ライフサイエンスコーパス: P450scc

1 BsAg) and membrane-bound cytochrome P450scc (P450scc).

2 cytochrome P450 side chain cleavage enzyme (P450scc).

3 bers of the P450 side chain cleavage system (P450scc).

4 protein, and side-chain cleavage activity of P450scc.

5 ndrial transfer of cholesterol to cytochrome P450scc.

6 the Fe2+-sensitive process does not involve P450scc.

7 ee Leydig cell steroid biosynthetic enzymes (p450scc, 3betaHSD and p450c17) were not expressed in XY

8 Presence of P450scc, adrenodoxin reductase, cytochrome P450 17-hydro

9 sponsive region (IGFRE) of porcine P450 11A (P450scc) after transfection into MCF-7 cells.

10 The mRNA for cytochrome P450scc and 3beta-HSD were detected only in actively mye

11 The mRNA for cytochrome P450scc and 3beta-hydroxysteroid dehydrogenase also were

12 Biochemical activity of cytochrome P450scc and P450c17 was demonstrated in SEB-1 sebocytes

13 tor, P2, that bind to a known IGFRE (porcine P450scc) and induce reporter gene transcriptional activi

14 ifferent molecular weights, such as BSA, Mb, P450scc, anti-HBsAg with the concentration detection lim

15 bserved elevations of cholesterol-cytochrome P450scc association and metabolism in subsequently isola

16 n-like growth factor 1 (IGF1)-inducible gene P450scc by binding to an insulin-like growth factor resp

17 It has been shown that mammalian cytochrome P450scc can metabolize vitamin D3 to 20-hydroxyvitamin D

18 that the cryoreduced ternary complex of oxy-P450scc-CH is catalytically competent and hydroxylates c

19 als that the distal pocket of the parent oxy-P450scc-cholesterol complex exhibits an efficient proton

20 uent annealing of the ternary oxy-cytochrome P450scc-cholesterol complex.

21 Even though the stingray P450scc contains an amino terminus longer than the other

22 tion of the expression of Cyp17, and perhaps P450scc, contributes to that effect.

23 transcription factor steroidogenic factor 1, P450scc converts cholesterol to pregnenolone.

24 The mRNA for cytochrome P450scc (converts cholesterol to pregnenolone), 3beta-hy

25 The expression of mRNA for cytochrome P450scc (converts cholesterol to pregnenolone), 3beta-hy

26 Cytochrome P450 side chain cleavage (P450scc) converts cholesterol to pregnenolone, the rate-

27 nduced transcriptional activity of a porcine P450scc core promoter luciferase construct containing th

28 esterol side chain cleavage cytochrome P450 (P450scc; CYP11A) catalyzes the first step in the product

29 ement of the steroidogenic enzyme cytochrome P450scc (CYP11A1) as well as CYP27B1 (1alpha-hydroxylase

30 Cytochrome P450scc (CYP11A1) catalyzes conversion of cholesterol (C

31 ents using purified mitochondrial cytochrome P450scc (CYP11A1) reconstituted with the iron-sulfer pro

32 t inhibit mitochondrial import of cytochrome P450scc (cytochrome P450 side chain cleavage enzyme) and

33 the placental strategy for transcription of P450scc employs cis-acting elements different from those

34 Moreover, purified mammalian P450scc enzyme and, most importantly, mitochondria isola

35 regnenolone, i.e. the genes and proteins for P450scc enzyme, adrenodoxin, adrenodoxin reductase and M

36 The P450scc-expressing vector (rAAV2-P450scc) or control GFP

37 erotype 2 viral vector (rAAV2), which drives P450scc expression and neuroactive steroid synthesis.

38 esence of aminoglutethimide, an inhibitor of P450scc, FeSO4 increased the synthesis of both steroids,

39 ng conserved lysine residues also crucial in P450scc for binding adrenodoxin.

40 rt presents the first sequence of cytochrome P450scc from this evolutionary unique taxon of vertebrat

41 Adrenal and gonadal strategies for P450scc gene transcription are essentially identical and

42 the transcriptional activity of the porcine P450scc IGFRE by preventing IGF-I-stimulated binding of

43 facilitating the delivery of cholesterol to P450scc in the inner mitochondrial membranes.

44 terol side-chain cleavage enzyme, cytochrome P450scc, initiates the biosynthesis of all steroid hormo

45 criptional activity of the porcine P-45011A (P450scc) insulin-like growth factor response element (IG

46 uces predominantly the hydroperoxy-ferriheme P450scc intermediate, along with a minor fraction of per

47 During annealing of the hydroperoxy-ferric P450scc intermediates at 185 K, they convert to the prim

48 erase chain reaction indicated that mRNA for P450scc is more abundant than mRNA for both P450c17 and

49 Because placental expression of P450scc is required for human pregnancy, we sought facto

50 experiments in granulosa cells with deletion P450scc/luciferase constructs showed that TNFalpha inhib

51 reported pathway of vitamin D3 metabolism by P450scc may have wider biological implications depending

52 ulin-like growth factor-I (IGF-I)-stimulated P450scc mRNA concentrations in cultures of porcine granu

53 ong with a loss of adrenal steroids, whereas P450scc mRNA decreased by less than 2-fold.

54 mo-cAMP-induced P(4) production and StAR and P450scc mRNA expression.

55 However, we find that P450scc mRNA is up-regulated in thymocytes on the initia

56 mino terminus longer than the other forms of P450scc, no translation initiation signal (ATG) was evid

57 tating delivery of cholesterol to cytochrome P450scc on the inner mitochondrial membrane.

58 The P450scc-expressing vector (rAAV2-P450scc) or control GFP-expressing vector (rAAV2-GFP) we

59 These results suggest that P450scc overexpression and the resultant increase of 3al

60 P450scc overexpression in the NAc, however, did not alte

61 P450scc overexpression in the VTA significantly reduced

62 P450scc overexpression produced a 36% increase in (3alph

63 The relative abundance of mRNA for P450scc, P450c17, and steroidogenic factor 1 in SEB-1 se

64 B (anti-HBsAg) and membrane-bound cytochrome P450scc (P450scc).

65 adrenodoxin than another mitochondrial P450, P450scc (product of the CYP11A1 gene).

66 at bind to the -155/-131 region of the human P450scc promoter, which participates in its placental bu

67 Furthermore, the activity of a P450SCC promoter/luciferase reporter construct, which is

68 ol to the inner mitochondrial membrane where P450scc resides.

69 Thus, 7-DHC can form 7-DHP through P450scc side-chain cleavage, which may serve as a substr

70 in order to facilitate investigation of the P450scc system.

71 he full cytochrome P450 side-chain cleavage (P450scc) system required for the intracellular catalytic

72 heir modulation of placental but not adrenal P450scc transcription underscores the distinctiveness of

73 , a specific 252 bp fragment of the putative P450scc was amplified from RNA of interrenal tissue (the

74 RNA for cytochrome P450 side-chain cleavage (P450scc) was also down-regulated by MIS.

75 Thus, in contrast to P450scc, where mutation of the two conserved positively

76 (StAR) and P450 side-chain cleavage enzyme (P450scc), without affecting P450 aromatase mRNA level, s