ライフサイエンスコーパス: PHD finger

1 e C terminus of RAG2 contains a noncanonical PHD finger.

2 The ASH1 protein contains a SET domain and a PHD finger.

3 that is dependent on histone binding by the PHD finger.

4 ylase activity, a property that requires the PHD fingers.

5 l role in determining the selectivity of the PHD fingers.

6 of the histone and non-histone targeting by PHD fingers.

7 one 3 (H3K4me3) through a plant homeodomain (PHD) finger.

8 istones, by one of Aire's plant homeodomain (PHD) fingers.

9 BRPF1 (bromodomain PHD finger 1) is a core subunit of the MOZ histone acety

10 ING2 contains a plant homeodomain (PHD) finger, a motif common to many chromatin-regulatory

11 mimic disease-causing mutations in the hATRX PHD finger, abolish repression.

12 a conserved tryptophan residue in the RAG-2 PHD finger abolished binding to H3K4me3 and greatly impa

13 in the non-core region and suggests that the PHD finger adopts two distinct states.

14 The removal of PHD fingers affected neither binding nor mutual Jade-1-H

15 ough mutations in either the first or second PHD finger allow for Rpd3S complex formation, the assemb

16 dition of the PHD finger domain or the third PHD finger alone into MLL-ENL blocks the hematopoietic s

17 milar preferences to those displayed by each PHD finger alone.

18 ld increase in affinity compared with either PHD finger alone.

19 e, the first report that deregulation of the PHD finger, an 'effector' of specific histone modificati

20 s including a C-terminal SET domain, central PHD fingers, an N-terminal DNA-binding homology, and two

21 InsP-binding region of ING2 (consisting of a PHD finger and a polybasic region) revealed a number of

22 We demonstrate that the tandem PHD finger and bromodomain of KAP-1, an arrangement ofte

23 nteraction of KAP1 with HP1 and on an intact PHD finger and bromodomain of KAP1, suggesting that thes

24 on in the linker region between MLL1's third PHD finger and bromodomain.

25 ontaining the HMG box and hath region plus 4 PHD fingers and a SET domain.

26 ocalization signal (NLS), a SAND domain, two PHD fingers and four nuclear receptor targeting motifs.

27 ent domain that includes the CXXC domain and PHD fingers and is controlled by direct interactions wit

28 MLL1 fusion proteins lack the PHD fingers and require prebinding of a wild-type MLL1 c

29 hy MLL translocation breakpoints exclude the PHD fingers and suggest a possible role for these domain

30 s consistently delete the plant homeodomain (PHD) fingers and more carboxyl terminal MLL sequences.

31 ative heterochromatin localization domain, a PHD finger, and a bromodomain, prevalent in factors invo

32 region with an acidic stretch, a WAKZ motif, PHD fingers, and bromodomain.

33 Hence, the PHD finger appears to negatively regulate self-acetylati

34 n of the aromatic H3K4me-binding site of the PHD fingers appears to have no effect.

35 noprecipitation experiments reveal that both PHD fingers are required for binding to H3K14ac in vivo

36 Together, these data identify the PHD finger as a novel and functionally important domain

37 Together, our data identify the PHD finger as a phosphoinositide binding module and a nu

38 Our findings call attention to the PHD finger as a previously uncharacterized chromatin-bin

39 itate the functional characterization of new PHD fingers, as well as other protein families, solely b

40 We show that human and Drosophila Pygo PHD fingers associate with their cognate HD1 domains fro

41 Pygopus contains a PHD finger at its C terminus, a motif often found in chr

42 of immunodeficiencies are located within the PHD finger, at either zinc-coordinating residues or resi

43 ectroscopic approaches to show that the Set3 PHD finger binds di- and trimethylated states of H3K4 wi

44 The ING2 PHD finger binds H3K4me3, a histone mark that is associa

45 solution crystal structure of the mouse RAG2 PHD finger bound to H3K4me3 reveals the molecular basis

46 nsferase domain and the adjacent bromodomain/PHD finger (bromo/PHD) region of the transcriptional coa

47 a/TRIM33/Ectodermin and demonstrate that its PHD finger-bromodomain constitutes a multivalent histone

48 biquitinate its substrate Smad4 requires its PHD finger-bromodomain, as does its transcriptional repr

49 ribe a dual inhibitor of the bromodomain and PHD finger (BRPF) family member BRPF2 and the TATA box b

50 MLL5 includes a SET domain and a single PHD finger, but lacks A-T hooks and methyltransferase ho

51 Deletion of the PHD finger, but not the bromodomain, impaired the abilit

52 The three PHD finger cassette, one of the highly conserved domains

53 igenome reader domain families (Bromodomain, PHD finger, Chromodomain, MBT, PWWP and Tudor).

54 Deletion of the two PHD fingers completely abolished Jade-1 transcriptional

55 CHD4 contains tandem plant homeodomain (PHD) fingers connected by a short linker, the biological

56 e provide evidence on the genomic scale that PHD fingers constitute a general class of effector modul

57 ining protein 24) and BRPF1 (bromodomain and PHD finger containing protein 1) are involved in the epi

58 URF was disabled by silencing of bromodomain PHD-finger containing transcription factor (BPTF), the l

59 ial readout for selective recruitment of the PHD finger-containing components of chromatin remodeling

60 he histone deacetylase HDAC1, Mrg15, and the PHD finger-containing Pf1 and show that this complex pla

61 Bromodomain and PHD finger-containing protein 1 (BRPF1) is a multivalent

62 Bromodomain- and PHD finger-containing protein 1 (BRPF1) is a multivalent

63 BRPF1 (bromodomain- and PHD finger-containing protein 1) is a unique chromatin r

64 Cti6 is a PHD finger-containing protein that has been shown to par

65 volutionary conserved genes encoding nuclear PHD finger-containing proteins implicated in a variety o

66 The BRPF (bromodomain and PHD finger-containing) family are scaffolding proteins i

67 Here we show that a plant homeodomain (PHD) finger-containing protein, VIN3-LIKE 1 (VIL1), part

68 (also known as PHF21A), a plant homeodomain (PHD) finger-containing protein.

69 Plant homeodomain (PHD) finger-containing proteins are implicated in fundam

70 s essential for viability and that the first PHD finger contributes to the preferred binding of PHD1-

71 Structural modeling of PHD fingers demonstrates a conserved mechanism for recog

72 ilarity to one another within the C-terminal PHD finger domain and also contain an additional N-termi

73 the transcription repression activity to the PHD finger domain of the chick Pcl2 protein.

74 Finally, the addition of the PHD finger domain or the third PHD finger alone into MLL

75 cells is critically dependent on a conserved PHD finger domain, suggesting that Pcl2 might function t

76 se screens identified the plant homeodomain (PHD)-finger domain protein PHF5A as differentially requi

77 lt shows that the binding specificity of the PHD-finger domain of VIN3 plays a role in mediating a pr

78 alteration in the binding specificity of the PHD-finger domain of VIN3 results in a hypervernalizatio

79 VIN3 encodes a PHD-finger domain that binds to modified histones in vit

80 To date, about 15 PHD finger domains have been structurally characterized,

81 PHD finger domains in viral proteins and in the cellular

82 auxin responses through the action of their PHD finger domains.

83 (NLS) sequences, and four plant homeodomain (PHD) finger domains.

84 Msc1 and RBP2 each possess three PHD fingers, domains commonly found in proteins that inf

85 H3 tail structure in complex with the double PHD finger (DPF) of the lysine acetyltransferase MOZ/MYS

86 ontaining C terminus of BRM binds to the CBP PHD finger, enhances PHD binding to histone H3, and enha

87 Binding studies establish that the BPTF PHD finger exhibits a modest preference for K4me3- over

88 ventional PHD finger followed by an extended PHD finger exists in the mammalian AF10 protein, among a

89 A specific module containing a conventional PHD finger followed by an extended PHD finger exists in

90 timulated 500-fold by Slx4, and requires the PHD finger for activity in vitro and in vivo.

91 on differences in binding affinities of the PHD fingers for H3K4me and the methylation state of the

92 d protein that is homologous to the multiple PHD fingers found in the N-terminal regions of mammalian

93 The protein contains a SET domain, a PHD finger, four AT hooks, and a region with homology to

94 3 ligases, reporting for the first time that PHD fingers from a nuclear protein exhibit E3 ubiquitin

95 l-characterized CBDs: the plant homeodomain (PHD) finger from ING2 and the chromodomain from heteroch

96 The PHD finger functions as an epigenetic reader that binds

97 A line of evidence suggests that the PHD finger functions in chromatin remodeling and protein

98 rations to sites that differed from both the PHD finger fusion-directed and LEDGF-directed integratio

99 Similar PHD finger-H3 tail-binding properties were recently repo

100 Furthermore, our data suggest that the PHD finger has a role in the recruitment of p300 to chro

101 The dysregulation of PHD fingers has been implicated in several human disease

102 Insertion of only the first and second PHD fingers has no such effect.

103 A few PHD fingers have recently been found to also associate w

104 that contain one or more plant homeodomain (PHD) fingers have been implicated in the regulation of c

105 Plant homeodomain (PHD) fingers have emerged as one of the largest families

106 on as to how BCL9 co-factors binding to Pygo PHD fingers impact indirectly on their histone binding a

107 2.0 A resolution structure of the mouse ING2 PHD finger in complex with a histone H3 peptide trimethy

108 To evaluate the role of Aire's PHD finger in MECs on a global scale in vivo, we complem

109 methylated at lysine 4 (H3K4me3) by the ING4 PHD finger in mediating ING4 gene expression and tumor s

110 Here we show that inclusion of the PHD fingers in the MLL fusion protein MLL-AF9 blocked im

111 talizing MLL fusion protein, the loss of the PHD fingers, in combination with the gain of the activat

112 d substitutions in either the bromodomain or PHD finger, including ones that mimic disease-causing mu

113 s to investigate the interaction of the ING3 PHD finger (ING3PHD) with the active transcription mark

114 e, expression of the fusion protein with the PHD finger insertion mediates the down-regulation of the

115 Further, we demonstrate that the ING2 PHD finger interacts with PtdIns(5)P in vivo and provide

116 Here, we classify PHD fingers into different groups based on the analysis

117 The function of the PHD fingers is obscure and their specific role in transf

118 t recognition of the histone H3 tails by the PHD fingers is required for repressive activity of the C

119 ur study suggests that a common function for PHD fingers is to transduce methyl-lysine events and she

120 ne 3 (H3)-tails by Aire's plant homeodomain (PHD) finger is essential for Aire function in cultured c

121 The plant homeodomain (PHD) finger is found in many chromatin-associated protei

122 oblem is to predict structural features of a PHD finger knowing only its sequence.

123 nal regulators, including plant homeodomain (PHD)-finger-like domains, and defines a plant-specific p

124 K4me2 recruits the Set3 complex via the Set3 PHD finger, localizing the Hos2 and Hst1 subunits to dea

125 The PHD finger may promote both gene expression and repressi

126 y insertion" (L3MBTL1) and "surface groove" (PHD finger) modes of methyllysine recognition, a carboxy

127 The PHD finger motif is a signature chromatin-associated mot

128 rotein sequence shows strong homology to the PHD-finger motif found in known transcription factors fr

129 tational domain search revealed a C-terminal PHD-finger motif.

130 rmination of the MS1 protein and loss of the PHD-finger motif.

131 Msc1 contains 3 plant homeodomain (PHD) finger motifs, characteristically defined by a C4HC

132 introduction of wild-type BHC80 but not by a PHD-finger mutant that cannot bind H3.

133 ), which bind methylated H3K4 (H3K4me3), the PHD finger of BHC80 binds unmethylated H3K4 (H3K4me0), a

134 The crystal structure of the PHD finger of BHC80 bound to an unmodified H3 peptide ha

135 d NMR structures of the bromodomain-proximal PHD finger of BPTF in free and H3(1-15)K4me3-bound state

136 We show that the second PHD finger of CHD4 initiates recruitment to the nucleoso

137 The second PHD finger of human BPTF is known to specifically recogn

138 Insertion of the third PHD finger of MLL into MLL-ENL allows the recruitment of

139 This activity is conserved in the second PHD finger of MLL4, the closest homolog to MLL1 but not

140 ing PHD finger, such as the carboxy-terminal PHD finger of PHF23 or JARID1A (also known as KDM5A or R

141 one-binding activity is not conserved in the PHD finger of Set4 suggests different functions for the

142 ted screen for PI interactors identified the PHD finger of TAF3, a TATA box binding protein-associate

143 The PHD finger of the RAG2 polypeptide of the RAG1/RAG2 comp

144 0 fusion protein, which lacks the N-terminal PHD fingers of AF10.

145 of acetylated histone binding by the double PHD fingers of DPF3b.

146 We find that the PHD fingers of ING2 and other diverse nuclear proteins b

147 We show that each of the three PHD fingers of Msc1 can act as ubiquitin E3 ligases, rep

148 The function of the PHD fingers of Msc1 is needed to rescue the DNA damage s

149 RPD3 binds directly to PCL via the conserved PHD fingers of PCL and the N terminus of RPD3.

150 The Zn-coordinated PHD fingers of Pygopus (Pygo) proteins are critical for

151 Here, we show that the dual PHD fingers of Rco1, a member of the Rpd3S histone deace

152 erminants of methyllysine recognition by the PHD fingers of Set3 and its orthologs.

153 Here we report that, in contrast to the PHD fingers of the bromodomain PHD finger transcription

154 A pair of readers, the PHD fingers of the protein CHD4, has been shown to bival

155 trimethylated at K4) by a plant homeodomain (PHD) finger of human BPTF (bromodomain and PHD domain tr

156 leoporin 98 and the third plant homeodomain (PHD) finger of JARID1A drives an oncogenic transcription

157 We found that the plant homeodomain (PHD) finger of Jhd2 is important for its chromatin assoc

158 The plant homeodomain (PHD) finger of Set3 binds methylated lysine 4 of histone

159 We recently found that plant homeodomain (PHD) finger of tumour suppressor ING2 (inhibitor of grow

160 a prime example of histone tail binding by a PHD finger (of Pygo) being modulated by a cofactor (BCL9

161 a unique chromatin regulator possessing two PHD fingers, one bromodomain and a PWWP domain for recog

162 Acf1 is a novel protein that contains two PHD fingers, one bromodomain, and two new conserved regi

163 Substitution of highly related PHD fingers or bromodomains failed to restore repression

164 Here we identify the UHRF1 PHD finger (PHD(UHRF1)), an important regulator of DNA C

165 Recently, a tandem plant homeodomain (PHD) finger (PHD1-PHD2, or PHD12) of human DPF3b, which

166 Here we show that the second PHD finger (PHD2) of MLL1 is an E3 ubiquitin ligase in t

167 s from binding studies of H3(1-15)K4me3 with PHD finger point mutants.

168 d long protein containing a SET domain, five PHD fingers, potential zinc fingers, and a very long run

169 istone methyl-lysine binding activity of the PHD fingers present within the Saccharomyces cerevisiae

170 as a quaternary complex with the bromodomain-PHD finger protein 1 (BRPF1), inhibitor of growth 5 (ING

171 Here we show that PHD finger protein 20-like 1 (PHF20L1) regulates DNMT1 t

172 utative human homologs of Bye1, the proteins PHD finger protein 3 and death-inducer obliterator, whic

173 Here we identify PHD Finger Protein 7 (PHF7) as an important factor for m

174 osophila identified pygopus, which encodes a PHD finger protein, as an additional nuclear component o

175 one H3T3/T6 phosphorylation and retention of PHD finger proteins in chromatin during mitosis.

176 transferase complexes, both of which contain PHD finger proteins that bind methylated H3K4.

177 ongs to a family of evolutionarily conserved PHD finger proteins thought to act as co-activators of W

178 Recently, PHD finger proteins, like Yng1 in the NuA3 HAT complex,

179 ecent papers describe how plant homeodomain (PHD) finger proteins read part of this code.

180 mechanisms underlying this novel function of PHD fingers provides a basis for deciphering the role of

181 region consisting of a PHD finger-Zn knuckle-PHD finger (PZP) folds into a single module that recogni

182 The ING1 PHD finger recognizes methylated H3K4 but not other hist

183 Inclusion of 2 or more PHD fingers reduced association with the Hoxa9 locus and

184 itution within the aromatic cage of the BPTF PHD finger, resulting in a reversal of binding preferenc

185 ation of a novel transcript containing SNF2, PHD-finger, RING-finger, helicase, and linker histone do

186 3 and Thr 6 of the peptide, account for the PHD finger's high specificity and affinity.

187 The PHD fingers seemed to be necessary for the formation of

188 r families, providing informative labels for PHD finger sequences.

189 ing an H3K4-trimethylation (H3K4me3)-binding PHD finger, such as the carboxy-terminal PHD finger of P

190 Strong binding of other ING and YNG PHD fingers suggests that the recognition of H3K4me3 his

191 s that the binding of Cyp33 to the MLL third PHD finger switches the MLL function from transactivatio

192 ionarily conserved nuclear protein bearing a PHD finger that is essential for its activity.

193 A point mutation in the p300 PHD finger that is related to the Rubinstein-Taybi syndr

194 In these processes, a PHD finger that specifically recognizes H3K4me3/2 marks

195 Mutations in PHD fingers that abrogated H3K4me3 binding also abolishe

196 gene assembly--contains a plant homeodomain (PHD) finger that specifically recognizes histone H3 trim

197 LL protein contains three plant homeodomain (PHD) fingers that are well conserved between species but

198 nserved modules including plant homeodomain (PHD) fingers that recognize varied H3K4me states.

199 putative double zinc-finger domain, called a PHD finger, that is present not only in the products of

200 BS69 contains regions of similarity to the PHD finger, the bromodomain, and the MYND domain, all of

201 KAP-1 contains a RING finger, B boxes, and a PHD finger; the RING-B1-B2 structure is required for KRA

202 Along with the SET domain and the PHD fingers, this new element is a signature feature for

203 Binding of the RAG-2 PHD finger to chromatin across the IgH D-J(H)-C locus sh

204 olecules that disrupt binding of the JARID1A PHD finger to histone peptides.

205 crocyclic calixarenes can disrupt binding of PHD fingers to methylated lysine 4 of histone H3 in vitr

206 ochemical evidence for the utility of tandem PHD fingers to recruit protein complexes at targeted gen

207 The close structural relationship of PHD fingers to RING fingers suggests that other PHD doma

208 tudy, we demonstrate the ability of the CHD5 PHD fingers to specifically recognize the unmodified N-t

209 stone H3, reduce the binding affinity of the PHD finger toward the histone substrate.

210 ntrast to the PHD fingers of the bromodomain PHD finger transcription factor (BPTF) and inhibitor of

211 Bromodomain PHD finger transcription factor (BPTF) is the largest su

212 Depletion of Bptf (bromodomain PHD finger transcription factor), the largest NURF subun

213 A fusion protein in which the ING2 PHD finger was linked to the LEDGF IBD directed integrat

214 ging the polybasic regions between different PHD fingers we show that this region is a strong determi

215 the molecular basis of the integrated tandem PHD finger, which acts as one functional unit in the seq

216 Among these folds are PHD fingers, which are present in most chromatin modific

217 ins four highly conserved plant homeodomain (PHD) fingers, which are invariably deleted in oncogenic

218 s preceded by a tandem of plant homeodomain (PHD) fingers whose biological roles and requirements for

219 rated a direct interaction of the chick Pcl2 PHD finger with EZH2, a component of the ESC/E(Z) repres

220 mechanism of multivalent association of the PHD fingers with chromatin and reveal their critical rol

221 raction of the C-terminal plant homeodomain (PHD) finger with histone H3 trimethylated at Lys4 (H3K4m

222 l beta-sheet formation on the surface of the PHD finger, with the long side chains of arginine 2 (R2)

223 An AF10 region consisting of a PHD finger-Zn knuckle-PHD finger (PZP) folds into a sing