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1 ion sequencing of the 16S rRNA gene as well as quantitative PCR was performed.
2             For both tests, DNA was extracted and real-time PCR was performed according to manufacturer's instructions.
3 incubated with recombinant IL-13 and gene expression by qRT-PCR was performed for collagen1A1 and TGF-beta1.
4                                                   Real-time PCR was performed for gene expression analysis.
5                                                   Real-time PCR was performed for genes integral to collagen synthesis an
6     Antibiotic susceptibility was determined, and multiplex PCR was performed for OXA-23-like, -24-like, -51-like, and -5
7                                      Real Time-quantitative PCR was performed for SERPINA3 transcript, and ACTB, RPL19, G
8                                      Quantitative real-time PCR was performed for TH1 and TH2 signature cytokines on tiss
9                                                          RT-PCR was performed for the purinergic receptors P2X(7), P2Y(1)
10                                   Quantitative real-time RT-PCR was performed for transcript levels.
11                                             Quantitative RT-PCR was performed on "dunked" sputum and NTS samples for infl
12                          Among Ab reactive cases, follow-up PCR was performed on 150 (88.2%), of which 102 (68.0%) were c
13                                    Standardized Aspergillus PCR was performed on 423 whole-blood pellets (WBP), 583 plasm
14 of the epidemiology of IDV, real-time reverse transcription-PCR was performed on a set of 208 samples from bovines with r
15                                               Lineage-based PCR was performed on a subset of samples.
16                                                          RT-PCR was performed on each transcript to determine the relativ
17                                    Standardized Aspergillus PCR was performed on plasma and serum samples concurrently ob
18                                                   Real-time PCR was performed on selected genes to confirm the results.
19 upon elution on the MagNA Pure LC instrument, and real-time PCR was performed on the Applied Biosystems 7500 Fast platfor
20                                                      Nested PCR was performed only in the samples from vascular lesions.
21                                      Real-time quantitative PCR was performed to assess cytokine gene expression.
22                                      Quantitative real-time PCR was performed to confirm gene expression.
23                                                          RT-PCR was performed to confirm some of the genes and bioinforma
24                                                          RT-PCR was performed to confirm the cdh23 mutation in Cdh23(nmf3
25 ion was measured by using ELISA, and real-time quantitative PCR was performed to detect PGE2 receptor expression.
26                                              Qualitative RT-PCR was performed to detect the mRNA of the alpha(2)-adrenerg
27                                                quantitative PCR was performed to determine factor XIII-A mRNA levels in a
28                                                Real-time RT-PCR was performed to determine FN and Coll IV mRNA levels, wh
29                                      Real-time quantitative PCR was performed to determine the expression of neurotrophin
30                                                   Real-time PCR was performed to examine the expression of stromal collag
31                                                       Fecal PCR was performed to identify ETEC and EAEC in DNA extracted
32  by enzyme-linked immunosorbent assay, whereas quantitative PCR was performed to measure interleukin-6 (IL-6, a pro-infla
33                                      Quantitative real-time PCR was performed to measure the expression of tumor necrosis
34                                        Quantitative genomic PCR was performed to study copy number variation at the miR-1
35                                      Quantitative real-time PCR was performed to validate a subset of identified changes.
36                                                         qRT-PCR was performed to validate the expression of the selected
37 ies were taken and total RNA extraction, cDNA synthesis and PCR was performed using 10 candidate HKG.
38                                      Quantitative real-time PCR was performed using leaf RNA extracted from three wheat c
39                                                          RT-PCR was performed using primers specific for murine IL-1alpha
40                                                          RS-PCR was performed with conventional gel electrophoresis, and
41 at code identified protein, quantitative real time PCR (qRT-PCR) was performed.
42 acterial repetitive intergenic consensus sequence PCR (ERIC-PCR) was performed.
43 (MFC) and real-time quantitative polymerase chain reaction (PCR) was performed at the end of induction and at ~3-month in
44 tative reverse transcription polymerase chain reaction (qRT-PCR) was performed in 2 groups of BE patients who either deve
45                      One-step reverse transcriptase PCR (RT-PCR) was performed in picoliter drops with primers that ident
46          Polymerase chain reaction for respiratory viruses (PCR) was performed on 1021 specimens obtained from 898 childr
47                           Nested polymerase chain reaction (PCR) was performed on all samples as the reference standard.
48 tative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to assess the messenger RNA (mRNA) express
49                          Digital polymerase chain reaction (PCR) was performed to measure HLA-DR RNA transcripts in conju
50                                    Quantitative RT-PCR (qRT-PCR) was performed with primers positioned within nonspliced

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