ライフサイエンスコーパス: O(6)-methylguanine

1 key role in the interaction with the flipped O6-methylguanine.

2 e containing O6-benzylguanine and the other, O6-methylguanine.

3 le-stranded oligodeoxynucleotides containing O6-methylguanine.

4 most cytotoxic of lesions generated by TMZ, O(6)-methylguanine.

5 dem mass spectrometry analysis of unrepaired O(6)-methylguanine.

6 influenced by sequence context than that of O(6)-methylguanine.

7 , we examine the functions of this enzyme on O(6)-methylguanine (6mG) adducts in the four-stranded st

8 alkyltransferase (AGT) show that it forms an O(6)-methylguanine (6mG)-specific complex on duplex DNA

9 nucleotides containing a single well defined O(6)-methylguanine adduct were used to examine the exten

10 g-Cpr-null mice correlated with reduced lung O6-methylguanine adduct levels, without decreases in NNK

11 This work shows a mechanism whereby O(6)-methylguanine adducts formed by temozolomide lead t

12 The ability of temozolomide to form O(6)-methylguanine adducts is important for inhibition o

13 oma, causes cellular cytotoxicity by forming O(6)-methylguanine adducts.

14 NA alkyltransferase (AGT)-mediated repair of O6-methylguanine adducts.

15 ensitive to alkylating agents that result in O6-methylguanine adducts.

16 ficiently, whereas 7,8-dihydro-8-oxoguanine, O(6)-methylguanine, adenine, and guanine were not.

17 sensitized cells to the cytotoxic DNA lesion O(6)-methylguanine and caused a synthetic lethal interac

18 -1-(3-pyridyl)-1-butanone-induced short-term O(6)-methylguanine and long-term adenoma formation in th

19 o-N-nitrosoguanidine (MNNG), which generates O(6)-methylguanine and O(4)-methylthymine recognized by

20 lting in a fully functional protein for both O6-methylguanine and apurinic/apyrimidinic (AP) site rep

21 hesis across SOS-independent lesions such as O6-methylguanine and DNA uracil is around 90%, very clos

22 oplatinum(II), as well as base pairs between O6-methylguanine and thymine or cytosine, or between O4-

23 ecognize certain forms of DNA damage such as O6-methylguanine and UV photoproducts, and, therefore, m

24 ting a C residue opposite an abasic site, an O(6)-methylguanine, and an 8-oxoguanine lesion, respecti

25 inhibited the formation of N7-methylguanine, O6-methylguanine, and O6-pyridyloxobutylguanine at a nei

26 (c) bind to oligodeoxynucleotides containing O6-methylguanine; and (d) react with the low molecular w

27 action is the result of MutS recognition of O6-methylguanine base pairs.

28 However, the persistence of a premutagenic O(6)-methylguanine can also be invoked.

29 the protein is capable of tightly binding to O(6)-methylguanine-containing DNA and disrupting its rep

30 work showed that, in addition to changes in O(6)-methylguanine DNA methyltransferase (MGMT) activity

31 itative measurement of alkylation repair via O(6)-methylguanine DNA methyltransferase (MGMT) and base

32 O(6)-methylguanine DNA methyltransferase (MGMT) suppress

33 en used in this study to specifically target O(6)-methylguanine DNA methyltransferase (MGMT) to the m

34 id anchors capable of covalent attachment to O(6)-methylguanine DNA methyltransferase (SNAP-tag) fusi

35 MGMT (O(6)-methylguanine DNA methyltransferase) and APNG (alky

36 th LMO1 and MGMT, and thereby able to repair O6-methylguanine DNA adducts induced by MNU, would be pr

37 omain family protein 1A (RASSF1A) (57%), and O6-methylguanine DNA methylatransferase (MGMT) (34%), an

38 The human DNA repair protein O6-methylguanine DNA methyltransferase (MGMT) dealkylate

39 tin structure in the CpG island of the human O6-methylguanine DNA methyltransferase (MGMT) gene.

40 etinoic acid receptor-beta2 (RAR-beta2), and O6-methylguanine DNA methyltransferase (MGMT) genes were

41 O6-methylguanine DNA methyltransferase (MGMT) is a DNA r

42 of the gene encoding the DNA repair protein O6-methylguanine DNA methyltransferase (MGMT) might be r

43 ow cells express extremely low levels of the O6-methylguanine DNA methyltransferase (MGMT) protein th

44 The DNA repair protein O6-methylguanine DNA methyltransferase (MGMT) removes al

45 O6-Methylguanine DNA methyltransferase (MGMT) removes al

46 O6-Methylguanine DNA methyltransferase (MGMT) repairs th

47 tal carcinogens and the protective effect of O6-methylguanine DNA methyltransferase (MGMT), heterozyg

48 etection of four different proteins, avidin, O6-methylguanine DNA methyltransferase (MGMT), SNAP-tag,

49 complished in mammalian cells by the protein O6-methylguanine DNA methyltransferase (MGMT).

50 uclease protein resulted in the retention of O6-methylguanine DNA methyltransferase activity but loss

51 These results demonstrate that the fusion of O6-methylguanine DNA methyltransferase and apurinic endo

52 tructed a human fusion protein consisting of O6-methylguanine DNA methyltransferase coupled with an a

53 of the apurinic endonuclease portion of the O6-methylguanine DNA methyltransferase-apurinic endonucl

54 oviral-mediated delivery of the P140K mutant O(6)-methylguanine-DNA methyltransferase (MGMT(P140K)) i

55 er, gliomas expressing the DNA repair enzyme O(6)-methylguanine-DNA methyltransferase (MGMT) are resi

56 of the p16(INK4a) tumor suppressor gene and O(6)-methylguanine-DNA methyltransferase (MGMT) DNA repa

57 Patients with unmethylated O(6)-methylguanine-DNA methyltransferase (MGMT) fare wor

58 Expression of the DNA repair enzyme O(6)-methylguanine-DNA methyltransferase (MGMT) in tumor

59 O(6)-Methylguanine-DNA methyltransferase (MGMT) is a DNA

60 O(6)-methylguanine-DNA methyltransferase (MGMT) methylat

61 to elevated expression of the repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) or a def

62 Tumor tissue was assayed to determine O(6)-methylguanine-DNA methyltransferase (MGMT) promoter

63 O(6)-methylguanine-DNA methyltransferase (MGMT) repairs

64 The methylation status of the promoter of O(6)-methylguanine-DNA methyltransferase (MGMT) was asse

65 O(6)-Methylguanine-DNA methyltransferase (MGMT)(1), a ub

66 We have previously shown that O(6)-methylguanine-DNA methyltransferase (MGMT), a DNA r

67 in the context of the targeted knockdown of O(6)-methylguanine-DNA methyltransferase (MGMT), a DNA r

68 d and stabilized methyltransferases, chiefly O(6)-methylguanine-DNA methyltransferase (MGMT), a key e

69 or aberrant promoter methylation of the p16, O(6)-methylguanine-DNA methyltransferase (MGMT), death-a

70 n version of the DNA damage reversal protein O(6)-methylguanine-DNA methyltransferase (MGMT), which p

71 also required for the cytotoxic response of O(6)-methylguanine-DNA methyltransferase (MGMT)-deficien

72 irect reversal mechanism by a protein termed O(6)-methylguanine-DNA methyltransferase (MGMT).

73 in the normal cell by the DNA repair enzyme O(6)-methylguanine-DNA methyltransferase (MGMT).

74 ould be attributed to the down-regulation of O(6)-methylguanine-DNA methyltransferase activity.

75 INT1 (methylated in tumor 1), MINT2, MINT31, O(6)-methylguanine-DNA methyltransferase gene, and hMLH1

76 ion that for three genes (P16, MLH1, and the O(6)-methylguanine-DNA methyltransferase gene, MGMT), hi

77 of hepatocyte growth factor or unmethylated O(6)-methylguanine-DNA methyltransferase may benefit fro

78 vels of MET ligand hepatocyte growth factor, O(6)-methylguanine-DNA methyltransferase promoter methyl

79 ed glioblastoma that harbors a nonmethylated O(6)-methylguanine-DNA methyltransferase promotor, stand

80 D-54 tumors, which do not express measurable O(6)-methylguanine-DNA methyltransferase protein, is pro

81 The percentage of patients methylated at O(6)-methylguanine-DNA methyltransferase was lower than

82 ELF3, c-Jun, Rb2/p130, JAK1, p67phox, Grb2, O(6)-methylguanine-DNA methyltransferase, and Ercc-1.

83 yposis coli, APC; mut-L homolog 1, MLH1; and O(6)-methylguanine-DNA methyltransferase, MGMT) by liqui

84 some methylated bases from DNA, and suicidal O(6)-methylguanine-DNA methyltransferases to transfer al

85 HCT116 and HCT15 cells that highly express O(6)-methylguanine-DNA-methyltransferase (MGMT) displaye

86 h the latter fused to the DNA repair protein O(6)-methylguanine-DNA-methyltransferase (MGMT).

87 been identified in GBMs, except for loss of O(6-)methylguanine-DNA methyltransferase via promoter me

88 t increased levels of the DNA repair protein O6 methylguanine-DNA methyltransferase (MGMT), also refe

89 ructure-function information about the human O6-methylguanine-DNA methyltransferase (EC 2.1.1.63), as

90 Pretreatment leukemia cell O6-methylguanine-DNA methyltransferase (MGMT) activity,

91 ith a vector containing or lacking the human O6-methylguanine-DNA methyltransferase (MGMT) cDNA.

92 The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) confers re

93 h-level expression of the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) correlates

94 O6-methylguanine-DNA methyltransferase (MGMT) functions

95 The DNA-repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) inhibits t

96 The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) is a major

97 The DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) is commonl

98 The mechanism whereby the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) is silence

99 ed methylation data from a CpG island in the O6-methylguanine-DNA methyltransferase (MGMT) promoter.

100 nation in the disposition of the inactivated O6-methylguanine-DNA methyltransferase (MGMT) protein in

101 The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) removes mu

102 O6-Methylguanine-DNA methyltransferase (MGMT), a constit

103 O6-methylguanine-DNA methyltransferase (MGMT), a ubiquit

104 O6-Methylguanine-DNA methyltransferase (MGMT), a ubiquit

105 O6-Methylguanine-DNA methyltransferase (MGMT), an enzyme

106 This lesion is repaired by O6-methylguanine-DNA methyltransferase (MGMT), the expre

107 rivatives is countered by the repair protein O6-methylguanine-DNA methyltransferase (MGMT), which rem

108 ese mechanisms may be the high expression of O6-methylguanine-DNA methyltransferase (MGMT).

109 O6-MeG, a DNA lesion repaired by the protein O6-methylguanine-DNA methyltransferase (MGMT).

110 as the P140K mutant of the DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT*).

111 ariate analysis, the factors age, WHO grade, O6-methylguanine-DNA methyltransferase promoter methylat

112 wed prognostic significance, with WHO grade, O6-methylguanine-DNA methyltransferase status, age, and

113 ctional 39 kDa Escherichia coli Ada protein (O6-methylguanine-DNA methyltransferase) (EC 2.1.1.63), p

114 ges in molecules involved in DNA repair (eg, O6-methylguanine-DNA methyltransferase, DNA topoisomeras

115 DNA repair enzymes, DNA polymerase beta and O6-methylguanine-DNA methyltransferase, have been shown

116 cosylase, Nth protein (endonuclease III) and O6-methylguanine-DNA methyltransferase.

117 ve DNA lesion, O6-methylguanine (O6-MeG), by O6-methylguanine-DNA-methyltransferase (E.C. 2.1.1.63, M

118 essor gene p16 (CDKN2A), the DNA repair gene O6-methylguanine-DNA-methyltransferase (MGMT) and the pu

119 These include the importance of O6-methylguanine-DNA-methyltransferase (MGMT) in gliobla

120 r of metalloproteinase 3 (TIMP-3), p16INK4a, O6-methylguanine-DNA-methyltransferase (MGMT), death-ass

121 O6-methylguanine-DNA-methyltransferase (MGMT), which rep

122 uced cells in vivo, selection based on P140K O6-methylguanine-DNA-methyltransferase (MGMT[P140K]) gen

123 or radiotherapy alone, with consideration of O6-methylguanine-DNA-methyltransferase gene (MGMT) promo

124 ion-specific PCR and included: p16 (CDKN2A), O6-methylguanine-DNA-methyltransferase, glutathione S-tr

125 ne S-transferase P1, and the DNA repair gene O6-methylguanine-DNA-methyltransferase.

126 udied: 27% (26/95) at p16, 33% (31 of 95) at O6-methylguanine-DNA-methyltransferase; and 18% (17 of 9

127 otoxic effects of the TMZ-induced DNA lesion O(6)-methylguanine due to elevated expression of the rep

128 methylating agent MNNG to create a level of O(6)-methylguanine in cellular DNA equal to that found i

129 Temozolomide (TMZ) produces O(6)-methylguanine in DNA, which in turn mispairs with t

130 mutations arise as a result of formation of O(6)-methylguanine in DNA.

131 protein does not transfer methyl groups from O6-methylguanine in [3H]-methylated DNA but reversibly i

132 TMZ produces O6-methylguanine in DNA, which mispairs with thymine dur

133 n the alkyltransferase activity in repairing O6-methylguanine in methylated DNA.

134 over of MGMT protein and increased repair of O6-methylguanine in nitrosomethylurea-treated human bron

135 O6-Methylguanine is removed from DNA via the transfer of

136 C opposite this lesion, and it bypasses the O(6)-methylguanine lesion by inserting a C or a T.

137 In contrast, O(6)-methylguanine lesion used as a control does not ind

138 ia coli and purified to homogeneity repaired O(6)-methylguanine lesions in DNA via alkyl transfer act

139 r contributor to the endogenous formation of O(6)-methylguanine lesions in E. coli.

140 Interestingly, O(6)-methylguanine lesions when paired with either a C o

141 repair of the cytotoxic and mutagenic lesion O(6)-methylguanine (m(6)G) in DNA.

142 ir, and replication of a DNA lesion, such as O(6)-methylguanine (m(6)G), can, in principle, be influe

143 4)-methylthymine (m(4)T) relative to that of O(6)-methylguanine (m(6)G).

144 Replication in vivo across unrepaired O6-methylguanine (m6dG) lesions by mammalian DNA polymer

145 DNA synthesis in vitro across site-specific O6-methylguanine (m6dG) residues by DNA polymerase beta

146 O(6)-Methylguanine (m6G) and abasic site TLS was examine

147 O(6)-Methylguanine (m6G) is formed by the action of alky

148 gh the 7,8-dihydro-8-oxoguanine (8-oxoG) and O(6)-methylguanine (m6G) lesions.

149 We have studied the processing of O(6)-methylguanine (m6G)-containing oligonucleotides and

150 o form specific and stable complexes with an O6-methylguanine (m6G)-containing oligonucleotide substr

151 ng by Sn1 methylators has been attributed to O6-methylguanine (MeG), we have constructed nicked circu

152 n the course of removing a methyl group from O6-methylguanine (meG)-DNA or O4-methylthymine (meT)-DNA

153 lguanine DNA alkyltransferase (AGT) from the O(6)-methylguanine methyltransferase (MGMT) cDNA, which

154 K4a), retinoic acid receptor beta (RARbeta), O(6)-methylguanine methyltransferase (MGMT), and human m

155 The MutSDelta800 protein binds to O6-methylguanine mismatches but not to intrastrand plati

156 O6-methylguanine mispairs with thymine during replicatio

157 study, DNA adducts of biological relevance, O(6)-methylguanine (O(6)-MeG) and O(6)-carboxymethylguan

158 O(6)-Methylguanine (O(6)-MeG) is a highly mutagenic DNA

159 O(6)-Methylguanine (O(6)-meG) is a major mutagenic, carc

160 O(6)-methylguanine (O(6)-MeG) is a miscoding DNA lesion

161 ificance of the signaling pathway induced by O(6)-methylguanine (O(6)-MeG) lesions is poorly understo

162 leotides containing the known MGMT substrate O(6)-methylguanine (O(6)-MeG) or O(6)-CMG effectively in

163 icus DNA polymerase Dpo4-catalyzed bypass of O(6)-methylguanine (O(6)-MeG) proceeds largely in an acc

164 ate (MAMAc), and 7-methylguanine (7-MeG) and O(6)-methylguanine (O(6)-MeG) were measured in the DNAs

165 of primer/templates containing guanine (G), O(6)-methylguanine (O(6)-MeG), or O(6)-benzylguanine (O(

166 primer/template DNA containing guanine (G), O(6)-methylguanine (O(6)-MeG), or O(6)-benzylguanine (O(

167 O(6)-Methylguanine (O(6)-meG), which is produced in DNA

168 hibited formation of the promutagenic adduct O(6)-methylguanine (O(6)-meGua) by 73 and 80%, respectiv

169 O(6)-methylguanine (O(6)-methylG) is highly mutagenic an

170 methylating agents, which produce cytotoxic O(6)-methylguanine (O(6)-mG) DNA lesions.

171 Previous studies indicated that O(6)-methylguanine (O(6)-mG) persistence is critical for

172 s, including methylating agents that produce O(6)-methylguanine (O(6)MeG) adducts.

173 cytotoxic, and we recently demonstrated that O(6)-methylguanine (O(6)MeG) and O(6)-chloroethylguanine

174 O(6)-methylguanine (O(6)meG) and related modifications o

175 Alkylation-induced O(6)-methylguanine (O(6)MeG) DNA lesions can be mutageni

176 ponse to a single type of DNA lesion, namely O(6)-methylguanine (O(6)MeG).

177 O(6)-methylguanine (O(6)mG) is a potent mutagenic and pr

178 MNU produces carcinogenic O6-methylguanine (O6-meG) adducts, resulting in thymic l

179 nsformation of human fibroblasts and whether O6-methylguanine (O6-MeG) is involved, two populations o

180 We examined the effect of a single O6-methylguanine (O6-MeG) template residue on catalysis

181 Uracil, O6-methylguanine (O6-meG), and 8-oxoguanine (8-oxoG) wer

182 the repair of the chemosensitive DNA lesion, O6-methylguanine (O6-MeG), by O6-methylguanine-DNA-methy

183 of the O6 position of guanine in DNA to form O6-methylguanine (O6-meG).

184 t slowly, T opposite the carcinogenic lesion O6-methylguanine (O6MeG) approximately 30-fold more freq

185 e and a monoclonal antibody which recognizes O6-methylguanine (O6MeGua).

186 Ada protein makes critical interactions with O6-methylguanine (O6mG) at the N1- and O6-positions.

187 ts with the 3-position of the mutagenic base O6-methylguanine (O6mG).

188 tosine and 1,N6-ethenoadenine, as well as at O6-methylguanine (O6mG).

189 hase basicity (GB)) of adenine, guanine, and O(6)-methylguanine (OMG) have been examined using both t

190 We also examined O(6)-methylguanine (OMG), a highly mutagenic damaged bas

191 The effects of substitution of O6-methylguanine on the structure and stability of a hum

192 damaged DNA containing the endogenous lesion O(6)-methylguanine or cigarette-smoke-derived O(6)-4-(3-

193 PvuII is inhibited by O6-methylguanine, positioned within the restriction site

194 AGT repairs O6-methylguanine; PvuII cleaves at its restriction site,

195 We quantified the number of O6-methylguanine residues in methylated DNA by HPLC-MS/M

196 thyltransferase compete for the MNNG-induced O6-methylguanine residues, and MMR-induced cytotoxicity

197 These results suggest that O(6)-methylguanine uniquely activates the molecular swit

198 16-mer oligodeoxyribonucleotides containing O(6)-methylguanine, where a minimum of four nucleotides