ライフサイエンスコーパス: PCR assay

1 as measured by an ultrasensitive single-copy PCR assay.

2 A fraction was quantified by quantitative RT-PCR assay.

3 la invasion antigen, IpaH3, into a multiplex PCR assay.

4 NAs (miRNA), termed S-Poly(T) Plus real-time PCR assay.

5 edding and VL using a real-time quantitative PCR assay.

6 g a previously validated EV-D68-specific rRT-PCR assay.

7 and 65 (47%) were positive by the real-time PCR assay.

8 genes were assayed by quantitative real-time PCR assay.

9 positive visits were measured by a real-time PCR assay.

10 A-Seq were corroborated through real-time RT-PCR assay.

11 priate for targeting in a strain-specific RT-PCR assay.

12 viral, and 3 parasite) commercial multiplex PCR assay.

13 is was performed with a laboratory-developed PCR assay.

14 red by a differential real-time quantitative PCR assay.

15 copies/ml) was determined by a quantitative PCR assay.

16 r HSV 1/2 analyte-specific reagent real-time PCR assay.

17 T-PCR, and a serotype-specific multiplex rRT-PCR assay.

18 ies, each using a different quantitative CMV PCR assay.

19 , a dual-target total nucleic acid real-time PCR assay.

20 rcial stool antigen assays, and a commercial PCR assay.

21 ty and technical time may be possible with a PCR assay.

22 th 1484 (12%) of these cases confirmed on RT-PCR assay.

23 the reliance on more expensive and laborious PCR assays.

24 NA was isolated and used as template for the PCR assays.

25 e G and P genotyped using nested VP4 and VP7 PCR assays.

26 transcription factors was analyzed by using PCR assays.

27 ound to be more sensitive than the real-time PCR assays.

28 f acanthamoebae with four separate real-time PCR assays.

29 en, the Netherlands) and published multiplex PCR assays.

30 rformed for MSH2, along with allele-specific PCR assays.

31 dant with those determined by locus-specific PCR assays.

32 l suitable for highly sensitive quantitative PCR assays.

33 ith mRNA detection based principally upon RT-PCR assays.

34 ssion in single samples using repetitive qRT-PCR assays.

35 as revealed by chromatin immunoprecipitation-PCR assays.

36 in West Africa by polymerase-chain-reaction (PCR) assay.

37 itative real-time polymerase chain reaction (PCR) assay.

38 transcription-polymerase chain reaction (RT-PCR) assay.

39 microscopy and a polymerase-chain-reaction (PCR) assay.

40 triplex real-time polymerase chain reaction (PCR) assay.

41 croscopy and by a polymerase chain reaction (PCR) assay.

42 -transcriptase-polymerase-chain-reaction (RT-PCR) assay.

43 use in multiplex polymerase chain reaction (PCR) assays.

44 and mycobacterial polymerase chain reaction (PCR) assays.

45 donors whose samples tested positive on the PCR assay 2 to 7 months after the implicated donation.

46 by culture and negative for norovirus by RT-PCR assay; 4 specimens were positive for rotavirus by en

47 he present study, we evaluated two real-time PCR assays against novel targets in the mecA gene as an

48 ate that both ELISAs and the three real-time PCR assays allow the detection of traces of mustard in r

49 00 ng DNA per PCR tube, the duplex real-time PCR assay allowed the detection of white, black and brow

50 his contribution presents a single real-time PCR assay allowing the determination of the deer content

51 The paper presents a triplex real-time PCR assay allowing the simultaneous detection of three m

52 nsitive nested reverse transcription-PCR (RT-PCR) assay allowing the rapid detection of TiLV in fish

53 ea lion populations and results suggest that PCR assays alone may grossly underestimate ZcAV exposure

54 The real-time PCR assay also identified two samples with mixed P. falc

55 ted with both ELISAs and the three real-time PCR assays although mustard was not indicated on the foo

56 The new 23S-5S IGS nested PCR assay amplified all 11Rickettsiaspp., but the assays

57 The RT-PCR assay amplifies a segment of the VP1 gene, with an a

58 step real-time reverse transcription PCR (RT-PCR) assays; an accurate sample-to-answer RT-PCR test wo

59 ng using another commercially available HAdV PCR assay and a molecular typing assay for species ident

60 uenza infection was confirmed by means of RT-PCR assay and culture of nasopharyngeal swabs obtained f

61 as demonstrated by using WHV strain-specific PCR assays and (i) finding WHVNY relaxed circular DNA in

62 Using quantitative PCR assays and a fluorescence miRNA sensor, we show that

63 pective samples were resolved with alternate PCR assays and bidirectional sequencing of amplicons.

64 na in situ hybridisation (ISH), quantitative PCR assays and biomarkers of productive and transforming

65 re and broad-range and E. rostratum-specific PCR assays and confirmed the presence of fungal DNA in 2

66 StaphSR) and negative for MRSA by all three PCR assays and culture.

67 ovata blooms, and they were analyzed by both PCR assays and LC-HRMS.

68 We used degenerate PCR assays and massively parallel sequencing (MPS) to id

69 ime points was confirmed by variant-specific PCR assays and sequence analysis of single-colony cloned

70 , 41 (29%) were positive by the conventional PCR assay, and 65 (47%) were positive by the real-time P

71 for Orientia tsutsugamushi, three different PCR assays, and an IgM IFA.

72 es by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278/31

73 ole in CODD and confirming that the specific PCR assays are an effective differential diagnostic tool

74 ethodological recommendations and commercial PCR assays assist standardization.

75 The detection limit of the qRT-PCR assay at 95% probability was 0.28 FFU/ml as determin

76 yme-linked immunosorbent assay (ELISA) and a PCR assay based on amplification of the pan-Aspergillus

77 ental conditions and identified by molecular PCR assay based on the ITS-5.8S rDNA.

78 Finally, a novel multiplex PCR assay, based on random amplified polymorphic DNA (RA

79 A dual-probe real time PCR assay, based on the simultaneous detection of two Ta

80 ients tested negative for T. cruzi DNA on rt-PCR assay beyond day 14, except for 2 patients in the lo

81 y 40) by means of polymerase-chain-reaction (PCR) assay, but the last positive culture was identified

82 beta-d-glucan (BDG) test, and an Aspergillus PCR assay by using BAL fluid samples from immunocompromi

83 Our 384-well EGFL7 immuno-PCR assay can detect 0.51pM EGFL7 in plasma, approximate

84 ignal produced from probe-based RT-RPA or RT-PCR assays can be monitored using LEDs and a smartphone

85 C, Salt Lake City, UT), a highly multiplexed PCR assay, can identify 24 etiologic agents of sepsis (8

86 he 24 human chromosomes that allow for rapid PCR assays capable of capturing the genomic landscape of

87 We compared an FDA-cleared rapid (<20 min) PCR assay (Cobas Liat; Roche Diagnostics) to our routine

88 fic real-time reverse transcriptase PCR (rRT-PCR) assay described by F.

89 The beacon-based PCR assay designed in the present study is highly specif

90 The RT-PCR assay detected a virus in 59 samples (78%).

91 qRT-PCR assay detected higher miR-214 expression in the plas

92 Of the 200 specimens tested, real-time RT-PCR assay detected influenza A or B virus in 116 samples

93 Our PCR assays detected DNAs of various infectious agents in

94 The more sensitive PCR assay detects parasites earlier than microscopy, and

95 f matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be

96 In addition, the RT-RPA and RT-PCR assays do not cross-react with dengue and chikunguny

97 The PCR assay does not show cross-reactivity with 20 animal

98 The duplex real-time PCR assay does not show cross-reactivity with other Bras

99 sing real-time polymerase-chain-reaction (rt-PCR) assays, during the treatment period and 10 months a

100 oped test (LDT) and 4 commercially available PCR assays: EraGen Multicode (Luminex, Austin, TX), Focu

101 Surprisingly, a PCR assay failed to detect SFV infection in any of these

102 a fully automated respiratory viral panel RT-PCR assay (FilmArray).

103 isolates was used in both PCR and real-time PCR assays followed by electrophoresis or melting temper

104 uantitative multiplexed methylation-specific PCR assay for a panel of ten genes, consisting of novel

105 erium abscessus group, a multiplex real-time PCR assay for clarithromycin resistance showed 95% (38/4

106 ly developed a TaqMan-based real-time duplex PCR assay for detection of the A2058G mutation, and upon

107 report the development of a robust real-time PCR assay for determining GBS serotypes.

108 etermine the clinical utility of a real-time PCR assay for diagnosis of congenital CMV infection.

109 vailable genomic sequence data to evaluate a PCR assay for distinguishing Campylobacter species.

110 nducible Cas9 (ciCas9) and a droplet digital PCR assay for double-strand breaks (DSB-ddPCR) to invest

111 Through use of a quantitative PCR assay for HHpgV-1, infection was also detected in 17

112 this study, we compared a routine real-time PCR assay for HSV types 1 (HSV-1) and 2 (HSV-2) to a rec

113 ubjects that assessed the performance of any PCR assay for invasive aspergillosis in whole blood or s

114 ice junction, we developed a quantitative RT-PCR assay for K-Ras4A and K-Ras4B message capable of mea

115 ssay was more appropriate than the real-time PCR assay for monitoring the response to treatment.

116 re, we describe a SYBR green-based real-time PCR assay for Plasmodium species identification from who

117 We describe a highly specific PCR assay for the authentic identification of pork.

118 Lyra Direct HSV 1+2/VZV multiplex real-time PCR assay for the detection and differentiation of herpe

119 rformance of a simple, inexpensive real-time PCR assay for the detection of 13 carcinogenic HPV types

120 lopment and validation of a TaqMan real-time PCR assay for the detection of E. rostratum in body flui

121 difficile universal direct PCR, a real-time PCR assay for the detection of the C. difficile toxin B

122 difficile universal direct PCR, a real-time PCR assay for the detection of the C. difficile toxin B

123 ment and evaluation of a multiplex real-time PCR assay for the detection of these 3 Rickettsia specie

124 the present study was to develop a real-time PCR assay for the identification and quantification of f

125 The paper presents a TaqMan real-time PCR assay for the quantitative determination of roe deer

126 The paper presents a duplex real-time PCR assay for the simultaneous detection of three potent

127 Real-time PCR assays for beta-globin and Universal 16S rRNA gene t

128 gleplex real-time reverse transcriptase (RT) PCR assays for broad detection of all four mHEV genotype

129 We developed novel quantitative PCR assays for cell-associated (CA) HIV-1 DNA and RNA, t

130 (assays A and B) and two duplex real-time RT-PCR assays for detection and differentiation of mHEV gen

131 The high sensitivity of PCR assays for diagnosing Clostridium difficile infectio

132 valence, we developed real-time quantitative PCR assays for each virus and screened additional DNA sa

133 dized approaches (Fusarium disease severity, PCR assays for Fusarium spp. identification and mycotoxi

134 We transitioned laboratory-developed PCR assays for herpes simplex virus 1 (HSV-1), HSV-2, an

135 e of the RP panel in comparison to real-time PCR assays for the detection of respiratory pathogens.

136 subjected to bacterial isolation studies and PCR assays for three phylogroups of relevant DD treponem

137 a HAdV-specific laboratory-developed PCR (LD-PCR) assay for confirmation.

138 multiplex real-time quantitative RT-PCR (qRT-PCR) assay for DENV-1, -3, and -4 detection but 10-fold

139 quantitative reverse transcription-PCR (qRT-PCR) assay for detection of OPV Sabin strains 1, 2, and

140 ed a real-time reverse transcriptase PCR (RT-PCR) assay for the detection of human enterovirus D68 (E

141 ltiplex real-time polymerase-chain reaction (PCR) assays for 26 respiratory bacteria and viruses.

142 oped quantitative polymerase chain reaction (PCR) assays for absolute quantification of mRNA and pre-

143 ies and real-time polymerase-chain-reaction (PCR) assays for B. microti DNA on blood-donation samples

144 RSV) real-time reverse transcription-PCR (RT-PCR) assays for detection of genetically diverse PRRSV i

145 -transcriptase-polymerase-chain-reaction (RT-PCR) assays for ZIKV in blood, cerebrospinal fluid, and

146 ormed using a laboratory-developed real-time PCR assay; for the postimplementation group, CSF samples

147 tative real-time reverse transcriptase (qRT) PCR assays from the Centers for Disease Control and Prev

148 iae, and existing polymerase chain reaction (PCR) assays generally target large gene segments that ma

149 aph ID/R blood culture panel (BCP) multiplex PCR assay (Great Basin Scientific, Salt Lake City, UT) f

150 The nested PCR assay had an overall sensitivity of 42.5%, a specifi

151 Polymerase chain reaction (PCR) assays have greatly improved the sensitivity detect

152 of the BD Max enteric bacterial panel (EBP) PCR assay in comparison to culture for the detection of

153 e for an in-house reverse transcription (RT)-PCR assay in testing of influenza-positive clinical samp

154 (kappa of >/=0.723) with the RT-LAMP and qRT-PCR assays in detecting the dengue viremic samples.

155 demonstrates greater sensitivity than nested PCR assays in FFPE tissues and provides an effective met

156 lico approaches for designing and validating PCR assays in the clinical microbiology laboratory.

157 the gold-standard polymerase chain reaction (PCR) assay in an operator-blinded study.

158 transcription-polymerase chain reaction (qRT-PCR) assay in support of pharmacokinetic and toxicokinet

159 -transcriptase-polymerase-chain-reaction (RT-PCR) assay in urine or blood in an enhanced arboviral cl

160 Here we describe a triplex qRT-PCR assay, including assembly and evaluation for sensiti

161 qRT-PCR assay indicated that compound 1 and DBL exposure up-

162 Real-time PCR assays indicated that Etv4 and Etv5 mRNAs are signif

163 results also demonstrate that the real-time PCR assay is extremely susceptible to contamination and

164 tients even when an IDMS-traceable enzymatic Pcr assay is used.

165 ssibility for some assays, in well-optimized PCR assays it can reduce the number of amplifiable targe

166 Current RT-PCR assays lack sensitive and reliable positive controls

167 ffectiveness, and the simplicity of the ARMS-PCR assay make it a suitable tool to monitor HIVDR patte

168 Commercial PCR assays may have a standardized methodology while pro

169 ssay results it has been concluded that SDRT-PCR assay might be an efficient tool for the verificatio

170 recombination was evaluated by quantitative PCR assay of genomic DNA from liver and spleen.

171 A commercial PCR assay of perirectal swab specimens detected 17 (68%)

172 The performance of a PCR assay of serum was not significantly different from

173 proficiency testing surveys for quantitative PCR assays of cytomegalovirus (CMV), Epstein-Barr virus

174 -transcriptase-polymerase-chain-reaction (RT-PCR) assays of respiratory samples.

175 The TaqMan-based real-time triplex PCR assay offers an alternative to conventional nested P

176 fficiency of the SAF microlens array, the SP-PCR assay on the LOC platform demonstrated a high sensit

177 y assessed the impact of a direct HSV (dHSV) PCR assay on the time to result reporting and the durati

178 report results of measurements from multiple PCR assays, on four human genomic DNAs treated with four

179 In contrast to conventional quantitative PCR assays or decoy cell counts, quantitative urinary PV

180 eceiving benznidazole, tested positive on rt-PCR assay (P<0.001 for the comparison of the benznidazol

181 zole, tested positive for T. cruzi DNA on rt-PCR assay (P<0.01 for the comparison of the benznidazole

182 stration authorized emergency use of the rRT-PCR assay panel as an in vitro diagnostic test for MERS-

183 We evaluated a genus- and group-specific PCR assay panel using 284 prosthetic knee synovial fluid

184 The Abbott, EraGen, Elitech, Focus, and LDT PCR assays performed similarly (kappa >/= 0.89) for the

185 improved buffers and enzymes for seminested PCR assays provided higher specificity and sensitivity f

186 action of RNA, the multiplex quantitative RT-PCR assay provides rapid diagnosis for the differential

187 wed an agreement of 97.4% with the real-time PCR assay regarding 464 pathogens found in the clinical

188 This new phylogenomics-based PCR assay represents a valuable tool for rapid typing of

189 -house nested PCR and quantitative real-time PCR assays, respectively.

190 u(+) real-time reverse transcription-PCR (RT-PCR) assay results.

191 Quantitative PCR assays revealed that HNF4A and PTBP1 mRNAs were up-

192 sing 10 different real-time quantitative CMV PCR assays run by eight different laboratories.

193 The multiplex RT-PCR assay showed 95% sensitivity and 100% specificity fo

194 , chromatin immunoprecipitation-quantitative PCR assay showed enrichment of p63 on Sun1, Syne3, and P

195 ) to our routine influenza A and B real-time PCR assay (Simplexa Flu A/B & RSV Direct; Focus Diagnost

196 2 (HSV-2) to a recently FDA-approved direct PCR assay (Simplexa HSV-1/2 Direct; Focus Diagnostics, C

197 tion of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with new

198 otal) and three in-house developed real-time PCR assays (singleplex assay for white mustard, singlepl

199 Using a quantitative reverse transcription PCR assay specific for all HIV-1 mRNAs, we demonstrate t

200 The method consisted of a real-time PCR assay targeting the gene encoding for the germ agglu

201 The method consists on a real-time PCR assay targeting the ITS1 region, using a nuclease (T

202 A highly sensitive TaqMan real-time PCR assay targeting the mitochondrial 12S rRNA gene was

203 from GFE, we used 84 for the development of PCR assays targeting putative canine-associated genetic

204 ing a mouse anti-Zika virus antibody, and RT-PCR assays targeting the NS5 and envelope genes.

205 ted a laboratory-developed real-time PCR (LD-PCR) assay targeting stx1, stx2, and rfbEO157 with 2,386

206 eport a tetraplex polymerase chain reaction (PCR) assay targeting two different DNA regions in beef (

207 sing quantitative polymerase chain reaction (PCR) assays targeting the 16S ribosomal RNA gene.

208 two real-time reverse transcription-PCR (rRT-PCR) assays targeting the MERS-CoV nucleocapsid (N) gene

209 n using real-time polymerase chain reaction (PCR) assays targeting the opa gene and porA pseudogene.

210 with real-time reverse transcription-PCR (RT-PCR) assays targeting the ZKV envelope and NS2B genes.

211 we developed an HML-2-specific quantitative-PCR assay that detects 51 of the 89 known HML-2 provirus

212 sequence, we developed a multiplex touchdown PCR assay that in a single reaction can confirm the spec

213 We demonstrate a simple droplet digital PCR assay that quickly quantitates a range of indel muta

214 equence typing (MLST) system and a screening PCR assay that targets ST131-specific sequence polymorph

215 concentrations of </= 1.8 M, compared with a PCR assay that was functional at concentrations of <100

216 data to evaluate a duplex real-time PCR (RT-PCR) assay that targets mapA and ceuE for the detection

217 volving real-time polymerase chain reaction (PCR) assays that facilitate direct analysis of a single

218 llele-specific polymerase chain reaction (AS-PCR) assays that we developed for Bruton tyrosine kinase

219 were positive by an alternative real-time RT-PCR assay (the Trombley assay); three (17%) of 18 sample

220 ere then tested using four different DENV RT-PCR assays: the pan-DENV assay, a commercially produced,

221 Compared to fungal culture and the previous PCR assays, this real-time PCR assay was more sensitive.

222 With the use of a long quantitative PCR assay, tiron (EC50 10 mM) was found to confer comple

223 nsitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp.

224 ay to detect antibodies to NWM SFV, a nested PCR assay to detect NWM SFV DNA, and a beta-galactosidas

225 emonstrated the suitability of the real-time PCR assay to detect the presence of low percentages of h

226 The CDC developed a multitarget PCR assay to differentiate B. pertussis, B. holmesii, an

227 omic data, we developed a one-step multiplex PCR assay to identify Salmonella and simultaneously diff

228 meric proteins, we used an emulsion-based RT-PCR assay to link and amplify TCR pairs.

229 Using a SYBR Green quantitative PCR assay to observe Common Carp ( Cyprinus carpio ) eDN

230 We evaluated a real-time PCR assay to predict ciprofloxacin susceptibility using

231 ing the concentration obtained with the deer PCR assay to that obtained with a reference system which

232 Additionally, we compared the qRT-PCR assay to the gold standard direct fluorescent-antibo

233 The ability of PfLDH- or PfIDEh-based immuno-PCR assays to detect <1 parasite/microL suggests that im

234 ed 783 subjects with RDT-/PCR+ results using PCR assays to detect and confirm deletions of the pfhrp2

235 y-to-perform and interpret PCR and real-time PCR assays to identify C. auris and related species: Can

236 f South Africa were used in duplex real-time PCR assays to simultaneously detect A. marginale and A.

237 loped a sensitive polymerase chain reaction (PCR) assay to quantify the HBV RNA load in a supernatant

238 cing and novel reverse transcription-PCR (RT-PCR) assays to distinguish all RNA species in the KSHV l

239 irus (EBV) load testing in four quantitative PCR assays, treating digital PCR as a reference assay.

240 for BVDV--two reverse transcriptase PCR (RT-PCR) assays, two commercial real-time RT quantitative PC

241 rrelated well with those of the real-time RT-PCR assays used as benchmarks.

242 A real-time, multiplex PCR assay using HRM was designed for the detection of pl

243 Quantitative RT-PCR assays using apical tissues showed that GA biosynthe

244 herefore, integrated DNA barcodes, Real-Time PCR assays using TaqMan probes and UHPLC-HR-MS system pr

245 species-specific polymerase chain reaction (PCR) assay using conserved regions of mitochondrial DNA

246 , sensitive, specific molecular-beacon-based PCR assay, using primers against pfoB gene of Trichomona

247 with those of laboratory-developed real-time PCR assays, using a variety of previously collected clin

248 The emergence of real-time PCR assays utilizing allele-specific molecular detection

249 timization, the agreement with the reference PCR assay was 100% (123/123) for HSV-1, 96.7% (119/123)

250 ether, all data indicated that this hexaplex PCR assay was a simple, fast, sensitive, specific, and c

251 The ARMS-PCR assay was able to detect M184V, T215Y/F, K103N, and

252 The real-time PCR assay was able to detect the presence of bla(KPC) in

253 In addition, the real-time PCR assay was applied to the analysis of commercially av

254 The duplex real-time PCR assay was applied to verify correct labelling of com

255 Suitability of PCR assay was confirmed in raw (n = 20), cooked (60, 80

256 c species ofRickettsia The nested 23S-5S IGS PCR assay was coupled with reverse line blot hybridizati

257 A PCR assay was designed to amplify size-distinguishable f

258 lification refractory mutation system (ARMS)-PCR assay was developed and used to investigate the most

259 A real-time PCR assay was developed for detection of crab, a crustac

260 A hexaplex-conventional PCR assay was developed for identification of five meat

261 A novel nested PCR assay was developed to detectRickettsiaspp. in ticks

262 The performance and reproducibility of PCR assay was evaluated by composite reference standard

263 The multiplex PCR assay was found to be a specific and sensitive metho

264 and the previous PCR assays, this real-time PCR assay was more sensitive.

265 A duplex PCR assay was set up requiring only 1000 sorted cells fo

266 The multiplex PCR assay was specific at discriminating each species fr

267 A PCR assay was used on brain and other available tissues

268 The Quantifiler Kit TaqMan quantitative PCR assay was used to measure the recovery of DNA from h

269 Furthermore, a multiplex PCR assay was validated for rapid molecular differentiat

270 The novel PCR assay was validated using clinical samples from huma

271 gnostic performance of whole-blood and serum PCR assays was moderate, with a sensitivity and specific

272 The PCR assays was performed to detection of H. pylori, vacA

273 ea, positivity of N. gonorrhoeae DNA on both PCR assays was present at days 7 or 14 in 13% (95% confi

274 A panel of RT-PCR assays was used to detect noninfluenza respiratory v

275 At baseline, a polymerase-chain-reaction (PCR) assay was performed on blood samples obtained from

276 and sequential chromatin immunoprecipitation-PCR assay, we found that ZNF322A could form a complex wi

277 The LOD and LOQ of the real-time PCR assay were 0.1% and 0.4%, respectively.

278 icile by the GeneXpert C. difficile/Epi tcdB PCR assay were tested with the rapid membrane C.

279 When the ELISAs and real-time PCR assays were applied to the analysis of 15 commercial

280 PCR assays were developed for each clade-specific marker

281 New multiplex PCR assays were developed for mating-type determination

282 Three quantitative PCR assays were developed to target gamma-proteobacteria

283 R group" (the results of serial serum GM and PCR assays were provided to treating physicians) and "GM

284 AS-PCR assays were used to screen patients with and without

285 the relative diagnostic sensitivity of CDC's PCR assay when using a different PCR master mix.

286 We have designed a novel triplex real-time PCR assay which simultaneously amplifies the MT-ND4 gene

287 nformation of ITS2, we developed a Real-Time PCR assay which successfully identified herbal material

288 was achieved over the existing real-time RT-PCR assays, which require denaturation of the RVA double

289 The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.

290 PCR assay with blood specimens from high-risk hematology

291 lidated the first direct multiplex real-time PCR assay with melt curve analysis for the identificatio

292 e also compared the utility of the real-time PCR assay with that of the previously described beta-d-g

293 A multitarget real-time PCR assay with three targets, including insertion sequen

294 The limit of detection for FilmArray and qRT-PCR assays with inactivated ZEBOV, based on duplicate po

295 A quantitative real-time PCR (qRT-PCR) assay with single-copy sensitivity targeting HIV-1

296 -transcriptase-polymerase-chain-reaction (RT-PCR) assay with the use of the target sequences of NP an

297 -transcriptase-polymerase-chain-reaction (RT-PCR) assays with independent RNA extraction from throat

298 en tested by different real-time and digital PCR assays, with various magnitudes of bias compared to

299 -transcriptase-polymerase-chain-reaction (RT-PCR) assay, with consistent findings on electron microsc

300 To help ensure that PCR assays yield consistent results over time and place,