ライフサイエンスコーパス: PC-PLC

1 PC-PLC activation occurred at pH 7.0 and lower, but not

2 PC-PLC activation occurs in the acidified vacuolar envir

3 PC-PLC is made as a proenzyme whose maturation is mediat

4 PC-PLC is made as an inactive proenzyme whose activation

5 PC-PLC requires cleavage of an N-terminal propeptide for

6 PC-PLC secreted into infected cells was immunoprecipitat

7 PC-PLC, encoded by plcB, is a broad-range phospholipase,

8 A PC-PLC inhibitor blocks oxidative glutamate toxicity, an

9 e definitive evidence for the existence of a PC-PLC in eukaryotic cells.

10 ctivation of PC-PLC and that activation of a PC-PLC is an important component of macrophage activatio

11 ed a mutant that makes constitutively active PC-PLC (the plcBDelta pro mutant).

12 pH-regulated release of active PC-PLC is Mpl dependent.

13 Bacterial release of active PC-PLC was dependent on Mpl, a bacterial metalloprotease

14 LC, resulting in bacterial release of active PC-PLC.

15 in, Go, in the regulation of cell growth and PC-PLC-mediated hydrolysis of PC in IIC9 fibroblasts.

16 ology produced by pure HBL, and both HBL and PC-PLC were detected at toxic concentrations in infected

17 Slight hemolysis and PC-PLC activation were found when PlcR-producing B. anth

18 esults describe a novel function for LLO and PC-PLC and suggest that L. monocytogenes may use these v

19 A2 regulates compartmentalization of Mpl and PC-PLC, possibly by influencing cell wall properties and

20 on of mammalian PLC-b2, bacterial PI-PLC and PC-PLC, but not by phospholipase D (PLD).

21 uringiensis genes encoding PI-PLC (plcA) and PC-PLC (plcB).

22 Extraction of bacteria-associated PC-PLC and Mpl required cell wall hydrolysis, but there

23 ese data suggest that bacterially associated PC-PLC and Mpl localize at the cell wall-membrane interf

24 anthracis eliminated the activities of both PC-PLC and SPH, whereas introduction into B. anthracis o

25 s are a phosphatidylcholine phospholipase C (PC-PLC) and a metalloprotease (Mpl).

26 Inhibitors of PC-phospholipase C (PC-PLC) and phospholipase A2 (PLA2) had no effect on con

27 ties of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase (PK) C and enhanced NO produc

28 hosphatidylcholine-specific phospholipase C (PC-PLC) and sphingomyelinase (SPH), encoded by the plc a

29 a monocytogenes broad-range phospholipase C (PC-PLC) during infection of human epithelial cells.

30 C hydrolysis, a PC-specific phospholipase C (PC-PLC) has received relatively little attention.

31 hosphatidylcholine-specific phospholipase C (PC-PLC) is a necessary intermediate in transducing apopt

32 atidylcholine (PC)-specific phospholipase C (PC-PLC) is linked to activation of the p42/44 (ERK) kina

33 The broad-range phospholipase C (PC-PLC) of L. monocytogenes contributes to bacterial esc

34 The broad-range phospholipase C (PC-PLC) of L. monocytogenes contributes to bacterial esc

35 idylcholine (PC)-preferring phospholipase C (PC-PLC) of Listeria monocytogenes plays a role in the ba

36 hosphatidylcholine-specific phospholipase C (PC-PLC) of Pseudomonas fluorescens.

37 hosphatidylcholine-specific phospholipase C (PC-PLC) to the pathogenesis of experimental Bacillus end

38 hosphatidylcholine-specific phospholipase C (PC-PLC), inhibited Ca(2+)-induced 5-HT release to 50% of

39 tors of phosphatidylcholine phospholipase C (PC-PLC), PKC (including pseudosubstrate peptides, cheler

40 s is a secreted broad-range phospholipase C (PC-PLC), the activation of which requires processing of

41 ated in part by a bacterial phospholipase C (PC-PLC), whose activation requires cleavage of an N-term

42 hosphatidylcholine-specific phospholipase C (PC-PLC).

43 osphatidylcholine-selective phospholipase C (PC-PLC).

44 nce factor, the broad-range phospholipase C (PC-PLC).

45 f the bacterial broad-range phospholipase C (PC-PLC).

46 activity of the broad-range phospholipase C (PC-PLC).

47 sphatidylcholine-preferring phospholipase C (PC-PLC).

48 sphatidylcholine-preferring phospholipase C (PC-PLC).

49 shown that the broad-range phospholipase C, PC-PLC, promotes lysis of Henle 407 cell primary vacuole

50 assess the importance of compartmentalizing PC-PLC activity during infection, we created a mutant th

51 oxidative glutamate toxicity, and exogenous PC-PLC potentiates glutamate toxicity.

52 three Legionella enzymes and P. fluorescens PC-PLC share conserved domains also present in uncharact

53 nd Mpl-minus bacteria stained positively for PC-PLC, in contrast to less than 5% in untreated cells.

54 peculate that the additional requirement for PC-PLC activity is for lysis of secondary double-membran

55 nd Bacillus cereus PLC (PLCBc), we generated PC-PLC mutants with altered enzymatic activities and sub

56 These data implicate PC-PLC in cellular and organismic responses to I1-recept

57 armacological approach was used to implicate PC-PLC --> PKC --> NO signaling as being important for t

58 ibutes to B. cereus virulence and implicates PC-PLC and collagenase as additional virulence factors.

59 Using a system for inducible PC-PLC expression in L. monocytogenes, we provide eviden

60 Lastly, by using the inducible PC-PLC expression system, we demonstrate that, in the ab

61 During infection, PC-PLC is activated specifically in acidified vacuoles.

62 Interestingly, PC-PLC activity was constitutively active in the Goalpha

63 we demonstrate that, in the absence of LLO, PC-PLC activity is not only required for lysis of primar

64 rotein acts as lyso-PAF-PLC rather than lyso-PC-PLC or N-SMase in cells.

65 observed that all three Mpl mutants mediate PC-PLC activation when bacteria are grown on semisolid m

66 infected cells, as determined by monitoring PC-PLC maturation and compartmentalization.

67 In this model, neither PI-PLC nor PC-PLC had an effect on the course or severity of experi

68 is inefficient, resulting in accumulation of PC-PLC at the membrane-cell wall interface.

69 tivates the ERK kinases is via activation of PC-PLC and that activation of a PC-PLC is an important c

70 slow response to 5-HT involves activation of PC-PLC by G alpha o to liberate diacylglycerol, which st

71 is involved in the proteolytic activation of PC-PLC.

72 nzyme required for proteolytic activation of PC-PLC.

73 is involved in the proteolytic activation of PC-PLC.

74 ulence of compartmentalizing the activity of PC-PLC during infection.

75 ism by which PrsA2 regulates the activity of PC-PLC.

76 re, our results indicated that the amount of PC-PLC activity is critical for the efficiency of vacuol

77 Total amounts of PC-PLC secreted into infected cells increased several-fo

78 ification, and enzymatic characterization of PC-PLC and SPH from B. cereus and B. anthracis.

79 ition, similarly to Mpl-mediated cleavage of PC-PLC propeptide, Mpl-mediated translocation of PC-PLC

80 Immunofluorescence detection of PC-PLC in infected cells was performed.

81 . monocytogenes strain 10403S, expression of PC-PLC has to increase before or upon entry into human e

82 y can be increased by elevated expression of PC-PLC or Mpl, the enzyme required for proteolytic activ

83 s emphasize the species-specific features of PC-PLC important for growth in mammalian cells.

84 in intracellular pH, and the active form of PC-PLC was the most abundant species detected.

85 The inhibition of PC-PLC uncouples the cystine uptake from glutamate inhib

86 e (D609), a relatively specific inhibitor of PC-PLC.

87 ion of PC-PLC, the bacterial localization of PC-PLC and Mpl was investigated.

88 In this study, the pH-dependent mechanism of PC-PLC activation was investigated by manipulating the i

89 ial cell wall and confirmed that the pool of PC-PLC associated with bacteria was efficiently activate

90 cellular growth, bacteria maintain a pool of PC-PLC that is not accessible to antibodies and that is

91 ormation of a bacterially associated pool of PC-PLC that would readily be accessible for activation a

92 The proforms of PC-PLC and Mpl accumulate at the membrane-cell wall inte

93 2, indicating that neither the propeptide of PC-PLC nor PrsA2 is required for native folding of the c

94 stinct possibility that many of the roles of PC-PLC, especially in cell transformation, may be attrib

95 terize the mechanism regulating secretion of PC-PLC, the bacterial localization of PC-PLC and Mpl was

96 iated via the CDC28-dependent stimulation of PC-PLC activity in a novel cell cycle-regulated signalin

97 ages indicated that the rate of synthesis of PC-PLC exceeded the rate of translocation across the bac

98 Interestingly, synthesis of PC-PLC in the absence of its propeptide lead to the secr

99 LC propeptide, Mpl-mediated translocation of PC-PLC across the bacterial cell wall is pH sensitive.

100 membrane interface and that translocation of PC-PLC across the bacterial cell wall is rate limiting,

101 y, we showed that cell wall translocation of PC-PLC is inefficient, resulting in accumulation of PC-P

102 cted cells, rapid cell wall translocation of PC-PLC is triggered by a decrease in pH and correlates w

103 ide and of Mpl in cell wall translocation of PC-PLC, we generated a cleavage site mutant and a propep

104 gulates the activity of Mpl on itself and on PC-PLC.

105 hospholipases C (PLC), a broad spectrum PLC (PC-PLC) and a phosphatidylinositolspecific PLC (PI-PLC).

106 Analysis of PI-PLC, PC-PLC, and Mpl single and double mutants revealed that

107 ore, tested the effects of D609, a purported PC-PLC-specific inhibitor on the activity of SMS.

108 ll, these results suggest that Mpl regulates PC-PLC translocation across the bacterial cell wall in a

109 er, TNF-alpha induced mPGES-1 by stimulating PC-PLC --> PKC --> NO --> cGMP --> PKG signal transducti

110 centrations of D609 used previously to study PC-PLC (10-50 microg/ml).

111 The data presented here show that PC-PLC also is required in oxidative glutamate-induced p

112 Here, we have shown that PC-PLC is also required for lysis of HEp-2 and HeLa cell

113 We have shown that PC-PLC maturation is regulated by Mpl and pH and that Mp

114 These data suggest that PC-PLC modulates neuronal cell death through a mechanism

115 The PC-PLC inhibitor D609 abolished both responses.

116 fficient translocation of the proform of the PC-PLC cleavage site mutant in a manner that was pH sens

117 oteins, we determined that processing of the PC-PLC propeptide by mature Mpl is also pH sensitive.

118 e), the PI-PLC mutant (BTplcA::lacZ), or the PC-PLC mutant (BTplcB::lacZ).

119 Substitutions D4E and H56Y remodeled the PC-PLC active site to more closely resemble the PLCBc ac

120 ell wall properties and interacting with the PC-PLC propeptide.

121 a is the conclusion that the members of this PC-PLC and phosphatase family possess a novel mechanism

122 imilar amounts of active enzyme as wild-type PC-PLC both in broth culture and intracellularly.

123 e to gentamicin, suggesting that unregulated PC-PLC activity causes damage to host cell membranes.

124 -PLC is secreted in an active state, whereas PC-PLC is secreted as an inactive proenzyme (proPC-PLC)

125 LLO was sufficient for inducing FasL, while PC-PLC synergized with LLO for the induction of FasL exp