ライフサイエンスコーパス: NTPase

1 NTPase activities in mammalian reovirus cores were exami

2 NTPase first appears 12 h postinfection.

3 enetic approach to obtain a genealogy of 148 NTPase genes and reconstruct a scenario of gene superfam

4 Prior results show that the 1a NTPase/helicase-like domain contributes to RNA recruitme

5 to some double-stranded RNA viruses, the 1a NTPase/helicase-like domain may be involved in importing

6 ins with the gene order p38.3 (Nterm)-p39.6 (NTPase)-p18.6-p14.3 (VPg)-p19.2 (Pro)-p57.5 (Pol).

7 conserved mitotic-like apparatus in which a NTPase (ParA) displaces the partition complex.

8 Even after NTPase induction, these proteins were retained within cy

9 HXGPRT RNA-ribozyme construct did not alter NTPase expression nor compromise parasite replication.

10 In addition, an NTPase-defective DHX33 mutant (K94R) acted as a dominant

11 n RNA-dependent RNA polymerase (RdRP) and an NTPase VP4.

12 under conditions where NSP2 functions as an NTPase, its helix-destabilizing activity was less sensit

13 into nonstructural proteins that include an NTPase, VPg, protease, and RNA-dependent RNA polymerase.

14 The key component is an NTPase protein that cycles between nucleotide-dependent

15 One of these proteins, D5, is an NTPase that contains an N-terminal archaeoeukaryotic pri

16 en shown to form multimers and to possess an NTPase activity, but its precise function remains unclea

17 es three elements: a DNA centromere site, an NTPase, and a centromere-binding protein.

18 This study reveals for the first time an NTPase activity associated with a calicivirus-encoded pr

19 teinase domain and an RNA triphosphatase, an NTPase domain, and an RNA helicase in the C-terminal dom

20 Consistent with an NTPase activity, conserved residues predicted to be requ

21 with the protein, helix destabilization and NTPase, may function together.

22 hat the RNA-binding, helix-destabilizing and NTPase activities of tsE NSP2 were significantly less at

23 Previous studies demonstrated helicase and NTPase activities for DDX3X, but important biochemical f

24 s predicted with respect to RNA helicase and NTPase activities in vitro.

25 imization of the parameters for helicase and NTPase assays are expected to provide the starting point

26 ture, suggesting that the viral helicase and NTPase reactions are not coupled.

27 in 3 (NS3), with its protease, helicase, and NTPase enzymatic activities, plays a crucial role in vir

28 ents in Upf1p including zinc finger-like and NTPase domains, as well as all motifs common to members

29 agenesis studies showed that the primase and NTPase activities of the recombinant D5 protein could be

30 efine the functional domains of protease and NTPase/RNA helicase activities of NS3, full-length and a

31 The RNA triphosphatase and NTPase activities of baculovirus LEF-4 resemble those of

32 ces between arginine fingers of dUTPases and NTPases are explained on the basis of the unique chemist

33 erties of full-length NS3 (NS3FL)-associated NTPase, RTPase, and RNA helicase are presented.

34 yzoite-specific nucleoside triphosphatase (B-NTPase) promoters.

35 f the OB domain also increased DHX29's basal NTPase activity, but more importantly, abrogated the res

36 Besides NTPase and other RNA helicase consensus motifs, UPF1 and

37 Both NTPase and pyrophosphatase activities were enhanced at h

38 sis showed that this mutation abolished both NTPase and helicase activities of MLE but affected the a

39 ivity alone (motif II, DEYH to DEYA) or both NTPase and helicase activity (motif I, GKT to GAT and de

40 The DEAH-box NTPase Prp43 and its cofactors Ntr1 and Ntr2 form the NT

41 The DExH-box NTPase/helicase Prp22p plays two important roles in pre-

42 DEAD/H-box NTPases remodel the spliceosome at multiple steps during

43 and Mu) and combine a CDC6/ORC1-STAND clade NTPase domain with a C-terminal REase domain.

44 To date, many house-cleaning NTPases have been identified only on the basis of their

45 Both proteins contain a conserved NTPase domain that genetic studies have demonstrated is

46 rally and functionally links the cytoplasmic NTPases of the system with its outer membrane and pilus

47 ional change couples rearrangement of the (d)NTPase active site to additional hydrogen-bonding betwee

48 ngs represent the first report demonstrating NTPase/RNA helicase activity of the helicase domain of H

49 nding, helix unwinding, and Mg(2+)-dependent NTPase activities and play a crucial role in assembly of

50 e a manganese-dependent and cobalt-dependent NTPase activity intrinsic to CaCet1p.

51 ificity for NTP analogues, the DNA-dependent NTPase activity associated with the herpes primase-helic

52 n catalyzes its characteristic DNA-dependent NTPase activity, and can unwind duplex DNA substrates in

53 ion, and is a DNA helicase and DNA-dependent NTPase.

54 ation system, we show that the HIT-dependent NTPase activity of NSP2 is necessary for dsRNA synthesis

55 to E. coli Rho in terms of its RNA-dependent NTPase activity and its sensitivity to the Rho-specific

56 n of the putative RNA helicase/RNA-dependent NTPase family, is a splicing factor that functions late

57 The RNA-dependent NTPase Prp43 catalyzes dissociation of excised lariat-in

58 nce had on primase activity, ssDNA-dependent NTPase activity was essentially unaffected by changes in

59 ial DNA helicases are nucleic acid-dependent NTPases that play important roles in DNA replication, re

60 he DEXH-box family of nucleic acid-dependent NTPases.

61 When activated by dithiols, NTPase is one of the most potent apyrases known to date,

62 entially converts an ecto-apyrase to an ecto-NTPase.

63 nRNP protein (hnRNP C and hnRNP U) or either NTPase protein (NAT10 and GNL3L) induced telomere shorte

64 gregate DNA, the most common plasmid-encoded NTPases contain Walker-box and actin-like folds.

65 The genes encoding NTPase have been cloned.

66 ne resulted in an ecto-apyrase with enhanced NTPase activity, but diminished NDPase activity.

67 HEV Hel exhibited NTPase and RNA unwinding activities.

68 tivity, whereas the N-terminal part exhibits NTPase and RNA triphosphatase activity and is proposed t

69 uction ATPases with numerous domains) family NTPase followed by a series of LRRs, suggesting inherita

70 d that Tm-MazG has dual enzymatic functions, NTPase and pyrophosphatase, and that these two enzymatic

71 al CV amino acid motifs, including GXXGXGKT (NTPase), EYXEX (Vpg), GDCG (protease), and GLPSG and YGD

72 phatase (NTPase) assay to show that UL84 has NTPase activity, preferring UTP.

73 roteins that have not been suspected to have NTPase activity, including soluble adenylyl cyclases, ne

74 ural basis for the enzymatic activity of HCR-NTPase was further characterized by site-directed mutage

75 which further contributes to making the HCR-NTPase an attractive new target for further biochemical

76 aviviridae family contain conserved helicase/NTPase motifs in their homologous NS3 proteins.

77 To determine the role of the NS3 helicase/NTPase in the viral life cycle, deletion and point mutat

78 life cycle, the precise role of the helicase/NTPase in virus replication or whether it is essential f

79 deletion and point mutations in the helicase/NTPase motifs of the bovine viral diarrhea virus (BVDV)

80 an RNA-dependent RNA polymerase, a hexameric NTPase, and an auxiliary protein.

81 an RNA-dependent RNA polymerase, a hexameric NTPase, and an auxiliary protein.

82 an RNA-dependent RNA polymerase, a hexameric NTPase, and an auxiliary protein.

83 The cystoviral hexameric NTPase, P4, uses chemical energy to translocate single-s

84 ts of actin-activated heavy meromyosin (HMM) NTPase, the rates of NTP binding to myosin and actomyosi

85 rfamily of genes for putative NTP hydrolase (NTPase) proteins that are strikingly similar in structur

86 olysis common among secretion NTP hydrolase (NTPase) proteins.

87 Nucleoside triphosphate hydrolase (NTPase) is a very abundant protein secreted by the oblig

88 pe-tagged nucleoside triphosphate hydrolase (NTPase) were partially activated during immunoprecipitat

89 g the 5' nucleoside triphosphate hydrolases (NTPase).

90 s indicate that most of the immunodetectable NTPase is within the nucleus, a compartment proteins typ

91 These data implicate NTPase in an essential parasite function and suggest tha

92 roteins that are widely conserved, including NTPases and secretins, and on proteins that are system s

93 rms dynamic filaments that require an intact NTPase motif for their turnover in vivo.

94 bitory role in suppressing DHX29's intrinsic NTPase activity but was not essential for its 40S-stimul

95 mediates through the energy derived from its NTPase activity.

96 Btoxis encoding a putative FtsZ/tubulin-like NTPase called TubZ and DNA-binding protein called TubR h

97 struction of the early history of the P-loop NTPase fold includes the initial split into the common a

98 a monophyletic assemblage within the P-loop NTPase fold.

99 R-proteins are P-loop NTPase superfamily members, and current models describe

100 in contrast to other superclasses of P-loop NTPases (RecA-F1/F0, AAA+, helicases, ABC), GTPases do n

101 tif defines the Mrp/Nbp35 subclass of P-loop NTPases and is suspected to be involved in transient Fe-

102 In all analyzed genomes, the P-loop NTPases are the most abundant fold.

103 proteins are distinguished from other P-loop NTPases by the presence of unique sequence motifs associ

104 The essential P-loop NTPases Cfd1 and Nbp35 of the cytosolic iron-sulfur (Fe-

105 P-loop NTPases of the ApbC/Nbp35 family are involved in FeS pro

106 ve-site elements in myosins and other P-loop NTPases play critical roles in nucleotide binding and hy

107 nimals, Cfd1 and Nbp35 are homologous P-loop NTPases that form a heterotetrameric complex essential f

108 ional strand, catalytic E division of P-loop NTPases together with the AAA+ ATPases, RecA/helicase-re

109 dditional strand catalytic 'E' (ASCE) P-loop NTPases, GHL proteins, actin-fold enzymes and chaperonin

110 sly unrecognized, widespread class of P-loop NTPases.

111 consistent with their roles in other P-loop NTPases.

112 cleotide binding fold typical for the P-loop NTPases.

113 y consistent with the biochemistry of P-loop NTPases.

114 hermore, PPi is an inhibitor for the Tm-MazG NTPase activity.

115 e to Mg.ATP, they increased the rates and Me.NTPase activity of cross-linked acto-S1 and the fast com

116 The rates of force recovery and Me.NTPase activity were less than for Mg.ATP (by 40-80% and

117 Actomyosin-Subfragment 1 (acto-S1) Me.NTPase activity and Me.NDP release were monitored under

118 Unregulated actin-activated Mg-NTPase rates and actin sliding speed linearly increased

119 fsR-like transcription regulators) and NACHT NTPases (e.g. NAIP, TLP1, Het-E-1) that have been studie

120 The NS5 stimulation of NS3 NTPase was dose-dependent until an equimolar ratio was r

121 Therefore, the effect of NS5 on the NS3 NTPase activity was examined.

122 The results show that NS5 stimulated the NS3 NTPase and RTPase activities.

123 rotease, the NS3-4A serine protease, the NS3 NTPase/helicase, and the NS5B polymerase.

124 in at the P3 position for the NS1/2-3 (Nterm/NTPase) site confers significant influence on enzyme cat

125 The nuclear NTPase activity was not inhibited by vanadate, oligomyci

126 consistently found in the P-loop of numerous NTPase domains, where it stabilizes the substrate-bindin

127 Accumulation of NTPase mRNA in etiolated seedlings is stimulated by brie

128 e of the enzyme, a significant activation of NTPase activity was observed.

129 e of the enzyme, a significant activation of NTPase activity was observed.

130 Comparative analysis of NTPase and helicase activities of wild type nsP2 with en

131 Thus, the RNA loading and tight coupling of NTPase activity with RNA translocation in 8 P4 is due to

132 n synthesized and screened for inhibition of NTPase/helicase of the West Nile Virus (WNV).

133 atic reduction in the steady-state levels of NTPase.

134 ndings, suggest either that a single type of NTPase in cores is strongly influenced by two different

135 or that cores contain two different types of NTPase influenced by the two proteins.

136 nt Pdr5 is attributable to the uncoupling of NTPase activity and transport.

137 been synthesized as potential inhibitors of NTPases/helicases of Flaviviridae, including the West Ni

138 (R1) of BceSIV contains conserved motifs of NTPases and DNA helicases.

139 s not reported before in this superfamily of NTPases.

140 e full NS3-NS4A complex demonstrated optimal NTPase activity between pH 7.5 and 8.5.

141 5.7 kDa, which we designated p5.6, p32, p39 (NTPase), p30, p13 (VPg), and p76 (Pro-Pol), respectively

142 hree further highly divergent, cystoviral P4 NTPases (from 6, 8 and 13).

143 ic trees constructed for the NS polyprotein, NTPase, protease, polymerase, and capsid protein sequenc

144 ins needed for viroplasm assembly, possesses NTPase, RNA-binding, and helix-unwinding activities.

145 as for Maf, the structure of this predicted NTPase was determined as part of a structural genomics p

146 includes several other groups of (predicted) NTPase domains from diverse signaling and transcription

147 unctional enzyme possessing serine protease, NTPase, and RNA unwinding activities that are required f

148 ne of these, EpsE, is a cytoplasmic putative NTPase essential for the functioning of the Eps system a

149 or precisely classifying and naming putative NTPase genes based on phylogeny.

150 mily of the family encompassing all putative NTPases of type IV secretion systems.

151 tein, a member of a large family of putative NTPases from type II and IV secretion systems.

152 members of the PulE superfamily of putative NTPases that have extensive sequence similarity and prob

153 dA-like genes to those encoding the putative NTPases of type II/IV secretion, we used a phylogenetic

154 ucleoside triphosphate pyrophosphohydrolase (NTPase) and pyrophosphatase activities.

155 Ring NTPases of the ASCE superfamily perform a variety of cel

156 Ring NTPases represent a large and diverse group of proteins

157 nct subfamily of potential type IV secretion NTPase genes.

158 e hexameric rings of other type IV secretion NTPases.

159 binding was observed in many, and sequential NTPase catalysis has been observed in two proteins, gp4

160 protein BAG1 and a novel bradyzoite-specific NTPase during bradyzoite development were fine mapped to

161 sed a domain structure that included a STAND NTPase paired with a series of tetratricopeptide repeats

162 containing the common ancestor of the STAND NTPase domain of R-proteins and NLRs.

163 STAND NTPases represent a novel paradigm in signal transductio

164 n of the archaeal families, almost all STAND NTPases are multidomain proteins containing three or mor

165 Transfer of genes for STAND NTPases from bacteria to eukaryotes on several occasions

166 AAA+ ATPases, it can be predicted that STAND NTPases use the C-terminal helical bundle as a "lever" t

167 The STAND NTPases are most abundant in developmentally and organiz

168 aining the last common ancestor of the STAND NTPases of plant R-proteins and animal NLRs (and, by ext

169 Focusing on the STAND NTPases of plant R-proteins, animal NLRs, and their homo

170 A, HET-E, and TEP1) subfamilies of the STAND NTPases, we analyzed the phylogenetic distribution of th

171 but was not essential for its 40S-stimulated NTPase activity and function in initiation.

172 to E1, all NTPs tested support K+-stimulated NTPase activity and H+ pumping up to 30-50% of that with

173 uired for serine protease and RNA-stimulated NTPase activities map within the region between amino ac

174 The RNA-stimulated NTPase activity was significantly affected by deletion o

175 otease activity, a C-terminal RNA-stimulated NTPase activity, and an RNA helicase activity.

176 ays an important role for the RNA-stimulated NTPase activity.

177 gion of NS3 (NS3hel) exhibits RNA-stimulated NTPase and helicase activity, while the N-terminal serin

178 ental effect on the basal and RNA-stimulated NTPase as well as RNA helicase activities.

179 RNA replication factors: 1a has a C-terminal NTPase/helicase-like domain, and 2a(pol) has a central p

180 e domain structure, comprising an N-terminal NTPase domain and a C-terminal DUF1998 domain (containin

181 PrrCs consist of two domains: an N-terminal NTPase module related to the ABC family and a distinctiv

182 essential parasite function and suggest that NTPase may have more than one function in vivo.

183 The NTPase activity of this component, before and after sepa

184 The NTPase activity was necessary, but not sufficient, to su

185 The NTPase activity was not stimulated by single-stranded nu

186 The NTPase activity with deoxyribonucleoside triphosphates a

187 The NTPase as it occurs with the polymerase displays cleavag

188 The NTPase can be exploited to screen in vitro for inhibitor

189 The NTPase was stimulated more than 50% by red light, and th

190 ion by the polymerase and of cleavage by the NTPase, operating on the same substrate pool.

191 s moiety of the DExH-box serve to couple the NTPase and helicase activities.

192 en infected cells were treated with DTT, the NTPase was activated in a dose-response fashion, as asse

193 Hence the NTPase activity of NSP2 probably has a role subsequent t

194 Overexpressing PrrCs with mutations in the NTPase active site ameliorated the toxicity of wild-type

195 Significant decreases in the NTPase activity and concomitant increases in the pyropho

196 econdary binding site that is located in the NTPase domain (Domain II) of NS3.

197 , we identified 22 essential residues in the NTPase domain and 11 in the nuclease domain.

198 blotting with pea genomic DNA indicates the NTPase is likely to be encoded by a single gene.

199 bA bind to synthetase, but do not induce the NTPase activity.

200 binding to RNA substrate and modulating the NTPase/RNA helicase and RTPase activities of NS3.

201 f its Walker A nucleotide-binding motif, the NTPase activity of TssM and its role in T6SS remain obsc

202 In addition to hydrolyzing NTPs the NTPase could also hydrolyze the 5'-terminal phosphate fr

203 aluation of the biological importance of the NTPase activity of NSP2 by transient expression in mamma

204 Potential implications of the NTPase activity of PilT in pilus retraction are discusse

205 rtantly, abrogated the responsiveness of the NTPase activity to stimulation, which abolished DHX29's

206 activity in vitro, and thus the role of the NTPase domain in cluster biogenesis has remained uncerta

207 kelihood methods to infer a phylogeny of the NTPase domains of R-proteins, and reconstructed the doma

208 ntingent on head-to-tail dimerization of the NTPase domains to form two composite NTP phosphohydrolas

209 indicate that the cleavage preference of the NTPase for noncomplementary NTPs is not part of a mechan

210 has led to proposals for involvement of the NTPase in transcriptional error prevention.

211 unctions of the conserved amino acids of the NTPase motifs are context dependent.

212 e that the oxidation/reduction status of the NTPase, the only parasite dense granule protein that con

213 to examine the RNA helicase activity of the NTPase/helicase domain of HEV, the region (amino acids 9

214 xamine the effects of polynucleotides on the NTPase, helicase, and protease activities.

215 he pilus filament is extruded, and PilT, the NTPase that mediates pilin disassembly and retraction.

216 We have reexamined the NTPase activity of Nbp35, Cfd1, and their complex.

217 res the centromere-binding protein SopB, the NTPase SopA and the sopC centromere.

218 Northern blot analysis shows that the NTPase MRNA is strongly expressed in etiolated plumules,

219 These results indicate that the NTPase/helicase activities are essential functions of ML

220 In addition to the NTPase domain, these proteins typically contain DNA-bind

221 terminal helical bundle that is fused to the NTPase domain.

222 that IgG against human lamin C binds to the NTPase in immunoblots.

223 motif III (SAT) mutations that uncouple the NTPase and helicase activities.

224 hyperphosphorylation when combined with the NTPase and helix-destabilizing protein NSP2.

225 merase, which then does not compete with the NTPase for the substrate pool.

226 helicases, plays an important role in their NTPase cycle.

227 n, myosin and G proteins indicate that these NTPases may have a similar strategy of changing conforma

228 f 15 genes in the operon and has homology to NTPases of type IV secretion systems.

229 sed an associated nucleoside triphosphatase (NTPase) activity in vitro, which in the presence of Mg(2

230 a Mg2+-dependent nucleoside triphosphatase (NTPase) activity, and is a component of replication inte

231 es 40S-stimulated nucleoside triphosphatase (NTPase) activity.

232 the protein, and nucleoside triphosphatase (NTPase) and helicase activities reside in the remaining

233 Here, by nucleoside triphosphatase (NTPase) assay and comparisons of six high-resolution (2.

234 ed in an in vitro nucleoside triphosphatase (NTPase) assay to show that UL84 has NTPase activity, pre

235 The LEF-4 nucleoside triphosphatase (NTPase) is activated by manganese or cobalt but not by m

236 Helicase/nucleoside triphosphatase (NTPase) motifs have been identified in many RNA virus ge

237 A nucleoside triphosphatase (NTPase) present in highly purified preparations of pea n

238 encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuc

239 ral proteins with nucleoside triphosphatase (NTPase)/helicase motifs or activities.

240 an RNA-stimulated nucleoside triphosphatase (NTPase)/RNA helicase and a 5'-RNA triphosphatase (RTPase

241 al RNA-stimulated nucleoside triphosphatase (NTPase)/RNA helicases.

242 ciated nucleoside and 5' RNA triphosphatase (NTPase/RTPase) activities that are mediated by a conserv

243 A second S. cerevisiae RNA triphosphatase/NTPase (named Cth1p) containing motifs A, B, and C was i

244 cleotide binding nucleoside triphosphatases (NTPases) or nucleoside triphosphate (NTP) binding protei

245 c acid-dependent nucleoside triphosphatases (NTPases), which is defined by the presence of several co

246 ly of metal-dependent RNA 5'-triphosphatases/NTPases encoded by fungi and DNA viruses; the family is

247 e the hydrolysis of nucleoside triphosphate (NTPase) to nucleic acid unwinding.

248 tive and inactive telomerase RNPs, while two NTPase proteins associate preferentially with active enz

249 he data presented here suggest that the two "NTPase" catalytic sites in terminase holoenzyme communic

250 ailed to show any activity against the viral NTPase reaction even up to 500 muM.

251 itional allosteric binding site on the viral NTPases/helicases that can be occupied by nucleoside/nuc

252 helicase and ATPase activities of the viral NTPases/helicases.

253 us replication, insofar as mutants for which NTPase was uncoupled from unwinding (H299A, T326A, and T

254 RNA polymerase harbor a 70 kDa protein with NTPase (beta-gamma cleavage) activity that is not a reco