941) that demonstrated antiviral a
95 atitis C virus (HCV) NS5A inhibitor, and the NS5B inhibitor sofosbuvir has shown efficacy in patients
96 inhibitor vedroprevir (VDV), non-nucleoside NS5B inhibitor tegobuvir (TGV), and ribavirin (RBV) in t
98 (BI 207524, 27) to the first thumb pocket 1 NS5B inhibitor to demonstrate antiviral activity in geno
99 groups, leading to the first thumb pocket 2 NS5B inhibitor with picomolar antiviral potency in genot
100 nation with sofosbuvir at 400 mg once daily (NS5B inhibitor) and ribavirin at 600 mg/day for 12 weeks
101 rs should be retreated with sofosbuvir (SOF; NS5B inhibitor) combined with simeprevir (SIM; protease
102 450 mg once daily) of uprifosbuvir (MK-3682; NS5B inhibitor) in an 8-week regimen combined with grazo
103 nhibitor) plus sofosbuvir (SOF; pangenotypic NS5B inhibitor) in patients infected with genotype 3.
104 inhibitor), and BMS-791325 (a non-nucleoside NS5B inhibitor), in patients with chronic HCV infection.
105 se inhibitor), and beclabuvir (nonnucleoside NS5B inhibitor), with or without ribavirin, was evaluate
107 e to characterize mechanism of inhibition of NS5B inhibitors against either template RNA poly(A) or p
109 ort the identification of a new class of HCV NS5B inhibitors belonging to the coumestan family of phy
117 nt specifically blocked the incorporation of NS5B into the RC without affecting either the total prot
119 teraction with the induced binding pocket on NS5B is consistent with slow binding kinetics and loss o
121 ase from the Hepatitis C Virus (gene product NS5B) is a validated drug target because of its critical
122 tis C virus (HCV) non-structural protein 5B (NS5B) is an RNA-dependent RNA polymerase that is essenti
125 ct monitoring of the interaction between HCV NS5B, its RNA template, and finger loop inhibitors.
126 y escape mutations within the immunodominant NS5B KSKKTPMGF epitope may contribute in part to the con
127 c T lymphocyte (CTL) escape mutations in the NS5B KSKKTPMGF epitope might impair viral replication an
130 his compound against the clinically relevant NS5B M423T mutant, relative to the wild type, was shown
133 ear whether these interactions are within an NS5B monomer or are part of a higher-order NS5B oligomer
135 h the NS5A inhibitor ledipasvir (LDV) or the NS5B non-nucleoside inhibitor GS-9669 in patients with g
136 ibitor with ritonavir (r) (ABT-450/r) and an NS5B non-nucleoside polymerase inhibitor (ABT-333), with
137 n with genetic vaccines encoding the HCV NS3-NS5b nonstructural proteins during DAA treatment resulte
138 hepatitis C viral genome is catalyzed by the NS5B (nonstructural protein 5B) RNA-dependent RNA polyme
140 rehension of the molecular interplay between NS5B, NS5A, and CypA, three essentials proteins for HCV
141 quent high-resolution mutational analysis of NS5B (nt 7787 to 9289) using approximately 51-nucleotide
142 next-generation NS3 protease inhibitors, and NS5B nucleoside and nonnucleoside inhibitors, has provid
146 regimen of the NS5A inhibitor ledipasvir and NS5B nucleotide polymerase inhibitor sofosbuvir was show
147 is C virus NS5A inhibitor ledipasvir and the NS5B nucleotide polymerase inhibitor sofosbuvir with and
148 mographic data was created by sequencing the NS5B of 125 genotype 1a (Gt1a) samples and 166 Gt3a samp
150 presence of DAA-resistant variants in NS5A, NS5B, or NS3 (including telaprevir-resistant variants),
151 nd structures of three structurally distinct NS5B palm site allosteric inhibitors, the high-throughpu
152 or a representative derivative was mapped to NS5B palm site I employing a mutant counterscreen assay,
156 d inhibition of: HCV cell culture infection, NS5B polymerase activity, TNF-alpha-induced NF-kappaB tr
158 series, exhibiting a IC50 of 7.9 muM against NS5B polymerase and antiviral effect (EC50 = 8.1 muM; EC
159 leosides are selective inhibitors of the HCV NS5B polymerase and have demonstrated potent activity in
160 xhibiting an IC50 value of 0.069 muM against NS5B polymerase and selective antiviral effect (EC50 = 3
163 longation complex of the medically important NS5B polymerase for structural and functional studies.
165 Starting from indole-based C-3 pyridone HCV NS5B polymerase inhibitor 2, structure-activity relation
166 BILN2061, ITMN-191, SCH6 and Boceprevir; the NS5B polymerase inhibitor AG-021541; and to the NS4A ant
167 7), marketed as Daklinza, and the allosteric NS5B polymerase inhibitor beclabuvir (142), which is in
168 bitor ABT-450 (ABT-450/r), the nonnucleoside NS5B polymerase inhibitor dasabuvir, and ribavirin in li
170 Resistance to mericitabine (prodrug of HCV NS5B polymerase inhibitor PSI-6130) is rare and conferre
171 We assessed the efficacy and safety of the NS5B polymerase inhibitor sofosbuvir and the NS5A inhibi
172 y with the NS5A inhibitor ledipasvir and the NS5B polymerase inhibitor sofosbuvir in patients with HC
173 bitor) + sofosbuvir (HCV nucleotide analogue NS5B polymerase inhibitor) +/- ribavirin for 12 or 24 we
174 ) plus sofosbuvir (a nucleotide analogue HCV NS5B polymerase inhibitor) in patients infected with HCV
175 /r), in addition to ABT-333, a nonnucleoside NS5B polymerase inhibitor, and ribavirin, for the treatm
176 5A inhibitor, ledipasvir, combined with the NS5B polymerase inhibitor, sofosbuvir, in patients with
178 hange conferring resistance to nucleos(t)ide NS5B polymerase inhibitors and in 16 patients the C316N/
179 stance to SOF and other nucleos(t)ide analog NS5B polymerase inhibitors are not well understood.
180 acid series of allosteric thumb pocket 2 HCV NS5B polymerase inhibitors exhibited hindered rotation a
181 ants resistant to nucleo(s/t)ide active-site NS5B polymerase inhibitors were not observed, whereas va
182 se inhibitor class and other classes such as NS5B polymerase inhibitors, NS5A inhibitors and cyclophi
188 V polymerase NS5B to wild-type and resistant NS5B polymerase proteins was determined, and the influen
189 omer of a compound bound to the intended HCV NS5B polymerase target whereas the mirror image atropiso
190 iophene carboxylate allosteric inhibitors of NS5B polymerase that act at the thumb pocket 2 site.
191 b pocket 1) non-nucleoside inhibitors of HCV NS5B polymerase that inhibit replication in replicon sys
192 triphosphate form specifically inhibited HCV NS5B polymerase with no marked inhibition of human polym
193 ic template with potent activity against the NS5B polymerase, a critical enzyme on the life cycle of
194 ng chemotype against hepatitis C virus (HCV) NS5B polymerase, a validated and promising anti-HCV targ
195 uinolone derivatives as potent inhibitors of NS5B polymerase, an attractive and viable therapeutic ta
196 hepatitis C viral proteins NS3/4A protease, NS5B polymerase, and NS5A are clinically validated targe
198 conformation of the NS3-4A protease and the NS5B polymerase, membrane-bound HCV replicase components
200 rated that HCV NS3/NS4A protease-, NS5A- and NS5B polymerase-directed drugs respectively inhibited fu
210 de of work, the formation of a highly active NS5B polymerase.RNA complex suitable for mechanistic and
213 C virus (HCV) requires proteins from the NS3-NS5B polyprotein to create a replicase unit for replicat
214 hat highly conserved active-site residues in NS5B position the primer for in-line attack on the incom
215 tions, we were able to generate a productive NS5B.primer.template elongation complex stalled after fo
217 In this study, we evaluate the impact of HCV-NS5B prodrug phosphoramidate diastereochemistry (D-/L-al
218 ythmia following coadministration of the HCV-NS5B prodrug sofosbuvir with amiodarone was recently rep
219 periments demonstrated that only certain HCV-NS5B prodrugs elicit bradycardia when combined with amio
220 er experiments revealed that the HCV NS2 and NS5B proteins are responsible for the HCV-associated dec
222 Here, we demonstrate that the viral p7 and NS5B proteins cooperate to promote virion infectivity by
224 of reduction of NNI binding affinity for the NS5B proteins with various resistance mutations in the p
225 es revealed the localization of HCV NS5A and NS5B proteins, which are two important components of the
234 The RNA-dependent RNA polymerase enzyme NS5B represents one such DAA therapeutic target for inhi
235 gue inhibitor of the hepatitis C virus (HCV) NS5B RNA polymerase that is efficacious in subjects with
237 arin but not silibinin inhibited genotype 2a NS5B RNA-dependent RNA polymerase (RdRp) activity at con
238 C viral (HCV) genome is accomplished by the NS5B RNA-dependent RNA polymerase (RdRp), for which mech
241 as an alternative substrate inhibitor of the NS5B RNA-dependent RNA polymerase during HCV replication
242 ne ribonucleosides form a novel class of HCV NS5B RNA-dependent RNA polymerase inhibitors, displaying
243 hese include new NS3/4A protease inhibitors, NS5B RNA-dependent RNA polymerase inhibitors, NS5A inhib
246 ations, identified in the p7 polypeptide and NS5B RNA-dependent RNA polymerase, were sufficient to in
247 log inhibitor of the hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase, with activity across
248 chanism for formation of a productive binary NS5B-RNA complex, here NS5B slides along the RNA templat
249 dies revealed important properties of static NS5B-RNA complexes, the nature and relevance of dynamic
250 dex score of 3 or 4) and 1 with a detectable NS5B S282T mutation after sofosbuvir plus ribavirin ther
256 was infected with HCV that had more than 95% NS5B sequence homology with the HCV strains of the 3 cas
263 stigation of thiophene-2-carboxylic acid HCV NS5B site II inhibitors, guided by measurement of cell c
264 f a productive binary NS5B-RNA complex, here NS5B slides along the RNA template facilitating position
265 a high barrier to resistance, with no S282T NS5B substitution or phenotypic resistance detected in p
266 arrier to resistance; however, low-frequency NS5B substitutions associated with treatment failure wer
268 Additionally, mutations in C, E1, NS3, and NS5B synergistically promoted HCV production in the back
271 reveal a genetic interaction between p7 and NS5B that contributes to virion specific infectivity.
272 ions revealed a fully closed conformation of NS5B that may facilitate de novo initiation of RNA repli
273 identified three mutations in NS3, NS4A, and NS5B that permitted full-length J6 propagation and adapt
277 ation of a novel series of inhibitors of HCV NS5B, through the use of structure-based design applied
278 of 1H-quinazolin-4-one (QAZ) allosteric HCV NS5B thumb pocket 2 (TP-2) inhibitors was recently repor
279 to result from the inability of the mutated NS5B to optimally regulate Rb abundance and thereby modu
282 cleoside inhibitors (NNIs) of HCV polymerase NS5B to wild-type and resistant NS5B polymerase proteins
284 Deep-sequencing analysis confirmed that NS5B variants L159F and V321A emerged in a subset of pat
286 performed molecular dynamics simulations of NS5B variants with different C-terminal lengths in the p
287 cell-to-cell transmission for SA13/JFH1Core-NS5B viruses as well as viruses with only p7 and nonstru
288 oding non-structural proteins NS4B, NS5A and NS5B, we assessed the ability of peripheral immunization
289 us reports of very low proportions of active NS5B, we observed that under optimized conditions up to
292 taining mutations in the Rb-binding motif of NS5B which ablate the ability of HCV to regulate Rb.
294 side and nucleotide analogs that inhibit HCV NS5B, which led to the FDA approval of sofosbuvir in 201
296 on of association-dissociation events of HCV-NS5B with RNA, and also the direct monitoring of the int
297 hepatitis C virus genes (E1, E2, NS2, NS4B, NS5B), with an emphasis on including as many sequences w
298 strated that SA13/JFH1orig and SA13/JFH1Core-NS5B, with and without the E2 mutation T385P, displayed
299 tations in core (C), E1, NS2, NS3, NS5A, and NS5B, with the majority of mutations clustered in NS5A.
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