ライフサイエンスコーパス: P5CDH

1 rvation of different oligomeric states among P5CDHs suggests potential differences in cooperativity a

2 Within each protomer, the PRODH and P5CDH active sites face each other at a distance of 41 A

3 (PutA) whereas in other organisms PRODH and P5CDH are expressed as separate monofunctional enzymes.

4 In Gram-negative bacteria, PRODH and P5CDH are fused together in the bifunctional enzyme prol

5 known x-ray crystal structures of PRODH and P5CDH from T. thermophilus, a model was built for a prop

6 c data for the coupled reaction of PRODH and P5CDH from Thermus thermophilus are consistent with a su

7 In contrast, two Bacillus P5CDHs form dimers in solution but do not assemble into

8 hogen Cryptococcus neoformans, and bacterial P5CDHs have been targeted for vaccine development.

9 and quaternary structures of four bacterial P5CDHs using a combination of small-angle X-ray scatteri

10 ta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH) activities.

11 ta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH) activities.

12 ta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH) catalyze the two-step oxidation of proline to glu

13 ta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH) catalyzes the NAD(+)-dependent oxidation of gluta

14 ta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH) domains that catalyze the oxidation of l-proline

15 ta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH) domains.

16 ta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH), a stress-related gene, and SRO5, a gene of unkno

17 as 1-pyrroline-5-carboxylate dehydrogenase (P5CDH), and the homologous immunogen in S. pneumoniae as

18 ta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDH; also known as ALDH4A1), the aldehyde dehydrogenas

19 pyrroline-5-carboxylate (P5C) dehydrogenase (P5CDH) catalyze the four-electron oxidation of proline t

20 Here, we report the first structure of human P5CDH (HsP5CDH) and investigate the impact of the hyperp

21 metabolic disorder type II hyperprolinemia, P5CDH is essential for virulence of the fungal pathogen

22 Defects in P5CDH activity lead to the metabolic disorder type II hy

23 Furthermore, inactive P5CDH and PRODH mutants inhibit NADH production and incr

24 Interestingly, P5CDH binding to PRODH was only observed when PRODH was

25 As anticipated, PutA(669) lacks P5CDH activity.

26 Structures of the 93% identical mouse P5CDH complexed with sulfate ion (1.3 A resolution), glu

27 21-nt siRNAs by DCL1 and further cleavage of P5CDH transcripts.

28 nt for ALDH function, the oligomerization of P5CDH has remained relatively unstudied.

29 te and transient kinetic data for the PRODH, P5CDH, and coupled PRODH-P5CDH reactions was used to tes

30 evidence of substrate channeling in a PRODH-P5CDH two-enzyme pair.

31 The coupled PRODH-P5CDH activity of PutA is best described by a mechanism

32 ver kinetic experiments of the coupled PRODH-P5CDH reaction revealed that the rate of NADH formation

33 data for the PRODH, P5CDH, and coupled PRODH-P5CDH reactions was used to test various models describi

34 s, indicating that the mutants disrupt PRODH-P5CDH channeling interactions.

35 enzymes is conserved in monofunctional PRODH-P5CDH enzyme pairs.

36 he first kinetic model for the overall PRODH-P5CDH reaction of a PutA enzyme.

37 ilus, a model was built for a proposed PRODH-P5CDH enzyme channeling complex.

38 of 3 mum was estimated for a putative PRODH-P5CDH complex by surface plasmon resonance (SPR).

39 mediate in coupled assays of wild-type PRODH-P5CDH enzyme pairs, indicating that the mutants disrupt

40 In the P5CDH active site, l-glutamate-gamma-semialdehyde (the h

41 Initial cleavage of the P5CDH transcript guided by the 24-nt siRNA establishes a

42 Our data suggest that the P5CDH and SRO5 proteins are also functionally related, a

43 are also functionally related, and that the P5CDH-SRO5 gene pair defines a mode of siRNA function an

44 e transport of glutamate semialdehyde to the P5CDH active site.

45 arboxylate, is preferably transferred to the P5CDH domain rather than released into the bulk medium.

46 The P5CDHs from Thermus thermophilus and Deinococcus radiodu

47 membrane association and communication with P5CDH.