ライフサイエンスコーパス: P2Y(2) receptor

1 and ATP but not by diphosphate nucleotides (P2Y2 receptor).

2 ctivated Cl- conductance is regulated by the P2Y2 receptor.

3 that is pharmacologically distinct from the P2Y2 receptor.

4 released ATP, which activates the purinergic P2Y2 receptor.

5 play a major role in apical targeting of the P2Y2 receptor.

6 ere similar for the wild-type and all mutant P2Y2 receptors.

7 uding UTP, decrease ENaC activity via apical P2Y2 receptors.

8 shape, confirming the presence of functional P2Y2 receptors.

9 ective antagonist, suggesting a role for the P2Y(2) receptor.

10 hasizing the essential roles for ATP and the P2Y(2) receptor.

11 ted inositol phosphate response of the M1 or P2Y(2) receptor.

12 expression, indicating a mitogenic role for P2Y(2) receptors.

13 acids with the highest homology to the human P2Y(2) receptor (84%).

14 enetic or small interfering RNA depletion of P2Y(2) receptors abolished the ATPgammaS-mediated increa

15 In addition, P2Y(2) receptor activated ERK1/2, JNK, and p38 MAPK path

16 ade of the PLC/Ca(2+) pathway did not affect P2Y(2) receptor activation of p38, JNK, and TF induction

17 P2Y2 receptor activation by nucleotides thus provides ph

18 irway epithelial surface reach the range for P2Y2 receptor activation by physiological stimuli and id

19 criptase polymerase chain reaction) and that P2Y2 receptor activation is the principal means by which

20 The P2Y(2) receptor agonist INS37217 improves the rate of re

21 In contrast, the P2Y(2) receptor agonist UTP caused negligible ATP releas

22 1321N1 astrocytoma cells stimulated with the P2Y(2) receptor agonist UTP.

23 s show that uridine 5'-triphosphate (UTP), a P2Y(2) receptor agonist, is effective at acutely enhanci

24 n in A431 cell number (p<0.001), whereas the P2Y2 receptor agonist uridine 5'-triphosphate caused a s

25 the nonspecific P2 receptor agonist ATP, the P2Y2 receptor agonist UTP (p<0.001), and the P2Y1 recept

26 on of P2Y2-HA receptor-expressing cells with P2Y2 receptor agonists also resulted in receptor-specifi

27 ay goblet cells secrete mucin in response to P2Y2 receptor agonists and to secretagogues, phorbol 12-

28 Incubation of cells with P2Y2 receptor agonists resulted in a concentration of ag

29 Results indicated that the equipotent P2Y2 receptor agonists UTP or ATP (1-100 microm) stimula

30 its the EGFR into a protein complex with the P2Y(2) receptor and allows Src to efficiently phosphoryl

31 Dual immunofluorescence labeling of the P2Y(2) receptor and the EGFR indicated that UTP caused a

32 ssociation of tyrosine receptor kinase A and P2Y(2) receptors and is required for enhanced neuronal d

33 tinct temporal profiles of PLC activation by P2Y(2) receptors and mAChR may arise from the differenti

34 ne further the signaling cascade between the P2Y2 receptor and MAP kinase.

35 tor subtypes, the Gq-coupled ATP/UTP-sensing P2Y2 receptor and the Gs-coupled A2b adenosine receptor.

36 Wild-type P2Y2 receptors and a series of five C-terminal truncatio

37 was equipotent to UTP as an agonist at human P2Y2 receptors and also activated P2Y4 receptors with an

38 teracting systems--extracellular nucleotides/P2Y2 receptors and neurotrophin/TrkA--to sustain neurona

39 UTP activates P2Y2 receptors and signals only through Gq/11.

40 protein kinase responses through endogenous P2Y2 receptors, and these responses were not blocked by

41 2, and P2Y13 receptors; the ATP/UTP-specific P2Y2 receptor; and the ATP-selective P2Y11 receptor.

42 dder neuron excitability were blocked by the P2Y(2) receptor antagonist suramin.

43 cade immediately upstream of MAP kinase, the P2Y2 receptor appears to uniquely utilize [Ca2+]i, PKC,

44 lease and autocrine purinergic signaling via P2Y2 receptors are essential for PMN activation.

45 2X7 or P2Y2 receptor knock-out mice indicate P2Y2 receptors are involved in the activation of STAT3 a

46 , UTP, and ATPgammaS, an effect mediated via P2Y2 receptors, as demonstrated by small interfering RNA

47 fluid "pump" function in vitro by activating P2Y(2) receptors at the apical membrane.

48 lease and autocrine purinergic signaling via P2Y2 receptors at the front and A2a receptors at the bac

49 , which indicates upregulation of functional P2Y2 receptors at which UTP and ATP are equipotent agoni

50 nhibited by suramin or by siRNA silencing of P2Y(2) receptor, but not by NF-157, a P2Y(11)-selective

51 only by basolateral nucleotides, mediated by P2Y(2) receptors, but also by luminal nucleotides, media

52 Activation of epithelial P2Y(2) receptors by UTP generated two patterns of [Ca(2+

53 ted that the activation of G-protein-coupled P2Y2 receptors by extracellular ATP and UTP stimulated M

54 addressed the hypothesis that activation of P2Y2 receptors by extracellular nucleotides modulates th

55 Activation of P2Y(2) receptors causes Galpha(q/11) to associate with P

56 PANX1) channels and autocrine stimulation of P2Y2 receptors contribute to the excitatory signals at t

57 l roles in the human epidermis with P2Y1 and P2Y2 receptors controlling proliferation, while P2X5 and

58 , whereas inhibition by UTP, which activates P2Y2 receptors coupled to Gq/11 and Gi3, was not affecte

59 edullary thick ascending limbs isolated from P2Y2 receptor-deficient mice compared with wild type.

60 wing HlyA-induced IL-6 release was absent in P2Y2 receptor-deficient mice.

61 Molecular determinants of P2Y2 receptor desensitization and sequestration have bee

62 9-null endothelial cells was associated with P2Y2 receptor desensitization.

63 xycorticosterone acetate and deletion of the P2Y(2) receptor each modestly increased the resistance o

64 ine and adenine nucleotides with that of the P2Y2 receptor expressed in the same cells.

65 knockout mice, we propose that a blunting in P2Y2 receptor expression or activity is a new mechanism

66 nding of Src to the SH3 binding sites in the P2Y(2) receptor facilitates Src activation, which recrui

67 kinase C activation, which desensitizes the P2Y2 receptor, had no effect on sequestration, consisten

68 uent activation of Gq/G11-coupled purinergic P2Y2 receptors have been shown to mediate fluid shear st

69 that gene-targeted (knockout) mice that lack P2Y2 receptors have salt-resistant arterial hypertension

70 Furthermore, Src co-precipitated with the P2Y(2) receptor in 1321N1 astrocytoma cells stimulated w

71 These results support involvement of P2Y(2) receptors in bladder sensation, suggesting an imp

72 Genetic deletion of P2Y(2) receptors in mice (P2Y(2)(-/-); littermates to wi

73 Apical release of ATP and UTP can activate P2Y(2) receptors in the aldosterone-sensitive distal nep

74 c acid (DIDS), which has been shown to block P2Y(2) receptors in the RPE.

75 ndicating the far-reaching importance of the P2Y2 receptor in mediating migration.

76 of mRNA expression for the G protein-coupled P2Y2 receptor in mouse thymocytes after the addition of

77 pes indicates the predominant involvement of P2Y2 receptor in P2X3 inhibition, and immunolocalization

78 These findings suggest a novel role for the P2Y2 receptor in the p38- and Rho kinase-dependent expre

79 tracellular nucleotide signaling through the P2Y2 receptor in the survival of neurons: PC12 (pheochro

80 identify a previously unrecognized role for P2Y2 receptors in blood pressure regulation that is link

81 ar ATP is mediated through the activation of P2Y2 receptors in hepatocytes.

82 ular injury, suggesting a potential role for P2Y2 receptors in monocyte recruitment by vascular endot

83 hat mitochondria deliver ATP that stimulates P2Y2 receptors in response to chemotactic cues, and that

84 Loss of collecting duct P2Y2 receptors in the knock-out mouse is the primary def

85 The pattern of responses implied that P2Y2 receptors in visceral, and probably vascular, smoot

86 logy model of the nucleotide-activated human P2Y2 receptor, including loops, termini, and phospholipi

87 ATP stimulation of P2Y(2) receptors increased alpha-smooth muscle actin (al

88 ype is not yet known, but stimulation of the P2Y2 receptor increases IL-1-mediated PGE2 release.

89 These results suggest that the P2Y2 receptor-initiated activation of MAP kinase was dep

90 We conclude that the P2Y(2) receptor is the dominant P2Y purinoceptor that re

91 The data indicate that the P2Y2 receptor is activated with similar potencies by ATP

92 The targeting signal of the P2Y2 receptor is located between the N terminus and 7TM,

93 ATP release from renal epithelia, which via P2Y2 receptors is the main mediator of HlyA-induced [Ca(

94 ion that the G protein-coupled P2U receptor (P2Y2 receptor) is activated by UTP as well as ATP provid

95 oupled P2Y receptor that, like the mammalian P2Y2 receptor, is activated by both adenine and uridine

96 Similar studies performed on P2X7 or P2Y2 receptor knock-out mice indicate P2Y2 receptors are

97 prepared cardiomyocytes from B2 receptor or P2Y2 receptor knock-out mice, respectively.

98 by 5-8 fold, an effect largely eliminated by P2Y(2) receptor knockdown or ATP hydrolysis with apyrase

99 A mutant P2Y(2) receptor lacking the PXXP motifs was found to sti

100 These results suggest that P2Y2 receptors mediate the effects of purinoceptor agoni

101 dicate that the EGF receptor is required for P2Y2 receptor-mediated MAP kinase activation.

102 These results demonstrate that the P2Y2 receptor-mediated transactivation of the EGF recept

103 These findings demonstrate that P2Y(2) receptor mediates TF expression in HCAEC through

104 d contributes to hypertension that occurs in P2Y2 receptor-/- mice.

105 lymerase chain reaction analysis showed that P2Y(2) receptor mRNA was dramatically increased in cells

106 Cellular expression of P2Y(2) receptor mRNA was localized by nonradioisotopic i

107 P2Y(2) receptor mRNA was observed throughout the RPE and

108 It is proposed that the rapid increase of P2Y2 receptor mRNA expression could be a common early ev

109 After wounding, the P2Y2 receptor mRNA expression increased, and after knock

110 mocytes, the observed rapid up-regulation of P2Y2 receptor mRNA expression may reflect an immediate e

111 e possibility that the observed increases in P2Y2 receptor mRNA expression were due to the enrichment

112 ollar placement caused rapid upregulation of P2Y2 receptor mRNA in medial SMCs before appearance of n

113 P2Y2 receptor mRNA is upregulated in intimal lesions of

114 Although UDP does not activate the human P2Y2 receptor, mucosal addition of UDP promoted [3H]inos

115 P2Y2 receptor mutants were localized in a similar manner

116 At recombinant human P2Y1 and P2Y2 receptors, (N)-methanocarba-ATP was 138- and 41-fol

117 t PLC-beta1, is induced by activating either P2Y(2) receptors or M(3) mAChR.

118 To test for the role of the P2Y(2) receptor (P2Y(2)-R) in the regulation of nucleoti

119 epithelia, we tested the hypothesis that the P2Y(2) receptor (P2Y(2)-R) subtype mediates these respon

120 The G-protein-coupled P2Y2 receptor (P2Y2-R) is abundantly expressed on the ai

121 We recently reported that the P2Y2 receptor (P2Y2R) is the predominant nucleotide rece

122 ted that activation of the G protein-coupled P2Y2 receptor (P2Y2R) subtype expressed in human 1321N1

123 currents was mediated by a G-protein-coupled P2Y(2) receptor/PKC pathway, whereas the effect of UTP o

124 m the C-terminal domains of human P2Y(1) and P2Y(2) receptors produced a shift in the voltage depende

125 rs in response to chemotactic cues, and that P2Y2 receptors promote mTOR signaling, which augments mi

126 s a reporter system for detection of ATP via P2Y2 receptor-promoted formation of [3H]inositol phospha

127 Agonist occupation of the P2Y2 receptor promotes inhibition of Na+ absorption as w

128 (most likely, endothelial ATP/UTP-selective P2Y(2)) receptors, rather than via its dephosphorylation

129 This detailed view of P2Y2 receptor recognition suggests mutations for model v

130 In addition, silencing P2Y2 receptors reduced the level of P2Y2 mRNA by 75% and

131 identify the apical targeting signal in the P2Y2 receptor, regions of the P2Y2 receptor were progres

132 S-2 and primary osteoblasts express P2Y1 and P2Y2 receptors, respectively.

133 domains of either the human P2Y(1) or human P2Y(2) receptor resulted in voltage dependence and inact

134 ect on the capacity of nucleotides to induce P2Y2 receptor-specific desensitization.

135 urkey erythrocyte P2Y(1) or human P2Y(1) and P2Y(2) receptors stably expressed in astrocytoma cells.

136 lls, an airway goblet cell model, purinergic P2Y(2) receptor-stimulated increase of cytosolic Ca(2+)

137 helial cells was found to involve purinergic P2Y(2) receptor stimulation, and both ligand-dependent m

138 ated the expression of P2X5, P2X7, P2Y1, and P2Y2 receptor subtypes in 8- to 11-wk-old human fetal ep

139 gated the expression of P2X5, P2X7, P2Y1 and P2Y2 receptor subtypes in normal human epidermis and in

140 es indicate a key role of the apical ATP/UTP-P2Y(2)-receptor system in the inhibition of ENaC P(o) in

141 Mutation of RGD to RGE had no effect on P2Y2 receptor targeting, indicating that receptor-integr

142 tion to confirming the presence of classical P2Y2 receptors, these results also provide functional ev

143 For one class of P2Y receptors, P2Y2 receptors, this stimulation of adenylyl cyclase and

144 Thus, ATP release activates P2Y(2) receptors to mediate profibrotic responses in CFs

145 These results illustrate that the P2Y2 receptor undergoes agonist-promoted movement to an

146 P2Y2 receptor up-regulation and activation induces intim

147 The Gq/phospholipase C-linked human P2Y2 receptor was tagged at its amino terminus with the

148 Agonist-induced internalization of P2Y2 receptors was demonstrated directly by prelabeling

149 ibition by ATP, which activates both P2X and P2Y2 receptors, was not affected by zero Ca2+ alone; but

150 signal in the P2Y2 receptor, regions of the P2Y2 receptor were progressively substituted with the co

151 th, 1321N1 cells stably expressing the human P2Y2 receptor were used as a reporter system for detecti

152 P2Y2 receptors were found only in periderm cells and may

153 P2X5 and P2Y2 receptors were heavily expressed in basal cell carc

154 ell transfectants expressing the recombinant P2Y2 receptor, whereas vector-transfected control cells

155 In conclusion, we have cloned the porcine P2Y(2) receptor with novel pharmacology and demonstrated