ライフサイエンスコーパス: P. denitrificans

1 P. denitrificans is the first system described in which

2 For both bovine and P. denitrificans oxidase, the major features of the IR d

3 the sequence and structures of the human and P. denitrificans enzymes as models, a detailed sequence

4 g of ionic differences between the human and P. denitrificans ETF onto the structure identifies a sur

5 The X-ray crystal structures of human and P. denitrificans ETFs are very similar.

6 he electrostatic potentials of the human and P. denitrificans ETFs reveals that the P. denitrificans

7 gether with a functional interaction between P. denitrificans TIR and MyD88 visualized in a co-immuno

8 try of NQO1 through -6 of the membrane-bound P. denitrificans NDH-1 has been determined by radioimmun

9 NQO4, -5, and -6 subunits in membrane-bound P. denitrificans NDH-1 were extracted by treatment at al

10 the disproportionation reaction catalyzed by P. denitrificans electron transfer flavoprotein-ubiquino

11 nt X-ray structure reported for the complete P. denitrificans cytochrome aa3 molecule and the enginee

12 In the cholate-treated NDH-1-enriched P. denitrificans membranes, we observed EPR signals aris

13 trons from cytochromes c-551i and c-550 from P. denitrificans.

14 hable from those of the enzyme isolated from P. denitrificans.

15 Expression of active MADH from P. denitrificans requires four other genes in addition t

16 the CuA center of cytochrome c oxidase from P. denitrificans.

17 of a model of the previously described human-P. denitrificans chimeric ETF protein, it is possible to

18 ) in bovine oxidase (1542 and 1314 cm(-1) in P. denitrificans) and a positive band at approximately 1

19 lavin adenine dinucleotide (FAD) cofactor in P. denitrificans ETF gave two distributions of distances

20 RNA structural features previously found in P. denitrificans are present also in the 5S RNA of Rb. s

21 ped for the homologous expression of MauG in P. denitrificans.

22 ue interacts less strongly with the metal in P. denitrificans amicyanin than in Paracoccus versutus a

23 A DeltacbiX mutant in P. denitrificans was unable to respire anaerobically on

24 omplement deletions in both narK and nasA in P. denitrificans, suggesting that, while these proteins

25 ation have been found-an aerobic pathway (in P. denitrificans) and an anaerobic pathway (in P. sherma

26 ng alphaT244 has the same structural role in P. denitrificans ETF.

27 l model for nitrate and nitrite transport in P. denitrificans is proposed.

28 -sulfur, correspond to those of the isolated P. denitrificans NADH-dehydrogenase complex.

29 Notably, P. denitrificans emits approximately 40% of NO(3) (-) co

30 ion of the gamma subunit of the F1-ATPase of P. denitrificans by a hitherto unknown quaternary struct

31 Heterologous inhibition of the F1-ATPase of P. denitrificans by the mitochondrial IF1 supported both

32 the methylamine utilization gene cluster of P. denitrificans, mauFBEDACJG, were placed under the reg

33 Immunoprecipitation of labeled membranes of P. denitrificans and T. thermophilus established photoaf

34 The F1-ATPase and F1-zeta models of P. denitrificans were supported by cross-linking, limite

35 to explore the denitrification phenotypes of P. denitrificans and A. xylosoxidans at a range of extra

36 as calculated using the crystal structure of P. denitrificans ETF, which agrees with the major compon

37 methionine for T244 in the alpha subunit of P. denitrificans ETF and expressed the mutant ETF in Esc

38 cterial complex, we establish the utility of P. denitrificans complex I as a model system for the mam

39 on transfer reaction between either human or P. denitrificans ETF and MCAD demonstrates that the huma

40 synthetic bacteria, namely, Rb. sphaeroides, P. denitrificans, and Rhodospirillum rubrum.

41 It is proposed that P. denitrificans CcmG acts in vivo to reduce protein-dis

42 e in situ hybridization analyses showed that P. denitrificans was dominant (>50%) after 6 months of e

43 timated the total number of cofactors in the P. denitrificans NDH-1; the enzyme complex contains one

44 very similar to those of N1a cluster in the P. denitrificans NQO2 subunit.

45 This includes H456A, where in the P. denitrificans oxidase a leucine residue substituted f

46 ke the human structure, the structure of the P. denitrificans ETF is comprised of three distinct doma

47 y of the 7 subunits (NQO1-6 and NQO9) of the P. denitrificans NDH-1 in the membranes were investigate

48 ichiometry of the peripheral subunits of the P. denitrificans NDH-1 was completed by radioimmunologic

49 the NQO1 through -6 subunits per mol of the P. denitrificans NDH-1.

50 II is the equivalent of residue E78II of the P. denitrificans oxidase).

51 n and P. denitrificans ETFs reveals that the P. denitrificans ETF is more negatively charged.

52 , characterized, and compared with wild type P. denitrificans ETF.

53 proximately 10 mequiv) ionic strength, while P. denitrificans ETF is a better electron acceptor at hi