ライフサイエンスコーパス: PI 4-kinase

1 PI 4-kinase and oxysterol-binding protein (OSBP) were in

2 KDU731, a PI (4) kinase inhibitor, blocks Cryptosporidium infectio

3 -kinase indicating that Lsb6p functions as a PI 4-kinase in vivo.

4 OSBP might also be a PI 4-kinase effector in poliovirus infection and could b

5 We aimed to identify and characterize a PI 4-kinase effector in HCV replication.

6 OSBP was recruited to membranous webs in a PI 4-kinase-dependent manner, and both these factors wer

7 OSBP is a PI 4-kinase effector in HCV infection, and contributes t

8 We report here that a PI 4-kinase (PI4K) activity previously reported on synap

9 mes (a PtdIns(4)P-5-kinase in S. pombe and a PI-4-kinase in D. melanogaster) that interfere with cyto

10 ase, and Vps34p and Pik1p, a PI 3-kinase and PI 4-kinase, respectively--showed not only different PIP

11 with antibodies against either proteins, and PI 4-kinase activity was present in anti-NCS-1 immunopre

12 Lipid phosphorylation by PI 4-kinase is required for recovery from muscarinic mod

13 utant defective in the type III STT4-encoded PI 4-kinase indicating that Lsb6p functions as a PI 4-ki

14 Elevated accumulation of the fourth enzyme, PI 4-kinase, was observed in the fl2 endosperm and soybe

15 ribe a homogeneous and nonisotopic assay for PI 4-kinase activity based on the bioluminescent detecti

16 Our results suggest a critical role for PI 4-kinases and phosphatidylinositol derivatives during

17 Wild-type activity of the fwd PI 4-kinase is required for tyrosine phosphorylation in

18 unoaffinity-purified Arabidopsis protein had PI 4-kinase activity.

19 However, it is not known how PI 4-kinases and their product, PI(4)P, facilitate host

20 % identical and 68% similar to rat and human PI 4-kinases and contained the telltale lipid kinase uni

21 ast, the recent cloning of the first type II PI 4-kinase enzyme defined a novel enzyme family.

22 e the existence of multiple forms of type II PI 4-kinase in mammalian cells and suggest that their fu

23 PIK93 that selectively inhibits the type III PI 4-kinase beta enzyme, and small interfering RNA-media

24 n inhibitor of both PI 3-kinase and type III PI 4-kinase, blocked CaR-stimulated accumulation of [(3)

25 ly membrane-associated WT-sensitive type III PI 4-kinases were isolated from bovine adrenal cortex as

26 cloning of these novel WT-sensitive type III PI 4-kinases will allow detailed analysis of their signa

27 on, whereas co-transfection with an inactive PI 4-kinase mutant prevented the NCS-1-induced inhibitio

28 Expression of both enzyme yields increased PI 4-kinase activity that is associated with the microso

29 A-mediated down-regulation of the individual PI 4-kinase enzymes, revealed that PI 4-kinase beta has

30 sed on the findings that CaR and the 110-kDa PI 4-kinase beta can be co-immunoprecipitated with antib

31 to those of a recently described rat 230-kDa PI 4-kinase.

32 ry similar to the membrane-associated 55-kDa PI 4-kinase previously purified from S. cerevisiae.

33 CS-1 effector phosphatidylinositol 4-kinase (PI 4-kinase) inhibited insulin-stimulated GLUT4 transloc

34 n to encode a phosphatidylinositol 4-kinase (PI 4-kinase), a member of a family of proteins that perf

35 idence that a phosphatidylinositol 4-kinase (PI 4-kinase), STT4, is a target of wortmannin in yeast.

36 Phosphatidylinositol 4-kinases (PI 4-kinases) catalyze the conversion of phosphatidylino

37 ide to determine which of the four mammalian PI 4-kinases are involved in this process.

38 The four known mammalian PI 4-kinases, PI4KA, PI4KB, PI4K2A, and PI4K2B have role

39 Two mammalian PI 4-kinases have been cloned, a 230-kDa enzyme (alpha-f

40 tous growth, despite uniform plasma membrane PI-4-kinase distribution.

41 te 5 kinase, are phenocopied by knockdown of PI 4 kinase, and are associated with normal endoplasmic

42 Accordingly, inhibition of PI 4-kinase III beta either by wortmannin or PIK93 inhib

43 (3)H]PIP formation, reflecting inhibition of PI 4-kinase.

44 lacking the LSB6 gene had a reduced level of PI 4-kinase activity.

45 ution in COS-7 cells during manipulations of PI 4-kinase (PI4K) activities.

46 the LSB6 gene directed the overexpression of PI 4-kinase activity in cell extracts of wild-type cells

47 her to the type II or the type III family of PI 4-kinases.

48 assay with known nonselective inhibitors of PI 4-kinases and show that it performs similar to radiom

49 tial cDNAs of the >7-kilobase transcripts of PI 4-kinases from carrot (DcPI4Kalpha) and Arabidopsis (

50 itated with antibodies against either CaR or PI 4-kinase.

51 of PIP(2) synthesis by phosphatidylinositol (PI) 4-kinase.

52 ber of a novel type II phosphatidylinositol (PI) 4-kinase family.

53 und that CaR regulates phosphatidylinositol (PI) 4-kinase, the first step in inositol lipid biosynthe

54 tmannin (WT)-sensitive phosphatidylinositol (PI) 4-kinase(s).

55 Phosphatidylinositol (PI) 4-kinases catalyze the synthesis of PI 4-phosphate,

56 f large molecular mass phosphatidylinositol (PI) 4-kinases.

57 C virus (HCV), require phosphatidylinositol (PI) 4-kinases for their replication.

58 sphate suggesting that phosphatidylinositol (PI) 4-kinases are involved in the regulation of CERT-med

59 n the expression of a protein that possessed PI 4-kinase activity.

60 In this study, the recombinant PI 4-kinase PH domain was explored for its ability to bi

61 tance, but overexpression of PIK1, a related PI 4-kinase, does not.

62 in replication of other viruses that require PI 4-kinases.

63 yielded a 110-kDa protein with WT-sensitive PI 4-kinase activity.

64 er, our data demonstrate that CaR stimulates PI 4-kinase, the first step in inositol lipid biosynthes

65 These data demonstrate that PI 4-kinase functions to negatively regulate GLUT4 trans

66 ndividual PI 4-kinase enzymes, revealed that PI 4-kinase beta has a dominant role in ceramide transpo

67 ation and function of Stt4/PI4KIIIalpha, the PI 4-kinase responsible for this PI4P pool.

68 relates with PKC activation and requires the PI 4-kinase Stt4p.

69 In vitro, the PI 4-kinase activity of STT4, but not of PIK1, was poten

70 The PI-4 kinase in co-immunoprecipitates with anti-CaR antib

71 Therefore, PI 4-kinase beta is a key enzyme in the control of sping

72 inositol lipid synthesis was attributable to PI 4-kinase and not PI 3-kinase because CaR did not acti

73 Similar measurements using PI 4-kinase showed a weak dependence on vesicle size.

74 However, when PI 4-kinase IIIbeta, diacylglycerol kinase, Rho, or Rho-

75 Here, we investigate which PI 4-kinase isoform underlies this signal, whether stimu

76 CaR associates with PI 4-kinase based on the findings that CaR and the 110-k

77 One-step treatment with PI 4-kinase IIIbeta (PI4Kbeta) yielded PI 4-phosphate (P