ライフサイエンスコーパス: OCT2

1 f threonine 223 in the DNA-binding domain of OCT2.

2 n efficacy maximally in cells overexpressing OCT2.

3 d not significantly change the properties of OCT2.

4 D) values for binding to the model of rabbit OCT2.

5 Catfish Oct2 a and beta are tissue restricted, bind both consens

6 attenuated by saracatinib via inhibition of OCT2, a potential consideration for the clinical develop

7 In contrast, mouse Oct2 activated transcription in mouse but not catfish ce

8 Yes1 inhibition in vivo diminished OCT2 activity, significantly mitigating oxaliplatin-indu

9 Catfish Oct2 alpha and beta isoforms are derived by alternative

10 a more potent transcriptional activator than Oct2 alpha.

11 Mouse Oct2 also showed increased activity with the variant oct

12 one genes HIST1H1 B-E (27%) and mutations in OCT2 (also known as POU2F2; 8%), IRF8 (6%), and ARID1A (

13 Here, we show that cellular factors OCT2 and HCF1 bind OriP in association with EBNA1 to mai

14 cell lines are addicted to the expression of OCT2 and its coactivator OCA-B.

15 Finally, the transcriptional activator Oct2 and its cofactor OBF-1 were identified as regulator

16 c decreases in the kidney mRNA expression of Oct2 and Mate1, whereas Oct1 mRNA expression was only de

17 es of exogenous organic cation transporters (OCT2 and OCT3), organic anion transporter (OAT1), and mo

18 scriptase-polymerase chain reaction detected OCT2 and OCT3, but not OCT1, mRNA expression in CP.

19 redominantly B-cell-restricted expression of OCT2 and the absence of a systemic phenotype in our knoc

20 hylsulfonate, reduced transport by wild-type OCT2 and the mutant with cysteine 474 restored.

21 pitating and reducing transport by wild-type OCT2 and the mutant with cysteine 474 restored.

22 y through active secretion mediated by Oct1, Oct2, and Mate1.

23 in cells overexpressing mouse Oct2 or human OCT2, and this process was associated with increased DNA

24 OCT1 and OCT2 are involved in renal secretion of cationic drugs.

25 d in OCT1 orthologs as one amino acid and in OCT2 as a different one, influence homolog-specific sele

26 ues on the activity of the human ortholog of OCT2, as expressed in Chinese hamster ovary-K1 cells.

27 hypothetical three-dimensional structure of OCT2 based on a homology model that used the Escherichia

28 Catfish Oct2 beta is a more potent transcriptional activator tha

29 In transient expression assays, catfish Oct2 beta showed a marked preference for the octamer var

30 The POU transcription factors Oct1 and Oct2 bind to DNA in various monomer and dimer configurat

31 is competition assays indicated that catfish Oct2 binds the consensus octamer motif with an apparentl

32 POU homeodomain transcription factors Oct1, Oct2, Brn4, SCIP, Skn1a or Skn1i, results in a strong su

33 y of OCT3 is different from that of OCT1 and OCT2 but correlates significantly with that of the extra

34 To test this hypothesis, two catfish Oct2 cDNAs (alpha and beta) were cloned by screening a c

35 Our data indicate that the PORE-type Oct1 or Oct2 dimer, rather than the monomer, is the primary targ

36 and reduced tetraethylammonium transport by OCT2 expressed in Chinese hamster ovary cells, effects t

37 ng force for choline uptake in rat and human OCT2-expressing oocytes and in intact CP in vitro.

38 These findings indicate that HCF1 and OCT2 function at OriP to regulate viral transcription, h

39 model of the three-dimensional structure of OCT2, Glu(447) was found in a putative docking region wi

40 of multiple octamer motifs suggests that an Oct2 homologue may play an important role in driving exp

41 cells are formed normally after depletion of OCT2 in a conditional knockout mouse, but their prolifer

42 This finding led us to examine the role of OCT2 in germinal center-derived lymphomas.

43 Expression of OCT1 and OCT2 in Xenopus oocytes increased hemicholinium-3-sensit

44 istinct molecular properties of MATE1 versus OCT2 inhibitors and was used to screen the DrugBank in s

45 cal properties that differ between MATE1 and OCT2 inhibitors.

46 plicing; as determined by Southern analysis, Oct2 is a single copy gene.

47 These data demonstrate that cysteine 474 of OCT2 is exposed to the aqueous milieu of the cleft and c

48 Importantly, genetic alteration of OCT2 is not a requirement for cellular addiction in DLBC

49 found that the organic cation transporter 2 (OCT2) is expressed on dorsal root ganglia cells within t

50 comparisons with mammalian Oct2, the catfish Oct2 isoforms show high sequence conservation in their N

51 subtly alters the DNA-binding preference of OCT2, leading to the transactivation of noncanonical tar

52 We measured transport kinetics of OCT2-mediated uptake and demonstrated that IRIP overexpr

53 These data indicate that OCT2 mediates choline transport across the ventricular m

54 led expression of OCT3 mRNA, but not OCT1 or OCT2 mRNA, in the medial hypothalamus.

55 ly cloned organic cation transporters (OCT1, OCT2, NKT, NLT, RST, and OCTN1).

56 ntroducing mutations designed to disrupt the OCT2-OCA-B interface, we reveal a requirement for this p

57 uirement for the B-cell transcription factor OCT2 (octamer-binding protein 2, encoded by Pou2f2) in g

58 ransferase complex, and transcription factor OCT2 (octamer-binding transcription factor 2) bound coop

59 Depletion of OCT2 or HCF1 deregulated latency transcription and histo

60 16- to 35-fold in cells overexpressing mouse Oct2 or human OCT2, and this process was associated with

61 Along with OCT2, other SLC-family drug transporters are potentially

62 The organic cation transporter, OCT2, plays a role in renal secretion of a broad array o

63 ifs were examined to determine if they bound Oct2 POU domains in monomeric or dimeric (PORE and MORE)

64 ation, the two octamer motifs in Emu3' bound Oct2 POU domains only in monomeric configuration.

65 Mutations in OCT2 (POU2F2) affected its transcriptional and functiona

66 ermore, genetic or pharmacologic knockout of Oct2 protected mice from hypersensitivity to cold or mec

67 Ablation of OCT1 and OCT2 significantly reduced the distribution of metformin

68 anion transporters Slc22a1 (Oct1), Slc22a2 (Oct2), Slc22a6 (Oat1), Slc22a8 (Oat3), and Slc47a1 (Mate

69 notype in our knockout mice, suggest that an OCT2-targeted therapeutic strategy would be efficacious

70 The unique cell-cycle and OCT2-targeting activities of palbociclib and LEE011, com

71 e broad transcriptional program regulated by OCT2 that includes the expression of STAT3, IL-10, ELL2,

72 In comparisons with mammalian Oct2, the catfish Oct2 isoforms show high sequence conse

73 some other multidrug transporters, including OCT2, the results suggest that substrate identity exerts

74 The N353L and R403I substitutions (OCT2 to OCT1) did not significantly change the propertie

75 se Yes1, which was found to be essential for OCT2 tyrosine phosphorylation and function.

76 residues contained in the human ortholog of OCT2 was examined.

77 While catfish Oct2 was shown to be capable of binding PORE and MORE mo

78 ounds plays a role in their interaction with OCT2, we examined the influence of external pH values on

79 Catfish Oct2, when bound in this monomeric conformation, was sho

80 inhibitors of organic cation transporter 2 (OCT2), which contributes to the cellular accumulation of