ライフサイエンスコーパス: PAM

1 PAM 11 was very active in feeding inhibition in rodents,

2 PAM acts as an allosteric effector and triggers the inte

3 PAM neurons show increased activity following caffeine a

4 PAM sequences are known to vary considerably between sys

5 PAM-1 lacking both glycosylation sites (PAM-1/OSX; where

6 PAM-ABP and siRNA formed polyplexes with average diamete

7 PAM-SCANR and the PAM wheel identified known functional

8 PAMs and NAMs of mGluRs, either of which can inhibit evo

9 PAMs from pigs that possessed the hCD163L1 domain 8 homo

10 We report 2 PAM cases, 1 fatal and 1 surviving, treated with the nov

11 pared to the current antidote pralidoxime (2-PAM).

12 e found that FnCas9 recognizes the 5'-NGG-3' PAM, and used the structural information to create a var

13 s to edit endogenous sites with the 5'-YG-3' PAM, thus expanding the target space of the CRISPR-Cas9

14 that can recognize the more relaxed 5'-YG-3' PAM.

15 Moreover, 3D PAM images can be useful in understanding vessel-related

16 Et is a novel chemical structure acting as a PAM at particular GABAA receptors.

17 yl)isoxazole (CMPI) has been identified as a PAM selective for neuronal nAChRs containing thealpha4 s

18 The combination of a PAM and low levels of exogenous GABA further increased h

19 Here, we find evidence of a PAM-induced allosteric mechanism revealed by microsecond

20 lex does not require a target sequence (or a PAM) in the DNA substrate.

21 ombined properties of agonists and PAMs (ago-PAM) and produce very effective channel activation (dire

22 that Cas proteins can be engineered to alter PAM recognition, opening new opportunities to develop CR

23 ied Cas9 and its VQR variant with an altered PAM specificity.

24 resolution structures delineated the altered PAM recognition mechanisms of the AsCpf1 variants, provi

25 Although PAM recognition is essential for cleavage, it is unknown

26 Although PAMs must work in concert with "orthosteric" agonists, c

27 also eukaryotic phenylalanine aminomutases (PAMs) for the synthesis of the (R)-beta-products.

28 ound that nucleotides at both PAM-distal and PAM-proximal regions of the sgRNA are significantly corr

29 t displayed both high intrinsic efficacy and PAM activity, those that showed no detectable agonism bu

30 FbaA and PAM (i.e., the M53 protein) had additive effects in prom

31 ive allosteric modulators (an mGluR5 NAM and PAM) for TSC, using a mutant mouse model with neuronal l

32 s all wavelengths (Flambda , 670-850 nm) and PAM fluorescence parameters under a range of actinic lig

33 for association with pregnancy outcomes and PAM indicators using linear and logistic multivariate mo

34 talytic function, guide RNA specificity, and PAM requirements and reducing off-target activity for th

35 have the combined properties of agonists and PAMs (ago-PAM) and produce very effective channel activa

36 ctivity of GABAAR agonists, antagonists, and PAMs on diverse GABAAR isoforms.

37 findings indicate that free DNA termini and PAMs are conserved features important for CRISPR spacer

38 We applied PAM to image the vascular morphology, blood oxygenation,

39 9 specificity wherein gRNA-DNA mismatches at PAM-distal bases modulate different biophysical paramete

40 The 5'-ATG PAM is recognized in duplex form, from the minor groove

41 first small molecule modulator to attenuate PAM complex activity, likely through binding to the C-te

42 unanticipated, highly conserved link between PAM, amidation and ciliary assembly.

43 g frames, that are identical (or have BLOSUM/PAM similarity that exceeds a user-specified value) to t

44 We found that nucleotides at both PAM-distal and PAM-proximal regions of the sgRNA are sig

45 phy (NIR-VISPAOCT) system that combines both PAM and OCT with a conventional surgical microscope.

46 ate that AcrIIA4 competitively occupies both PAM-interacting and non-target DNA strand cleavage catal

47 sites with both canonical and non-canonical PAMs.

48 Chlamydomonas PAM knockdown lines failed to assemble cilia beyond the

49 d high molecular weight complexes containing PAM-1; the ability of PAM-1/OSX to form similar complexe

50 PAM, Cpf1 recognizes suboptimal C-containing PAMs.

51 In contrast, PAM-distal mismatches up to 11 base pairs in length, whi

52 s finer lateral resolution than conventional PAM.

53 times the depth of field of the conventional PAM counterpart.

54 nontarget sequences associated with correct PAMs.

55 iew, we discuss the properties of the CRISPR PAM and the emerging tools for determining, visualizing,

56 When placed in culture, PAMs from pigs with the CD163 KO phenotype were complete

57 Decreased PAM gene expression reduced motile ciliary density on th

58 The results demonstrated PAM cells inoculated with PRRSV followed by PCV2 2 h lat

59 jugated poly (amidoamine) (PAMAM) dendrimer (PAM-ABP) in hMSCs.

60 tems, comparing cleavage using a SNP-derived PAM to a guide specific approach.

61 In vitro, cleavage via a SNP-derived PAM was found to confer stringent allele-specific cleava

62 cade complex can respond to several distinct PAM sequences.

63 BMS-986187 is a structurally distinct PAM for the delta-opioid receptor (delta-OR) that has be

64 These distinct PAMs also exhibited differential impacts during synaptic

65 th outcome in pregnant women with documented PAM.

66 says to show that BMS-986187 is an effective PAM at the mu-OR and at the kappa-opioid receptor (kappa

67 or determining, visualizing, and engineering PAM recognition.

68 NA binding to PAM favors separation of a few PAM-proximal protospacer base pairs allowing initial tar

69 ally targets critical residues essential for PAM duplex recognition, as well as blocks target DNA acc

70 Our work opens new perspectives for PAM in biomedical sciences.Photoacoustic microscopy allo

71 regulator of fast conduction physiology for PAM and VCS cardiomyocytes.

72 d DNA beyond a short threshold distance from PAM into the Cas9/single-guide RNA (sgRNA) interior is h

73 nt of the cytosolic domain was produced from PAM-1/OSX.

74 NOT-gate repression) to elucidate functional PAMs as well as an interactive visualization scheme term

75 nd the PAM wheel identified known functional PAMs while revealing complex sequence-activity landscape

76 We identify a large number of functional PAMs that can promote interference, priming or both acti

77 ns of elucidating and visualizing functional PAMs toward accelerating our ability to understand and e

78 This adduct undergoes further PAM via reaction of the remaining three ligands with TCN

79 f NMDARs and AMPA receptors (AMPARs), GluN2A PAMs with good selectivity against AMPARs were identifie

80 Individual GluN2A PAMs exhibited variable degrees of glutamate (Glu) depen

81 for cleavage, it is unknown whether and how PAM binding activates Cas9 for DNA cleavage at spatially

82 supports the interest of a serotonin 5-HT2CR PAM as a promising therapeutic approach for obesity.

83 FI-FH proteolytically cleaves C3b into iC3b, PAM-bound hPm catalyzes cleavage of iC3b into multiple s

84 Introducing the identified PAM-interacting mutations at their corresponding positio

85 , these studies show that we have identified PAMs of the D2 and D3 receptors both in vitro and in viv

86 on and, consequently, are classed as type II PAMs.

87 compare with other alpha7-selective type II PAMs.

88 In PAM-1/OSX, a cleavage site for furin-like convertases wa

89 Cas9 tolerance to single-base mismatches in PAM-distal region than to PAM-proximal region and the hi

90 me termed the PAM wheel to convey individual PAM sequences and their activities.

91 from recent studies of systemically injected PAMs and NAMs of mGluRs in rodents and monkeys, focusing

92 Internalized PAM-1/OSX was rapidly degraded; unlike PAM-1, very littl

93 aded; unlike PAM-1, very little internalized PAM-1/OSX was detected in multivesicular bodies.

94 ng positions in LbCpf1 similarly altered its PAM specificity.

95 thologs, in complex with a guide RNA and its PAM-containing DNA targets.

96 ivity at a wide variety of receptors, lacked PAM activity at related Gi-coupled G protein-coupled rec

97 by P. falciparum protein VAR2CSA, a leading PAM-specific vaccine target.

98 We observed a repeat length-, PAM-, and strand-dependent reduction of repeat-containin

99 n, we describe the characterization of an M1 PAM radioligand, 8-((1S,2S)-2-hydroxycyclohexyl)-5-((6-(

100 is a strong positive correlation between M1 PAM binding affinity and functional activity, and that [

101 he design and synthesis of lactam-derived M1 PAMs that address this hypothesis.

102 en affinity values generated for nineteen M1 PAMs in the [(3)H]PT-1284 binding assay and the EC50 val

103 binding site, as well as profiling novel M1 PAMs.

104 se model and support a role for selective M1 PAMs as a novel approach for the treatment of schizophre

105 pocampal muscarinic signaling using M1 mAChR PAMs restored memory loss and slowed the progression of

106 aracterization of a novel family of M1 mAChR PAMs.

107 or pharmacological investigation of M1 mAChR PAMs.

108 ere compared to porcine alveolar macrophage (PAM) in terms of surface marker phenotype, susceptibilit

109 stablished the porcine alveolar macrophages (PAM) cells model co-infected with PRRSV/PCV2 with modifi

110 n of primary pulmonary alveolar macrophages (PAMs) also induces interferons.

111 ctions between porcine alveolar macrophages (PAMs) and wild-type A. pleuropneumoniae (5b WT) or an Ad

112 phenotyping of porcine alveolar macrophages (PAMs) showed that pigs with the KO or SRCR domain 5 dele

113 Pregnancy associated malaria (PAM) causes adverse pregnancy and birth outcomes owing t

114 Pregnancy-associated malaria (PAM) is associated with poor pregnancy outcomes.

115 The potential available market (PAM) for new diagnostics for tuberculosis that meet the

116 ster of neurons, the paired anterior medial (PAM) cluster of dopaminergic neurons, as the ones releva

117 red to as the protocerebral anterior medial (PAM) cluster.

118 Primary amebic meningoencephalitis (PAM) is a fulminant central nervous system infection cau

119 disease primary amoebic meningoencephalitis (PAM) and can be found in drinking water systems in many

120 ection, primary amoebic meningoencephalitis (PAM) induced by Naegleria fowleri is extremely lethal, w

121 riven selection would have resulted in mGlu2 PAMs with high values for kon but not necessarily optimi

122 fforts leading to potent and selective mGlu2 PAMs.

123 ach used in its development, our novel mGlu5 PAM improves RS phenotypes and synaptic plasticity defec

124 trophysiology to determine whether the mGlu5 PAM VU0409551 directly enhances NMDAR function in hippoc

125 pport for the continued development of mGlu5 PAMs as potential therapeutic agents for use in RS, and,

126 mproved solubility compared with other mGlu5 PAMs, thus allowing brain-site-specific pharmacological

127 monkey using a structurally distinct mGluR2 PAM ligand with affinity for the same site, demonstratin

128 mGluR2, was evaluated as a selective mGluR2 PAM PET tracer.

129 nt fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed i

130 omography (OCT) or photoacoustic microscopy (PAM) has been independently combined with conventional s

131 Photoacoustic microscopy (PAM) images the ocular vasculature without using exogeno

132 Photoacoustic microscopy (PAM) is uniquely positioned for biomedical applications

133 cage 1 undergoes post-assembly modification (PAM) via a Diels-Alder cycloaddition of the anthracene p

134 ultaneously with pulse-amplitude modulation (PAM) and spectrally resolved fluorescence over the same

135 The novel positive allosteric modulator (PAM) 1-(4-(2,4-difluorophenyl) piperazin-1-yl)-2-((4-flu

136 es with mGlu2 positive allosteric modulator (PAM) activity and affinity.

137 nly exhibited positive allosteric modulator (PAM) activity.

138 ignaling with positive allosteric modulator (PAM) blocked aberrant LTP in dSPNs, enabled LTP reversal

139 ype-selective positive allosteric modulator (PAM) contributes to the gastrointestinal (GI) and cardio

140 of M1 with a positive allosteric modulator (PAM) has emerged as a new approach to achieve selective

141 (mAChR) via a positive allosteric modulator (PAM) is a new approach for the treatment of the cognitiv

142 esting that a positive allosteric modulator (PAM) of alpha7 receptors, JNJ-39393406, may aid smoking

143 shown to be a positive allosteric modulator (PAM) of GABA currents with alpha1beta2gamma2, alpha1beta

144 S-986122 is a positive allosteric modulator (PAM) of the mu-opioid receptor (micro-OR).

145 hat acts as a positive allosteric modulator (PAM) of the rat and human dopamine D2 and D3 receptors.

146 ective mGluR4 positive allosteric modulator (PAM) with good water solubility and demonstrating consis

147 ffects of the positive allosteric modulator (PAM), ML380, and negative allosteric modulator, ML375.

148 etrant mGluR2 positive allosteric modulator (PAM), SAR218645.

149 a novel mGlu5 positive allosteric modulator (PAM), termed VU0462807, can rescue synaptic plasticity d

150 lly active M1 positive allosteric modulator (PAM), VU0453595, allowed us to evaluate the impact of se

151 hCB2 receptor positive allosteric modulator (PAM).

152 Positive allosteric modulators (PAM) of metabotropic glutamate receptor 2 (mGluR2) are a

153 a series of positive allosteric modulators (PAMs) acting on the human alpha7 nicotinic acetylcholine

154 velopment of positive allosteric modulators (PAMs) and negative allosteric modulators (NAMs) of mGluR

155 or 5 (mGlu5)-positive allosteric modulators (PAMs) are currently being developed to treat cognitive d

156 ly selective positive allosteric modulators (PAMs) of M1 mAChRs.

157 Positive allosteric modulators (PAMs) of nicotinic acetylcholine (ACh) receptors (nAChRs

158 Positive allosteric modulators (PAMs) of nicotinic acetylcholine receptors (nAChR) are i

159 Novel positive allosteric modulators (PAMs) of NMDARs have recently been identified but their

160 e identified positive allosteric modulators (PAMs) of NMDARs with selectivity for GluN2A subunit-cont

161 Positive allosteric modulators (PAMs) of the M1 muscarinic acetylcholine receptor (M1 mA

162 A number of positive allosteric modulators (PAMs) that enhance GABA's actions on neuronal GABAA-Rs a

163 to selective positive allosteric modulators (PAMs), which increase the efficiency of channel activati

164 NARW movements, passive acoustic monitoring (PAM) was used in this study in order to better capture y

165 ptidylglycine alpha-amidating monooxygenase (PAM) (EC 1.14.17.3) catalyzes peptide amidation, a cruci

166 ptidylglycine alpha-amidating monooxygenase (PAM), an enzyme required for generating amidated bioacti

167 and flanked by a protospacer adjacent motif (PAM) and is widely used for genome editing in various or

168 sence of a short protospacer adjacent motif (PAM) flanking the target site, and subsequent R-loop for

169 rement of a TTTV protospacer adjacent motif (PAM) in the DNA substrate.

170 avage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA target sequence, thus const

171 he presence of a protospacer adjacent motif (PAM) next to the target site.

172 s in the seed or protospacer adjacent motif (PAM) of the target sequence cause immune failure and all

173 A protospacer adjacent motif (PAM) sequence flanking target DNA is crucial for self ve

174 s with different protospacer-adjacent motif (PAM) specificities to expand the number of sites that ca

175 from a 5'-CCN-3' protospacer adjacent motif (PAM) that is critical for invader targeting.

176 rches gRNA by 3' protospacer-adjacent motif (PAM), and possible off-targets, and scores the conservat

177 ches proximal to protospacer-adjacent motif (PAM), demonstrating that mismatches encountered early du

178 quence, called a protospacer adjacent motif (PAM), for target recognition.

179 sequences as the protospacer adjacent motif (PAM), thereby expanding the target range of Cpf1-mediate

180 trategy based on Protospacer Adjacent Motif (PAM)-altering SNPs to target patient-specific CRISPR/Cas

181 isruption of the protospacer adjacent motif (PAM).

182 izes an extended protospacer adjacent motif (PAM).

183 ntation from the protospacer adjacent motif (PAM).

184 ommonly rely on protospacer-adjacent motifs (PAMs) as the first step in target recognition.

185 systems rely on protospacer-adjacent motifs (PAMs) for DNA target recognition.

186 and destroying protospacer adjacent motifs (PAMs).

187 hich we refer to as periodic axial motility (PAM), is controlled by c-Src and MEK1/2-ERK1/2.

188 in of Tim44 of the protein-associated motor (PAM) complex.

189 Etv1 was highly expressed in murine PAM and VCS cardiomyocytes, where it regulates expressio

190 ng property of pectinated atrial myocardium (PAM) and the ventricular conduction system (VCS) and is

191 support further testing of this alpha7 nAChR PAM compound for possible efficacy in smoking cessation,

192 , an engineered Cas9-VQR variant with 5'-NGA PAM specificities is used to induce base conversion in z

193 er improved by introducing an additional non-PAM-interacting mutation.

194 t complexes containing PAM-1; the ability of PAM-1/OSX to form similar complexes was markedly diminis

195 demonstrates that a large-scale analysis of PAM data provides significant value to understanding and

196 e for controlled endoproteolytic cleavage of PAM and the separation of soluble peptidylglycine alpha-

197 We report two consequences of PAM-distal mismatches: reversal of dCas9 binding at long

198 t evidence of radical-induced degradation of PAM under HPT hydraulic fracturing conditions without ad

199 tly it catalyzed the chemical degradation of PAM, likely due to dissolution of Fe(2+) at low pH.

200 soluble fragment of the cytosolic domain of PAM-1 was produced in the endocytic pathway and entered

201 ansfection efficiency and VEGF expression of PAM-ABP using gWiz-Luc and pbeta-VEGF were higher than t

202 o-immunoprecipitation of a small fraction of PAM and Atp7a supports the suggestion that copper can be

203 SX; where OSX is O-glycan-depleted mutant of PAM-1) was stably expressed in AtT-20 corticotrope tumor

204 riginal cage, demonstrating the potential of PAM for tuning the binding properties of supramolecular

205 s, O-glycans may facilitate the recycling of PAM-1 through the endocytic compartment.

206 management tools for mitigating the risks of PAM.

207 se selection for proof-of-concept studies of PAM compounds.

208 PAM-1/OSX differed dramatically from that of PAM-1.

209 The endocytic trafficking of PAM-1/OSX differed dramatically from that of PAM-1.

210 This novel class of PAMs shows distinct effects on synaptic plasticity.

211 tool for interpreting functional effects of PAMs.

212 Consensus docking with an extended series of PAMs with chemical similarity to A-867744, TBS-516, and

213 RISPR/Cas9 guide RNAs (gRNAs) that depend on PAM sites generated by SNP alleles on the mutant chromos

214 exist on the impact of these Hb variants on PAM.

215 onstrate that, contrary to many reports, one PAM-proximal mismatch is insufficient to abolish dCas9-m

216 Optimal PAM recognition coincides with wedge insertion, initiati

217 associated with other pregnancy outcomes or PAM indicators.

218 Polyacrylamide (PAM) based friction reducers are a primary ingredient of

219 ion coefficients of Pu(V) in polyacrylamide (PAM) gel in the presence of humic acid using a diffusion

220 We went on to identify a more potent PAM for use in native receptor systems.

221 tudies of the M1 mAChR with the prototypical PAM, BQZ12.

222 Furthermore, a GABAA-R PAM promoted human islet cell replication in vitro.

223 ain a past clinical observation of a GABAA-R PAM reducing HbA1c levels in diabetic patients.

224 , we show that clinically applicable GABAA-R PAMs can increase significantly INS-1 ss-cell replicatio

225 Repurposing these GABAA-R PAMs to help treat diabetes is theoretically appealing b

226 of this endogenous hormone-like CB2 receptor PAM, with unforeseen opposite allosteric effects on cann

227 reatments were the alpha7 nicotinic receptor PAM JNJ-39393406 (100 mg b.i.d.) or placebo (double-blin

228 m) directly via an hPg/hPm surface receptor (PAM), we show that both FI-FH and hPm sequentially cleav

229 DNA duplexes, thereby achieving the relaxed PAM recognition.

230 ype III-A CRISPR-Cas system does not require PAM or seed sequences, and thus prevents viral escape vi

231 that showed no detectable agonism but robust PAM activity and ligands that displayed robust allosteri

232 d garnered insights into SpyCas9 and SauCas9 PAM diversity.

233 sitive, and tunable screen termed PAM-SCANR (PAM screen achieved by NOT-gate repression) to elucidate

234 is way, both microscopic and cross-sectional PAM and OCT images are concurrently displayed on the ocu

235 ctor, it can back-project 2D cross-sectional PAM and OCT images onto the microscopic view plane.

236 The compound behaves as a selective PAM of mGluR2 in recombinant and native receptor express

237 (PF-06767832) is a high quality M1 selective PAM that has well-aligned physicochemical properties, go

238 bioavailable, and CNS-penetrant M1-selective PAM with minimal agonist activity.

239 udies have focused on three alpha7-selective PAMs (A-867744, TBS-516, and TQS) that display similar e

240 ite and characterization of GluN2A-selective PAMs provide powerful molecular tools to dissect NMDAR f

241 M1 selective PAMs were shown to inhibit [(3)H]PT-1284 binding in a co

242 which is flanked by single guide RNA (sgRNA)-PAM sequences and is released after CRISPR/Cas9 cleavage

243 GABAA receptors, while having no significant PAM effect on alphabeta receptors or alpha1beta1gamma2,

244 Further, SIR-PAM achieves 1.5 times finer lateral resolution than con

245 o realize motionless volumetric imaging, SIR-PAM combines two-dimensional Fourier-spectrum optical ex

246 ant resolution photoacoustic microscopy (SIR-PAM).

247 PAM-1 lacking both glycosylation sites (PAM-1/OSX; where OSX is O-glycan-depleted mutant of PAM-

248 The shale sample adsorbed some PAM ( approximately 30%), but importantly it catalyzed t

249 In the early steps, PAM recognition causes severe DNA bending, leading to sp

250 Subsequently, PAMs apoptosis via the activation of the Fax and Bax sig

251 R/K548V/N552R, which recognize TYCV and TATV PAMs, respectively, with enhanced activities in vitro an

252 odulated chlorophyll fluorescence technique (PAM) allows quantitative leaf level monitoring of the ut

253 in vivo, positive, and tunable screen termed PAM-SCANR (PAM screen achieved by NOT-gate repression) t

254 Here, we show that PAM is required for the normal assembly of motile and pr

255 Our data suggest that PAM activity and alterations in post-Golgi trafficking c

256 We conclude that PAMs and NAMs of mGluRs should be considered for clinica

257 These findings suggest that PAMs may potentiate the actions of GABA secreted by isle

258 The PAM wheel was also readily applicable to existing high-t

259 PAM-SCANR and the PAM wheel identified known functional PAMs while reveali

260 The identity of the PAM and the PAM-proximal nucleotides control Cas3 recruitment by rel

261 ning either TTTA, TCTA, TCCA, or CCCA as the PAM.

262 The results by the PAM (Partitioning Around Medoids) approach, in particula

263 is study also supports a direct role for the PAM complex in the import of substrates that are lateral

264 % inhibitory concentration = 9.6 nM) for the PAM site of mGluR2, was evaluated as a selective mGluR2

265 uplex DNA binding promote DNA bending in the PAM-proximal region during early steps of Cas9/guide RNA

266 Notably, the PAM-interacting cleft adopts a "locked" conformation on

267 t the S isomer attenuated the effects of the PAM and the effects of dopamine.

268 The identity of the PAM and the PAM-proximal nucleotides control Cas3 recrui

269 of the DNA sequence and the distance of the PAM site from the nucleosome dyad.

270 d 25 bp upstream and 40 bp downstream of the PAM site, while 20-30 bp of the binding site itself are

271 y analyses, the peak molecular weight of the PAM was reduced by 2 orders of magnitude, from roughly 1

272 advance our mechanistic understanding of the PAM-dependent, crRNA-guided DNA cleavage by the Cpf1 fam

273 e structures revealed that, depending on the PAM sequences, LbCpf1 undergoes conformational changes t

274 so propose a standard means of orienting the PAM to simplify how its location and sequence are commun

275 RVR and RR variants primarily recognize the PAM-complementary nucleotides via the substituted residu

276 enomic site as well as those surrounding the PAM region are a major component of the prediction capab

277 interactive visualization scheme termed the PAM wheel to convey individual PAM sequences and their a

278 e the structural basis for understanding the PAM-dependent directional R-loop formation process.

279 hanges to form altered interactions with the PAM-containing DNA duplexes, thereby achieving the relax

280 The PAMs showed various kinetic profiles; kon values ranged

281 However, the PAMs did potentiate [(3)H]-dopamine binding at both D2 a

282 ound that Adh was shown to interact with the PAMs membrane protein OR5M11, an olfactory receptor, res

283 However, their PAM recognition mechanisms remained unknown.

284 These PAMs lacked activity at a wide variety of receptors, lac

285 Due to evidence that these PAMs bind within the alpha7 nAChR transmembrane region,

286 er can be transferred directly from Atp7a to PAM, a process that can occur only when both proteins ar

287 results show that Cas9/guide RNA binding to PAM favors separation of a few PAM-proximal protospacer

288 ed segments of nontarget strand DNA close to PAM.

289 base mismatches in PAM-distal region than to PAM-proximal region and the high specificity and efficie

290 Besides the canonical TTTV PAM, Cpf1 recognizes suboptimal C-containing PAMs.

291 Whereas wild-type AsCpf1 recognizes the TTTV PAM, the RVR (S542R/K548V/N552R) and RR (S542R/K607R) va

292 can efficiently recognize the TATV and TYCV PAMs, respectively.

293 lized PAM-1/OSX was rapidly degraded; unlike PAM-1, very little internalized PAM-1/OSX was detected i

294 This project used PAM data from 2004 to 2014 collected by 19 organizations

295 These studies were conducted using PAMs that also exhibited allosteric agonist activity, le

296 circulation in the eye by combining in vivo PAM imaging and an ocular surface estimation method base

297 hemical degradation of high molecular weight PAM, including the effects of shale, oxygen, temperature

298 kely to be the same transmembrane site where PAMs like PNU-120596 function.

299 lso review results from rat studies in which PAMs or NAMs of mGluRs were injected intracranially to r

300 ice (Oryza sativa) in canopy conditions with PAM fluorescence under natural solar radiation.