ライフサイエンスコーパス: Oxytricha

1 ber in the developing nucleus of the ciliate Oxytricha.

2 DNA repeats from vertebrates, Tetrahymena or Oxytricha.

3 Telomeric sequences investigated include the Oxytricha 3' overhang, d(T4G4)2, and the related sequenc

4 n organizing guide for DNA rearrangements in Oxytricha and a template that can transmit spontaneous m

5 the macronuclear telomeres from the ciliates Oxytricha and Euplotes.

6 Oxytricha and its close relatives have a unique genome a

7 ciliates-Paramecium, Tetrahymena, Euplotes, Oxytricha and Stylonychia-reveal considerable variation

8 Recently, telomeric DNA in human, mouse, oxytricha, and trypanosome chromosomes have been found a

9 t of the telomere repeats of Tetrahymena and Oxytricha as well as that of the thrombin binding aptame

10 Here we use the single-celled ciliate, Oxytricha, as an analog to some of the genetic and genom

11 This study suggests a new important role in Oxytricha for this large portion of genomic DNA that was

12 Using a draft Oxytricha genome assembly and a custom-written protein-c

13 Here, we report the Oxytricha germline genome and compare it to the somatic

14 non-coding nanochromosomes, suggesting that Oxytricha has few independent ncRNA genes besides homolo

15 The assembly of the highly fragmented Oxytricha macronuclear genome is the first completed gen

16 The Oxytricha macronuclear TERT gene has no introns, whereas

17 For example, Oxytricha maintains tens of millions of telomeres per ce

18 ocesses related to the genomes and nuclei of Oxytricha may exemplify primitive states of the developi

19 ed DNA molecule in the macronucleus (mac) of Oxytricha nova (On) encoding heat-shock protein 70 (Hsp7

20 its of the telomere end binding protein from Oxytricha nova (OnTEBP) combine with telomere single str

21 tructure of the germline DNA polalphagene in Oxytricha nova .

22 de Pot1 in humans and fission yeast, TEBP in Oxytricha nova and Cdc13 in budding yeast.

23 s of 1.5 units of the repeat in telomeres of Oxytricha nova and has been shown by NMR and X-ray cryst

24 ats of the telomeric DNA sequence d(T4G4) of Oxytricha nova are capable of forming unusually stable s

25 he heterodimeric telomere binding protein of Oxytricha nova have been probed by Raman spectroscopy, C

26 0 000 IESs from a haploid germline genome in Oxytricha nova requires approximately 150 000 recombinan

27 assium-containing quadruplex formed from the Oxytricha nova sequence d(GGGGTTTTGGGG) are reported, in

28 heart-shaped structure of ciliated protozoan Oxytricha nova TEBPalpha-beta complex, POT1-TPP1 adopts

29 an TPP1, Schizosaccharomyces pombe Tpz1, and Oxytricha nova TEBPbeta.

30 The Oxytricha nova telemere binding protein alpha subunit bi

31 ations of the alpha and beta subunits of the Oxytricha nova telomere binding protein have been invest

32 Interactions of the beta subunit of the Oxytricha nova telomere binding protein with the telomer

33 ned the crystal structure of the two-subunit Oxytricha nova telomere end binding protein (OnTEBP) com

34 The Oxytricha nova telomere end binding protein (OnTEBP) rec

35 Oxytricha nova telomere end binding protein (OnTEBP) spe

36 terpoint to the interactions observed in the Oxytricha nova telomere end-binding and Schizosaccharomy

37 ferent non-cognate ssDNAs complexed with the Oxytricha nova telomere end-binding protein (OnTEBP) and

38 Oxytricha nova telomere end-binding protein specifically

39 Tl+ form of the G-quadruplex formed from the Oxytricha nova telomere sequence, d(G4T4G4), has been so

40 ucleotides with the repeat sequence found in Oxytricha nova telomeres.

41 The solution secondary structure of the Oxytricha nova telomeric 3' overhang, d(T4G4)2, has been

42 ic antiparallel G-quadruplex formed from the Oxytricha nova telomeric DNA sequence d(GGGGTTTTGGGG), a

43 -syn-anti-syn-anti pattern observed with the Oxytricha nova telomeric G-quadruplexes, have been well

44 ar model for the hairpin conformation of the Oxytricha nova telomeric repeat and consider its possibl

45 target a dimeric G-quadruplex formed by the Oxytricha nova telomeric sequence d(G(4)T(4)G(4)) with a

46 karyotes, and TEBP in the ciliated protozoan Oxytricha nova, exhibit sequence-specific binding to the

47 lymerase alpha gene, previously sequenced in Oxytricha nova, has been cloned from a genomic macronucl

48 sembled with telomere single-strand DNA from Oxytricha nova, our results highlight the relative simpl

49 In Oxytricha nova, telomere G-overhangs are protected by th

50 he telomere-binding protein alpha subunit of Oxytricha nova.

51 ymerase alpha (DNA pol alpha) in the ciliate Oxytricha nova.

52 f genomic DNA from Pneumocystis, Plasmodium, Oxytricha or Tetrahymena.

53 Oxytricha piRNAs map primarily to the somatic genome, re

54 BLASTO is accessible online at http://oxytricha.princeton.edu/BlastO.

55 to query the database is available at http://oxytricha.princeton.edu/dimorphism/database.htm.

56 free of charge and can be accessed at http://oxytricha.princeton.edu/GeneUnscrambler.htm

57 The SWAKK web server is available at http://oxytricha.princeton.edu/SWAKK/.

58 he current studies investigate the effect of Oxytricha single-stranded telomere DNA-binding protein s

59 with the duplex telomeric sequence from the Oxytricha species.

60 n orchestrate these genome rearrangements in Oxytricha, supporting an epigenetic model for sequence-d

61 ights into the solution conformations of the Oxytricha telomere binding protein subunits and serve as

62 me proteins, such as the beta-subunit of the Oxytricha telomere-binding protein, promote the formatio

63 -binding characteristics as the Euplotes and Oxytricha telomere-binding proteins.

64 A model for beta subunit binding by Oxytricha telomeric DNA sequences and a mechanism for qu

65 ther characterize structural polymorphism of Oxytricha telomeric DNAs, we have obtained and interpret

66 ick motifs, DNA refolding is specific to the Oxytricha telomeric hairpin and the retention of G.G pai

67 sence of the 5' thymidine tail preceding the Oxytricha telomeric repeat has no apparent effect on the

68 lts will be useful for probing structures of Oxytricha telomeric repeats in complexes with telomere e

69 In some ciliates including Oxytricha, the macronuclear genome is particularly extre

70 In Oxytricha, the somatic genome is responsible for vegetat

71 MAC chromosomes and their gene expression in Oxytricha trifallax (CI: Spirotrichea).

72 ncoding DNA polymerasealpha(DNA polalpha) in Oxytricha trifallax and compared it to the previously pu

73 or two completely sequenced ciliate genomes: Oxytricha trifallax and Tetrahymena thermophila.

74 earrangements in the single-celled eukaryote Oxytricha trifallax completely rewire its germline into

75 scribe a chimeric macronuclear chromosome in Oxytricha trifallax constructed from two smaller macronu

76 The somatic nucleus of Oxytricha trifallax contains over 15,000 different chrom

77 ing development of the somatic macronucleus, Oxytricha trifallax destroys 95% of its germ line, sever

78 exaggerated process of genome rearrangement, Oxytricha trifallax destroys 95% of its germline genome

79 The macronuclear genome of the ciliate Oxytricha trifallax displays an extreme and unique eukar

80 sity is approximately 4.0%), suggesting that Oxytricha trifallax may have one of the largest known ef

81 l a set of Piwi-interacting RNAs (piRNAs) in Oxytricha trifallax that likewise enable genomic self ve

82 unusual genomic organization of the ciliate Oxytricha trifallax to screen for eukaryotic non-coding

83 d into these three genes during evolution of Oxytricha trifallax, slightly modifying scrambling patte

84 BP alpha subunit have now been identified in Oxytricha trifallax, Stylonychia mytilis, Euplotes crass

85 otrichs, Stylonychia lemnae, S. mytilus, and Oxytricha trifallax, that independently derived the same

86 cloning and sequencing of the ortholog from Oxytricha trifallax.

87 e groups of ciliates, such as Stylonychia or Oxytricha, where extensive gene rearrangement occurs dur