ライフサイエンスコーパス: P. mirabilis

1 P. mirabilis bacteriuria may lead to acute pyelonephriti

2 P. mirabilis HI4320 carrying the UreD-GFP fusion plasmid

3 P. mirabilis is capable of swarming, a form of multicell

4 P. mirabilis(pBAC001), which expresses green fluorescent

5 By performing primary screening of 2088 P. mirabilis transposon mutants, we identified 502 mutan

6 , of 683 E. coli, 371 K. pneumoniae, and 232 P. mirabilis isolates tested, 13 (1.9%), 28 (7.6%), and

7 On agar, a P. mirabilis colony grows outward in a bull's-eye patter

8 A cosmid clone, isolated from a P. mirabilis genomic library by hybridization with the 3

9 In a P. mirabilis speA mutant with greatly reduced levels of

10 were not observed during coinfection with a P. mirabilis urease mutant.

11 Screening for the presence of ICEPm1 among P. mirabilis colonizing isolates showed that ICEPm1 is m

12 To identify interactions among P. mirabilis accessory proteins, in vitro immunoprecipit

13 this study, microarrays were used to analyze P. mirabilis gene expression in vivo from experimentally

14 Fluorescent E. coli and P. mirabilis bacteria were observed by fluorescence micr

15 In particular, for both the E. coli and P. mirabilis orthologs, Lrp responsiveness to methionine

16 E. coli and P. mirabilis remained susceptible to most of the drugs t

17 y urea in both Escherichia coli DH5alpha and P. mirabilis HI4320.

18 onal start sites for the plasmid-encoded and P. mirabilis divergent promoters were similar in an Esch

19 ranscription at both the plasmid-encoded and P. mirabilis promoters.

20 inding sites in both the plasmid-encoded and P. mirabilis ureRp-ureDp intergenic regions.

21 tracellular survival of L. monocytogenes and P. mirabilis was assayed.

22 our institution, E. coli, K. pneumoniae, and P. mirabilis harbor plasmid-mediated AmpC enzymes.

23 s), Enterococcus faecalis (two strains), and P. mirabilis, E. coli (two strains), with statistically

24 imited zinc present in the urinary tract and P. mirabilis must scavenge this ion to colonize and pers

25 reased internalization of S. typhimurium and P. mirabilis by both HT-29 and Caco-2 enterocytes and wi

26 ined the epidemiology of catheter-associated P. mirabilis infections by use of pulsed-field gel elect

27 Our results establish a relationship between P. mirabilis flagellum density and cell motility in visc

28 While there are similarities between P. mirabilis MR/P and E. coli P fimbriae, there are more

29 pectedly and unlike other fliL mutants, both P. mirabilis and E. coli DeltafliL cells swarm (Swr(+)).

30 ing the formation of crystalline biofilms by P. mirabilis.

31 /P) fimbriae, a surface antigen expressed by P. mirabilis during experimental urinary tract infection

32 nsight into crystalline biofilm formation by P. mirabilis, including the link between biofilm formati

33 rotection against urinary tract infection by P. mirabilis (P < 0.002).

34 ce from ascending urinary tract infection by P. mirabilis (P < 0.05).

35 obial catheter colonization, particularly by P. mirabilis and other urease-positive bacteria.

36 A recombinant plasmid containing cloned P. mirabilis hns was able to complement and restore repr

37 entification of 37 consistently out-competed P. mirabilis transposon mutants, 25 of which were out-co

38 5) transconjugants/donor to ICEPm1-deficient P. mirabilis using plate mating assays with clinical iso

39 ability to sense a surface: e.g., DeltafliL P. mirabilis cells swarm precociously over surfaces with

40 form between swarming colonies of different P. mirabilis strains but not between colonies of a singl

41 ic of urease-mediated urea hydrolysis during P. mirabilis infection.

42 ormed significantly more biofilm than either P. mirabilis HI4320 (P = 0.03) or MR/P OFF (P = 0.05).

43 by this organism, was sufficient to enhance P. mirabilis urease activity and increase disease severi

44 Other uropathogens also enhanced P. mirabilis urease activity in vitro, including recent

45 In order for P. mirabilis to swarm, it first needs to detect a surfac

46 d key metabolic pathways as requirements for P. mirabilis infection of the urinary tract.

47 ine metabolism as an adaptation strategy for P. mirabilis and contributes to better understand the ec

48 rtance of siderophore production in vivo for P. mirabilis.

49 Modeling CaiT from P. mirabilis in the outward-open and closed states on th

50 How P. mirabilis senses a surface is not fully understood; h

51 Recent work has elucidated how P. mirabilis causes all of these disease states.

52 After 7 days, however, P. mirabilis HI4320 formed a 65-mum-thick biofilm, while

53 monia availability due to urease activity in P. mirabilis did not drive this gene expression.

54 Inactivation of aipA in P. mirabilis strains significantly (P < 0.01) reduced th

55 Although in P. mirabilis HI4320, ICEPm1 is annotated as integrated i

56 ty and antigenicity of an autotransporter in P. mirabilis and its use in vaccine development.

57 characterizing trimeric autotransporters in P. mirabilis as afimbrial surface adhesins and autoagglu

58 unclear how energetically costly changes in P. mirabilis cell morphology translate into an advantage

59 ve putrescine importer, was characterized in P. mirabilis.

60 ar details of self-nonself discrimination in P. mirabilis.

61 oxetine and thioridazine inhibited efflux in P. mirabilis, and molecular modelling predicted both dru

62 uoxetine and thioridazine) to act as EPIs in P. mirabilis, and control crystalline biofilm formation.

63 insertion was mapped to a speA homologue in P. mirabilis.

64 However, in P. mirabilis HI4320, transcription of ureR initiated pre

65 ellar basal body protein FliL is involved in P. mirabilis surface sensing.

66 Swimming and swarming motilies in P. mirabilis were also significantly reduced by both EPI

67 at the 3' end of the mrp fimbrial operon in P. mirabilis.

68 eus mirabilis; ectopic expression of papX in P. mirabilis reduces motility.

69 as an immunogenic outer membrane protein in P. mirabilis.

70 the first report of carbapenem resistance in P. mirabilis caused by the acquisition of bla(KPC).

71 This chromosome integration system in P. mirabilis provides an important tool for animal and b

72 veral related Phytophthora species including P. mirabilis, P. ipomoeae, and possibly P. phaseoli.

73 y tract, a ureR mutation was introduced into P. mirabilis HI4320 by homologous recombination.

74 Tn7 site-specific transposition pathway into P. mirabilis by transformation, followed by selection of

75 In the lab, P. mirabilis cells become long and multinucleate and inc

76 In liquid, P. mirabilis cells are 1.5- to 2.0-mum swimmer cells wit

77 ded that MR/P fimbriae are expressed by most P. mirabilis cells infecting the urinary tract, dictate

78 a contribute significantly to the ability of P. mirabilis to colonize the urinary tract and cause acu

79 us, ids genes are involved in the ability of P. mirabilis to distinguish self from nonself.

80 Microarray analysis of P. mirabilis HI4320 cultured under iron limitation ident

81 ity, the AT also promoted autoaggregation of P. mirabilis and this function was independent of its pr

82 The crystalline biofilms of P. mirabilis can cause serious complications for patient

83 the flagellar operon, in vegetative cells of P. mirabilis and found that increased flagellum density

84 challenged transurethrally with 10(7) CFU of P. mirabilis BA6163 (wild type) (n = 16), WPM111 (hpmA m

85 rom infection (mean log(10) number of CFU of P. mirabilis Nal(r) HI4320 per milliliter or gram in vac

86 he initial epidemiologic characterization of P. mirabilis isolates.

87 (encoding the entire urease gene cluster of P. mirabilis) was equivalent in both the H-NS(-) backgro

88 may be a significant virulence component of P. mirabilis in urinary tract infections.

89 eye colony often associated with cultures of P. mirabilis.

90 n identified as important for development of P. mirabilis crystalline biofilms, highlighting the pote

91 e often invasive, and, with the exception of P. mirabilis, are multiclonal.

92 nces swarming-associated colony expansion of P. mirabilis under anaerobic conditions on a solid surfa

93 To investigate in vivo expression of P. mirabilis urease, the gene encoding green fluorescent

94 pproach to investigate in vivo expression of P. mirabilis virulence genes in experimental urinary tra

95 Ambient-temperature fimbriae of P. mirabilis may represent a novel type of fimbriae of e

96 patients revealed that a single genotype of P. mirabilis can persist in the urinary tract despite ma

97 Transurethral inoculation of P. mirabilis(pBAC001) resulted in ascending urinary trac

98 mrpI null mutants from a clinical isolate of P. mirabilis, HI4320.

99 Examination of a set of 55 isolates of P. mirabilis, each from a different clinical or environm

100 Immunogold electron microscopy of P. mirabilis HI4320 revealed that MrpH was located at th

101 ated autoagglutination, and a taaP mutant of P. mirabilis showed significantly (P < 0.05) more reduce

102 H5alpha and an isogenic mrpH::aphA mutant of P. mirabilis were unable to produce normal MR/P fimbriae

103 otype on an otherwise nonhemolytic mutant of P. mirabilis.

104 icantly reduced in an isogenic pta mutant of P. mirabilis.

105 ucted and characterized DeltafliL mutants of P. mirabilis and Escherichia coli.

106 I to evaluate the colonization of mutants of P. mirabilis HI4320 that were generated by signature-tag

107 The number of P. mirabilis cells adhering to bladder tissue did not ap

108 ociated and important for the persistence of P. mirabilis in the host, it was selected as a vaccine c

109 y contributes to the pathogenic potential of P. mirabilis in the urinary tract.

110 microscopy, we demonstrated the presence of P. mirabilis within the urease-induced stone matrix.

111 inhibitors in the treatment or prevention of P. mirabilis crystalline biofilms.

112 so found to significantly reduce the rate of P. mirabilis crystalline biofilm formation on catheters,

113 port a role of MrpJ as a global regulator of P. mirabilis virulence.

114 re we report the complete genome sequence of P. mirabilis HI4320, a representative strain cultured in

115 with the newly completed genome sequence of P. mirabilis HI4320, was used to identify surface-expose

116 Strains of P. mirabilis with mutations in three of the correspondin

117 ethods for identifying individual strains of P. mirabilis.

118 ng deposits in bladder and kidney tissues of P. mirabilis-infected mice.

119 also contributes to the uropathogenicity of P. mirabilis.

120 tion of urease by urea, and for virulence of P. mirabilis in the urinary tract.

121 eted into the culture medium by the original P. mirabilis flgN mutant demonstrated that export of Flg

122 Overall, P. mirabilis exhibits an extraordinary lifestyle, and fu

123 odes a 135-amino acid residue protein, PMTR (P. mirabilis transcription regulator), a new member of t

124 One of seven positive P. mirabilis isolates was in group II, with the remainde

125 Five of seven P. mirabilis isolates were from blood cultures.

126 the CTX-M-positive isolates showed that six P. mirabilis isolates were clonal and that there were se

127 We conclude that P. mirabilis and P. stuartii coinfection promotes urolit

128 These results support the hypothesis that P. mirabilis ascertains its location in the environment

129 Further analysis revealed that P. mirabilis DeltafliL cells also exhibit an alteration

130 In this study, we show that P. mirabilis CaUTI isolates initiate swarming in respons

131 -feeding and biochemical analysis shows that P. mirabilis is unable to utilize or produce yersiniabac

132 annot utilize citrate, the data suggest that P. mirabilis uses glutamate dehydrogenase to monitor car

133 In addition, the P. mirabilis HI4320 genome possesses four tandem copies

134 sbA (regulator of swarming behavior) and the P. mirabilis homologs to RcsB and RcsC.

135 Sequencing of the P. mirabilis genome revealed 14 additional paralogues of

136 These data underscore the importance of the P. mirabilis IgA-degrading metalloprotease in UTI.

137 und to be localized to a 5.4-kb locus on the P. mirabilis genome encoding RsbA (regulator of swarming

138 The results presented here suggest that the P. mirabilis and plasmid-encoded urease gene clusters ut

139 In this report, we demonstrate that the P. mirabilis urease gene cluster contains similar diverg

140 We show that the P. mirabilis, Vibrio harveyi, and E. coli Crl homologs f

141 Thus, P. mirabilis appears to use a related mechanism to inhib

142 and TaaP individually offered advantages to P. mirabilis in a murine model.

143 more prevalent in urine isolates compared to P. mirabilis strains isolated from other body sites (P<0

144 The majority of wild-type P. mirabilis cells in transurethrally infected mice prod

145 Wild-type P. mirabilis was usually found colonizing intact uroepit

146 In wild-type P. mirabilis, increased expression of the flhDC operon o

147 ncatheterized mice to infection by wild-type P. mirabilis.

148 bladder colonization factor of uropathogenic P. mirabilis and also suggested that the ability to swit

149 This report suggests that in vivo, P. mirabilis UreD may be important for recruitment of Ur

150 When P. mirabilis encounters a highly viscous environment, e.

151 When P. mirabilis encounters a solid surface, where flagellar

152 on and motility, a result also observed when P. mirabilis fliL+ was expressed in Escherichia coli.

153 the consolidation phase is a state in which P. mirabilis prepares for the next wave of swarming.

154 ull's-eye colonies typically associated with P. mirabilis.

155 reviously demonstrated that coinfection with P. mirabilis and P. stuartii increased overall urease ac

156 Furthermore, colonization with P. mirabilis promoted intestinal inflammation upon intes

157 Mice were infected with P. mirabilis or a urease mutant, P. stuartii, or a combi

158 neys from mice transurethrally infected with P. mirabilis were used to prepare template DNA for PCR a

159 sera from mice experimentally infected with P. mirabilis.

160 We conclude that prior infection with P. mirabilis does not protect significantly against homo