ライフサイエンスコーパス: Northern blot

1 the Pneumocystis life cycle was analyzed by Northern blot.

2 8, and 24 hrs in septic and control rats by Northern blot.

3 sitivity similar to small RNA sequencing and northern blots.

4 ; nine of these appeared to be stabilized on Northern blots.

5 mples from frontal and parietal cortex using Northern blots.

6 d cells using next-generation sequencing and northern blots.

7 press Slc34a1 sense/antisense transcripts by northern blotting.

8 eal-time polymerase chain reaction (PCR) and northern blotting.

9 was used to detect mRNA for the Lcn2 gene in Northern blotting.

10 nal for BPAG1 in the brain of dt-Alb mice by Northern blotting.

11 transcription polymerase chain reaction, and Northern blotting.

12 eq data and nine microRNAs were confirmed by northern blotting.

13 RNA sequencing, quantitative PCR (qPCR), and Northern blotting.

14 RNA samples and their subsequent analysis by Northern blotting.

15 enic tiling microarray and were confirmed by northern blotting.

16 Northern blot analyses also demonstrated a positive role

17 ld restore GADD45beta expression in HepG2 in Northern blot analyses and quantitative real-time polyme

18 Northern blot analyses confirmed the associated molecula

19 Northern blot analyses demonstrate that CvMT-IV is down-

20 Southern blot and Northern blot analyses demonstrated hybridization of a r

21 Northern blot analyses demonstrated that liver expressed

22 Northern blot analyses detected full-length replicon RNA

23 Transcriptional fusions and Northern blot analyses indicate that NanR represses the

24 Northern blot analyses indicate that SA suppresses GA-in

25 and polyamine transport and metabolism, and Northern blot analyses indicate that the AbcR sRNAs acce

26 In agreement, strand-specific RT-PCR and Northern blot analyses indicated that antisense transcri

27 Northern blot analyses of a subset of the neuronal genes

28 Northern blot analyses of infected-cell RNA showed that

29 Northern blot analyses of mitochondrial transcripts indi

30 Northern blot analyses provided evidence that three mRNA

31 Northern blot analyses revealed low expression levels of

32 Surprisingly, Northern blot analyses revealed that cidABC and lrgAB tr

33 Northern blot analyses revealed that the cidR mutation c

34 Northern blot analyses showed expression of EDIII antige

35 Furthermore, reverse transcriptase PCR and Northern blot analyses showed that cpb2 genes in all of

36 Microarray and Northern blot analyses showed that RR06 is specifically

37 Subsequent Northern blot analyses showed that the up-regulated tran

38 ure rodent microRNAs and quantitative RT-PCR/Northern blot analyses showed that Trx1 upregulates memb

39 onal analyses by transient transfections and Northern blot analyses suggest APBP-1 has the capacity t

40 Microarray and Northern blot analyses suggested that the expression of

41 In this study, we used microarray and Northern blot analyses to determine the S. pyogenes mRNA

42 oarray and/or quantitative real-time PCR and northern blot analyses, microRNA-218 (miR-218) was speci

43 oliferation and colony reduction assays, and Northern blot analyses, neither blocking of the binding

44 By Western and Northern blot analyses, NK1-FL and NK1-Tr were coexpress

45 ssed for immunocytochemistry and Western and Northern blot analyses, to determine stromal cell densit

46 Using 3'-rapid amplification cDNA end and Northern blot analyses, we identified the complete 3'-un

47 ion, Western blot, immunohistochemistry, and Northern blot analyses.

48 n as confirmed by transcriptional fusion and Northern blot analyses.

49 pairs identified in dsRNAs were confirmed by Northern blot analyses.

50 tive reverse transcription-PCR (qRT-PCR) and Northern blot analyses.

51 Several of these changes were confirmed by Northern blot analyses.

52 ression using quantitative real-time PCR and Northern blots analyses.

53 is study, we used proteomics and Western and Northern blotting analyses to demonstrate that alcohol d

54 roarray, reverse transcription (RT)-PCR, and Northern blotting analyses, we identified 11 virally enc

55 Northern-blot analyses performed on chloroplast transgen

56 Northern blot analysis and 3' rapid amplification of cDN

57 Northern blot analysis and 5' rapid amplification of cDN

58 Northern blot analysis and deep sequencing showed that i

59 Northern blot analysis and DNA binding studies of the pu

60 ally investigated six novel candidates using Northern blot analysis and found expression of three can

61 transcription-polymerase chain reaction and Northern blot analysis and of protein expression by West

62 Northern blot analysis and protein activity analysis dem

63 Using Northern blot analysis and transcriptional reporter gene

64 Northern blot analysis confirmed a broad inhibitory effe

65 Northern blot analysis confirmed expression of all miRNA

66 Northern blot analysis confirmed that interferon-gamma h

67 Northern blot analysis confirmed the expression of many

68 Northern blot analysis confirmed upregulation of JIP-1.

69 Northern blot analysis demonstrated a close correlation

70 ption polymerase chain reaction (RT-PCR) and northern blot analysis demonstrated reduced expression o

71 Northern blot analysis demonstrated that kat3 mRNA is wi

72 Microarray and Northern blot analysis demonstrated that under low oxyge

73 In situ hybridization and Northern blot analysis demonstrated the presence of endo

74 Northern blot analysis detected the siRNAs products in v

75 Northern blot analysis detected the stable 2.0-kb LAT in

76 Northern blot analysis disclosed that teg49 and teg48 we

77 Northern blot analysis followed by sequencing of B3 prog

78 the count of cell number for proliferation, Northern blot analysis for gene expression (up to 10 day

79 adult cadaveric donors and were assessed by Northern blot analysis for growth and melanogenic respon

80 Northern blot analysis found that Wnt-1 strongly induced

81 findings were validated by real-time PCR and Northern blot analysis in an independent panel of colon

82 This result was confirmed by Northern blot analysis in multiple models of dexamethaso

83 TaqMan and Northern blot analysis indicate BTNL2 is predominantly e

84 Northern blot analysis indicated that Cgap1p regulates t

85 Northern blot analysis indicated that each TbAK isoform

86 Northern blot analysis indicates that human VIP1 is expr

87 Northern blot analysis involves the separation of RNA mo

88 Northern blot analysis of adult brain also showed a sele

89 ementary tissues in the developing limb, and Northern blot analysis of chick limb bud mRNA shows that

90 Northern blot analysis of EBOV-infected cells using GP-g

91 ay, electrophoretic mobility shift assay and Northern blot analysis of expression of endogenous kappa

92 Northern blot analysis of extracts of renal cortical tis

93 Northern blot analysis of PvCYP707As in bean showed a hi

94 Northern blot analysis of representative mRNAs confirmed

95 Northern blot analysis of RNA encapsidation in vivo of t

96 Northern blot analysis of RNA extracted from cells follo

97 , site-directed mutagenesis experiments, and Northern blot analysis of RNA produced by constructs ind

98 Northern blot analysis of total RNA from the O46E parent

99 IGFBP expression was evaluated by RT-PCR and Northern blot analysis of total RNA preparations.

100 Northern blot analysis revealed 1.5 kb hMCA transcripts

101 al samples by quantitative real-time PCR and Northern blot analysis revealed an inverse correlation b

102 Northern blot analysis revealed an inverse mRNA expressi

103 Northern blot analysis revealed that both of these genes

104 Northern blot analysis revealed that Bryo-1 treatment of

105 Northern blot analysis revealed that the expression of M

106 Northern blot analysis revealed that these transcripts w

107 Northern blot analysis revealed the presence of endogeno

108 Northern blot analysis revealed the sup70-65 tRNA(Gln)(C

109 Both promoterless dicistronic test and northern blot analysis show transcripts being derived fr

110 Northern blot analysis showed a 4.5-kb transcript that i

111 Northern blot analysis showed that croaker HIF-1alpha an

112 Northern blot analysis showed that miR160 is abundant in

113 Northern blot analysis showed that the mRNA level of ost

114 In the present study, northern blot analysis showed that, also in humans, NCBE

115 2 cluster of snoRNAs by RNase protection and northern blot analysis shows that the MBII-52 expressing

116 However, Northern blot analysis suggests that IGFBP-5 is the pred

117 atabases, RT-PCR, in situ hybridization, and Northern blot analysis that cells in the mouse retina ex

118 Microarray expression experiments and northern blot analysis using RNA obtained from human ski

119 Quantitative polymerase chain reaction and northern blot analysis verified that bound mRNA remained

120 To confirm this, Northern blot analysis was performed and showed that the

121 m (ArsRS) in this acid-inducible regulation, Northern blot analysis was performed with RNAs isolated

122 Northern blot analysis was used to measure hepatic mRNA

123 Northern blot analysis was used to verify ZBED4 mRNA exp

124 Using northern blot analysis we demonstrate that L1 splicing,

125 small RNA substrates were probed directly by Northern blot analysis with RNA from the RNase P TS muta

126 Northern blot analysis with sense RNA and strand-specifi

127 rythroid-specific expression was detected by Northern blot analysis, and maximal expression during th

128 ed by nuclear in vitro transcription run-on, northern blot analysis, and quantitative polymerase chai

129 Based on Northern blot analysis, exposure of PEL cells to hypoxia

130 d during chondrocyte maturation, as shown by Northern blot analysis, in situ hybridization, and real-

131 NAs (miRNAs), six of which were confirmed by Northern blot analysis, leaving four as provisional.

132 multiple molecular techniques, which include Northern blot analysis, real-time PCR, miRNA microarray,

133 Northern blot analysis, semiquantitative reverse transcr

134 features of IMPDH1 in the retina, including Northern blot analysis, serial analysis of gene expressi

135 ing an miRNA microarray screen, confirmed by Northern blot analysis, we defined a set of seven miRNAs

136 Using patch-clamp and Northern blot analysis, we explored the regulation of bT

137 xamining the expression pattern of miRNAs by Northern blot analysis, we found that Drosophila miRNAs

138 Using Northern blot analysis, we identified that miR-1 is spec

139 By Northern blot analysis, we identified the expected 7.5-k

140 tion of the VEGF promoter were determined by Northern blot analysis, Western blot analysis, and use o

141 nscription and replication was determined by Northern blot analysis, which examined full-length antig

142 ription run-on assays, promoter studies, and Northern blot analysis, while stability of the COL1A1 an

143 of these eight novel miRNAs was confirmed by Northern blot analysis.

144 A species (NP, F, HN, and L) was examined by Northern blot analysis.

145 e LPS binding protein CD14 were confirmed by Northern blot analysis.

146 miRNAs is validated by quantitative PCR and northern blot analysis.

147 that miR-3676 and miR-4521 are tsRNAs using Northern blot analysis.

148 Preferential gene induction was verified by Northern blot analysis.

149 ceptor for alpha-MSH (MC1-R), as assessed by Northern blot analysis.

150 by cDNA microarray analysis and confirmed by Northern blot analysis.

151 The mRNA expression pattern was examined by Northern blot analysis.

152 fter Dex treatment, a result consistent with Northern blot analysis.

153 oop position and expression as determined by northern blot analysis.

154 te sRNAs, a third of which were validated by northern blot analysis.

155 Northern blotting analysis indicates that SRG is highly

156 rimer extension experiments, consistent with Northern blotting analysis.

157 in vivo primer extension experiments and by Northern blotting analysis.

158 ression was demonstrated in transformants by northern-blot analysis.

159 Northern blot and 5' rapid amplification of cDNA ends an

160 obtained by degenerative RT-PCR and used for Northern blot and 5'-RACE analysis.

161 Northern blot and fluorescence-activated cell-sorting an

162 sence of nat-siRNAs is supported not only by Northern blot and genetic analyses, but also by the fact

163 ogs, and their metabolites was studied using Northern blot and patch clamp recording.

164 Northern blot and primer extension analyses identified t

165 Northern blot and qualitative PCR analyses demonstrated

166 Northern blot and quantitative real-time PCR analyses co

167 y expressed miRNAs were further confirmed by Northern blot and quantitative real-time polymerase chai

168 c-Myb overexpression was verified by Northern blot and quantitative reverse transcription-pol

169 Northern blot and quantitative reverse transcription-pol

170 Expression analysis using Northern Blot and quantitative RT-PCR confirmed 2.5- to

171 essed RNAs, out of which 6 were confirmed by northern blot and RACE.

172 Northern blot and real time PCR analyses revealed an ele

173 Northern blot and real-time PCR analyses suggested that

174 n (ChIP) assays, and gene transactivation by Northern blot and reporter assays.

175 Using Northern blot and reporter replicon assays, we demonstra

176 Northern blot and reverse transcriptase PCR (RT-PCR) ana

177 Northern blot and reverse transcriptase-PCR studies indi

178 c tissues, including brain, as determined by Northern blot and reverse transcription-PCR analysis.

179 RNA could be identified in the cells by both Northern blot and RNase protection assay.

180 he presence of the variants was confirmed by Northern blot and RNase protection assays.

181 Northern blot and RT-PCR analyses confirmed an operon st

182 In silico as well as Northern blot and RT-PCR analyses indicated these genes

183 Northern blot and RT-qPCR experiments revealed specific

184 However, Northern blot and small RNA deep sequencing analyses ind

185 et of miRNAs with the results from small RNA Northern blot and that from miRNA quantitative RT-PCR.

186 Northern blot and transcription start site analyses reve

187 We confirmed this finding by Northern blot and Western blot analyses in cultured vasc

188 Both Northern blot and Western blot analyses were conducted o

189 hybridization, immunohistochemistry, RT-PCR, northern blot and western blot experiments.

190 hybridization, immunohistochemistry, RT-PCR, northern blot and western blot experiments.

191 Genes with altered expression verified by Northern blot and/or quantitative PCR were considered ca

192 ysregulated miRNAs were further confirmed by Northern blot and/or real-time polymerase chain reaction

193 thelin 1 in culture media were measured with Northern blots and enzyme immunoassays, respectively.

194 Northern blots and enzyme-linked immunosorbent assay (EL

195 Ss) and validate novel RNA transcripts using Northern blots and luciferase promoter fusions.

196 While Northern blots and promoter:GUS expression patterns have

197 miRNA and their precursors was evaluated by Northern blots and real-time polymerase chain reaction,

198 Northern blots and RT-PCR analysis of total RNA revealed

199 by Livny et al., we tested 40 candidates by Northern blotting and confirmed the expression of nine n

200 Northern blotting and fluorescence microscopy indicate t

201 Using Northern blotting and quantitative reverse transcriptase

202 Northern blotting and reverse transcriptase PCR demonstr

203 both in vivo immunoprecipitation followed by Northern blotting and RNA degradation assays, we confirm

204 Northern blotting and studies with a series of chromosom

205 , (ii) extracted for total RNA isolation for Northern blotting and, (iii) analysed immunohistochemica

206 opargyl-puromycin incorporation experiments, Northern blot, and electron microscopy analyses demonstr

207 scan analysis, differential display RT-PCR, Northern blot, and RT-PCR analyses were used to determin

208 with quantitative reverse transcription-PCR, Northern blot, and Western blot analyses, to characteriz

209 NA fluorescent in situ hybridization (FISH), Northern blots, and RNA sequencing-implicates MRP RNA in

210 and subgenomic RNA strands were detected by Northern blotting, and crystalline lattices of viral par

211 Pulse-chase, Northern blotting, and primer extension analyses in the

212 ghput deep RNA sequencing, quantitative PCR, Northern blotting, and rapid amplification of cDNA ends

213 Using transient co-transfections, northern blots, antisense oligonucleotides, and a histon

214 els of siRNAs that are readily detectable by Northern blot, are loaded into RNA-induced silencing com

215 ncing, computational analysis, and sensitive northern blot assays, we show that constitutively expres

216 his shortcoming by demonstrating multiplexed northern blotting based on the mechanism of hybridizatio

217 We describe a new northern blot-based protocol (LED) for small RNA (approx

218 Northern blotting can be done in 2 d, but detection of a

219 RNase protection and northern blot confirmed microarray results.

220 Real-time RT-PCR and Northern blots confirmed that established core Mga regul

221 n, 195 small RNAs (sRNAs) were detected, and Northern blots confirmed that many sRNAs were induced by

222 Northern blots confirmed that the increase of codon-opti

223 ent quantitative real-time PCR (qRT-PCR) and Northern blotting demonstrated that the ebpABC mRNA tran

224 iab-8 RNA also encodes a miRNA, detected on Northern blots, derived from the hairpin complementary t

225 Expression of ncRNAs was studied by northern blotting during (i) the normal developmental cy

226 When using northern blotting during these studies, we discovered th

227 Northern blots enable detection of a target RNA of inter

228 Ribonuclease protection assays, Northern blotting, enzyme-linked immunosorbent assay, an

229 Northern blotting established that HCV dsRNA contained g

230 Northern blot experiments revealed that the amount of ms

231 d the detection of miRNA by hybridization in northern blotting experiments.

232 Northern blotting for five of these genes (alpha-Catenin

233 Northern blotting for individual miRNAs showed that the

234 Northern blotting found TCR mRNA expression predominantl

235 Northern blotting, gene-specific RT-PCR, RNA pull-down a

236 Northern blot hybridization analysis revealed that hlyBA

237 Northern blot hybridization analysis showed that the trx

238 re primarily 24 nt in length, as detected by Northern blot hybridization and by sequencing small RNAs

239 The microarray results were confirmed by Northern blot hybridization and quantitative reverse-tra

240 e detected during infection of Vero cells by Northern blot hybridization.

241 o various noxious agents were examined using Northern blot, in situ hybridization, and immunohistoche

242 Northern blotting, in situ hybridization, and reporter g

243 ains, and transcript analysis (by RT-PCR and northern blotting) indicated the effect was mainly trans

244 and quantitative, the main disadvantage of a northern blot is that it detects such RNA less sensitive

245 Currently, northern blot is the most widely used method for validat

246 This improved northern blotting is particularly useful to analyze prod

247 e alpha-subunit gene expression, assessed by Northern blots, it stimulated a luciferase reporter plas

248 This protocol describes an improved northern blot method that enhances detection of small RN

249 We established, using Northern blotting, nuclear run-on assays, and RT-PCR, th

250 Furthermore, Northern blots of PNPase or RhlB mutants showed that Rhl

251 High resolution northern blots of subject fibroblast RNA suggested incom

252 small interfering RNAs (siRNAs) followed by Northern blotting of GAPDH, expression of N(pro) had no

253 alyzed by reverse transcription (RT)-PCR and Northern blotting of lens total RNA, immunoblotting of l

254 levated ocular transcripts were confirmed by Northern blotting of RNA from Bbs4(-/-) and three additi

255 aches that complement RNA-seq, discover that northern blotting of small RNAs is biased against short

256 our novel miRNAs (miR-x1- miR-x4), either by northern blot or by Ribonuclease Protection Assay.

257 t always be consistent with the results from Northern blot or miRNA quantitative RT-PCR.

258 ffers unique advantages for specificity over northern blots or other microarray-based expression prof

259 er to perform and more sensitive than either northern blotting or ribonuclease protection assays.

260 The northern blot, or RNA gel blot, is a widely used method

261 We identified four novel transcripts using Northern blotting, phage library screening, and 5' rapid

262 The HCR northern blot protocol takes approximately 1.5 days inde

263 Cells were analyzed by Western blotting, Northern blotting, pulse-chase analysis, and immunofluor

264 estern blot, nuclear in vitro transcription, Northern blot, qPCR, and promoter transactivation analys

265 ipt of the human liver-specific MIR122 using Northern blot, quantitative real-time polymerase chain r

266 Gene validation was done by Northern blot, quantitative reverse transcriptase-polyme

267 ethods used in detection of miRNAs including northern blotting, quantitative real time PCR (qRT-PCR)

268 y used to monitor gene expression, including Northern blotting, real-time polymerase chain reaction,

269 Northern blots revealed 1.2 kb and 3.2 kb mRNA species t

270 Northern blots revealed lower levels of Nmt2 expression

271 Northern blotting revealed a complex transcription patte

272 Northern blotting revealed a coordinated time-of-day-dep

273 While northern blotting revealed only these two mRNAs, both PC

274 nation of enzyme-linked immunosorbent assay, Northern blot, reverse transcriptase-polymerase chain re

275 Analyses of GFP fusions, Northern blotting, reverse transcription polymerase chai

276 Northern blotting, reverse transcription-PCR, and SMART-

277 tro translation, quantitative real-time PCR, Northern blot, ribonuclease protection assay, and monoci

278 Subsequent experiments using Northern blots, RT-PCR and real-time RT-PCR confirmed th

279 urified RNA can be used for analyses such as northern blotting, RT-PCR and microarrays.

280 examined TLR mRNA and protein expression by Northern blotting, RT-PCR, real-time RT-PCR, Western blo

281 uencing, and extended by a 5'-RACE assay and Northern blotting, showing that meiotic cells induce the

282 Northern blotting studies showed that the levels of the

283 nbiased expression data derived from a large Northern blot study.

284 Northern blots suggested that NF95 may be expressed at v

285 show by rapid amplification of cDNA ends and Northern blotting that Drosophila CCDC56 protein and mtT

286 We showed by using microarray analysis and Northern blotting that several genes are negatively regu

287 ocial evolution, we used deep sequencing and Northern blots to isolate caste-associated miRNAs in the

288 By northern blotting, two distinct mRNA transcripts of 5.9

289 Northern blotting using probes specific to hrcA, igr66 o

290 e found that the standard dose of UV used in northern blots was not the most efficient at immobilizin

291 Using Northern blot, we showed that the eNOS-derived 27-nt sho

292 By reporter gene fusion and Northern blotting, we found that sbcDC regulated capsule

293 Using in situ hybridization and Northern blotting, we observed the presence of endogenou

294 genes derived from other techniques (such as Northern blots) were used as reference genes in RT-qPCR

295 Keratin expression was studied by Northern blot, Western blot, and confocal microscopy.

296 By using Northern blot, Western blot, and immunofluorescent stain

297 enomedullary chromaffin cells as detected by Northern blotting, Western blotting, and specific PAI-1

298 Northern blots with RNA from adult mice and in situ hybr

299 Northern blotting with a CTV derived probe for assessmen

300 Northern blotting with comX probes revealed two distinct