ライフサイエンスコーパス: PCR

1 PCR protocols were designed to target 5 sequences unique

2 PCR screening of ETEC isolates revealed that 8.6% (n = 1

3 PCR targeting the 16S rRNA-encoding gene and chaperonin-

4 PCR-CI was defined as molecular diagnostic evidence of i

5 PCR-HRM analysis of DNA extracts from Giemsa-stained thi

6 Out of 1392 PCR amplified samples, a single sample was Pfhrp2 negati

7 d merging, barcode demultiplexing, 5' and 3' PCR primer matching, and duplicate reads collapsing.

8 sent research has shown the feasibility of a PCR-CE barcode assay to detect adulteration in olive oil

9 All PCR-proven P. malariae monoinfections in Israeli travele

10 were only amplifiable from PMEZ DNA and all PCR generated phnJL clones matched those of the Pseudomo

11 and most interestingly, that SFM4-3 can also PCR amplify these modified oligonucleotides.

12 Although PCR is highly effective in vitro, methods that can funct

13 The distribution of load among PCR-positive participants was compared between microbiol

14 ficity of 96.7% (95% CI, 93.0 to 98.8%), and PCR-HRM analysis of pellet DNA had a sensitivity of 100.

15 We assessed antigen- and PCR-based RSV reports submitted to the National Respirat

16 taxa, which would affect amplicon based and PCR free methods alike.

17 ng and detect cross-sample contamination and PCR amplification bias.

18 r enhancing the flexibility of quick DNA and PCR detection.

19 have been observed with regard to ELISA and PCR methods.

20 tests (means of 9.5 and 6.8 positive GM and PCR samples, respectively), whereas both possible IA cas

21 dge that included both sample processing and PCR amplification functions was loaded with all reagents

22 l protein A (spa) typing, SCCmec typing, and PCR-based assays were used to detect mecA, mecC, vanA, P

23 f 36 patients with clinically diagnosed ARN, PCR-positive for herpes simplex virus or varicella zoste

24 Nucleic acid amplification tests such as PCR have significantly accelerated the detection of spec

25 Targeted deep-sequencing confirmed AS-PCR findings, and identified an additional BTK(Cys481Tyr

26 ture, galactomannan antigen, and aspergillus PCR are useful tests.

27 respiratory virus infection, and Aspergillus PCR positivity were all significant risk factors for dev

28 , with clinical risk factors and Aspergillus PCR results, subjected to multilogistic regression analy

29 t sequencing, locked nuclei acid (LNA)-based PCR, and immunohistochemistry.

30 The odds ratios for being PCR positive was 7.4 (95% confidence interval, 2.8-19.9)

31 Both PCR-based and MLVA methods are limited in discriminating

32 0.2%) were positive by RDT and 123 (2.3%) by PCR.

33 on of their promoter regions was analyzed by PCR and pyrosequencing.

34 displayed HCMV DNA detection in the BALF by PCR, whereas other infectious agents were undetectable.

35 e IncX4 plasmids were fully characterized by PCR-RFLP.

36 statistical benefit for eyes complicated by PCR, and IC antibiotic prophylaxis should be strongly co

37 reas both possible IA cases were detected by PCR.

38 One hundred sixty children were examined by PCR and serologically studied at 0-6, 9 and 12 months an

39 DNA synthesis (TLS) polymerase, followed by PCR amplification and next-generation sequencing (NGS) t

40 al masses, previously formally identified by PCR amplification and sequencing of internal transcribed

41 he participants with confirmed influenza (by PCR), 28 (67%) of 42 in the plasma plus standard care gr

42 ubular epithelial cells were investigated by PCR and enzyme-linked immunosorbent assay in an in vitro

43 compared the results with those obtained by PCR-cloning-sequencing (PCR-CS).

44 infections were detected retrospectively by PCR (n = 55), and (3) uninfected children (RDT/PCR negat

45 positive for the five gene alleles tested by PCR; however, 5 to 36% of field isolates were also posit

46 n with RTI, the median HMPV shedding time by PCR was 13 days (range, 6-28 days), but all were culture

47 ubstitutions were created in the xylanase by PCR, and the mutants were expressed in Pichia pastoris.

48 xFP, and waDP, respectively, using a chicken PCR array comprising 27 AhR-related genes.

49 ells in processed products, and conventional PCR-based methods are laborious and time-intensive.

50 Unlike conventional PCR, RT-PCR detected pork DNA in nine processed food sam

51 que molecular identifiers (UMI) that correct PCR bias.

52 eads via an iterative approach that corrects PCR and sequencing errors and removes PCR-mediated recom

53 Digital PCR (dPCR) is an important new tool for use in the clini

54 gorithm to multiplexed droplet-based digital PCR data sets in both EvaGreen and probes-based schemes,

55 Here, Droplet Digital PCR (ddPCR) and qPCR platforms were directly compared fo

56 cies of L1s were assessed by droplet digital PCR or Taqman genotyping.

57 Standard data analysis pipelines for digital PCR estimate the concentration of a target nucleic acid

58 s with real-time quantitative PCR or digital PCR to determine antimicrobial susceptibility.

59 bias by integrating functions to run digital PCR.

60 e-editing were confirmed by cell genomic DNA PCR and fluorescent marker expression analysis.

61 Compared with serology, DNA PCR had high clinical sensitivity (100%) but, because of

62 e technique developed here was 0.00001ng DNA/PCR.

63 However, in vitro recombination during PCR amplification could not be excluded.

64 emistry, the Luminex Multiplex assay, ELISA, PCR, and immunoblotting and linked to the presence of EE

65 e recovery of the partner genes via emulsion PCR.

66 egions using the RainDance target enrichment PCR method and sequenced the products using the MiSeq NG

67 ese artificial linkages by primer extension, PCR, and deep sequencing reveals that a subtle interplay

68 droplet then becomes a reaction chamber for PCR, which through the use of fluorochrome labelled prob

69 ears represent a potential source of DNA for PCR testing to confirm Plasmodium infections or for epid

70 aditional DNA amplification protocols (e.g., PCR) with an improved limit of detection.

71 atic newborns (6.8%) had a positive CSF hCMV-PCR.

72 on suggests that implementation of rapid HSV PCR testing can decrease turnaround times and the durati

73 The adoption of criteria to screen HSV PCR tests in CSF represents a cost-effective approach.

74 to account for these errors when identifying PCR duplicates.

75 Chromatin immunoprecipitation-PCR detected increased ETS1 and histone 3 lysine 4 trime

76 by spiking B. anthracis DNA into individual PCR mixtures and B. anthracis CFU into human blood.

77 esults with those from the conventional ITS2-PCR approach.

78 ryos through analysis of fluorophore-labeled PCR amplicons covering the nuclease target site by capil

79 In the DNA-based method, PCR amplification of mitochondrial D loop gene using spe

80 end-point fluorescence of the parallel micro-PCR reactions, using an automated hard threshold.

81 Compared to microscopy, PCR-HRM analysis of smear DNA had a sensitivity of 93.0%

82 ental conditions and identified by molecular PCR assay based on the ITS-5.8S rDNA.

83 TB-DzT combines a multiplex PCR with single nucleotide polymorphism (SNP) detection

84 aph ID/R blood culture panel (BCP) multiplex PCR assay (Great Basin Scientific, Salt Lake City, UT) f

85 Firstly, multiplex PCR analysis indicated that the novel strain belongs to

86 Finally, a novel multiplex PCR assay, based on random amplified polymorphic DNA (RA

87 Our method relies on multiplex PCR for targeted enrichment of viral genomes from sample

88 es an authentication protocol with multiplex PCR for three species of snappers (Lutjanus purpureus, L

89 Simple, multiplexed, PCR-based barcoding of DNA for sensitive mutation detect

90 novative strategy consisting of multiplexing PCR, targeted sequencing and computational analysis.

91 Of the treated mothers, 92.1% had negative PCR results, compared with 32.2% of untreated mothers.

92 A new three-phase, double-nested PCR approach allowed robust melting temperature analysis

93 eropositive donor samples by in-house nested PCR and quantitative real-time PCR assays, respectively.

94 d for sample processing and multiplex nested PCR.

95 tive polymerase chain reaction (PCR) (nested PCR targeting the cytochrome b gene) and quantitative PC

96 Compared with nested PCR, the sensitivity, specificity, positive predictive v

97 Most current non-PCR detection methods rely on linear signal amplificatio

98 In this study, a novel PCR-RFLP protocol was developed as a tool to authenticat

99 nosis are increasing despite availability of PCR testing, indicating the need for strategies to impro

100 A combination of PCR tests for vlhA3.04a, vlhA3.05, and mg0359 was able t

101 to elucidate the source and epidemiology of PCR ribotype 265, primarily found in children.

102 cebo recipients, the respective incidence of PCR-CI and SDI was 5.6% and 35.0% in HIV-uninfected wome

103 is step was also devised to dual-labeling of PCR products with biotin and 6-FAM, which are then easil

104 Hotspots by RDT were predictive of PCR/microscopy at the moderate setting, but not at the 2

105 Surprisingly, a reduction of PCR cycles did not have a strong effect on amplification

106 ion was associated with an increased risk of PCR (P = .037), with an odds ratio of 1.66.

107 Eyes with previous IVI have a higher risk of PCR.

108 In this study, the sequencing of PCR amplicons generated using primers targeting either k

109 A set of PCR cassettes facilitates the introduction of recombinan

110 This procedure eliminates (i) the use of PCR-inhibiting reagents (e.g., chaotropic salts and alco

111 Diagnosis of opisthorchiasis was based on PCR performed on stool samples.

112 Routine histopathology studies and/or PCR for refractory colitis patients are suggested to dia

113 ate culture or PCR, pleural fluid culture or PCR, blood culture, and immunofluorescence for P. jirove

114 gen identified from lung aspirate culture or PCR, pleural fluid culture or PCR, blood culture, and im

115 were serotyped with the Quellung reaction or PCR.

116 Among our PCR-specific approaches, the most sensitive and consiste

117 omates the design of primers for overlapping PCR.

118 We enrolled cases with microscopy-positive, PCR-confirmed malaria who presented to two primary refer

119 C9orf72 repeat expansion with repeat-primed PCR (RP-PCR).

120 Using quantitative PCR (q-PCR), immunostaining and patch clamp recordings of membr

121 qRT-PCR assay detected higher miR-214 expression in the plas

122 qRT-PCR assay indicated that compound 1 and DBL exposure up-

123 qRT-PCR of pathogen miRNAs isolated from extracellular vesic

124 qRT-PCR-based measurements revealed multifold inaccuracies a

125 RNA-seq and qRT-PCR expression analysis showed that overexpression of Se

126 RNA sequencing and qRT-PCR revealed that arginine biosynthesis genes (argR, arg

127 blotting, immunofluorescence, ELISA and qRT-PCR, we investigated the production of transthyretin by

128 aforementioned stresses was confirmed by qRT-PCR analysis in distinct Arachis genotypes, whilst in si

129 (emx2, lhx2, and hopx), was validated by qRT-PCR and immunostaining in brain sections.

130 going bariatric surgery were analyzed by qRT-PCR for expression of WNT/PCP genes.

131 ratio was determined by SDS PAGE and by qRT-PCR in ex-vivo bone explants.

132 Validation analysis by qRT-PCR showed significant upregulation of only miR-455-3p (

133 ferent S. mussotii tissues, validated by qRT-PCR, and compared with the homologous genes from S. japo

134 ariations by qPCR, RNA concentrations by qRT-PCR, and protein concentrations by immunoblotting.

135 ass switch recombination was measured by qRT-PCR.

136 Confirmatory qRT-PCR analysis demonstrated good correlation with SnoRNASe

137 quantitative reverse transcription-PCR (qRT-PCR) and microarrays have shown a significant transient

138 noRNASeq and real-time quantitative PCR (qRT-PCR) we demonstrate snoRNA expression levels in murine a

139 Using quantitative real-time PCR (qRT-PCR) we have measured the gene expression profiles of ea

140 ction (RT-PCR), and quantitative RT-PCR (qRT-PCR), and the proinflammatory cytokines interleukin 1bet

141 quantitative reverse-transcriptase PCR (qRT-PCR), immunofluorescence, and Luminex technology.

142 idated using quantitative real-time PCR (qRT-PCR).

143 transcription polymerase chain reaction (qRT-PCR) amplification of miRNA extracted directly from 500

144 d correlation between the DRELFA and the qRT-PCR over a 50-fold concentration range.

145 We used qRT-PCR, Western blotting, ELISA, and ChIP (chromatin immuno

146 methylation changes were confirmed using qRT-PCR and qMSP methods.

147 ressed and secreted) were assessed using qRT-PCR.

148 n and wound healing assays together with qRT-PCR determination of epithelial-to-mesenchymal transitio

149 Quantitative PCR and immunohistochemistry showed that HOXB13 gene and

150 Quantitative PCR was used to compare bacterial load and Illumina MiSe

151 Through use of a quantitative PCR assay for HHpgV-1, infection was also detected in 17

152 were compared by microarray and quantitative PCR analyses.

153 istologic, flow cytometric, and quantitative PCR analysis.

154 ting the cytochrome b gene) and quantitative PCR as reference standards.

155 alyzed using flow cytometry and quantitative PCR.

156 f 48 that were PCV3 positive by quantitative PCR (qPCR), with 60% of a subset also testing positive f

157 d after 4 weeks and analyzed by quantitative PCR and 16S rRNA sequencing.

158 CFHR3 and CFHR1 gene copies by quantitative PCR and collected clinical and biologic data by reviewin

159 BDNF mRNA levels, assessed by quantitative PCR and in situ hybridization at 1, 4, 7, and 15 days af

160 ke receptor (TLR) expression by quantitative PCR, and cytokine secretion after stimulation with mitog

161 macrophages was investigated by quantitative PCR, immunoblotting and flow cytometry.

162 were measured in whole blood by quantitative PCR.

163 ospinal fluid were amplified by quantitative PCR.

164 red to that with a conventional quantitative PCR (qPCR) assay and blood culture.

165 ear accumulation, and data from quantitative PCR analysis closely recapitulated the EdU results.

166 LISA, cytometric bead array, or quantitative PCR.

167 e we describe a novel real-time quantitative PCR (qPCR) approach based on the SYBR green technology (

168 Carba-R test, a rapid real-time quantitative PCR (qPCR) assay that detects five families of carbapene

169 Using SnoRNASeq and real-time quantitative PCR (qRT-PCR) we demonstrate snoRNA expression levels in

170 tain antibiotics with real-time quantitative PCR or digital PCR to determine antimicrobial susceptibi

171 d by using ELISA, and real-time quantitative PCR was performed to detect PGE2 receptor expression.

172 d to mRNA extraction, real-time quantitative PCR, enzyme-linked immunosorbent assay, and immunohistoc

173 were determined using real-time quantitative PCR.

174 Real Time-quantitative PCR was performed for SERPINA3 transcript, and ACTB, RPL

175 Here, ddPCR was compared to quantitative PCR (qPCR) and pyrosequencing.

176 viral RNA reverse transcription-quantitative PCR (RT-qPCR)-based assays and from outgrowth assay read

177 Using quantitative PCR (q-PCR), immunostaining and patch clamp recordings o

178 activity were assessed by using quantitative PCR and the telomere repeat amplification protocol from

179 ton's gene of genomic DNA using quantitative PCR.

180 antibodies and MCPyV DNA using quantitative PCR.

181 Upon validation with quantitative PCR, we studied the association of the top 5 differentia

182 We used quantitative PCRs and a telomere-sequence to single-copy-gene-sequenc

183 he 24 human chromosomes that allow for rapid PCR assays capable of capturing the genomic landscape of

184 16S rDNA PCR analysis reveals the presence of bacterial DNA in in

185 R (n = 55), and (3) uninfected children (RDT/PCR negative) (n = 434).

186 using qualitative polymerase chain reaction (PCR) (nested PCR targeting the cytochrome b gene) and qu

187 t sera as well as polymerase chain reaction (PCR) and larval identification of the meat samples was c

188 Polymerase chain reaction (PCR) and other molecular assays have demonstrated an ass

189 Quantitative polymerase chain reaction (PCR) and reverse-transcription (RT) PCR were applied to

190 volving real-time polymerase chain reaction (PCR) assays that facilitate direct analysis of a single

191 was quantified by polymerase chain reaction (PCR) cycles in blood/ urine.

192 umococcus by lytA polymerase chain reaction (PCR) in blood had poor diagnostic accuracy for diagnosin

193 both culture and polymerase chain reaction (PCR) in children.

194 eened for cCMV by polymerase chain reaction (PCR) in saliva.

195 al fluid (CSF) by polymerase chain reaction (PCR) is a marker of central nervous system (CNS) involve

196 Routine polymerase chain reaction (PCR) ribotyping and multiple-locus variable-number tande

197 le using specific polymerase chain reaction (PCR) sequencing and culture.

198 nd a quantitative polymerase chain reaction (PCR) test for JAK2/V617F was negative.

199 Polymerase chain reaction (PCR) testing of aqueous or vitreous humor was positive f

200 eq) and real-time polymerase chain reaction (PCR) were used to examine transcriptional dysregulation.

201 echniques such as polymerase chain reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization

202 for quantitative polymerase chain reaction (PCR), RNA sequencing, and comparison of the transcriptom

203 real-time immuno-polymerase chain reaction (PCR), to parasitemia limits of 0.02 parasite/microL and

204 ite prevalence by polymerase chain reaction (PCR)- and the prevalence of antibodies to two P. falcipa

205 and quantitative polymerase chain reaction (PCR).

206 rse transcription polymerase chain reaction (PCR).

207 rse transcription polymerase chain reaction (PCR).

208 lony-counting and polymerase chain reaction (PCR).

209 nasal samples by polymerase chain reaction (PCR).

210 X, DMP1, and Wnt3a, was observed by realtime PCR.

211 rrects PCR and sequencing errors and removes PCR-mediated recombinant sequences (chimeras).

212 repeat expansion with repeat-primed PCR (RP-PCR).

213 most common spider species using targeted RQ-PCR to quantify the potential efficiency of spiders as a

214 the real-time reverse transcription-PCR (rRT-PCR) reference method, the Aptima ZIKV assay detected ZI

215 eaction (PCR) and reverse-transcription (RT) PCR were applied to nasopharyngeal swab (NPS) samples fr

216 RT-PCR demonstrated that 10 microM YC-1 reduced hypoxia-ind

217 Using FACS and RT-PCR, we examined the phenotype of generated IgE(+) cells

218 uzi deoxyribonucleic acid was detected by RT-PCR at 30, 60, 90, 120, 150, 180, and 360 days.

219 siently transfected cells was examined by RT-PCR, western blot analysis, and immunohistochemistry.

220 ene expression analyses were performed by RT-PCR.

221 situ hybridization, immunohistochemistry, RT-PCR, northern blot and western blot experiments.

222 Consistent with this notion, RT-PCR of lymphocyte cell lines from one of the kindreds sh

223 rase chain reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization have also been proposed

224 Unlike conventional PCR, RT-PCR detected pork DNA in nine processed food samples [ch

225 ve real-time polymerase chain reaction (Q-RT-PCR).

226 chain reaction (RT-PCR), and quantitative RT-PCR (qRT-PCR), and the proinflammatory cytokines interle

227 Quantitative RT-PCR and ELISA were used to determine transcript and secr

228 in independent LSE samples (quantitative RT-PCR and Western blotting) and in normal and AE skin biop

229 were confirmed by real-time quantitative RT-PCR, and DNA methylation of their promoter regions was a

230 on flow cytometry, real-time quantitative RT-PCR, and mass spectrometry were used to characterize EV

231 ology, immunohistochemistry, quantitative RT-PCR, ELISA, and flow cytometry.

232 ung tissue was determined by quantitative RT-PCR.

233 BER genes were assessed by quantitative RT-PCR.

234 -transcription polymerase chain reaction (RT-PCR) analysis in 17 cases and by serology in 6 cases.

235 -transcription polymerase chain reaction (RT-PCR) testing was not performed, resulting in a missed di

236 transcription-polymerase chain reaction (RT-PCR) to analyse the effects of these treatments on nucle

237 -transcription polymerase chain reaction (RT-PCR), and quantitative RT-PCR (qRT-PCR), and the proinfl

238 with real-time polymerase chain reaction (RT-PCR)-confirmed EVD were enrolled retrospectively in 5 Eb

239 Among RIV4 recipients, the RT-PCR-confirmed influenza attack rate was 2.2% (96 cases a

240 In comparison to the standard real-time RT-PCR method, the MeVA RT-qPCR showed 99.5% specificity fo

241 tbreaks to detect CV-A6 and CV-A10, using RT-PCR.

242 The primary end point was RT-PCR-confirmed, protocol defined, influenza-like illness

243 gate the risk of posterior capsular rupture (PCR) during cataract surgery in eyes with previous intra

244 We performed bisulfite sequencing PCR of genomic DNA isolated from HBV-related HCCs and HB

245 th those obtained by PCR-cloning-sequencing (PCR-CS).

246 lastic material and performed multiplexed SP-PCR directly on top of the SAF microlens array.

247 utilizing KASP (Kompetitive Allele-Specific PCR) genotyping technology to create scarless isogenic c

248 By using methylation-specific PCR and bisulfite sequencing we demonstrate that miR-663

249 Species-specific PCR and real-time PCR with EvaGreen dye targeting the IT

250 previously from the same samples by standard PCR cloning and Sanger sequencing, and showed that both

251 tion with a bridge linker, Tn5 tagmentation, PCR amplification and high-throughput sequencing.

252 Archaea-targeted PCR sequencing and metagenomics confirmed M. oralis alon

253 ecies per product from the PGM platform than PCR-CS.

254 rucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-

255 s polymerase chain reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization have also been p

256 s were measured using quantitative real time PCR and multiplex assay.

257 Using quantitative real-time PCR (qRT-PCR) we have measured the gene expression profi

258 s was validated using quantitative real-time PCR (qRT-PCR).

259 Real-time PCR and ELISA analyses showed that ER stress induced a p

260 esting for Ebola virus was done by real-time PCR and for malaria by a rapid diagnostic test.

261 Genetic markers were determined by real-time PCR and, with clinical risk factors and Aspergillus PCR

262 A normalised real-time PCR approach was also proposed in the range of 0.1-20% (

263 The emergence of real-time PCR assays utilizing allele-specific molecular detection

264 -house nested PCR and quantitative real-time PCR assays, respectively.

265 M. pneumoniae was detected by real-time PCR in 175 (5.8%) specimens.

266 The fungus was detected using real-time PCR in 26 (8.6%) specimens across the period of collecti

267 patch-clamp electrophysiology and real-time PCR in MNCs in sham and renovascular hypertensive (RVH)

268 Real-time PCR measurements of SK channel subunits mRNA in supraopt

269 an DNA (8.6 copies), with adequate real-time PCR performance parameters, regardless of the soybean ma

270 [Stratagene-Agilent] and the 7500 real-time PCR system [ABI Life Technologies]).

271 In this work, two real-time PCR systems based on the EvaGreen dye and a TaqMan probe

272 we used cycle threshold values of real-time PCR to guide the choice of protocols for SNP amplificati

273 ted empirically using quantitative real-time PCR to measure gene expression.

274 al 7 samples that were negative by real-time PCR were positive with T2MR.

275 er D) was lower in NF1-iN cells by real-time PCR with 12 sex-mixed samples.

276 Species-specific PCR and real-time PCR with EvaGreen dye targeting the ITS region of Cartha

277 This multiplex real-time PCR, based on the dual-labeled probe strategy, guarantee

278 t of 21 clinical samples tested by real-time PCR, only 1 was positive and 13 were negative with agree

279 MUC4beta was evaluated by means of real-time PCR, Western blotting, and immunohistochemistry.

280 re screened for colonization using real-time PCR.

281 ession of key anabolic proteins by real-time PCR.

282 the model foods was determined by real-time PCR.

283 s quantified by using quantitative real-time PCR.

284 of extracellular flux analysis and real-time PCR.

285 on H4R and RANKL was determined by real-time PCR.

286 c assays (ELISA), and quantitative real-time PCR.

287 ore, the LC-MS/MS and quantitative real-time-PCR analysis followed by inhibitor and antibody-blocking

288 re, have emerged as promising alternative to PCR and greatly simplify the implementation of amplifica

289 y pathways being impacted, two avian ToxChip PCR arrays-chicken and double-crested cormorant-were uti

290 firmed by quantitative reverse transcriptase PCR in infected and uninfected gastric mucosa obtained f

291 ues are measured using reverse transcriptase PCR, microarray analysis or high-throughput sequencing.

292 T. pallidum in CSF by reverse transcriptase PCR, or (iii) new vision loss or hearing loss.

293 ges using quantitative reverse-transcriptase PCR (qRT-PCR), immunofluorescence, and Luminex technolog

294 ies using quantitative reverse transcription-PCR (qRT-PCR) and microarrays have shown a significant t

295 pared to the real-time reverse transcription-PCR (rRT-PCR) reference method, the Aptima ZIKV assay de

296 for flow cytometry and nasal swabs for viral PCR were performed at enrollment and during convalescenc

297 diatric admissions, 3.9% (n = 274/6968) were PCR-positive, including 61.7% (n = 37/60) of those with

298 this challenging diagnosis, especially when PCR is delayed, shows negative results, or is not availa

299 S libraries from as little as 20 pg DNA with PCR error correcting capabilities, and capture target se

300 e used multivariate logistic regression with PCR-confirmed influenza infection as the outcome and vac