ライフサイエンスコーパス: OATP2

1 (+)-independent organic anion transporter 2 (Oatp2).

2 increased hepatic expression of Oatp1 and/or Oatp2.

3 may function as intracellular substrates for Oatp2.

4 .6-kilobase (kb) 5'-flanking region of mouse Oatp2.

5 te cotransporting polypeptide (NTCP), OATP1, OATP2, ABCG5, and ABCG8) in the liver.

6 lot analysis revealed abundant expression of OATP2 after incubation of cells for 48 h in zinc, wherea

7 Cis-inhibition studies indicate that both OATP2 and roatp1 also transport other statins including

8 PXR target genes in liver, including CYP3A, Oatp2, and UGT1A1.

9 In addition, this study identifies rat oatp2 as a direct PXR-targeted gene and further supports

10 Furthermore, the identification of oatp1 and OATP2 as pravastatin transporters suggests that they are

11 These results suggest that Oatp2, but not Oatp1, is inducible by PB and PCN, which

12 Es are required for maximal induction of rat oatp2 by PCN.

13 ing exon gives rise to the two published rat oatp2 cDNAs.

14 Furthermore, unlike other subtypes, OATP2 did not transport digoxin or aldosterone.

15 In OATP2-expressing (zinc-induced) HeLa cells at 37 degrees

16 Interestingly, [(3)H]taurocholate uptake in Oatp2-expressing oocytes was also trans-stimulated when

17 ke of [(3)H]taurocholate or [(3)H]digoxin in Oatp2-expressing oocytes, indicating that the stimulator

18 ]S-(2,4-dinitrophenyl)-glutathione efflux in Oatp2-expressing oocytes.

19 1, uptake of 10 microM [(3)H]taurocholate in Oatp2-expressing Xenopus laevis oocytes was trans-stimul

20 in cell-associated [3H]bilirubin in induced (OATP2-expressing) as compared with uninduced cells (11.2

21 In conclusion, induction of Oatp2 expression in liver of fasted mice may be caused b

22 at fasting resulted in a 2-fold induction of Oatp2 expression in liver of mice.

23 The finding that Oatp2 expression increases in response to PB and PCN is

24 livers were extracted at various times, and Oatp2 expression was analyzed.

25 Oatp2 expression was suppressed in response to 3-MC.

26 Increases in Oatp2 expression were observed from livers of rats treat

27 The rat oatp2 gene consists of 16 exons; alternative splicing of

28 mechanism by which PCN treatment induces rat oatp2 gene expression.

29 ceptor (PXR), significantly enhances the rat oatp2 gene expression.

30 lar mechanism of oatp2 induction by PCN, rat oatp2 gene was cloned.

31 rs (bp) of the 5'-flanking region of the rat oatp2 gene were linked to the luciferase reporter gene a

32 ntified in the 5'-flanking region of the rat oatp2 gene.

33 A novel human organic transporter, OATP2, has been identified that transports taurocholic a

34 s other members of the OATP family, i.e. rat oatp2, human OATP, and the prostaglandin transporter, di

35 the current study indicates that a role for OATP2 in hepatocyte bilirubin transport is unlikely, it

36 nd organic anion-transporting polypeptide 2 (Oatp2) in liver in mice.

37 To understand the molecular mechanism of oatp2 induction by PCN, rat oatp2 gene was cloned.

38 during fasting, the role of this pathway in Oatp2 induction during fasting was examined.

39 Rodent Oatp2 is a hepatic uptake transporter for such compounds

40 In summary, OATP2 is a novel organic anion transport protein that ha

41 The present study examined whether rat Oatp2 is also an anion exchanger, and, if so, whether it

42 OATP2 is considerably diverged from other family members

43 OATP2 is expressed exclusively in liver in contrast to a

44 Taken together, the results indicate that Oatp2 mediates bidirectional transport of organic anions

45 alyses were used to assess hepatic Oatp1 and Oatp2 mRNA and protein, respectively.

46 N, in which PCN caused a robust elevation of Oatp2 mRNA and protein.

47 gs, dibutyryl cAMP and 8-bromo-cAMP, induced Oatp2 mRNA expression in a time- and dose-dependent mann

48 Maximal expression of Oatp2 mRNA was observed at 24 hours and remained elevate

49 Organic anion transporting polypeptide 2 (Oatp2) mRNA was decreased in pregnancy and increased pos

50 Luciferase activity driven by the Oatp2 promoter containing this CRE site was induced in c

51 -deletion constructs derived from the 7.6-kb Oatp2 promoter reporter gene construct, as well as 7.6-k

52 and remained elevated, whereas the amount of Oatp2 protein increased throughout the 96-hour interval.

53 ncy and increased postpartum, but changes in Oatp2 protein were not significant.

54 ffects of PCN treatment on the expression of Oatp2, rats were administered PCN, livers were extracted

55 ansporting polypeptides, Oatp1 (Slc21a1) and Oatp2 (Slc21a5), mediate hepatic uptake of cardiac glyco

56 at organic anion transporting polypeptide 2 (oatp2; Slc21a5) is a liver transporter that mediates the

57 A recent study suggests that human OATP2 (SLC21A6), also known as OATP-C and LST1, mediates

58 ively, n = 5) We obtained similar results in OATP2-transfected HEK293 cells that were used in the ori

59 cells that had been stably transfected with OATP2 under regulation of a metallothionein promoter.

60 r, organic anion transporting polypeptide 2 (Oatp2), was not affected.