1                                                             PCR was performed with a deoxynucleoside triphosphate mixture

2                                                Panbacterial PCR was performed with a nested pair of 16S rRNA gene primers

3                              To confirm gene expression, RT-PCR was performed with appropriate pairs of primers for rabbi

4                                                             PCR was performed with both fluid and tissue with IS6110-base

5                                                          RS-PCR was performed with conventional gel electrophoresis, and

6                                                             PCR was performed with degenerate oligonucleotide primers and

7                                                   Real-time PCR was performed with MMP and TIMP probes, and the results w

8               Microarray analysis and reverse transcriptase-PCR was performed with mRNA isolated from sorted diploid (2N)

9                                    RNA was isolated, and RT-PCR was performed with primers that flanked alternatively spl

10                                         The second round of PCR was performed with respective sense and antisense primers

11                                 To test this hypothesis, RT-PCR was performed with total RNA isolated from P. carinii f.

12 endent transcriptional activators involved in colonization, PCR was performed with V. cholerae chromosomal DNA and degene