ライフサイエンスコーパス: PAS domain

1 a diminished inhibitory interaction with the PAS domain.

2 s in Escherichia coli with an FAD-containing PAS domain.

3 will adopt a fold similar to the ubiquitous PAS domain.

4 e periplasmic (PP) domain, and a cytoplasmic PAS domain.

5 nges in the N-terminal cap (Ncap) of the VVD PAS domain.

6 , which has both a HAMP domain and a sensory PAS domain.

7 vin adenine dinucleotide cofactor bound to a PAS domain.

8 -sheet interface between the monomers of the PAS domain.

9 redox potential via FAD bound to a cytosolic PAS domain.

10 of N- or C-terminal helices attached to the PAS domain.

11 portant for stabilizing the structure of the PAS domain.

12 at GAC63 interacts with AHR through its bHLH-PAS domain.

13 face for the interaction with the HIF-2alpha PAS domain.

14 AMP domain that abolished FAD binding to the PAS domain.

15 he Aer sensor is an N-terminal, FAD-binding, PAS domain.

16 r PDE8A1 and depended on the presence of the PAS domain.

17 vs) that recognize different epitopes on the PAS domain.

18 lators of hERG that specifically bind to the PAS domain.

19 code an [FeFe]-hydrogenase with a C-terminal PAS domain.

20 in both isolated and tandem arrangements of PAS domains.

21 olution of ligand recognition and binding by PAS domains.

22 tem via the FAD cofactor associated with its PAS domains.

23 allosteric switching that is conserved among PAS domains.

24 asparagine receptor McpB revealed two tandem PAS domains.

25 e closely resembles prokaryotic heme-binding PAS domains.

26 rotein with the mixed alpha/beta topology of PAS domains.

27 ns containing the basic-helix-loop-helix and PAS domains.

28 noticeably shorter than those of the typical PAS domains.

29 are regulated by an N-terminal Per-Arnt-Sim (PAS) domain.

30 the presence of an N-terminal Per-Arnt-Sim (PAS) domain.

31 PYP is also the prototypical Per-Arnt-Sim (PAS) domain.

32 acterized by the presence of a Per-Arnt-Sim (PAS) domain.

33 th basic helix-loop-helix/Per-Arnt-Sim (bHLH-PAS) domains.

34 de (FMN) adduct within sensory Per-ARNT-Sim (PAS) domains.

35 entrainment requires expression of Neuronal PAS domain 2 (Npas2) and Period2 (Per2) genes, component

36 YybT homologs contain a small Per-ARNT-Sim (PAS) domain (~80 amino acids) that can bind b-type heme

37 tio via binding of NAD(+) to the kinase in a PAS domain A-dependent manner.

38 The PAS domain (a sensory domain named after three proteins

39 OX domains (one with heme, one without), two PAS domains, a coiled-coil domain, and two cyclase domai

40 In oxygen-sensing PAS domains, a conserved polar residue on the proximal s

41 ygen binding (H-NOX) domain, a Per/ARNT/Sim (PAS) domain, a coiled-coil (CC) domain, and a catalytic

42 ase predicted to have multiple Per/Arnt/Sim (PAS) domains, a histidine kinase domain and a C-terminal

43 sequence level, the structures of dozens of PAS domains across a broad section of sequence space hav

44 implications for the molecular mechanism of PAS domain activation and indicate that stimulus-induced

45 PAS domain activation often involves conformational chan

46 or function by the interaction with the bHLH-PAS domain (AD3) of p160 coactivators.

47 both PAS and GAF domains, rather than in the PAS domain alone as in wild-type BphPs.

48 Both domains adopt the typical PAS domain alpha/beta topology and are structurally simi

49 taining an ACT domain, a connecting helix, a PAS domain and a C-terminal helix.

50 ge-charge interaction between Arg(56) of the PAS domain and Asp(803) of the cNBH domain, as well an a

51 rongly protected two regions of the sGCbeta1 PAS domain and caused local structural relaxation in oth

52 d the region encoding residues 14-119 of the PAS domain and found 72 aerotaxis-defective mutants, 24

53 In ReFixL, the core heme-containing PAS domain and kinase region is preceded by an N-termina

54 or kinase, CtcB, possessed an energy-sensing PAS domain and phosphorylation site.

55 inding of FAD to Aer requires the N-terminal PAS domain and residues in the F1 region and C-terminal

56 co-factor located within the amino-terminal PAS domain and responds to the fixed nitrogen status by

57 ose that functional interactions between the PAS domain and the HAMP AS-2 helix are required for FAD

58 ctivity was also shown to depend on the WalK PAS domain and to limit cross-talk and the recovery of W

59 toplasmic BdlA protein harboring two sensory PAS domains and a chemoreceptor domain, TarH.

60 rotein assumes the global fold common to all PAS domains and dimerizes via a hydrophobic interface.

61 late these changes to those in other dimeric PAS domains and discuss the role of quaternary structura

62 ose described in other crystal structures of PAS domains and identifies a conserved dimerization moti

63 t establish DNA binding and the stability of PAS domains and pockets.

64 new insight into the architecture of tandem PAS domains and suggests specific residues that may play

65 or functional interactions between the N-Cap/PAS domains and the cNBH domain.

66 domain, which is composed of a Per-Arnt-Sim (PAS) domain and a PAS-cap domain, whereas the carboxy-te

67 RmcA contains four Per-Arnt-Sim (PAS) domains and domains with the potential to catalyze

68 n lesions mimic the 'signal-on' state of the PAS domain, and therefore may be markers for the signal-

69 the complex formed in solution by these two PAS domains, and confirm the validity of this model usin

70 hich dimerize via basic helix-loop-helix and PAS domains, and recruit coactivators via HIF-alpha C-te

71 s Sma0113, an HWE histidine kinase with five PAS domains, and Sma0114, a CheY-like response regulator

72 op-helix (bHLH) domain and two Per-Arnt-Sim (PAS) domains, and HdHIF-1alpha has a oxygen-dependent de

73 oiled-coil platform upon which the H-NOX and PAS domains are assembled.

74 In the bacterial kingdom, PAS domains are commonly positioned at the amino terminu

75 PAS domains are defined by a small number of conserved r

76 The beta1 H-NOX and alpha1 PAS domains are in contact and form the core signaling c

77 nt and could differ depending on whether the PAS domains are isolated or are part of a full-length pr

78 PAS domains are often dimeric and act as versatile senso

79 Multiple PAS domains are often found within a single protein.

80 Physically, HIF1alpha HLH and PAS domains are required for its interaction with STAT3

81 Per/Arnt/Sim (PAS) domains are ubiquitous sensors in thousands of spec

82 lK(Spn) phosphatase activity depended on the PAS domain as well as residues in the DHp domain.

83 ed by the N-terminal N-Cap and Per-Arnt-Sim (PAS) domains, as well as the C-terminal cyclic nucleotid

84 omplex containing the mouse CLOCK:BMAL1 bHLH-PAS domains at 2.3 A resolution.

85 experiments suggest that XREs, E-boxes, and PAS domain/basic helix-loop-helix transcription factors

86 The basic helix-loop-helix PAS domain (bHLH-PAS) transcription factor CLOCK:BMAL1 (

87 In other proteins, PAS domains bind ligands and modulate effector domains.

88 -HAMP function, we determined that the Aer-2 PAS domain binds penta-co-ordinated b-type haem and that

89 Spectroscopy reveals that whereas the PAS domain binds to both the ferric and ferrous forms of

90 nscription factor; the AhR Per-AhR/Arnt-Sim (PAS) domain binds ligands.

91 arginine and tyrosine residues of the upper PAS domain but not in any residues of the lower PAS doma

92 similar to those of other flavin-containing PAS domains, but homodimeric interactions in other struc

93 e of the N-cap (residues 1 to 19) in the Aer PAS domain by missense and truncation mutagenesis.

94 annel function through ligand binding to the PAS domain can be attained.

95 Our data reveal that the ubiquitous PAS domain can make the transition from sensor to enzyme

96 f ligand affinity and signal transduction by PAS domains can be direct or indirect.

97 g interactions as observed for several other PAS domain complexes.

98 Because disruption of individual PAS domains compromise HIF function independent of the m

99 PAS domain containing protein kinase (Pask) is an evolut

100 The basic helix-loop-helix (bHLH) -PAS domain containing transcription factors CLOCK and BM

101 he aryl hydrocarbon receptors (AHR) are bHLH-PAS domain containing transcription factors.

102 ins, CetA (HAMP domain containing) and CetB (PAS domain containing).

103 e sensor domains are the first structures of PAS domains containing covalently bound heme.

104 nvironmental ligand-dependent, per ARNT-sim (PAS) domain containing bHLH transcription factor that me

105 trh encodes a basic helix-loop-helix PAS-domain containing transcription factor that has been

106 investigation of the role of the endothelial PAS domain-containing protein 1 (EPAS1), a regulatory al

107 -gamma, coactivator 1 (Pgc-1alpha), neuronal PAS domain-containing protein 2 (Npas2), and retinoic ac

108 In this review, we summarize the roles of PAS domain-containing proteins in mammals.

109 Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) is an evolu

110 eurospora circadian clock, the PER-ARNT-SIM (PAS) domain-containing transcription factor, WHITE COLLA

111 cloche gene and discovered that it encodes a PAS-domain-containing bHLH transcription factor, and tha

112 esis analogous to the functions performed by PAS-domain-containing regulatory proteins found in compl

113 We show that upon citrate binding, the PAS domain contracts, resulting in a shortening of the C

114 Here we show that CRY1 binds directly to the PAS domain core of CLOCK:BMAL1, driven primarily by inte

115 ponicum is a PAS sensor protein in which two PAS domains covalently linked to a histidine kinase doma

116 tilis, we constructed a series of systematic PAS domain deletion mutants and analyzed their activitie

117 It consists of an N-terminal heme-bound PAS domain, denoted bjFixLH, and a C-terminal histidine

118 The more C-terminal PAS domain, denoted bjFixLH, contains a heme cofactor th

119 The crystal structure of the MmoS tandem PAS domains, designated PAS-A and PAS-B, has been determ

120 odules, such as the widespread Per-ARNT-Sim (PAS) domains, detect signals and, in response, regulate

121 esent homology models of the murine AhR:ARNT PAS domain dimer developed using recently available X-ra

122 ion, early doors (Edo), in the PER-ARNT-SIM (PAS) domain dimerization region of period 2 (PER2) (I324

123 hat permit small-molecule binding, including PAS domain encapsulated sites and an interfacial cavity

124 domain but not in any residues of the lower PAS domain exhibited a chemotactic defect in both swarm

125 onto the transcription factor alongside the PAS domains, extending above the DNA-binding bHLH domain

126 t of 63 PAS structures demonstrates that the PAS domain family forms structural clades on the basis o

127 ll-molecule metabolites is a hallmark of the PAS domain family.

128 ive yellow protein (PYP), a prototype of the PAS domain family.

129 o be distinct from the superficially similar PAS domain fold.

130 l aromatic hydrocarbon-sensing Per-Arnt-Sim (PAS) domain, followed by an autokinase domain, a respons

131 ironment and require a motif external to the PAS domain for proper folding.

132 Sma0113 may use its five PAS domains for redox level or energy state monitoring a

133 of erWalK revealed a canonical Per-Arnt-Sim (PAS) domain for signal sensing.

134 PAS domains, found in diverse multi-domain proteins from

135 Here we present crystal structures of PAS domain fragments of mPER1 and mPER3 and compare them

136 of the heterodimer formed by the C-terminal PAS domains from the HIF2alpha and ARNT subunits of the

137 The universal Per-ARNT-Sim (PAS) domain functions as a signal transduction module in

138 The PAS domain has also been implicated in the assembly and

139 ytic activity, to our knowledge no enzymatic PAS domain has been found.

140 at least twice in evolutionary history, the PAS domain has been lost and it is omitted by alternate

141 However, the function(s) of the PAS domain has remained unknown.

142 ired to mediate other HIF functions in which PAS domains have been implicated.

143 The crystal structure of the heme-binding PAS domain in the ferric, ligand-free form, in compariso

144 of the environmental sensing role played by PAS domains in a wide range of proteins, including other

145 Given the essential role of PAS domains in forming active HIF heterodimers, these re

146 KinA contains three PAS domains in its amino-terminal sensor domain, which a

147 To characterize the role of the three PAS domains in KinA, the major sporulation kinase in Bac

148 , as also borne out by comparison to several PAS domains in which mutations leading to disruption of

149 show that both antibodies, on binding to the PAS domain, increase the total amount of current that pe

150 on, we propose a model for the mode of multi-PAS domain interaction in bHLH-PAS transcriptional activ

151 The second PAS domain interacts with the asymmetrically partitioned

152 The phyA PAS domain interacts with the COP1 WD40 domain.

153 Putting the extracytoplasmic PAS domain into context of the membrane-embedded CitA co

154 Intriguingly, the hallmark PAS domain is dispensable for LIN-42 function in transge

155 The PAS domain is linked by an F1 region to a membrane ancho

156 antitative Western blotting to show that the PAS domain is not required for normal channel traffickin

157 However, when the PAS domain is present, the N-Cap amphipathic helix must

158 abilization of full-length proteins when the PAS domain is present.

159 An FAD-binding PAS domain is the sensor for Aer and a HAMP domain inter

160 The results showed that any one of the three PAS domains is sufficient to maintain the kinase activit

161 The Per-ARNT-Sim (PAS) domain is a conserved alpha/beta fold present withi

162 The Per-Arnt-Sim (PAS) domain is a ubiquitous protein module with a common

163 The Per-Arnt-Sim (PAS) domain is conserved across the kingdoms of life and

164 the Helix-Loop-Helix (HLH) and PER-ARNT-SIM (PAS) domains, is needed to convert the AhR into its tran

165 s that, in contrast to previous studies, the PAS domain itself is extended by approximately 3 nm (at

166 elices, with RsbQ hydrolase activity and the PAS domain jointly comprising a positive sensing module

167 Mutations located on one face of the PAS domain (K28E, F29L, N33T, R56Q, and M124R) caused de

168 eractions between the region upstream of the PAS domain knot and the bilin A and B pyrrole rings.

169 However, its Per/Arnt/Sim (PAS) domain, knot region, and helical spine show distinc

170 small percentage of hERG channels containing PAS domain LQT2 mutations (hERG PAS-LQT2) have been char

171 and CW-biased mutations suggest that the Aer PAS domain may engage in two different interactions with

172 these data indicate that PDE8A1, through its PAS domain, may bind with IkappaB proteins in a region c

173 These include the PAS-domain metabolic sensor kinase Psk2, the mitochondri

174 S-like domains do not match sequence-derived PAS domain models, and thus their distribution across th

175 reconstituted on liposomes, we show that one PAS domain modulates kinase activity in a CckA density-d

176 A deletion of the N-terminus PAS domain, mutation R4AR5A or the LQT2-causing mutation

177 We used a genetically encoded hERG PAS domain (NPAS) to examine whether channel dysfunction

178 Per-Arnt-Sim (PAS) domains occur in proteins from all kingdoms of life

179 the solution structure of the corresponding PAS domain of ARNT and show that it utilizes a very simi

180 All these mutations were in the cytoplasmic PAS domain of EvgS, and were shown to be solely responsi

181 The N-terminal PAS domain of FixL contains a histidine-coordinated heme

182 We determined the solution structure of the PAS domain of GtYybT from Geobacillus thermodenitrifican

183 mined the crystal structure of the FAD-bound PAS domain of NifL from Azotobacter vinelandii to 1.04 A

184 ellular redox is monitored by the N-terminal PAS domain of NifL which contains an FAD cofactor.

185 ceptor function by interacting with the bHLH-PAS domain of p160 coactivators.

186 BDSF is shown to bind to the PAS domain of RpfR with high affinity and stimulates its

187 the Aer dimer stabilized FAD binding to the PAS domain of the cognate monomer.

188 ind an internal cavity within the C-terminal PAS domain of the HIF-2alpha subunit.

189 Here it is shown that the cytoplasmic PAS domain of this multi-domain transmembrane kinase is

190 d kinase region is preceded by an N-terminal PAS domain of unknown function and followed by a C-termi

191 e report here that CoCoA also binds the bHLH-PAS domains of AHR and ARNT and functions as a potent pr

192 We have previously shown that the C-terminal PAS domains of an HIF-alpha isoform (HIF-2alpha) and ARN

193 However, the PAS domains of KCNH channels are orphan receptors.

194 primary structure within the two N-terminal PAS domains of LovhK have distinct sensory roles under s

195 The PAS domains of other PAS proteins are soluble in water.

196 to recent models, our data reveal that both PAS domains of the HIF-alpha subunit are necessary for h

197 Flavin-based PAS domains of this type have also been referred to as L

198 and its M domain bound to the Per-Arnt-Sim (PAS) domain of apo-sGC-beta1(1-359), which lies adjacent

199 eract directly with the second PER-ARNT-SIM (PAS) domain of ARNT (ARNT PAS-B).

200 The C-terminal Per-ARNT-Sim (PAS) domain of HIF2alpha (HIF2alpha PAS-B) contains a pr

201 racytoplasmic, citrate-sensing Per-Arnt-Sim (PAS) domain of HK CitA are identical for the isolated do

202 al basic-helix-loop-helix/Per-Arnt-Sim (bHLH-PAS) domain of p160 coactivators is unknown.

203 domain used to associate with the analogous PAS domain on its heterodimeric bHLH-PAS partner HIF-2al

204 support a regulatory model in which the heme-PAS domain operates as an ensemble that couples to the k

205 onal enzyme and that the most amino-terminal PAS domain (PAS-A) plays an important role in sensing th

206 nsible for redox sensing, whereas the second PAS domain, PAS2, has no apparent cofactor and its funct

207 Per-Arnt-Sim (PAS) domains play a critical role in signal transduction

208 nducible factor (HIF) 1alpha and endothelial PAS domain protein 1 (EPAS1 or HIF2alpha), which are ind

209 subunits, HIF1A (HIF-1alpha) and endothelial PAS domain protein 1 (EPAS1; HIF-2alpha), are overexpres

210 muscle Arnt-like protein 1 (Bmal1), neuronal PAS domain protein 2 (Npas2) and cryptochrome 1 (Cry1),

211 -PAS domain proteins like mammalian neuronal PAS domain protein 2 (NPAS2) and the direct oxygen senso

212 s recapitulated with a knockdown of neuronal PAS domain protein 2 (NPAS2) specifically in the NAc, de

213 s kaput (Clock) in combination with neuronal PAS domain protein 2 (Npas2), induced severe age-depende

214 Mice missing the gene encoding neuronal PAS domain protein 3 (NPAS3) are devoid of hippocampal n

215 The neuronal PAS domain protein 3 (NPAS3) gene encoding a brain-enric

216 In mice, loss of neuronal PAS domain protein 3 (NPAS3) impairs postnatal hippocamp

217 Neuronal PAS domain protein 4 (Npas4), a recently discovered IEG,

218 unction of the novel beta-cell IEG, neuronal PAS domain protein 4 (Npas4).

219 he transcription factor (TF) NPAS4 (neuronal PAS domain protein 4) has been found to provide activity

220 transcription factor encoded by endothelial PAS domain protein-1 (EPAS1).

221 wo basic helix-loop-helix PER-ARNT-SIM (bHLH-PAS) domain protein subunits, CLOCK and BMAL1.

222 basic helix-loop-helix (bHLH), Per-Arnt-Sim (PAS) domain protein, a novel type of hormone receptor.

223 romoter is selectively activated by neuronal PAS-domain protein 2 (NPAS2)/BMAL1.

224 RcoM-1 and BxRcoM-2, are gas-responsive heme-PAS domain proteins like mammalian neuronal PAS domain p

225 Acting through intestinal bHLH-PAS domain proteins Methoprene-tolerant (Met) and Germ c

226 The neuronal PAS domain proteins NPAS1 and NPAS3 are members of the b

227 nisms of AhR transformation, dimerization of PAS domain proteins, and Hsp90 dissociation in activatio

228 eral members of the core clock mechanism are PAS domain proteins, one of which, neuronal PAS 2 (NPAS2

229 anges in PYP plus commonalities shared among PAS domain proteins, we further propose that PAS domains

230 r N-terminal helices are identified in other PAS domain proteins.

231 ominant regulatory effect was exerted by the PAS domain proximal to the effector domain.

232 mutants that probe different regions of the PAS domain quantify the anisotropy in stability and chan

233 s interaction is regulated by the PAS kinase PAS domain, raising the possibility that this interactio

234 specific DivL mutants revealed that the DivL PAS domains regulate binding specificity for DivK approx

235 proteins, extracytoplasmic sigma factors and PAS-domain regulators.

236 protein variants strongly suggested that the PAS domain residues His74 and Met104 serve as the heme F

237 alanine replacement mutagenesis of the BdlA PAS domain residues previously demonstrated to be essent

238 c channel, with many clinical mutants in the PAS domain resulting in reduced stability of the domain

239 Three amino acid replacements in the Aer-PAS domain, S28G, A65V, and A99V, restored FAD binding a

240 haracterization of the newly discovered heme-PAS domain sensor protein BxRcoM-2 reveals that this pro

241 ne kinase upstream of CtrA, employs a tandem-PAS domain sensor to integrate two distinct spatiotempor

242 PAS domain proteins, we further propose that PAS domains share this conformational mechanism, which e

243 ctural data on natural receptors with tandem PAS domains show that these are predominantly linked by

244 In contrast, absence of the HAMP-PAS domains significantly diminished the phosphatase act

245 In contrast, PAS domains (similar to GAF domains in structure but not

246 ha subunit reveals that it is a haem-binding PAS domain, similar in structure to PAS gas sensors.

247 ts from NMR spectroscopy illustrate how this PAS domain simultaneously mediates interactions with HIF

248 A deletion of the first PAS domain strongly increased the oxygen affinity but es

249 where ligand binding induces alterations in PAS domain structure and subunit association that is rel

250 sensing domain exhibits a dual Per-Arnt-Sim (PAS) domain structure.

251 r within its N-terminal tandem Per-Arnt-Sim (PAS) domains, suggesting that it functions as a redox se

252 a 125-residue prototype of the PER-ARNT-SIM (PAS) domain superfamily of signaling proteins.

253 ithin the first of two amino-terminal tandem PAS domains, termed PAS1 and PAS2.

254 esting possibility that residues outside the PAS domain that are required for FAD binding are essenti

255 of FAD induce conformational changes in the PAS domain that are transmitted to the HAMP and C-termin

256 chia coli Aer protein contains an N-terminal PAS domain that binds flavin adenine dinucleotide (FAD),

257 The structure contains a dimer of the NifL PAS domain that is structurally very similar to those de

258 The Aer sensor is a cytoplasmic, N-terminal PAS domain that is tethered to the membrane by a 47-resi

259 ors are predicted to surround a cleft in the PAS domain that may bind FAD.

260 volve the hydrophobic pocket within the PER2 PAS domains that in other PAS proteins commonly recogniz

261 a potassium channel contains a Per-Arnt-Sim (PAS) domain that is essential for the unique slow deacti

262 domain (eagD) that contains a Per-Arnt-Sim (PAS) domain that is preceded by a conserved sequence of

263 Thus, in oxygen-sensing PAS domains, the interactions of polar residues with the

264 from Azotobacter vinelandii contains tandem PAS domains, the most N-terminal of which, PAS1, contain

265 e mediated by genes containing Per-Arnt-Sim (PAS) domains, the aryl hydrocarbon receptor (AhR), and A

266 otates, extends and cooperates with a distal PAS domain to bind hypoxia response elements.

267 We developed homology models of the AhR PAS domain to characterize previously observed intra- an

268 for Aer and a HAMP domain interacts with the PAS domain to form an input-output module for signal tra

269 Here we show that ARNT uses a single PAS domain to interact with two coiled coil coactivators

270 exposed hydrophobic patch on the core of the PAS domain to stabilize the structure of this critical g

271 the signal transduction pathway from the Aer PAS domain to the signalling domain.

272 on of allosteric structural changes from the PAS domain to these helices is not clear.

273 which also interact with hsp90 through their PAS domains to control protein partner and small ligand

274 Met and FISC appear to use their PAS domains to form a dimer only in the presence of JH o

275 been shown to use its N-terminal bHLH and/or PAS domains to interact with several transcriptional coa

276 and, more broadly, to the abilities of some PAS domains to regulate their activities in response to

277 Trachealess (Trh) is a PAS domain transcription factor regulating Drosophila tr

278 basic helix-loop-helix (bHLH)-Per-Arnt-Sim (PAS) domain transcription factor BMAL1 is an essential c

279 demonstrating the partial unfolding of their PAS domain upon activation.

280 in an orientation orthogonal to that in the PAS domains via a highly conserved motif, including inva

281 e explored the function(s) of the HIF-2alpha PAS domains via mutational analysis.

282 The PAS domain was also critical for RscS activity; substitu

283 To investigate properties of the PAS domain, we subcloned segments of the aer gene from E

284 Although PAS domains were described as intracellular sensors, rec

285 forms of the R206A and wild-type BjFixL heme-PAS domains were similar, except for a more ruffled porp

286 sults reveal a putative "gating face" in the PAS domain where mutations within this region form funct

287 structure reveals a novel cavity within the PAS domain which contains two water molecules directly c

288 Aer is a flavin adenine dinucleotide-binding PAS domain, which is separated from a HAMP/signaling out

289 We demonstrate that this contraction of the PAS domain, which is well characterized for the isolated

290 t upstream of an N-terminal Period/Arnt/Sim (PAS) domain, which upon removal dramatically accelerates

291 CetB has a PAS domain, while CetA has a predicted transmembrane reg

292 some resemblance to LOV domains, a subset of PAS domains widely involved in signaling.

293 taxis receptor, Aer, has a HAMP domain and a PAS domain with a flavin adenine dinucleotide (FAD) cofa

294 ed that DSF bound to the isolated N-terminal PAS domain with a Kd of 1.4 muM.

295 Together, the results unveil a compact PAS domain with a potential ligand-binding pocket and re

296 ross handshake interaction of the N-terminal PAS domain with alphaF of the opposing subunit.

297 of the aer gene from E. coli that encode the PAS domain with and without His6 tags and expressed the

298 The two PAS domains within ARNT, PAS-A and PAS-B, are essential

299 ional role for transcriptional regulation by PAS domains within bHLH-PAS transcription factors.

300 These results support the importance of PAS domains within KinA and other histidine kinases and