ライフサイエンスコーパス: P. putida

1 P. putida arsH expressed in E. coli conferred resistance

2 P. putida expressing the cloned protease IV gene had sig

3 P. putida F1 also responded weakly to cytidine, uridine,

4 P. putida JS110 and JS112, mutant strains which do not e

5 P. putida KT2440 catabolized the d-stereoisomers of lysi

6 P. putida producing protease IV, relative to P. putida w

7 A P. putida G7 nahY mutant grew on naphthalene but was not

8 A P. putida pcaK mutant was defective in its ability to ac

9 BkdR levels were elevated in a P. putida crc mutant, but bkdR transcript levels were th

10 in multiple copies, into the chromosome of a P. putida recipient.

11 the isolates in the P. fluorescens (57) and P. putida (19) groups.

12 However, Pseudomonas aeruginosa and P. putida biofilms remained insensitive to CCCP addition

13 hing expression systems in P. aeruginosa and P. putida, and 'GFP-tagging' Y. pestis.

14 orthologues from Pseudomonas aeruginosa and P. putida, we have determined that these operons encode

15 mined E1beta structures from humans (HU) and P. putida (PP).

16 yl site concentrations for S. oneidensis and P. putida samples when grown in the TSB medium.

17 ter sp. strain ADP1, P. aeruginosa PAO1, and P. putida P111.

18 ved among environmental pseudomonads such as P. putida KT2440, P. fluorescens PfO1 and P. fluorescens

19 fprA expression in both P. putida and P. aeruginosa was found to be regulated by

20 essary for effective sulfate assimilation by P. putida and that the effect of finR mutation on PDTC p

21 ion by higher PEf1 propagation was offset by P. putida lysis, which decreased stress from interspecie

22 ecreased survival of desiccation not only by P. putida but also by Pseudomonas aeruginosa PAO1 and Ps

23 are located on the EPS molecules produced by P. putida.

24 , each of these amino acids was racemized by P. putida KT2440 enzymes.

25 ) show that nano-Se particles synthesized by P. putida have a size range of 100 to 500 nm and that th

26 ts on the physiology and survival of certain P. putida strains throughout their natural history.

27 logous to sequences present in the completed P. putida KT2440 genome sequence or plasmid pWWO sequenc

28 In contrast, P. putida Idaho demonstrated an increase in phospholipid

29 of the iron-sulfur clusters in the different P. putida FNR proteins influence their reactivity with O

30 Transcriptome analysis of DIMBOA-exposed P. putida identified increased transcription of genes co

31 Following o-xylene exposure, P. putida MW1200 exhibited a decrease in total phospholi

32 h Green Fluorescent Protein (GFP)-expressing P. putida showed that DIMBOA-producing roots of wild-typ

33 s of genomic information for P. fluorescens, P. putida, and P. stutzeri suggests that the findings re

34 al cells detached from biofilm (over 70% for P. putida and approximately 40% for polysaccharide produ

35 ow FFF and MALDI-TOF MS was demonstrated for P. putida and E. coli.

36 A transport, and they were also required for P. putida to have a chemotactic response to 4-HBA.

37 s that do not serve as growth substrates for P. putida F1.

38 inellus sp. (5.1 x 10(-2) mM d(-1)) than for P. putida (2.32 mM d(-1)).

39 slightly motile, stationary-phase cells from P. putida G7 were mobilized effectively, but the activel

40 The structure of a QuiC1 enzyme from P. putida reveals that the protein is a fusion of two di

41 early biofilm formation, HSLs extracted from P. putida and pure C(12)-HSL were added to 6-h planktoni

42 he octapeptide in case of pseudobactins from P. putida ATCC 39167 is Chr-Ser(1)-Ala(1)-AcOHOrn-Gly-Al

43 unlike PchF obtained from PCMH purified from P. putida, FAD was bound noncovalently.

44 d for the homologous mandelate racemase from P. putida, also a member of the enolase superfamily whos

45 of Ppmt-2 sigma70 with that of sigma70 from P. putida strain G1 shows that the two proteins differ i

46 l subunits (Mr = 142,000), whereas that from P. putida is composed of two functionally different prot

47 The N-DBP yield from P. putida EPS was two times higher than that of P. aerug

48 Functional P. putida and P. aeruginosa transporters were identified

49 GE P. putida showed high arsenic methylation and volatiliza

50 le expression of an arsM-gfp fusion gene (GE P. putida), which was inserted into the bacterial chromo

51 that was administered to the patients, grew P. putida with a pulsed-field gel electrophoresis (PFGE)

52 In P. putida, all these genes (with the exception of pcaH,G

53 ed as the source of the ion near m/z 7684 in P. putida.

54 higher concentrations by reproducing also in P. putida (7.2 +/- 0.4 vs 6.0 +/- 1.0 log10PFU/mL).

55 sferable Hg(R) captured to the chromosome in P. putida A simple mathematical model suggests these dif

56 Homologous enzymes in P. putida and A. tumefaciens were identified based on a

57 X: free linolenic acid accumulated faster in P. putida BTP1-treated plants than in control.

58 lso observed to occur at high frequencies in P. putida PaW340.

59 nt arrangement of cym, cmt, and tod genes in P. putida F1.

60 ate of turnover was significantly greater in P. putida Idaho than in P. putida MW1200.

61 f the known stress tolerance traits known in P. putida but also recognizes the capacity of this bacte

62 aromatic compounds are intimately linked in P. putida.

63 am of the tod (toluene catabolism) operon in P. putida F1.

64 in the same orientation as the cmt operon in P. putida F1.

65 P. fluorescens allowed pQBR57 to persist in P. putida via source-sink transfer dynamics.

66 have a role in polar flagellar placement in P. putida.

67 use polyvalence enhanced PEf1 propagation in P. putida.

68 The reaction with proteins in P. putida EPS multiplied both the time and the monochlor

69 formation regulation by the Rsm proteins in P. putida.

70 BR57 by nontransferable chromosomal Hg(R) in P. putida was slowed in coculture.

71 57's lower intraspecific conjugation rate in P. putida By contrast, in two-species communities, both

72 romoter with a consensus FNR-binding site in P. putida and E. coli strains expressing only one FNR pr

73 we revealed the catabolic pathway for SQ in P. putida SQ1 through differential proteomics and transc

74 ificantly greater in P. putida Idaho than in P. putida MW1200.

75 Ppu12 derivative introduced exogenously into P. putida PP3 via the suicide donor pAWT50 resulted in s

76 s C, plasmid concentration of 0.8 ng/microl, P. putida UWC1 cell concentration of 2.5 x 10(9) CFU (co

77 Finally, benzoate represses the ability of P. putida to transport 4-hydroxybenzoate (4-HBA) by prev

78 catalase probe, generated by PCR analysis of P. putida genomic DNA with oligomers based on typical ca

79 ly expressed following initial attachment of P. putida.

80 ctively motile, exponentially grown cells of P. putida G7 were not mobilized.

81 ry, but not in exponentially grown, cells of P. putida G7.

82 As a proof of principle, induced cultures of P. putida KT2440 producing an EGFP-fused model protein b

83 HSL were added to 6-h planktonic cultures of P. putida, and cell extracts were analyzed by 2-D gel pr

84 A derivative of P. putida with both arsH genes deleted is sensitive to M

85 on, recovers the mean-square displacement of P. putida if the two distinct swimming speeds are taken

86 that the bimodal turn angle distribution of P. putida reduced collisions with obstacles in porous me

87 on yields pyruvate, which supports growth of P. putida, and 3-sulfolactaldehyde (SLA), which is oxidi

88 ptake processes in the genome-scale model of P. putida.

89 Chemotaxis assays confirmed motility of P. putida towards DIMBOA.

90 Chromosomal mutants of P. putida deficient in catalase C, obtained by gene inte

91 aize attract significantly higher numbers of P. putida cells than roots of the DIMBOA-deficient bx1 m

92 regulates the plasmid-borne pheBA operon of P. putida PaW85, which is involved in phenol catabolism.

93 The bkd operons of P. putida and P. aeruginosa encode the inducible multien

94 We investigated the in vitro persistence of P. putida in heparinized saline: even under refrigerated

95 1 g/L nZVI induced a persistent phenotype of P. putida F1 as indicated by smaller colony morphology,

96 The annotated proline racemase ProR of P. putida KT2440 showed negligible activity with either

97 Microbial growth rates of P. putida in subsurface environments can only be accurat

98 A screen of P. putida F1 mutants, each lacking one of the genes enco

99 ficant increase in the copper sensitivity of P. putida KT2440 under the conditions tested.

100 revealed that the default metabolic state of P. putida KT2440 is characterized by a slight catabolic

101 of different samples with respect to that of P. putida.

102 of nano-Se and the metabolic versatility of P. putida offer the opportunity to use this model organi

103 .e., the strain was either P. fluorescens or P. putida, but the system did not make the distinction a

104 Single inoculations with R. irregularis or P. putida had differential growth effects on both cultiv

105 r single inoculations with R. irregularis or P. putida, only the cultivar with high mycorrhizal compa

106 gene clusters were found in genomes of other P. putida strains, in other gamma-Proteobacteria, and in

107 We show here that recombinant P. putida glyoxalase I is an active dimer (kcat approxim

108 Analysis of phospholipid biosynthesis showed P. putida Idaho to have a higher basal rate of phospholi

109 s with more competing soil bacteria species, P. putida lysis was less critical in mitigating interspe

110 a protease IV-negative Pseudomonas species, P. putida.

111 utida Idaho, and a solvent-sensitive strain, P. putida MW1200, were examined in terms of phospholipid

112 The responses of a solvent-tolerant strain, P. putida Idaho, and a solvent-sensitive strain, P. puti

113 Like other prokaryotic swimmers, P. putida exhibits a motion pattern dominated by persist

114 The results presented here demonstrate that P. putida undergoes a global change in gene expression f

115 A transport and chemotaxis demonstrates that P. putida has a chemoreceptor that differs from the clas

116 Our results show that P. putida is able to reduce selenite aerobically, but no

117 These results suggest that P. putida Idaho has a greater ability than the solvent-s

118 The P. putida enzyme had a Km for NG of 52 +/- 4 microM, a K

119 The P. putida gene, xenA, encodes a 39,702-Da monomeric, NAD

120 A 13-kb cloned DNA fragment containing the P. putida crc gene region was sequenced.

121 We genetically engineered the P. putida KT2440 with stable expression of an arsM-gfp f

122 when it expressed the five enzymes from the P. putida operon.

123 ayer between bulk and biofilm surface in the P. putida biofilm compared to those of P. aeruginosa bio

124 Like the P. putida enzyme, hh4-OT requires the amino-terminal pro

125 her persistence in the middle section of the P. putida biofilm compared to the P. aeruginosa biofilms

126 ics by numerical integration showed that the P. putida enzyme produced an approximately 2-fold molar

127 ruginosa helicase and significantly with the P. putida helicase, whereas deletion of amino acids 71-8

128 ced activity in vitro, particularly with the P. putida helicase.

129 loped an adenoviral vector inserted with the P. putida methioninase (MET) gene (rAd-MET).

130 BLAST analyses conducted with the P. putida xenA and the P. fluorescens xenB sequences dem

131 These P. putida cell extracts produced a protein with the same

132 P. putida producing protease IV, relative to P. putida with the vector alone, caused a threefold incr

133 mass yield of the evolved D-xylose utilizing P. putida strain.

134 nstrated that these enzymes are induced when P. putida is grown in the presence of 3-chlorobenzoate,

135 Fractionation obtained with P. putida GPo1 was similar to acid hydrolysis and M. aus

136 emoval, the measured sulfhydryl sites within P. putida samples was 34.9 +/- 9.5 mumol/g, and no sulfh