ライフサイエンスコーパス: PCR product

1 haI-assay and sequencing of cloned bisulfite PCR products).

2 of each clone for the presence or absence of PCR product.

3 or with the ss extension of the next-in-line PCR product.

4 above 95% in the central 56% of the average PCR product.

5 criptase (RT)-PCR and sequencing of the bulk PCR product.

6 d primers that amplify a Balamuthia-specific PCR product.

7 n unambiguous base composition for a 120-mer PCR product.

8 nblocked prior to hybridization with labeled PCR product.

9 , which were used in PCR to amplify a 137-bp PCR product.

10 done efficiently without purification of the PCR product.

11 ons when the 4-bromopyridone is present in a PCR product.

12 er from a linearized plasmid or from a crude PCR product.

13 strain typing, and Sanger sequencing of bulk PCR product.

14 igh-resolution melt analysis to characterize PCR products.

15 ous and seamless assembly of three different PCR products.

16 (SNP-PCR), and (3) optical detection of the PCR products.

17 electrospray ionization mass spectrometry of PCR products.

18 ifferentiation between predicted alternative PCR products.

19 ) patients with FPAH had abnormally sized RT-PCR products.

20 display method with quantitative analysis of PCR products.

21 pecies clones per patient from serum-derived PCR products.

22 PCR) and by analysis of the sequences of the PCR products.

23 gle-base mismatch cleavage of heteroduplexed PCR products.

24 he TSPY gene in both purified and unpurified PCR products.

25 PCR (RT-PCR) and restriction digestion of RT-PCR products.

26 eed for purification and denaturation of the PCR products.

27 opy, culture, and gel electrophoresis of the PCR products.

28 melting curves and denaturation profiles of PCR products.

29 ed and, thus, enhanced the brightness of the PCR products.

30 for TA cloning of polymerase chain reaction (PCR) products.

31 ect sequencing of polymerase chain reaction (PCR) products.

32 8 and 126 bp fragments for CT and undigested PCR product 294 bp indicating CC genotype.

33 The PCR products (294 bp) was subsequently digested with Hin

34 Real-time PCR is widely employed to quantify PCR products across a range of applications.

35 e variation (electrophoretic karyotypes) and PCR products (AFLP procedures) were determined for Micro

36 inoculated animals had a P. carinii-specific PCR product after infection.

37 particular short polymerase chain reaction (PCR) product allowed this nonbinding sequence to overtak

38 the free primer and not the double-stranded PCR product, allowing real-time fluorescent quantificati

39 typing strategies based on binary readout of PCR product amplification and size selection.

40 nal bisulfite capillary sequencing of cloned PCR products amplified from a subset of the selected reg

41 We validated the approach on two long PCR products amplified from the human genome and confirm

42 n different days, using different batches of PCR product and different sphere segment void (SSV) subs

43 aries of >10(7) transformants from a pool of PCR products and a linearized plasmid vector.

44 The 30 primers were combined with the PCR products and biotinylated dideoxynucleotides for SBE

45 the intrinsic charge of multiple unprocessed PCR products and does not require sample processing or a

46 Sequencing intron 9 RT-PCR products and genomic DNA in each case revealed homoz

47 Sequencing of 16S PCR products and Gram staining showed that the majority

48 tocol utilizes only submicroliter volumes of PCR products and is compatible with multiple capillary D

49 d oligochromatographic dipstick detection of PCR products and is highly sensitive for use in humans b

50 derived from large insert clones as well as PCR products and oligonucleotides.

51 pancreatic tissue by sequence analysis of RT-PCR products and quantification using real-time PCR.

52 pancreatic tissue by sequence analysis of RT-PCR products and quantification using real-time PCR.

53 as confirmed by E1/\E4 reverse transcription-PCR products and RhPV-d sequence identity with the donor

54 approach based on different lengths of Aly8 PCR products and show that most A. lyrata haplotypes hav

55 was done by restriction enzyme digestion of PCR products and TaqMan SNP genotyping.

56 Gene probes were constructed from the PCR products and used to classify isolates based on hybr

57 , the replacement of human DNA template with PCR product, and the coating of the microchannel surface

58 trode surface as synthetic oligonucleotides, PCR products, and RNA transcripts are all detected with

59 m DNA was confirmed by sequencing the cloned PCR products, and while alignment of the ITS amplicons s

60 the adapter and the T-DNA; sequencing of the PCR products; and identification of the flanking sequenc

61 The fluorescein-labeled PCR products are amplified and electrophoretically analy

62 quence, helping ensure that only the desired PCR products are analyzed.

63 re 50-100 SNPs/capillary/run if the sizes of PCR products are arranged at every 5-10 bases from 100 t

64 To recover a fragment of interest, PCR products are cloned and sequenced.

65 The PCR products are converted back into circular plasmids b

66 The resulting PCR products are electrophoretically injected onto the C

67 PCR products are prepared in wholly double-stranded homo

68 Biotin-labeled PCR products are spotted, unpurified, onto a streptavidi

69 abundant transcript generating a 1.2-kbp RT-PCR product) arising from an unspliced precursor likely

70 nrichment strategy for DNA capture that uses PCR products as subgenomic traps.

71 erating a 1.2-kbp reverse transcription [RT]-PCR product) as well as viral assembly on the cell membr

72 cle sequencing of polymerase chain reaction (PCR) products at kilobase scale, without conventional DN

73 tion discovery from the sequences of diploid PCR products, automated and reliable detection of indels

74 identified genes is then designed, with each PCR product being of a different size to allow multiplex

75 alytic activity of the MPNP, the analysis of PCR product bound MPNP yielded a sensitivity of 10(3) CF

76 ne synovial fluid) yielded an atypical oppA1 PCR product, but no atypical results were detected befor

77 ion abundance of the oligonucleotide and the PCR product by more than 6-fold relative to the best ach

78 The detection of PCR products by a surface-enhanced Raman scattering (SER

79 ltaneous mutation scanning and genotyping of PCR products by high-resolution DNA melting analysis.

80 sRNA was synthesized from PCR products by in vitro transcription (IVT).

81 -labelled DNA was conveniently prepared from PCR products by lambda-exonuclease digestion and strepta

82 primers, followed by characterization of the PCR products by microchip hybridization with oligonucleo

83 immediately accessible protocol to quantify PCR products, by measuring ethidium bromide fluorescence

84 h polymorphisms detected among ITS1 and ITS2 PCR products can differentiate 20 of 33 species of molds

85 set, chronic infections, and the quality of PCR products can lower the MS score but never affected t

86 lution melting of polymerase chain reaction (PCR) products can detect heterozygous mutations and most

87 The 900 PCR products cloned represented 833 unique sequences bel

88 alidate by RT-PCR and four of five sequenced PCR products confirm connectivity unambiguously.

89 Exome resequencing and sequencing of PCR products confirmed FN-induced deletions detected by

90 Cloning of PCR products confirmed that this was a 2-bp deletion mut

91 Southern blot analysis of SX-PCR products confirmed their mtDNA origin.

92 transcription polymerase chain reaction (RT-PCR) products confirmed the expression of five of these

93 or non-complementary 20-mer ssDNA or various PCR products consisting of 180-190 bp (dsDNA) were recor

94 The resulting bar-coded PCR products contain the adapters needed for Illumina se

95 ymorphism (SSCP) analysis was performed, and PCR products containing different patterns were cloned a

96 letion virus to form plaques was restored by PCR products containing single alanine substitutions wit

97 The resulting PCR products could be easily detected in gels based on t

98 DNA fragments, indicating that the sizes of PCR products could be validated with an integrated chip-

99 two multiplex reverse transcription-PCR (RT-PCR) products coupled with microarray-based systems for

100 With PCR products, current changes are specific to the bacter

101 Sequence analysis of two of these unique XL PCR products demonstrated two new LOS classes.

102 ged gene is determined by sequencing inverse-PCR products derived from genomic DNA.

103 sizing fragments from restriction digests of PCR products derived from two sets of 16S rDNA-specific

104 ll unmethylated cytosine bases with thymine, PCR products derived from unmethylated templates contain

105 en mixed together, the linearized vector and PCR products directionally assemble into a recombinant m

106 uses truncated recombination sites to clone PCR products directly into destination/expression vector

107 ed onto the MPNP surface which would bind to PCR product during PCR cycling.

108 was possible to detect hybridisation of mecA PCR product electrochemically at concentrations as low a

109 formation polymorphism (SSCP) analysis of RT-PCR products, elucidated the developmental and tissue-sp

110 topoisomerase-based method was used to clone PCR products encoding 991 of the 1041 open reading frame

111 y measuring ethidium bromide fluorescence of PCR products excised from agarose gels.

112 on breakpoint generates orientation-specific PCR products, exemplified by a genotyping assay for the

113 he PCR products with the USER enzyme leaving PCR products flanked by 3' ss extensions.

114 thod involving the purification of multiplex PCR products followed by uniplex analysis on a microflui

115 determined using direct manual sequencing of PCR products, followed by resequencing separately amplif

116 a genus-specific restriction analysis of the PCR product for conclusive identification of the unknown

117 RT-PCR products for AMPK-alpha1 and -alpha2 isoforms and li

118 RT-PCR products for Apos C-I, C-II, E, and J were found in

119 The preparation of LATE-PCR products for Dilute-'N'-Go sequencing takes only 30

120 equencing of bulk polymerase chain reaction (PCR) products (for 22 couples), clonal analysis (for 3),

121 The reduction in PCR product formation along the gradient of increasing D

122 ing cDNA clones and quantifying 6FAM-labeled PCR products found that although the healthy mother pred

123 quences were derived by direct sequencing of PCR products from 21 plasma specimens diluted to end poi

124 ectrometry (PCR/ESI-MS), designed to amplify PCR products from any strain of influenza virus and to g

125 estriction analysis and by sequencing cloned PCR products from bisulfite-converted DNA.

126 Following on these findings, we use PCR products from different cell types to identify novel

127 Sequencing reverse transcription-PCR products from five DEFL clusters confirmed our gene

128 Sequencing of RT-PCR products from five of the six alleles reveals a nonc

129 GC-clamp-containing PCR products from interrogated and wild-type samples are

130 Sequencing of PCR products from MAP cultures confirmed the presence of

131 in vivo were verified by the presence of the PCR products from the promoter and enhancer elements in

132 al, where PCR duplicates arise from multiple PCR products from the same template molecule binding on

133 ative nucleotide sequence analysis of cloned PCR products from three affected cats further suggested

134 res ranging from 1/99 to 50/50 of two 285-bp PCR products from two cell lines that differ at one nucl

135 Both purified and unpurified PCR products gave comparable results.

136 Pyrosequencing of domain amplicon and RCA PCR products generated 1.5 x 10(6) reads, including more

137 olate, based upon the presence or absence of PCR products generated from each target gene.

138 The digestion of the PCR products generates a 5'-overhang structure at the ta

139 acked 3' homology to the cut vector, or both PCR products had both 5' and 3' homology to the cut vect

140 The PCR product has a sequence identical with that of human

141 luorescence of dyes bound to double-stranded PCR products has been utilized extensively in various re

142 y of a 16.2 kDa oligonucleotide and a 53-mer PCR product in the high-pressure region between an elect

143 This allows us to load many PCR products in a single capillary/lane.

144 was used to directly sequence >100 bisulfite PCR products in a single sequencing run without subcloni

145 -mutagenesis only involves transformation of PCR products in bacteria expressing Red/ET recombineerin

146 ctions with Taq polymerase failed to produce PCR products in the presence of only small amounts of dt

147 r the GNAQ and GP1BB genes, different length PCR products in the range of 700 bases to 2 kilobases co

148 Subcloning and sequencing of these PCR products indicated that they reflected aberrant spli

149 mbination in yeast for efficient assembly of PCR products into programmed or random combinations of m

150 The HMC-1 renin RT-PCR product is 100% homologous to Homo sapiens renin.

151 the same order of magnitude as when a single PCR product is inserted alone into the display vector by

152 The asymmetric PCR product is purified and non-target transcripts conta

153 Furthermore, the same PCR product is suitable for additional analyses such as

154 The resulting PCR product is then cloned into pSub2 to generate a dive

155 Each PCR product is then specifically circularized by ligatio

156 The diluted initial PCR product is used as a template to check individual mi

157 in molecular biology where quantification of PCR products is necessary.

158 Conventional Sanger sequencing of PCR products is the gold standard for species authentica

159 ied or unpurified polymerase chain reaction (PCR) products labeled with a fluorophore and a biotin ar

160 mology to the cut vector and the second scFv PCR product lacked 3' homology to the cut vector, or bot

161 regardless of whether or not one of the scFv PCR products lacked 5' homology to the cut vector and th

162 strated for synthetic targets (LOD = 10 nM), PCR products (LOD = 100 pM), and direct, amplification-f

163 positive-selection vector ideal for cloning PCR products, making DNA libraries, as well as routine c

164 ies for checking, sizing and separating both PCR products, nucleic acids derived from in vivo or in v

165 ified by sequencing of reverse transcriptase-PCR products obtained from oral mucosal tissues, disclos

166 Transgenic plants containing a LR-PCR product of one of these four genes displayed broad s

167 A PCR product of the correct size was obtained from normal

168 PCR product of the five fragments was gel-purified and c

169 lymer microspheres to capture a biotinylated PCR product of the oligonucleotide sequence, which was s

170 The assay generated PCR products of 100, 119, 133, 155, 253, and 311 bp for

171 The amplified expected PCR products of 212 and 271 bp were observed for porcine

172 With the use of the PNAA-MEKC method, PCR products of 88, 134, 216, and 447 bases are clearly

173 tibody repertoires by sequencing the 5'-RACE PCR products of B-cell transcripts from IAVI donor 17 an

174 PCR products of four typing fragments were sequenced, in

175 Sequencing of PCR products of H. heilmannii and H. felis was done.

176 he capability for the discrimination between PCR products of normal, heterozygous, and homozygous bet

177 of CD4, CXCR4, and CCR5, or in the amount of PCR products of reverse transcription, DNA synthesis, an

178 The PCR products of Ruminant's samples with positive H. pylo

179 Unexpectedly, however, distinct PCR products of smaller molecular weight were noted in t

180 PCR products of the expected size were detected in all s

181 of the AGA926-928TTC H. pylori genotype and PCR products of two patients showed absence of the conse

182 present a method to identify single-stranded PCR products of varying lengths by hybridization of n-al

183 Cot, that measures the sequence diversity of PCR products on the basis of DNA hybridization kinetics,

184 a spotter for the deposition of the purified PCR products on the substrates and the purification step

185 loned DNA inserts to detailed analysis using PCR products or oligonucleotides of 100 bp or less.

186 ctly on the Escherichia coli chromosome with PCR products or synthetic single-stranded DNA (ssDNA) ol

187 relevant range and specifically detects the PCR products over reagents such as Taq polymerase and nu

188 cilitate the detailed analysis of bisulphite PCR products (particularly using multiple cloned templat

189 or protein-analysis, like DNA sequencing of PCR products, PCR-SSCP (polymerase chain reaction - sing

190 fully automated method to purify and desalt PCR products prior to analysis by electrospray ionizatio

191 ect sequencing of polymerase chain reaction (PCR) products, pyrosequencing, and cloning of PCR produc

192 Real-time monitoring of fluorescent PCR product (qPCR) was used to measure the fraction of D

193 ically feasible, and alternative methods for PCR product quantification can be expensive and time con

194 ust and simple method for direct, label-free PCR product quantification using an integrated microelec

195 imultaneously with detection of the Bcc recA PCR product; recA sequence cluster analysis also was par

196 previously published protocols: (i) smaller PCR products reduce primer numbers and amplification ste

197 , as well as with polymerase chain reaction (PCR) products related to different lineages of Mycobacte

198 Arrays for CGH based on PCR products representing assemblies of BAC or cDNA clon

199 althy donors using a microarray spotted with PCR products representing every gene from the seven S. a

200 acted from rats, we were able to demonstrate PCR products representing MOR1, MOR1(A), and MOR1(B) spl

201 fM (S/N=3.0) for 20-mer ssDNA and for dsDNA (PCR product), respectively.

202 ata are consistent with a model in which one PCR product results from the action of Tn5386 integrase,

203 kuADelta) strains of A. nidulans with fusion PCR products results in high frequencies of accurate gen

204 Sequence analysis of a 492-bp PCR product revealed comparable levels (0.2-4.5%) of int

205 nd restriction enzyme digestion of this wbiI PCR product revealed sufficient DNA polymorphisms to dis

206 DNA sequencing of the PCR products revealed a base alteration from C to T in F

207 dditionally, sequence analysis of the 5'RACE-PCR products revealed multiple transcriptional start sit

208 Sequencing of PCR products revealed points of recombination that lacke

209 Analysis of individual LAM-PCR products revealed that the AAV genomes were arranged

210 samples and results were comparable with the PCR product samples.

211 eotide polymorphisms which were confirmed by PCR product Sanger sequencing.

212 The PCR products, separated by PAGE and viewed after silver

213 f a portion of the DAPK promoter followed by PCR product sequencing confirmed dense hypermethylation

214 ungarotoxin binding and quantitative PCR and PCR product sequencing, we showed that the rat mast/baso

215 age charge states of the oligonucleotide and PCR product shifted from 12.5- to 14.5- and 10.9- to 12.

216 triction fragment length polymorphism of the PCR products showed that each of the four erythromycin-r

217 Sequencing of the PCR products showed that the alpha and beta chains are t

218 several microorganism taxa and sequenced all PCR products simultaneously by high-throughput sequencin

219 Both genes demonstrated PCR product size and nucleotide sequence variation.

220 y a pair of primer sequences and an expected PCR product size.

221 Detection of DNA restriction fragments and PCR product sizing is demonstrated using the electroacti

222 lian families by phylogenetic analyses using PCR products spanning gene boundaries.

223 Subsequent sequencing of PCR products spanning the breakpoints identified the bre

224 Sequencing of approximately 50,000 RT-PCR products spanning the exon 7-8 junction in 10 tissue

225 chain reaction (SP-PCR) to form the array of PCR products starting from the oligonucleotide primers.

226 Denaturing HPLC analysis of PCR products suggests that PtaAGP6 is encoded by a singl

227 PCR products suitable for sequencing were obtained for 1

228 a rapid method for the production of fusion PCR products that can be used, generally without band pu

229 ugh primer design, we can create a series of PCR products that vary in size and contain only one rest

230 We obtained PCR products that were fluorescent enough to directly vi

231 electrolytes is presented with an unpurified PCR product, the tip surface electrostatically interacts

232 ING is dependent only on sufficient yield of PCR products, TILLING can be applied to most organisms.

233 tion (PCR), followed by hybridization of the PCR product to a fungus-specific oligonucleotide probe i

234 Additionally, the entire protocol from crude PCR product to an "electrosprayable" analyte solution re

235 -PCR) followed by sequencing analysis of the PCR product to differentiate Leishmania spp.

236 ctions from various sources by sequencing RT-PCR products to confirm gene structures.

237 SERS was used on the PCR products to detect mutations.

238 ligation-based assay is performed on the HF-PCR products to determine haplotypes.

239 nstructed by cloning alpha-and beta-tryptase PCR products to generate artificial templates.

240 med deligotyping, which hybridizes multiplex-PCR products to membrane-bound, highly specific oligonuc

241 ping assay involves reverse hybridization of PCR products to the capture spacers attached to nitrocel

242 t this streamlined protocol can be used with PCR products up to 1300 bp and had up to a 97% success r

243 DNA that was easily digested and yielded PCR products up to 8.5 kb in size was recovered from bro

244 polymorphisms were found in the tested cfp32 PCR products upon sequence analysis.

245 this technique to the detection of positive PCR products using an HIV-1 detection system.

246 for acquiring haplotypic sequences from long PCR products using simple, high-throughput techniques.

247 Analysis of PCR products using single-stranded conformational polymo

248 s have been used to directly detect specific PCR products utilizing the difference in adsorption of s

249 These were also the only strains to give PCR products (verifiable by sequencing) with degenerate

250 Direct detection of PCR product via hybridisation assay, would facilitate th

251 A PCR product was amplified from 27 of the hybrid lines, i

252 After amplification, each PCR product was analyzed on a WAVE microbial analysis sy

253 A 365-bp reverse transcriptase PCR product was generated from the VP7 gene segment usin

254 An appropriate PCR product was obtained in 79 of 80 reactions, while th

255 rae strain N16961 and found that no excision PCR product was produced, indicating that a functional c

256 romosome 8 (Acta1) so that the yield of each PCR product was proportional to the amount of its templa

257 The detected PRSV coat protein PCR product was sequenced and the nucleotide sequence sh

258 The PCR product was subjected to nucleotide sequencing.

259 The PCR product was subjected to temporal temperature gradie

260 single CpG methylation site when homogeneous PCR product was used as substrate.

261 lectrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma spec

262 modified primers for the direct detection of PCR products was applied to the genotyping of real patie

263 plify these alleles, and the analysis of the PCR products was performed by capillary electrophoresis.

264 ime PCR with bidirectional sequencing of the PCR products was used to resolve discrepancies, the sens

265 No PCR products were amplified from all nontarget control s

266 PCR products were analyzed by polyacrylamide gel electro

267 PCR products were cleaved with Nco I for the Asp299Gly p

268 The PCR products were cloned and sequenced to identify splic

269 PCR products were cloned into pGT3 efficiently without t

270 Multiplex PCR products were electrochemically detected in less tha

271 PCR products were generated from genomic DNA with primer

272 Single-stranded, fluorescence-tagged PCR products were hybridized to the microarrays at 15 de

273 PCR products were identified by DNA sequencing.

274 All PCR products were identified in gel-images and electroch

275 and multiplex assays with linear targets and PCR products were in the attomolar ranges.

276 DNA sequencing verified that these PCR products were indeed the result of specific amplific

277 PCR products were not observed for other dimorphic fungi

278 The PCR products were screened for mutations by single-stran

279 The PCR products were separated by capillary gel electrophor

280 The RT-PCR products were separated by gel electrophoresis and s

281 The fluorescently labeled PCR products were separated using capillary electrophore

282 ified according to the BIOMED-2 protocol and PCR products were sequenced directly.

283 The RT-PCR products were sequenced to confirm their identities.

284 n of desmosome-encoding genes was performed, PCR products were sequenced, and diseased tissue samples

285 The PCR products were then directly transformed into maize u

286 PCR products were then sized by automated capillary elec

287 CR) products, pyrosequencing, and cloning of PCR products were utilized to identify mutations in dhfr

288 synthetic DNA and polymerase chain reaction (PCR) products were accurately decoded, and a read length

289 Polymerase chain reaction (PCR) products were detected using this methodology, and

290 or H. pylori strains and did not produce any PCR products when other Helicobacter species and other b

291 CR, stutter patterns may appear in the final PCR product, which hinder us from accurate genotyping mi

292 The PCR product, which represents a pool of approximately 15

293 ten produce a high background of nonspecific PCR products, which can exclude the identification of th

294 combination is achieved by incubation of the PCR product with cells expressing lambda-Red proteins an

295 is step was also devised to dual-labeling of PCR products with biotin and 6-FAM, which are then easil

296 enosensor was suitable for discrimination of PCR products with different location of the complementar

297 me oxidase I, and 16S rRNA genes to generate PCR products with specific melting peaks that differenti

298 er amplification, the dU is excised from the PCR products with the USER enzyme leaving PCR products f

299 on by sequencing the pan-Plasmodium 18S rRNA PCR product, with correct identification in all cases.

300 PCR products (without any purification) amplified from A