ライフサイエンスコーパス: PCR amplification

1 gene was confirmed by failure of 3'- and 5'-PCR amplification.

2 ve patients were also positive for H. pylori PCR amplification.

3 PCR chambers enables real-time monitoring of PCR amplification.

4 f the formation of chimeric sequences during PCR amplification.

5 s to heat the sample and perform a real-time PCR amplification.

6 at low copy numbers intrinsic to exponential PCR amplification.

7 s compatible with downstream microchip-based PCR amplification.

8 of purifying both RNA and DNA for downstream PCR amplification.

9 horesed to a recovery chamber for subsequent PCR amplification.

10 s genotyping accuracy and removes noise from PCR amplification.

11 or dynamic solid phase extraction (dSPE) and PCR amplification.

12 identical length can also be generated using PCR amplification.

13 The s type and the m type were determined by PCR amplification.

14 strand displacement amplification (SDA), and PCR amplification.

15 mited by the extraction procedure and not by PCR amplification.

16 3 days prior to DNA extraction and real-time PCR amplification.

17 tides and that operates without the need for PCR amplification.

18 sulting in rapid (55-s cycles) and efficient PCR amplification.

19 es we show that the latter are not skewed by PCR amplification.

20 ime of 1.5 h and without the need for sample PCR amplification.

21 croarray following reverse transcription and PCR amplification.

22 t the lower temperatures that exist prior to PCR amplification.

23 the limitations associated with conventional PCR amplification.

24 iosynthesis; it can be completed 45 min post-PCR amplification.

25 olecule, allowing real-time detection during PCR amplification.

26 pendent experiments and by performing direct PCR amplification.

27 f target BWAs in spiked sewage samples after PCR amplification.

28 o a 550-nl chamber for rapid target sequence PCR amplification.

29 ational polymorphism analysis after specific PCR amplification.

30 r ( approximately 50,000 cells) was used for PCR amplification.

31 single molecules and real-time quantitative PCR amplification.

32 in dogs, based upon isolation, serology, or PCR amplification.

33 ct the exponential and linear phases of LATE-PCR amplification.

34 tic processes like reverse transcription and PCR amplification.

35 ause of the stochastic nature of exponential PCR amplification.

36 large quantities of marked DNA to be made by PCR amplification.

37 extracted from bacterial culture and without PCR amplification.

38 artifacts such as template switching during PCR amplification.

39 cific mtDNA from wastewater before and after PCR amplification.

40 s without the need for long culture steps or PCR amplification.

41 nts suffer from distortions occurring during PCR amplification.

42 e amplification of wild type DNA during LATE-PCR amplification.

43 o the start of the exponential phase of LATE-PCR amplification.

44 challenges for studies based on conventional PCR amplification.

45 roximately 36 molecules per sample), without PCR amplification.

46 ic microorganisms and eliminated the need of PCR amplifications.

47 of extraction and polymerase chain reaction (PCR) amplification.

48 l (TRAP) based on polymerase chain reaction (PCR) amplification.

49 typically employ polymerase chain reaction (PCR) amplification after bisulfite conversion, thereby l

50 PCR amplification, agarose gel electrophoresis, and sequ

51 The ability to subtype genomic DNA without PCR amplification allows probes to be designed for many

52 tive transcription factors were generated by PCR amplification and assessed for transcriptional activ

53 These ESTs allow for specific PCR amplification and broad coverage across the genome,

54 tion and an m2000rt instrument for real-time PCR amplification and detection.

55 PCR amplification and direct sequencing of portions of t

56 a(KPC) genes and was combined with selective PCR amplification and DNA sequencing for complete charac

57 ary construction, cloned sequence isolation, PCR amplification and DNA sequencing of cloned inserts f

58 The i type was identified by PCR amplification and DNA sequencing.

59 A challenging target sequence for both PCR amplification and electrochemical detection allowed

60 ID system is a novel technology that couples PCR amplification and electrospray ionization-mass spect

61 PCR amplification and enzyme digestions were used to ana

62 rat TERT (rTERT) gene promoter was cloned by PCR amplification and fused with a luciferase reporter g

63 The combined use of emulsion PCR amplification and high-throughput sequencing allows

64 PCR amplification and high-throughput sequencing theoret

65 tion with a bridge linker, Tn5 tagmentation, PCR amplification and high-throughput sequencing.

66 Masking DNA lacks primer regions for PCR amplification and is typically taken in excess to th

67 A system that automatically performs the PCR amplification and microchip electrophoretic (ME) sep

68 nomic DNA from bacteria without the need for PCR amplification and molecular labeling is described.

69 We performed PCR amplification and next generation deep immune repert

70 DNA synthesis (TLS) polymerase, followed by PCR amplification and next-generation sequencing (NGS) t

71 hes to molecular diagnostics rely heavily on PCR amplification and optical detection methods which ha

72 can be undertaken in less than 1 h following PCR amplification and permits identification of species

73 adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserv

74 PCR amplification and pyrosequencing was carried out to

75 Reverse transcription-PCR amplification and RACE were used to acquire the form

76 Reverse transcription-PCR amplification and RACE were used to acquire the rema

77 we demonstrate PCR-chimera formation during PCR amplification and reference alignment bias are pitfa

78 Genomic PCR amplification and resequencing indicated that the ge

79 ongenital infection was determined by direct PCR amplification and restriction fragment length polymo

80 Also, based on PCR amplification and sequence analysis of the DNA joint

81 PCR amplification and sequence analysis of the dtxR gene

82 e present here an analysis of the utility of PCR amplification and sequence analysis of the hypervari

83 % of the Acidobacteria Group 1 sequences, by PCR amplification and sequencing of DNA from soil.

84 al masses, previously formally identified by PCR amplification and sequencing of internal transcribed

85 ction of Pathogenic Fungi were identified by PCR amplification and sequencing of internal transcribed

86 We used PCR amplification and sequencing of retroviral fragments

87 Helicobacter species were characterized by PCR amplification and sequencing of the 16S rRNA, urease

88 2 isolates, and bla(KPC-3) was identified by PCR amplification and sequencing of the amplicon.

89 biochemical tests (AUXACOLOR2) and following PCR amplification and sequencing of the D1-D2 portion of

90 Further, we show that PCR amplification and sequencing of the D1/D2 region rel

91 PCR amplification and sequencing of the D1D2 and interna

92 r the MICs of cefpodoxime were >4 microg/ml, PCR amplification and sequencing of the ESBL and pAmpC g

93 PCR amplification and sequencing of the most variable N-

94 ication compared with ribosomal markers, low PCR amplification and sequencing success eliminated them

95 a null allele (Lim2(Gt)), as verified by RT-PCR amplification and sequencing, RNA blotting, immunobl

96 ified from the DMNQ-resistant VSMC clones by PCR amplification and sequencing.

97 PCR amplification and serological testing identified the

98 suitable for downstream applications such as PCR amplification and shotgun sequencing.

99 The use of more than 20 cycles of PCR amplification and/or more than 50 ng of starting gen

100 By RT-PCR amplifications and DNA sequencing, numerous in-frame

101 owever, biases in polymerase chain reaction (PCR) amplification and in cloning can skew the results.

102 ional strategy of polymerase chain reaction (PCR) amplification and sequencing of individual exons.

103 g unmethylated Cs to Us and then to Ts after PCR amplification) and next generation sequencing (NGS)

104 ecules of miRNA per 30 muL of sample without PCR amplification, and can be operated within the dynami

105 beta-joining(J)beta fingerprint generated by PCR amplification, and determined the primary structure

106 DNA extraction, PCR amplification, and direct sequencing of the COL8A1 a

107 DNA extraction, PCR amplification, and direct sequencing of the COL8A2,

108 DNA extraction, PCR amplification, and direct sequencing of the VSX1 gen

109 abrication of microchips for DNA extraction, PCR amplification, and DNA fragment separation, includin

110 omplete assay, including sample preparation, PCR amplification, and electrochemical detection of Camp

111 oratory steps, DNA quantitation and pooling, PCR amplification, and electrophoresis, accounted for 23

112 tandard Sanger sequencing, genotype-specific PCR amplification, and non-HCV-specific Illumina RNA seq

113 g to errors in sampling, random mutations in PCR amplification, and probably mostly variations in rea

114 xtraction procedures, reverse transcription, PCR amplification, and real-time detection at target con

115 r sample preparation, reverse transcription, PCR amplification, and sequencing.

116 rimers were used for nonspecific (universal) PCR amplification, and serotype-specific probes coupled

117 rom bacterial ribosomes without the need for PCR amplification, and single DNA strands produced from

118 mber of templates are used in initiating the PCR amplification, and this can lead to unrecognized seq

119 nucleotide sequence analysis using a nested PCR amplification approach for each gene.

120 ed in 50% examined transgenic cotton through PCR amplification assay and sequencing analyses.

121 CR (smPCR) amplification would eliminate the PCR amplification bias because competition between templ

122 w per-base sequencing quality at the 3' end, PCR amplification bias, and bisulfite conversion rates.

123 ng and detect cross-sample contamination and PCR amplification bias.

124 ved from metabarcoding due to taxon specific PCR amplification biases.

125 After the subsequent bisulfite treatment and PCR amplification, both cytosine and 5-caC (derived from

126 sequences among Bartonella species, a single PCR amplification can be used to detect different specie

127 RT-PCR amplification, cloning, and sequencing of the monocl

128 ed from small water samples using wide-range PCR amplification combined with high-throughput sequenci

129 Real-time RT-PCR amplification confirmed the presence of specific gen

130 Fluorescent output of multiplexed PCR amplification could also be imaged with the cell pho

131 However, in vitro recombination during PCR amplification could not be excluded.

132 PCR amplification coupled with electrospray ionization m

133 s and analyzes the samples using multiplexed PCR amplification coupled with microsphere array detecti

134 g multiplexed reverse transcription-PCR (mRT-PCR) amplification coupled with a microsphere hybridizat

135 through Poisson statistics, showed real-time PCR amplification curves with a cycle threshold of appro

136 cts generated from 15, 27, 35, and 40 EDS-CF-PCR amplification cycles were collected and analyzed usi

137 e developed probes that, in combination with PCR amplification, detect low concentrations of variant

138 tudy, the use of the Endo IV assay as a post-PCR amplification detection system is demonstrated.

139 After RT-PCR amplification, DNAs amplified from bunyaviruses of i

140 lfite-modified genomic DNA to a second-round PCR amplification employing GC-tagged primers.

141 After PCR amplification, end-point detection is achieved using

142 PCR amplification experiments after overnight growth wit

143 We also examined two strategies for PCR amplification-flanking PCR, which uses primers that

144 enables this information to be recovered by PCR amplification followed by DNA sequencing.

145 Instead, PCR amplification followed by heteroduplex scanning and/

146 ducts), which uses target-enriched multiplex PCR amplification followed by liquid array identificatio

147 e-genome sequencing (WGS) strategy, based on PCR amplification followed by next-generation sequencing

148 th; this is presumably caused by inefficient PCR amplification for long fragments.

149 In addition, PCR amplification formulations were optimized to tolerat

150 After limited PCR amplification, fragments are sequenced using a high-

151 used by A. terreus and A. niger, culture and PCR amplification from BAL fluid yielded similar sensiti

152 RT-PCR amplification from human hippocampal mRNA confirmed

153 dge that included both sample processing and PCR amplification functions was loaded with all reagents

154 ELP data analysis is complicated not only by PCR amplification heterogeneity but also by a complex an

155 and instrumentation to accomplish universal PCR amplification, High Resolution Melting (HRM), and ma

156 here longer fragments are more available for PCR amplification in air dried material compared to alco

157 DNA extraction or polymerase chain reaction (PCR) amplification in just 75 min.

158 PCR amplification introduces redundant reads in the sequ

159 PCR amplification is an important step in the preparatio

160 sequence polymorphism in Pacific oysters, Q-PCR amplification is sub-optimal in some individuals bec

161 A major influence on the PCR amplification is the size of the restriction fragmen

162 rectly extracted from lymphocytes (bypassing PCR amplification), is reported.

163 A sequence-independent PCR amplification method was used to identify viral nucl

164 pproach with those of more-conventional bulk PCR amplification methods performed on the same patient

165 solution despite sequence-dependent bias and PCR-amplification noise, and is analogous to digital PCR

166 PCR amplification of 16S rRNA genes with universal prime

167 Applied to the PCR amplification of 47,000-60,000-year-old cave bear DN

168 as indicated by the subsequent chip-based IR-PCR amplification of a 211-bp fragment of the B. anthrac

169 irst method, the isolates were studied using PCR amplification of a fragment of the C. parapsilosis s

170 = 3), and the purified DNA was suitable for PCR amplification of a fragment of the gelsolin gene.

171 quence of the native chicken liver enzyme by PCR amplification of a series of 72 overlapping primers.

172 Although assays utilizing PCR amplification of bisulfite-converted DNA are widely

173 conditions and increased sensitivity in the PCR amplification of bisulfite-treated DNA.

174 PCR amplification of bla genes revealed that one-third (

175 template for DNA polymerization, that allows PCR amplification of boronic acid-labeled DNA.

176 In addition, simultaneous real-time PCR amplification of both multiple different samples and

177 quence-independent reverse transcription and PCR amplification of capsid-protected, nuclease-resistan

178 the Dpo4-like enzymes ideally suited for the PCR amplification of damaged DNA samples.

179 Here, we examine the PCR amplification of DNA containing one or more d5SICS-d

180 tified as Enterococcus hirae on the basis of PCR amplification of DNA extracted from the formalin-fix

181 Typically, PCR amplification of DNA from each panel cell line is fo

182 PCR amplification of DNA is a key preliminary step in ma

183 ase has served as the stalwart enzyme in the PCR amplification of DNA.

184 hanisms involved in chimera formation during PCR amplification of environmentally derived DNA.

185 to those of natural isolates sequenced by RT-PCR amplification of field samples.

186 perature and can be used as the template for PCR amplification of fragments of at least 8 kb.

187 Brain inflammation was measured by TaqMan RT-PCR amplification of genes known to be up-regulated in r

188 PCR amplification of genomic DNA and sequencing of the g

189 Here, we describe a method for PCR amplification of lesion-containing DNA in which the

190 The C(T) values for PCR amplification of lysed samples using primers specifi

191 , in the inoculated models, was confirmed by PCR amplification of M13 minisatellite.

192 We report evidence of nonspecific PCR amplification of Mesorhizobium species with previous

193 In the DNA-based method, PCR amplification of mitochondrial D loop gene using spe

194 The PCR amplification of oligonucleotides enables the evolut

195 g sequence is labeled with a unique OMT, and PCR amplification of OMTs is performed after removing no

196 printing method that relies on the selective PCR amplification of restriction fragments.

197 ns on streptavidin-coated magnetic beads and PCR amplification of single-cell Hi-C libraries.

198 fluorescence-labeled oligonucleotide probes, PCR amplification of species-specific gene sequences, an

199 where conventional thermocycling allowed for PCR amplification of specific DNA target sequences.

200 ay is a rapid, semiautomated method based on PCR amplification of specific regions between noncoding

201 Traditional STR analyses were based on the PCR amplification of STR loci followed by gel electropho

202 Using solely a gene-based procedure, PCR amplification of the 16S ribosomal RNA gene coupled

203 ps: pooled growth, isolation of genomic DNA, PCR amplification of the barcodes, array hybridization a

204 with sodium bisulphite, usually followed by PCR amplification of the chosen target region.

205 on through enzymatic ligation; and (iii) qRT-PCR amplification of the circularized PLP after removal

206 samples by Ms-SNuPE in less than 5 h, after PCR amplification of the desired target sequence and pre

207 PCR amplification of the GAPDH gene was demonstrated at

208 ently, our laboratory relied on conventional PCR amplification of the internal transcribed spacer 2 (

209 liquid-based cytology medium using real-time PCR amplification of the L1 gene and TaqMan probes.

210 Genotyping was performed after PCR amplification of the region encompassing the polymor

211 ; ligation of an adapter to the genomic DNA; PCR amplification of the regions flanking the T-DNA inse

212 erse transcription of the associated RNA and PCR amplification of the resultant cDNA with gene-specif

213 be distinguished from those arising through PCR amplification of the same molecule.

214 ligation of an adapter to the genomic DNA; a PCR amplification of the T-DNA/genomic DNA junction with

215 results were compared to those of ELISA and PCR amplification of the toxin genes.

216 ted from the plaque samples and subjected to PCR amplification of the V1-V3 region of the 16S rDNA.

217 cell RNA, enabling reverse transcription and PCR amplification of these sequences.

218 PCR amplification of three unrelated genes resulted in c

219 shearing of genomic DNA followed by specific PCR amplification of transposon-containing fragments and

220 ird CDR of the H chain intervals obtained by PCR amplification of V(H) domain DNA from DEX-specific p

221 tive immune receptor repertoires depend upon PCR amplification of VDJ rearrangements followed by long

222 the experimental conditions are suitable for PCR amplification of VEGF-A165b mRNA for both q-PCR and

223 ese determinations were made by either using PCR amplification of viral transcripts in bulk cell popu

224 Utilizing PCR amplifications of genes from these loci, we were abl

225 man GLTP gene, in silico EST evaluations, RT-PCR amplifications of GLTP transcript(s), and methylatio

226 on of broad-range polymerase-chain-reaction (PCR) amplification of 16S rDNA with clone analysis, bact

227 e progress of DNA polymerase chain reaction (PCR) amplification of a minor component of a DNA extract

228 opic observation, polymerase chain reaction (PCR) amplification of blood samples, and serological tes

229 id was extracted, polymerase chain reaction (PCR) amplification of desmosome-encoding genes was perfo

230 transcription polymerase chain reaction (qRT-PCR) amplification of miRNA extracted directly from 500

231 lfite followed by polymerase chain reaction (PCR) amplification of specific regions of interest that,

232 was performed by polymerase chain reaction (PCR) amplification of tail genomic DNA and resequencing.

233 s accomplished by polymerase chain reaction (PCR) amplification of the 16S rRNA and hsp65 genes.

234 ncing followed by polymerase chain reaction (PCR) amplification of the cyclin A promoter (-267/37) sh

235 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (>83%) DNA target

236 However, PCR-amplification of GC-rich templates is often hampered

237 proach includes the design and validation of PCR amplification on bisulfite-treated DNA and pyroseque

238 PCR amplification over GC-rich and/or long repetitive se

239 icient ligation to target DNA and subsequent PCR amplification primed by the T oligo alone.

240 s an algorithm for reverse transcription and PCR amplification primer design using all of the publica

241 was generated by polymerase chain reaction (PCR) amplification, primer-walking sequencing and fragme

242 indered by the need of standard or real-time PCR amplification procedures.

243 detection without reverse transcription and PCR amplification processes.

244 ORF14, ORF16, and Ori regions of pMR during PCR amplification produced a temperature-sensitive plasm

245 PCR/ESI-MS) and base composition analysis of PCR amplification products can quickly and accurately id

246 pectrometry and base composition analysis of PCR amplification products from highly conserved genomic

247 plied a genomewide adapter-mediated emulsion PCR amplification protocol for ancient mammalian samples

248 ures as well as other microarray designs and PCR amplification protocols.

249 eet the criteria for success with one of two PCR amplification protocols.

250 lated positively with the higher sample-wide PCR amplification rate, lower template-to-template PCR b

251 In contrast to PCR amplification, RCA using phi29 DNA polymerase does n

252 in real-time, without any cDNA synthesis or PCR amplification requirements.

253 ive restriction endonucleases followed by AP-PCR amplification resulted in the detection of unknown u

254 The PCR amplification results of extracted DNA from the thre

255 udies of the intraepithelial lymphocytes and PCR amplification revealed surface expression of CD3 and

256 During polymerase chain reaction (PCR) amplification, selective nucleotides included at th

257 was purified, and polymerase chain reaction (PCR) amplification showed similar threshold cycle values

258 icroplate format without the need for sample PCR amplification, showing the potential suitability of

259 s may be caused in part by a bias during the PCR amplification step that discriminates against longer

260 ompleting the assay are included in a single PCR amplification step, assay costs, preparation time, a

261 abeling, and replicate clones created during PCR amplification steps can be identified and assigned t

262 rmance in terms of DNA purity and yield, and PCR amplification success as measured by using three dif

263 tes in a large, downloaded DNA database, and PCR amplification supported the general organization of

264 Most PCR amplification systems provided similar detection thr

265 PCR amplification targeting the 16S rRNA gene was used t

266 Although PCR amplification targeting this region is considered hi

267 Using PCR amplification techniques, a genomic clone correspond

268 After bulk PCR amplification, the droplets are lysed and the beads

269 above the microfluidic chip, which expedited PCR amplification to 18.8 min for a 30-cycle, three-temp

270 s verified by in vitro reverse transcriptase-PCR amplification to a single fragment of total cDNA obt

271 ease IV, followed by primer extension and/or PCR amplification to detect the endonuclease-generated s

272 kbone that is sufficiently biocompatible for PCR amplification to generate a cDNA library for RNAseq.

273 NA from low-abundance RNA and the subsequent PCR amplification under conditions which provide amplico

274 subjected to insert-specific primer-mediated PCR amplification using a high-fidelity DNA Pfu polymera

275 PCR amplification using COnsensus DEgenerate Hybrid Olig

276 P and 2-thioTTP were optimal to best support PCR amplification using thermostable polymerases of a si

277 When PCR amplification was combined with DNA extraction, 50%

278 PCR amplification was done for nine highly polymorphic s

279 Real-time PCR analysis showed that the PCR amplification was not impacted by time for any swab

280 PCR amplification was performed targeting 16S rRNA/tRNA(

281 A method using adapter-ligation and PCR amplification was successfully applied to visualize

282 al limit of detection (LOD) of the Genedrive PCR amplification was tested with genomic DNA; the perfo

283 TaqMan real-time PCR amplification was used to detect the presence of H.

284 and quantity for polymerase chain reaction (PCR) amplification was recovered from the MIL extraction

285 e modified, since cDNA yields obtained by RT-PCR amplification were severely reduced and no infectiou

286 irect DNA sequence analysis of exon 15 after PCR amplification were used.

287 PCR amplifications were performed for T-Ag sequences, an

288 transcription and polymerase chain reaction (PCR) amplification were no longer needed.

289 Previous methods have relied on PCR amplification, which is problematic due to primer de

290 argeted resequencing, including microfluidic PCR amplification, which may be enhanced by multiplex PC

291 HPV was detected by PCR amplification with PGMY09 and PGMY11 L1 primer pools

292 PCR amplification with primers not reaching a high speci

293 Bisulfite-modified DNA is subjected to PCR amplification with primers that would differentiate

294 tion, followed by reverse transcription, and PCR amplification with real-time fluorescence detection

295 system for picoliter droplet generation and PCR amplification with real-time fluorescence detection

296 Using PCR amplification with targeted and degenerate primers f

297 A gene amplicon sequencing based on two-step PCR amplifications with tagged primers for plates, rows,

298 verse transcription and 30 cycles of PCR (RT-PCR) amplification with capillary electrophoresis (CE) s

299 ing for reverse transcription and subsequent PCR amplification without droplet motion.

300 of substrate pairs, in vitro selection, and PCR amplification, yet does not require reaction conditi