ライフサイエンスコーパス: PCNA

1 PCNA has two structurally similar domains (I and II) lin

2 PCNA is a highly conserved sliding clamp protein essenti

3 PCNA is a master regulator of DNA replication and S-phas

4 PCNA is a pivotal component of the replication fork mach

5 We observed increased levels of Ki-67, PCNA and cyclins E/D1 in IBM compared with normals and n

6 y) and short interfering RNA (interleukin-8, PCNA, and Bax), as a validation of effective and functio

7 These findings establish TRAIP as a PCNA-binding ubiquitin ligase with an important role in

8 r synthesis during recombination events in a PCNA interaction-dependent way but independently of its

9 artan (also known as DVC1 and C1orf124) is a PCNA-interacting protein implicated in translesion synth

10 i-recombination function but it also plays a PCNA-independent pro-recombination role, probably by sti

11 All proteins that bind to PCNA do so via a PCNA-interacting peptide (PIP) motif that binds near the

12 tion signals including ERK1/2, p-90RSK, Akt, PCNA, and Ki-67, and a reduction in apoptotic factors su

13 glycosylation following CrP treatment; also, PCNA and vimentin (SMC synthetic marker) expression were

14 Our results suggest that although PCNA is much more competitive than XPA in binding replic

15 oliferation markers (Ki-67, p-Erk, p-Akt and PCNA); (3) decreased apoptosis markers, such as cleaved

16 lencing suppressed LPS-induced TNF-alpha and PCNA increases in human cells.

17 S stimulation in vivo, hepatic TNF-alpha and PCNA responses subsided in Nox4-deficient mice compared

18 angiogenic genes (VEGF, bFGF, TNF-alpha and PCNA) were up-regulated as well.

19 n neurogenic niches revealed by the BLBP and PCNA immunostaining.

20 T2AA decreased PCNA/pol eta and PCNA/REV1 chromatin colocalization but did not inhibit P

21 conserved interaction mode between FANCM and PCNA during replication stress, and suggest that this in

22 her replication proteins, including FEN1 and PCNA.

23 metry, topoisomerases IIalpha and IIbeta and PCNA were noteworthy.

24 luding topoisomerases IIalpha and IIbeta and PCNA, which were found associated with nascent DNA.

25 d that Bub1 can form a complex with LANA and PCNA in KSHV-positive cells.

26 caffold or molecular bridge between LANA and PCNA.

27 caffold or molecular bridge between LANA and PCNA.

28 isolated a complex formed between NEIL1 and PCNA (+/-DNA) using size exclusion chromatography (SEC).

29 Mutant Mlh1-Pms1 and PCNA proteins that were defective for Exo1-independent b

30 An interaction between RTEL1 and PCNA is important to prevent telomere fragility, but how

31 on and tethers it to the leading strand, and PCNA (proliferating cell nuclear antigen) binds tightly

32 ic data showing the requirement for SUMO and PCNA binding for the SDSA role of Srs2, Srs2 displays a

33 133, and 250 in PCNA as IGF-1R targets, and PCNA phosphorylation was followed by mono- and polyubiqu

34 entified Proliferating Cell Nuclear Angigen (PCNA).

35 A-loaded proliferating cell nuclear antigen (PCNA(DNA)) and recruits CRL4(Cdt2).

36 ication, proliferating cell nuclear antigen (PCNA) adopts a ring-shaped structure to promote processi

37 Proliferating cell nuclear antigen (PCNA) and alpha-smooth muscle actin (alphaSMA) double-im

38 -induced proliferating cell nuclear antigen (PCNA) and FANCD2 monoubiquitinations (surrogate markers

39 level of proliferating cell nuclear antigen (PCNA) and minichromosome maintenance 4 (MCM4) proteins w

40 (Pax7), proliferating cell nuclear antigen (PCNA) and nicotinamide phosphoribosyltransferase (Nampt)

41 roteins, Proliferating Cell Nuclear Antigen (PCNA) and Replication Protein A (RPA), which are critica

42 protein proliferating cell nuclear antigen (PCNA) and the scaffold protein Rev1.

43 ntaining proliferating cell nuclear antigen (PCNA) and two non-classical DNA polymerases, Rev1 and DN

44 fied the proliferating cell nuclear antigen (PCNA) as a nIGF-1R-binding partner.

45 NA clamp proliferating cell nuclear antigen (PCNA) associates with the C-terminal domain of Exo1 and

46 eaction: proliferating cell nuclear antigen (PCNA) clamp binding/opening/closure/release, ptDNA bindi

47 gulating proliferating cell nuclear antigen (PCNA) expression and ribosomal RNA (rRNA) synthesis in T

48 A-loaded proliferating cell nuclear antigen (PCNA) for activation.

49 Proliferating cell nuclear antigen (PCNA) forms a trimeric ring that associates with and inf

50 Proliferating cell nuclear antigen (PCNA) forms a trimeric ring that encircles duplex DNA an

51 Ki67 and proliferating cell nuclear antigen (PCNA) immunofluorescence, we determined the duration of

52 Proliferating cell nuclear antigen (PCNA) is a highly conserved protein necessary for proper

53 Proliferating cell nuclear antigen (PCNA) is a protein which is involved in DNA replication

54 Proliferating cell nuclear antigen (PCNA) lies at the center of the faithful duplication of

55 Proliferating cell nuclear antigen (PCNA) loading by replication factor C (RFC) acts as the

56 repair, proliferating cell nuclear antigen (PCNA) loading onto DNA (PCNA(DNA)) triggers the interact

57 -induced proliferating cell nuclear antigen (PCNA) monoubiquitination in Poleta-proficient but not in

58 support proliferation cell nuclear antigen (PCNA) monoubiquitination.

59 CDK) and proliferating cell nuclear antigen (PCNA) onto chromatin, as well as initiation and elongati

60 ng clamp proliferating cell nuclear antigen (PCNA) plays a vital role in a number of DNA repair pathw

61 ECQ5 and proliferating cell nuclear antigen (PCNA) promotes RAD18-dependent PCNA ubiquitination and t

62 s to the proliferating cell nuclear antigen (PCNA) sliding clamp to form a holoenzyme.

63 protein proliferating cell nuclear antigen (PCNA) to DNA lesions.

64 cts with proliferating cell nuclear antigen (PCNA) via a highly conserved PIP box motif within the ki

65 itinated Proliferating Cell Nuclear Antigen (PCNA), a marker of stalled replication forks, interacts

66 act with proliferating cell nuclear antigen (PCNA), an essential co-factor for DNA polymerases in bot

67 f Cdc45, proliferating cell nuclear antigen (PCNA), and polymerase delta, but not ORC and MCM protein

68 f Mcl-1, proliferating cell nuclear antigen (PCNA), and pro-caspase-3.

69 2-Msh3), proliferating cell nuclear antigen (PCNA), and replication factor C (RFC) and a reconstitute

70 on clamp proliferating cell nuclear antigen (PCNA), respectively.

71 tment of proliferating cell nuclear antigen (PCNA), the platform for assembly of the DNA replication

72 Proliferating cell nuclear antigen (PCNA), the processivity factor for DNA replication, play

73 but not proliferating cell nuclear antigen (PCNA)-interacting motif (PIM), leads to increased cell d

74 the Elg1 proliferating cell nuclear antigen (PCNA)-unloader complex.

75 ption of proliferating cell nuclear antigen (PCNA).

76 ion with proliferating cell nuclear antigen (PCNA).

77 TDG with proliferating cell nuclear antigen (PCNA).

78 n of the proliferating cell nuclear antigen (PCNA).

79 eric proliferating cellular nuclear antigen (PCNA).

80 pha, and proliferating cell nuclear antigen (PCNA).

81 itors of proliferating cell nuclear antigen (PCNA)/PCNA interacting protein box (PIP-Box) interaction

82 such as proliferating cell nuclear antigen (PCNA); however, the exact mechanism of PCNA activation i

83 Our results provide evidence for an archaeal PCNA 'tool-belt' recruitment model of multienzyme functi

84 DNA polymerase eta, have two architectures: PCNA tool belts and Rev1 bridges.

85 regulated sites on known DDR players such as PCNA and identify previously unknown DDR targets such as

86 MutLalpha interaction with PCNA, as well as PCNA-dependent activation of MutLalpha endonuclease, PCN

87 o an increased level of chromatin-associated PCNA.

88 d a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activit

89 pa and the PIP motif of yeast Msh6 bind both PCNA and Rev1.

90 translesion synthesis polymerase, binds both PCNA and Rev1.

91 ing specificities and can interact with both PCNA and Rev1 in structurally similar ways.

92 ng eukaryotic replication is orchestrated by PCNA ubiquitination.

93 ction pathway is apparently not regulated by PCNA and 14-3-3s.

94 LS polymerases, PrimPol is not stimulated by PCNA and does not interact with it in vivo.

95 that Elg1 unloads the DNA replication clamp PCNA from DNA, we tested whether PCNA overexpression wou

96 adaptive mutations in the replication clamp PCNA were introduced.

97 it, Mcm4, and the replication sliding clamp, PCNA, between different stages of the cell cycle and bet

98 cal marker where interaction with beta-clamp/PCNA could distinguish parent/daughter strand identity.

99 f MutL's endonuclease activity by beta-clamp/PCNA remains elusive.

100 with the DNA replication machinery component PCNA and promotes replication of DNA lesions and common

101 Thus, WRNIP1 connects PCNA monoubiquitination with ATMIN/ATM to activate ATM s

102 directly interacts with PCNA via a conserved PCNA-interacting peptide (PIP) box motif.

103 ctions are mediated by one or more conserved PCNA-interacting protein (PIP) motifs that bind in a hyd

104 T2AA decreased PCNA/pol eta and PCNA/REV1 chromatin colocalization but

105 We further define PCNA as a key regulator of ZRANB3 function, which recrui

106 leting replication, and we propose Pol delta-PCNA collides with the slower CMG, and in the absence of

107 However, Saccharomyces cerevisiae Pol delta-PCNA is a rapid and processive enzyme, suggesting that C

108 We have shown earlier that Pol delta-PCNA is suppressed on the leading strand with CMG.

109 Conversely, Pol delta-PCNA is the only enzyme capable of extending Okazaki fra

110 Msh6 (or Msh2-Msh3), Exo1, RPA, RFC-Delta1N, PCNA, and Pol epsilon was found to catalyze an MMR react

111 Msh6 (or Msh2-Msh3), Exo1, RPA, RFC-Delta1N, PCNA, and Pol epsilon was found to catalyze both short-p

112 lear antigen (PCNA) promotes RAD18-dependent PCNA ubiquitination and the helicase activity of RECQ5 p

113 ell nuclear antigen (PCNA) loading onto DNA (PCNA(DNA)) triggers the interaction between CRL4(CDT2) a

114 sociation and formation of a 1:1:1 NEIL1-DNA-PCNA(monomer) complex.

115 1 by Srs2 helicase is required for efficient PCNA loading and restoration of resected DNA As a result

116 endent activation of MutLalpha endonuclease, PCNA- and DNA-dependent activation of MutLalpha ATPase,

117 in the number of principal cells expressing PCNA in the papilla.

118 thesis together with its processivity factor PCNA.

119 els of Pdx-1 (insulin transcription factor), PCNA (a marker of cell proliferation), and LC3 (a marker

120 reduced alphaSMA immunoreactivity and fewer PCNA (+) nuclei among alphaSMA (+) cells (P < 0.008).

121 played decreased alphaSMA staining and fewer PCNA (+) nuclei in VSMC (P < 0.005).

122 Cdt1 even though p21 has higher affinity for PCNA(DNA).

123 amatically transforms the binding pocket for PCNA client proteins.

124 RAD51, but not BRCA2, is also required for PCNA monoubiquitination in response to HU, suggesting th

125 portant for ATP binding is also required for PCNA poly-ubiquitination and recombination-based lesion

126 IP1 and RAD18, the E3 ligase responsible for PCNA monoubiquitination, are specifically required for A

127 These results reveal a central role for PCNA in the Exo1-independent MMR pathway and suggest tha

128 s are needed to determine possible roles for PCNA and other host proteins detected.IMPORTANCE Poxviru

129 icament lies ahead for the replication fork, PCNA is there to orchestrate the events necessary to han

130 itive than XPA in binding replication forks, PCNA sequestration by progerin may shift the equilibrium

131 tes the rapid dissociation of pol delta from PCNA on stalling at a DNA lesion.

132 CT116 (HCT116-OxR) cells and that gammaH2AX, PCNA, and FANCD2 monoubiquitinations are induced by oxal

133 We show here that human PCNA and MutLalpha interact specifically but weakly in s

134 and NMR studies, has revealed how the human PCNA clamp slides on DNA.

135 ngle point mutation, Ser228Ile, in the human PCNA gene was recently identified to cause a disease who

136 Here we identify PCNA-associated factor (PAF) as a key molecule that cont

137 lymphocytes by double immunohistochemistry (PCNA-staining) and flow cytometry (BrdU incorporation) r

138 ation of Thr(6) or Tyr(8) on UNG2 can impede PCNA binding without affecting UNG2 catalytic activity o

139 r mutation of Tyr-60, Tyr-133, or Tyr-250 in PCNA abrogated its ubiquitination.

140 icated tyrosine residues 60, 133, and 250 in PCNA as IGF-1R targets, and PCNA phosphorylation was fol

141 en combined with Cac1 mutations deficient in PCNA binding.

142 further support a role for Bub1 and LANA in PCNA-mediated cellular DNA replication processes as well

143 Due to this fundamental role, mutations in PCNA that profoundly impair protein function would be in

144 tomatically classifying cell cycle phases in PCNA-immunolabeled cells from single time point images,

145 t FANCD2 and RAD51 have an important role in PCNA monoubiquitination and TLS in a FANCD2 monoubiquiti

146 ns for the plasticity of the binding site in PCNA and reveals how a disease mutation selectively alte

147 findings reveal a modulatory role of USP7 in PCNA ubiquitination-mediated stress-tolerance pathways b

148 estabilizes Rad18 and compromises UV-induced PCNA mono-ubiquitylation and Pol eta recruitment to stal

149 suggesting that USP7 facilitates UV-induced PCNA monoubiquitination by stabilizing Poleta.

150 Rad5 enzymatic domains concertedly influence PCNA modification, and unveil their discrete contributio

151 chromatin colocalization but did not inhibit PCNA monoubiquitination, suggesting that T2AA hinders in

152 tein, called TIP, binds to PCNA and inhibits PCNA-dependent activities although it does not contain a

153 with the Elg1 complex and down-regulate its PCNA-unloading function to promote the G1/S transition.

154 sites (Thr(6) and Tyr(8)) located within its PCNA-interacting motif (PIP-box).

155 r ptDNA as the correct substrate for loading PCNA.

156 pathway and suggest that Msh2-Msh6 localizes PCNA to repair sites after mispair recognition to activa

157 marker (BLBP) and cell proliferation marker (PCNA).

158 tering analysis confirmed the NEIL1 mediated PCNA trimer dissociation and formation of a 1:1:1 NEIL1-

159 The Rad5 ubiquitin ligase activity mediates PCNA poly-ubiquitination and subsequently recombination-

160 nd various post-translational modifications, PCNA has far-reaching impacts on a myriad of cellular fu

161 in cell cycle regulation through modulating PCNA levels on chromatin.

162 er show that gammaH2AX and monoubiquitinated PCNA and FANCD2 are constitutively up-regulated in oxali

163 hesis that is regulated by monoubiquitinated PCNA.

164 ared to unmodified PCNA or monoubiquitinated PCNA.

165 s of pol eta and REV1 with monoubiquitinated PCNA.

166 mutation lies near the binding site for most PCNA-interacting proteins.

167 A, with or without Msh2-Msh6 (or Msh2-Msh3), PCNA, and RFC but did not require nicking of the substra

168 NBN1, PCNA (proliferating nuclear antigen), GADD45A (DNA damag

169 ification and functional analysis of a novel PCNA interacting protein NreA that is conserved in the a

170 UVA laser, H2O2, and at sites of sub-nuclear PCNA foci, suggesting that poly (ADP-ribose) promotes XR

171 models that are compatible with the observed PCNA recruitment data if FRAP is not used.

172 t strand displacement even in the absence of PCNA.

173 activity was clearly enhanced by addition of PCNA in vitro.

174 In vitro MS analysis of PCNA co-incubated with the IGF-1R kinase indicated tyros

175 also been shown to block the association of PCNA with chromatin.

176 siRNA-mediated depletion of PCNA or components of CRL4(Cdt2), specifically cullin4A/

177 tative RT-PCR to determine the expression of PCNA and Bax/Bcl-2.

178 induced caspase 3 activity and expression of PCNA and Ki67, but activation of the Akt survival pathwa

179 e IL-33-positive cells had low expression of PCNA.

180 The formation of PCNA tool belts and Rev1 bridges and the ability of thes

181 DNA Polymerase delta with different forms of PCNA.

182 o the subunit interface of the homotrimer of PCNA in addition to the PIP-box binding cavity.

183 Single time point images of PCNA-immunolabeled cells are acquired using confocal and

184 and the ensuing TLS are both independent of PCNA ubiquitination.

185 Independently of PCNA binding, Srs2 also displaces Rad51 from nascent str

186 critical to the cancer-associated isoform of PCNA.

187 f Enok reduced the chromatin-bound levels of PCNA in both S2 cells and early embryos, suggesting that

188 esults indeed reveal that elevated levels of PCNA rescue pds5-1 temperature sensitivity and cohesion

189 ion within the interdomain connector loop of PCNA, and much of the regulation is a result of the inhe

190 forks, concurrent with a significant loss of PCNA at the forks, whereas PCNA efficiently bound to pro

191 igen (PCNA); however, the exact mechanism of PCNA activation is currently unknown.

192 ides a structural basis for the mechanism of PCNA inhibition by TIP.

193 eview, we focus on the monoubiquitination of PCNA by Rad6/Rad18 and summarize the current knowledge o

194 n processes as well as monoubiquitination of PCNA in response to UV damage.

195 Together, our findings detail a mutation of PCNA in humans associated with a neurodegenerative pheno

196 PRP- BMA presented higher numbers of PCNA-positive and BSP-positive cells than control at 10

197 ase) based on the characteristic patterns of PCNA distribution, is feasible for both confocal and wid

198 ase) based on the characteristic patterns of PCNA distribution.

199 show that c-Abl and Y211 phosphorylation of PCNA is an important axis downstream of Ron, which is re

200 If this target region of PCNA is modified, the DNA replication and repair process

201 Progerin sequestration of PCNA promotes replication fork collapse and mislocalizat

202 in a hydrophobic pocket on the front side of PCNA as well as by conserved Rev1-interacting region (RI

203 motif of EndoQ and the toroidal structure of PCNA are critical for the stimulation of the endonucleas

204 The crystal structure of PCNA in complex with T2AA revealed that T2AA bound to th

205 The trimeric structure of PCNA results in slow subunit association rates and is la

206 lly, we present the co-crystal structures of PCNA with two specific motifs in ZRANB3: the PIP box and

207 n of lysine residues at the inner surface of PCNA is induced by DNA lesions.

208 are activated by the ubiquitylation (ub) of PCNA through components of the RAD6-RAD18 pathway, where

209 rgistically triggered mono-ubiquitination of PCNA and apoptosis in a RAD18-dependent manner.

210 roximately 1:1 stoichiometry that depends on PCNA interaction with the C-terminal endonuclease domain

211 kinase C delta are both required for optimal PCNA expression.

212 clamp, proliferating cell nuclear antigen or PCNA, is a ring-shaped protein complex that surrounds DN

213 plication processivity factor (beta-clamp or PCNA) activate the latent MutL endonuclease to nick the

214 of proliferating cell nuclear antigen (PCNA)/PCNA interacting protein box (PIP-Box) interactions, inc

215 discovered that, beginning in late S phase, PCNA(DNA) is no longer sufficient to trigger CRL4(CDT2)-

216 hat IGF-1R interacts with and phosphorylates PCNA in human embryonic stem cells and other cell lines.

217 ivity, which in turn directly phosphorylates PCNA at Y211 and leads to an increased level of chromati

218 via a short peptide sequence known as a PIP (PCNA interacting protein) motif.

219 enhancing the processivity of the polymerase PCNA is an allosteric modulator of other Pol delta activ

220 case ZRANB3, shown to bind polyubiquitinated PCNA.

221 y and its interaction with polyubiquitinated PCNA, pinpointing ZRANB3 as a key effector of error-free

222 tin ligase RAD18 and the replication protein PCNA.

223 es (PARP1, MSH2, Ku, DNA-PKcs, MCM proteins, PCNA and DNA Pol delta) and in protein metabolic process

224 roper balance between the anti-recombination PCNA-bound and pro-recombination pools of Srs2 is crucia

225 LANA recruited PCNA to the KSHV genome via Bub1 to initiate viral repli

226 Thus, acetylation regulates PCNA sliding on DNA in the presence of DNA damage, favor

227 ed fork reversal in mammalian cells requires PCNA ubiquitination, UBC13, and K63-linked polyubiquitin

228 XPA or progerin each significantly restored PCNA at replication forks.

229 1 (Exo1), replication protein A (RPA), RFC, PCNA, and DNA polymerase delta.

230 -induced telomere synthesis requires the RFC-PCNA-Pol delta axis, but is independent of other canonic

231 The p21's PCNA interacting region (PIR), and not its CDK binding d

232 The approach is applied to seven PCNA x-ray crystallographic data sets with resolutions 2

233 sis from Finasteride treated patients showed PCNA expression in BECs was highly correlated to the lev

234 TLS and TS depend on site-specific PCNA K164 monoubiquitination and polyubiquitination, res

235 In addition to specific PCNA and polymerase interactions (PIP site), we have now

236 inctive doughnut-shaped molecular structure, PCNA was originally studied for its role in stimulating

237 d mono-ubiquitination of the RAD18 substrate PCNA is attenuated by MAGE-A4 silencing.

238 tin-conjugating enzyme UBE2D3 with substrate PCNA), and endogenous proteins interacting with thioredo

239 ; it not only mediates interaction with SUMO-PCNA to promote the anti-recombination function but it a

240 oops over unextended D-loops when SUMOylated PCNA is present, compared to unmodified PCNA or monoubiq

241 s the inhibition of ribosomal RNA synthesis, PCNA expression, and T-cell activation induced by MPA, s

242 s may serve as a flexible scaffold to tether PCNA and RPA at the replication fork, and that post-tran

243 Stokes radii measured by SEC hinted that PCNA in complex with NEIL1 (+/-DNA) was no longer a trim

244 However, we now know that PCNA does much more than promote processive DNA synthesi

245 We propose that PCNA and ATP-dependency serve as a multi-layered regulat

246 s, in vitro and in vivo analysis showed that PCNA unloading is delayed in the absence of nucleosome a

247 nines relay critical information between the PCNA-binding, DNA-binding, and ATPase sites at all steps

248 In humans, the PCNA-associated recombination inhibitor (PARI) protein h

249 Mutations in the PCNA-interacting peptide (PIP) motif of TDG that disrupt

250 ovide important structural insights into the PCNA-APIM interaction, and reveal unexpected similaritie

251 on DNA polymerase, the RFC clamp loader, the PCNA sliding clamp, and the RPA single-stranded DNA bind

252 Because of the heterotrimeric nature of the PCNA clamp in some archaea, there is potential to occupy

253 py also demonstrated the dissociation of the PCNA homotrimer in the presence of NEIL1 and DNA, while

254 gets lysine 20 at the sliding surface of the PCNA ring in vitro and in vivo in response to DNA damage

255 cations function at the outer surface of the PCNA ring to favor DNA damage bypass.

256 tering (SAXS) confirms the disruption of the PCNA trimer upon addition of the TIP protein in solution

257 en protomers in the crystal structure of the PCNA-K20ac ring.

258 the UNG2 N-terminus disrupt formation of the PCNA-UNG2-RPA protein complex.

259 nrecognized contribution of the motif to the PCNA and ubiquitination enzyme interaction, and not due

260 lated by the conjugation of ubiquitin to the PCNA sliding clamp by distinct E2/E3 pairs.

261 tionship between domains I and II within the PCNA monomer such that the trimeric ring structure is br

262 This PCNA sequestration likely exposed ds-ssDNA junctions at

263 This PCNA-related trimer is loaded onto RPA-coated single str

264 All proteins that bind to PCNA do so via a PCNA-interacting peptide (PIP) motif th

265 Importantly, we show that PARP10 binding to PCNA is required for translesion DNA synthesis.

266 A small protein, called TIP, binds to PCNA and inhibits PCNA-dependent activities although it

267 ly replication polymerase (YB site) bound to PCNA and DNA from Sulfolobus solfataricus.

268 The X-ray crystal structure of TIP bound to PCNA reveals that TIP binds to the canonical PIP interac

269 tive evidence that the binding of pol eta to PCNA and the ensuing TLS are both independent of PCNA ub

270 w that in the absence of Srs2 recruitment to PCNA or in helicase-deficient mutants, breakage at a CAG

271 TLS involves the conjugation of ubiquitin to PCNA clamps encircling damaged DNA and the role of this

272 y the alcohol, coupled to a proton transfer (PCNA: proton-coupled nucleophilic attack) and a subseque

273 e variant bound to peptides derived from two PCNA partner proteins reveal that the binding pocket can

274 y-accepted model purports that ubiquitinated PCNA recruits TLS polymerases such as pol eta to sites o

275 e observed increased levels of ubiquitylated PCNA and significantly lower mutation frequency in the t

276 domain, helps recruit FAN1 to ubiquitylated PCNA accumulated at stalled forks.

277 ities and its interaction with ubiquitylated PCNA may offer therapeutic opportunities for treatment o

278 t FAN1 contains a previously-uncharacterized PCNA interacting peptide (PIP) motif that, together with

279 Our data also suggests that unmodified UNG2, PCNA, and RPA can form a ternary protein complex.

280 ated PCNA is present, compared to unmodified PCNA or monoubiquitinated PCNA.

281 gnificant loss of PCNA at the forks, whereas PCNA efficiently bound to progerin.

282 ation clamp PCNA from DNA, we tested whether PCNA overexpression would similarly rescue pds5-1 mutant

283 pol delta maintains a loose association with PCNA while replicating DNA.

284 in part, by suppressing its association with PCNA.

285 rase eta mediates its interactions both with PCNA and with Rev1.

286 polymerase Poleta that also colocalized with PCNA.

287 how that LANA is able to form a complex with PCNA, a critical protein for viral DNA replication.

288 Interaction of Pol delta with PCNA eliminates flap-mediated inhibition of strand displ

289 to a compensating interaction of DNMT1 with PCNA.

290 Many of these proteins interact with PCNA via a short peptide sequence known as a PIP (PCNA i

291 e SIRV2 proteins were found to interact with PCNA, providing insights into the recruitment of host re

292 l replication in S phase and interacted with PCNA to promote its monoubiquitination in response to UV

293 We conclude that Srs2 interaction with PCNA allows the helicase activity to unwind fork-blockin

294 in the nucleus enhanced its interaction with PCNA in squamous cell carcinoma of the head and neck (SC

295 or abolish human MutLalpha interaction with PCNA, as well as PCNA-dependent activation of MutLalpha

296 hat disrupted the Msh2-Msh6 interaction with PCNA.

297 plication forks that directly interacts with PCNA via a conserved PCNA-interacting peptide (PIP) box

298 rase carries out processive replication with PCNA in vitro; however, in yeast, it requires an increas

299 TDG that disrupt the interaction of TDG with PCNA or change critical basic residues essential for the

300 ays to explore the interactions of UNG2 with PCNA and RPA and to determine the effects of two UNG2 ph