ライフサイエンスコーパス: PACS

1 PACS and CR provide cost savings only when a 12-year har

2 PACS provides a general new technical capability that ex

3 PACS was achieved by PCR with a set of primers to anchor

4 PACS-1 associates with both AP-1 and AP-3, but not AP-2,

5 PACS-1 connects the clathrin adaptor AP-1 to acidic clus

6 PACS-1 directs the TGN localization of furin by binding

7 PACS-1 is a cytosolic protein involved in controlling th

8 PACS-1 is a cytosolic sorting protein that directs the l

9 PACS-1 is an intracellular sorting protein that mediates

10 PACS-1 links GGA3 to CK2, forming a multimeric complex r

11 PACS-1-bound CK2 stimulates GGA3 phosphorylation, releas

12 PACS-2 also controls formation of ER lipid-synthesizing

13 PACS-2 co-localizes with ADAM17 on early endosomes and P

14 PACS-2 controls the apposition of mitochondria with the

15 PACS-2 is phosphorylated at Ser437 in vivo, and pharmaco

16 PACS-2 loss reduces ADAM17 cell-surface levels and ADAM1

17 osphofurin acidic cluster-sorting protein 1 (PACS-1) in the ciliary trafficking of the olfactory cycl

18 osphofurin acidic cluster sorting protein-1 (PACS-1) binding sites in the cytoplasmic tail caused Pol

19 licated in a second independent group of 165 PACS subjects.

20 osphofurin acidic cluster sorting protein 2 (PACS-2), mitofusins (Mfn1/2), and dynamin related protei

21 osphofurin acidic cluster sorting protein-2 (PACS-2) to acidic residues in the carboxyterminal tail o

22 osphofurin acidic cluster sorting protein-2 (PACS-2) to DR5-positive endosomes in Huh-7 cells where i

23 f 71 AAC eyes, 71 fellow eyes of AAC, and 25 PACS eyes were recruited.

24 A PACS workstation significantly decreased the delays in o

25 e TGN localization of furin is directed by a PACS-1-mediated retrieval step.

26 The use of a PACS can result in the elimination of multiple time-inte

27 Use of a PACS improves the delivery of chest images, facilitates

28 examination was taken, and the presence of a PACS workstation influenced the time from radiographic e

29 taken at night or day, and the presence of a PACS workstation were significant predictors of the elap

30 ngles on paracoronal reformations by using a PACS angle tool.

31 iography (CR) hard copy, was compared with a PACS, which used CR images displayed on a multiviewer in

32 Additionally, PACS should aid in the identification of cell-specific o

33 up expense increased by a mean of 146% after PACS implementation from 4,221 dollars per 1,000 studies

34 5 during transition and 32, 22, and 18 after PACS implementation, with a maximum increase of as much

35 1 during transition and 53, 49, and 50 after PACS implementation, which resulted in a maximum increas

36 for the Nef-SFK interaction or the AP-1 and PACS-1-dependent sequestering of internalized MHC-I, sug

37 sly unidentified cargo subsite on PACS-1 and PACS-2 interacted with a bipartite site on Nef formed by

38 of Nef with the sorting proteins PACS-1 and PACS-2 mediates key signaling and trafficking steps requ

39 ef and the PACS proteins on Rab5 (PACS-2 and PACS-1)- or Rab7 (PACS-1)-positive endosomes as determin

40 ower in AAC eyes than fellow eyes of AAC and PACS eyes (p < 0.01 for both) and AAC eyes demonstrated

41 osure and compared to fellow eyes of AAC and PACS eyes.

42 ACD was less in AACG than CACG and PACS (P < .001).

43 ignificantly more in AACG eyes than CACG and PACS eyes (P < .001 and P = .007, respectively).

44 idely) acquisition, processing, display, and PACS software--with 4 sites stating that poor-quality im

45 localizes with ADAM17 on early endosomes and PACS-2 knockdown decreases the recycling and stability o

46 iated by GGA3, which directs TGN export, and PACS-1, which directs endosome-to-TGN retrieval.

47 anterior lens position than fellow eyes and PACS eyes, though lens thickness did not differ among th

48 e executing opposing sorting steps, GGA3 and PACS-1 bind to an overlapping CI-MPR trafficking motif a

49 Here, we show that Nef and PACS-1 combine to usurp the ARF6 endocytic pathway by a

50 genes could be so easily identified because PACS preferentially identifies coding instead of non-cod

51 ng pathway is not restricted to Nef, because PACS-2 is also required for trafficking of an endocytose

52 incidental findings increased from 19 before PACS to 31 during transition and 53, 49, and 50 after PA

53 follow-up studies increased from five before PACS to 15 during transition and 32, 22, and 18 after PA

54 p mutation increased the interaction between PACS-1 and cargo, whereas a Ser278-->Ala substitution de

55 Angle closure was more prevalent in both PACS siblings (35.0%) and PAC/PACG siblings (36.7%) comp

56 is dependent on CK2 phosphorylation of both PACS-1 and its cargo.

57 Only 1 eye of CACG, PACS, and control subjects were selected.

58 ure was 13.6 times greater in angle closure (PACS or PAC/PACG) siblings compared with OA siblings (95

59 ctivated phosphorylation cascade controlling PACS-1- and GGA3-mediated CI-MPR sorting.

60 ng site 72PXXP(75), and M(20)-in controlling PACS-1-dependent sorting to the TGN, ARF6 activation, an

61 ts as an autoregulatory domain that controls PACS-1-directed sorting.

62 Together, these results demonstrate PACS-2 is required for Nef action and sorting of itinera

63 s suggests that TRAIL/DR5 triggers endosomal PACS-2 to recruit Bim and Bax to lysosomes to release ca

64 useful software that can be used to enhance PACS performance.

65 ghest in AACG eyes, followed by fellow eyes, PACS, and CACG.

66 e role of one member of a novel gene family, PACS-1, in the localization of trans-Golgi network (TGN)

67 ffect major purchase decisions, not only for PACS but also for other supporting software and for moda

68 ACS-2 apoptotic activity and is required for PACS-2 to mediate trafficking of membrane cargo.

69 hese studies demonstrate the requirement for PACS-1 interactions with adaptor proteins in multiple pr

70 and its parts and on supporting software for PACS.

71 re incurred by the radiology department from PACS implementation.

72 te-based angle calculations will differ from PACS angle measurements by no more than 4.02 degrees .

73 responsible for imaging capture either from PACS system or directly from an MRI scanner, or from raw

74 g splenocytes and embryonic fibroblasts from PACS-2(-/-) mice, we confirm genetically that Nef requir

75 ompression will experience cost savings from PACS.

76 Full PACS-CR implementation would not provide cost savings fo

77 reas an increase in expression of functional PACS-1 leads to an increase in HCMV titer, suggesting th

78 Hence, PACS-2 sustains ADAM17 cell-surface activity by divertin

79 Together, our results identify PACS-2 as a novel sorting protein that links the ER-mito

80 Here we identify PACS-2 as an essential TRAIL effector, required for kill

81 imate the odds of prevalent angle closure in PACS or PAC/PACG siblings compared with OA siblings.

82 ntation and caused a Nef mutant defective in PACS binding to localize to distorted endosomal compartm

83 a significant increase in the angle width in PACS.

84 However, in response to apoptotic inducers, PACS-2 translocates Bid to mitochondria, which initiates

85 Multi-PACS was applied between a normal osteoblast and four OG

86 The extended method is termed multi-PACS.

87 ction, while adenoviral expression of mutant PACS-1 causes similar mislocalization.

88 Consequently, disruption of the Nef-PACS interaction repressed Nef-induced MHC-I down-regula

89 about the molecular basis underlying the Nef-PACS interaction.

90 Expression of dominant-negative PACS-1 causes a mislocalization of both furin and mannos

91 Furthermore, expression of dominant-negative PACS-1 inhibits the ability of HIV-1 Nef to downregulate

92 on of this motif yielded a dominant-negative PACS-1 molecule that can still bind to acidic cluster mo

93 78-->Ala mutation yields a dominant-negative PACS-1 molecule that selectively blocks retrieval of PAC

94 otype of the more severely affected eye: OA, PACS, or PAC/PACG.

95 % of PAC/PACG siblings compared with 4.9% of PACS siblings (P = 0.07) and 0% of OA siblings (P = 0.00

96 rylates PACS-1Ser(278), promoting binding of PACS-1 to CI-MPR to retrieve the receptor to the TGN.

97 of mitochondria with the ER, as depletion of PACS-2 causes BAP31-dependent mitochondria fragmentation

98 0.6), gender (P = 0.7), and distribution of PACS versus PAC or PACG (P = 0.7).

99 bolic homeostasis, whereas downregulation of PACS-2 or IP3R1, proteins important for ER-mitochondria

100 (65), with the furin binding region (fbr) of PACS-1 is crucial for this Nef function.

101 contact was present in approximately half of PACS eyes and approximately two thirds of PAC/PACG eyes

102 Furthermore, inhibition of PACS-1 activity in infected cells leads to a decrease in

103 We have now investigated the interaction of PACS-1 with heterotetrameric adaptor complexes.

104 The introduction of PACS into radiology practice for lumbar spinal MR imagin

105 embrane permeabilization, shRNA knockdown of PACS-2 in Huh-7 cells reduced TRAIL-induced apoptosis an

106 ting that short interfering RNA knockdown of PACS-2 phenocopies the gene knock-out.

107 shRNA-targeted knockdown of PACS-2 prevents recruitment of Bim or Bax to lysosomes,

108 Moreover, genetic loss of PACS-2 or inhibition of class I PI3K prevents Nef-mediat

109 the homeostatic and apoptotic properties of PACS-2 that mediate TRAIL action.

110 olecule that selectively blocks retrieval of PACS-1-regulated cargo molecules to the TGN.

111 of having PAC/PACG compared with siblings of PACS probands, although the association was not signific

112 tudies identify the phosphorylation state of PACS-2 Ser437 as a molecular switch that integrates cell

113 analysis identified 3 distinct subgroups of PACS subjects based on AS-OCT and biometric parameters.

114 his study was part of a prospective trial of PACS.

115 s stating that poor-quality images appear on PACS.

116 Here we show that an acidic cluster on PACS-1, which is highly similar to acidic cluster sortin

117 tor, but not TGN46, is strictly dependent on PACS-1.

118 ich is the first of two parts, will focus on PACS and its parts and on supporting software for PACS.

119 A previously unidentified cargo subsite on PACS-1 and PACS-2 interacted with a bipartite site on Ne

120 ture archiving and communication systems (or PACS), ultrasonography, computed tomography, magnetic re

121 Bound CK2 also phosphorylates PACS-1Ser(278), promoting binding of PACS-1 to CI-MPR to

122 transition and 27, 17, and 14 in the 3 post-PACS years.

123 s statistically significant during each post-PACS year.

124 udies increased the most from two in the pre-PACS year to 11 during transition and 27, 17, and 14 in

125 m 4,221 dollars per 1,000 studies in the pre-PACS year to 9,307 dollars, 13,426 dollars, 10,558 dolla

126 lation state-dependent furin-sorting protein PACS-1, and mirrors the trafficking pathway described re

127 n and interacts with the TGN sorting protein PACS-1.

128 iRNA screen, we identify the sorting protein PACS-2 as a regulator of ADAM17 trafficking and ErbB sig

129 65)-dependent binding to the sorting protein PACS-2 targets Nef to the paranuclear region, enabling P

130 cation of a multifunctional sorting protein, PACS-2, that integrates ER-mitochondria communication, E

131 interaction of Nef with the sorting proteins PACS-1 and PACS-2 mediates key signaling and trafficking

132 We show that CNGB1b contains two putative PACS-1 binding sites, which are phosphorylated by the se

133 n between Nef and the PACS proteins on Rab5 (PACS-2 and PACS-1)- or Rab7 (PACS-1)-positive endosomes

134 oteins on Rab5 (PACS-2 and PACS-1)- or Rab7 (PACS-1)-positive endosomes as determined by bimolecular

135 The determination that recombinant PACS-2 bound Bim but not Bax in vitro and that shRNA kno

136 s 14-3-3 with high affinity, which represses PACS-2 apoptotic activity and is required for PACS-2 to

137 s dephosphorylation of Ser437, reprogramming PACS-2 to promote apoptosis.

138 ce, we confirm genetically that Nef requires PACS-2 to localize to the paranuclear region and assembl

139 institution into the receiving institution's PACS significantly decreased the rates of subsequent ima

140 ferential amplification of coding sequences (PACS) to screen multiple samples simultaneously.

141 ferential amplification of coding sequences (PACS), that was applied to identify differentially expre

142 Analyses in vitro and in vivo show PACS-1 has properties of a coat protein and connects fur

143 Since PACS is not dependent on the existence of a poly A tail

144 Our approach, PCR-activated cell sorting (PACS), uses TaqMan PCR to detect nucleic acids within si

145 ACG) eyes, 40 primary angle-closure suspect (PACS) eyes, and 27 normal eyes underwent complete examin

146 egorized with primary angle-closure suspect (PACS), and 16 persons (0.8%) were categorized with prima

147 anagement of primary angle closure suspects (PACS), chronic primary angle closure (CPAC), and chronic

148 a cohort of primary angle-closure suspects (PACS).

149 picture archiving and communication system (PACS) without being printed to film, and 108 were printe

150 picture archiving and communication system (PACS) workstation in the MICU shortens those delays.

151 picture archiving and communication system (PACS) workstation, with standard, locally determined cen

152 e picture archival and communication system (PACS) workstation.

153 picture archiving and communication system (PACS) workstations equipped with software for two- and t

154 picture archiving and communication system (PACS), as well as some of the useful software that can b

155 picture archiving and communication systems (PACS) are a major focus of imaging informatics, there ar

156 It was also less in fellow eyes than PACS eyes (P = .04).

157 e HCMV envelope glycoprotein B (gB) and that PACS-1 function is required for normal TGN localization

158 o more than 5 degrees and 95% confident that PACS tool-based angle measurements will differ from the

159 Additionally, we demonstrate that PACS can be used to sort and enrich cells via TaqMan PCR

160 We show here that PACS-1 interacts with the cytoplasmic domain of the HCMV

161 Additionally, we show that PACS-1 is expressed in OSNs and interacts in complex wit

162 Superresolution imaging showed that PACS-2- and Mfn-mediated membrane apposition or hydropho

163 o an increase in HCMV titer, suggesting that PACS-1 is required for efficient production of HCMV.

164 The PACS tool-based measured and coordinate-based calculated

165 revented the interaction between Nef and the PACS proteins on Rab5 (PACS-2 and PACS-1)- or Rab7 (PACS

166 Here we identify the sites on Nef and the PACS proteins required for their interaction and describ

167 amates in Nef do not functionally engage the PACS-1 fbr.

168 h a greater percentage of angles open in the PACS group compared with the PAC/PACG group (52.4% vs. 3

169 ced by 45% with direct image transfer to the PACS compared with the time required in the film-based m

170 (i) The binding of Nef to the PACS-1 fbr in vitro is much weaker than the binding of t

171 the binding of the canonical furin AC to the PACS-1 fbr.

172 o obtain information during periods with the PACS workstation compared with periods without the works

173 2c did not interfere with the PACS-2-dependent trafficking of Nef required for the Nef

174 Without the PACS workstation, higher occupancy, higher aggregate sev

175 This PACS-2-dependent targeting pathway is not restricted to

176 Thus, PACS identified CSTs approximately 13.5 times more often

177 n 1487 consecutive ED patients, CD import to PACS was attempted between February 1 and August 31, 200

178 CD import to PACS was successful in 78% (1161 of 1487) of patients.

179 reports for each of 5 years: 1 year prior to PACS introduction, 1 year during transition to PACS, and

180 CS introduction, 1 year during transition to PACS, and 3 consecutive years thereafter.

181 d after implementation of an enterprise-wide PACS; the numbers and types of imaging examinations perf

182 apital and operational costs associated with PACS implementation during an 8-year time horizon.

183 Seventy-nine eyes with PACS, CPAC, or CPACG and better than 20/50 visual acuity

184 These acidic cluster motifs interact with PACS-1, a connector protein that is required for the tra

185 and two acidic clusters, which interact with PACS-1, a cytosolic protein, to mediate retrograde trans

186 i-detector row scanners and interpreted with PACS workstations enable depiction of pancreas divisum.

187 In a South Indian population with PACS or PAC/PACG, LPI results in significant anterior ch

188 A short sequence within PACS-1 that is essential for binding to AP-1 has been id